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Protein

Recombination-promoting nuclease RpnC

Gene

rpnC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

A low activity DNA endonuclease yielding 3'-hydroxyl ends (Probable). Upon expression enhances RecA-independent DNA recombination 2.9-fold, concomitantly reducing viability by 59% and inducing DNA damage as measured by induction of the SOS repair response.1 Publication

GO - Molecular functioni

GO - Biological processi

  • DNA recombination Source: UniProtKB

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA recombination
LigandMagnesium

Enzyme and pathway databases

BioCyciEcoCyc:EG11748-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Recombination-promoting nuclease RpnC1 Publication (EC:3.1.21.-)
Gene namesi
Name:rpnC1 Publication
Synonyms:yadD
Ordered Locus Names:b0132, JW5010
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11748. yadD.

Pathology & Biotechi

Disruption phenotypei

A quadruple rpnA-rpnB-rpnC-rpnD deletion shows no change in basal RecA-independent recombination.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001685261 – 300Recombination-promoting nuclease RpnCAdd BLAST300

Proteomic databases

PaxDbiP31665.
PRIDEiP31665.

Interactioni

Protein-protein interaction databases

BioGridi4260707. 246 interactors.
STRINGi316385.ECDH10B_0112.

Structurei

3D structure databases

ProteinModelPortaliP31665.
SMRiP31665.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Rpn/YhgA-like nuclease family.Curated

Phylogenomic databases

eggNOGiENOG4108A32. Bacteria.
COG5464. LUCA.
HOGENOMiHOG000116866.
InParanoidiP31665.
PhylomeDBiP31665.

Family and domain databases

InterProiView protein in InterPro
IPR010106. RpnA.
IPR006842. Transposase_31.
PfamiView protein in Pfam
PF04754. Transposase_31. 1 hit.
TIGRFAMsiTIGR01784. T_den_put_tspse. 1 hit.

Sequencei

Sequence statusi: Complete.

P31665-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAPSTTPHD AVFKQFLMHA ETARDFLEIH LPVELRELCD LNTLHLESGS
60 70 80 90 100
FIEESLKGHS TDVLYSVQMQ GNPGYLHVVI EHQSKPDKKM AFRMMRYSIA
110 120 130 140 150
AMHRHLEADH DKLPLVVPIL FYQGEATPYP LSMCWFDMFY SPELARRVYN
160 170 180 190 200
SPFPLVDITI TPDDEIMQHR RIAILELLQK HIRQRDLMLL LEQLVTLIDE
210 220 230 240 250
GYTSGSQLVA MQNYMLQRGH TEQADLFYGV LRDRETGGES MMTLAQWFEE
260 270 280 290 300
KGIEKGIQQG RQEVSQEFAQ RLLSKGMSRE DVAEMANLPL AEIDKVINLI
Length:300
Mass (Da):34,607
Last modified:November 1, 1997 - v2
Checksum:iF551985238862C65
GO

Sequence cautioni

The sequence AAA24273 differs from that shown. Reason: Frameshift at position 17.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L17086 Genomic DNA. Translation: AAA24273.1. Frameshift.
U00096 Genomic DNA. Translation: AAC73243.1.
AP009048 Genomic DNA. Translation: BAB96710.2.
PIRiD64736.
RefSeqiNP_414674.1. NC_000913.3.
WP_000339954.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73243; AAC73243; b0132.
BAB96710; BAB96710; BAB96710.
GeneIDi944781.
KEGGiecj:JW5010.
eco:b0132.
PATRICifig|1411691.4.peg.2149.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRPNC_ECOLI
AccessioniPrimary (citable) accession number: P31665
Secondary accession number(s): P75660, Q8KJQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: July 5, 2017
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families