Sodium-dependent neutral amino acid transporter SLC6A17

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P31662 (S6A17_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sodium-dependent neutral amino acid transporter SLC6A17

Alternative name(s):
Sodium-dependent neurotransmitter transporter NTT4
Solute carrier family 6 member 17

Gene names

Name:	Slc6a17
Synonyms:	Ntt4, Rxt1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a sodium-dependent vesicular transporter selective for proline, glycine, leucine and alanine. In contrast to other members of this neurotransmitter transporter family, does not appear to be chloride-dependent. Ref.4
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Multi-pass membrane protein Ref.4 Ref.5.
Tissue specificity	Found exclusively in the central nervous system and is more abundant in the cerebellum and the cerebral cortex. Expressed in PC-12 cell line. Ref.3 Ref.4
Sequence similarities	Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A17 subfamily. [View classification]
Biophysicochemical properties	Kinetic parameters: K_M=1.72 mM for glycine Ref.4 K_M=0.86 mM for proline V_max=199 pmol/min/mg enzyme for glycine V_max=172 pmol/min/mg enzyme for proline pH dependence: Optimum pH is 8.5.

Ontologies

Keywords
Biological process	Neurotransmitter transport Symport Transport
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	alanine transport Inferred from direct assay Ref.4. Source: UniProtKB glycine transport Inferred from direct assay Ref.4. Source: UniProtKB leucine transport Inferred from direct assay Ref.4. Source: UniProtKB proline transport Inferred from direct assay Ref.4 Ref.5. Source: UniProtKB
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Inferred from electronic annotation. Source: InterPro synaptic vesicle Inferred from direct assay Ref.4 Ref.5. Source: UniProtKB synaptic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	neurotransmitter:sodium symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 727

727

Sodium-dependent neutral amino acid transporter SLC6A17

PRO_0000214805

Regions

Topological domain

1 – 68

Cytoplasmic Potential

Transmembrane

69 – 89

Helical; Name=1; Potential

Topological domain

90 – 96

Extracellular Potential

Transmembrane

97 – 116

Helical; Name=2; Potential

Topological domain

117 – 140

Cytoplasmic Potential

Transmembrane

141 – 161

Helical; Name=3; Potential

Topological domain

162 – 224

Extracellular Potential

Transmembrane

225 – 243

Helical; Name=4; Potential

Topological domain

244 – 251

Cytoplasmic Potential

Transmembrane

252 – 269

Helical; Name=5; Potential

Topological domain

270 – 304

Extracellular Potential

Transmembrane

305 – 322

Helical; Name=6; Potential

Topological domain

323 – 333

Cytoplasmic Potential

Transmembrane

334 – 355

Helical; Name=7; Potential

Topological domain

356 – 451

Extracellular Potential

Transmembrane

452 – 471

Helical; Name=8; Potential

Topological domain

472 – 494

Cytoplasmic Potential

Transmembrane

495 – 513

Helical; Name=9; Potential

Topological domain

514 – 528

Extracellular Potential

Transmembrane

529 – 549

Helical; Name=10; Potential

Topological domain

550 – 569

Cytoplasmic Potential

Transmembrane

570 – 591

Helical; Name=11; Potential

Topological domain

592 – 618

Extracellular Potential

Transmembrane

619 – 641

Helical; Name=12; Potential

Topological domain

642 – 727

Cytoplasmic Potential

Amino acid modifications

Modified residue

377

Phosphotyrosine By similarity

Modified residue

665

Phosphoserine By similarity

Modified residue

700

Phosphothreonine By similarity

Modified residue

722

Phosphothreonine By similarity

Glycosylation

186

N-linked (GlcNAc...) Potential

Glycosylation

393

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

261

Y → C in AAC60673. Ref.2

Sequence conflict

499

G → S in AAC60673. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P31662 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: C676048C0A6BDF7C
Show »

FASTA

727

81,055

        10         20         30         40         50         60 
MPKNSKVTQR EHSNEHVTES VADLLALEEP VDYKQSVLNV AGETGGKQKV AEEELDAEDR 

        70         80         90        100        110        120 
PAWNSKLQYI LAQIGFSVGL GNIWRFPYLC QKNGGGAYLV PYLVLLIIIG IPLFFLELAV 

       130        140        150        160        170        180 
GQRIRRGSIG VWHYVCPRLG GIGFSSCIVC LFVGLYYNVI IGWSVFYFFK SFQYPLPWSE 

       190        200        210        220        230        240 
CPVIRNGTVA VVEPECEKSS ATTYFWYREA LDISNSISES GGLNWKMTVC LLVAWSIVGM 

       250        260        270        280        290        300 
AVVKGIQSSG KVMYFSSLFP YVVLACFLVR GLLLRGAVDG ILHMFTPKLD KMLDPQVWRE 

       310        320        330        340        350        360 
AATQVFFALG LGFGGVIAFS SYNKQDNNCH FDAALVSFIN FFTSVLATLV VFAVLGFKAN 

       370        380        390        400        410        420 
IMNEKCVVEN AEKILGYLNS NVLSRDLIPP HVNFSHLTTK DYSEMYNVIM TVKEKQFSAL 

       430        440        450        460        470        480 
GLDPCLLEDE LDKSVQGTGL AFIAFTEAMT HFPASPFWSV MFFLMLINLG LGSMIGTMAG 

       490        500        510        520        530        540 
ITTPIIDTFK VPKEMFTVGC CVFAFFVGLL FVQRSGNYFV TMFDDYSATL PLTVIVILEN 

       550        560        570        580        590        600 
IAVAWIYGTK KFMQELTEML GFRPYRFYFY MWKFVSPLCM AVLTTASIIQ LGVSPPGYSA 

       610        620        630        640        650        660 
WIKEEAAERY LYFPNWAMAL LITLIAVATL PIPVVFILRH FHLLSDGSNT LSVSYKKGRM 

       670        680        690        700        710        720 
MKDISNLEEN DETRFILSKV PSEAPSPMPT HRSYLGPGST SPLESSSHPN GRYGSGYLLA 


STPESEL

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References

[1]	"A rat brain cDNA encoding the neurotransmitter transporter with an unusual structure." Liu Q.-R., Mandiyan S., Lopez-Corcuera B., Nelson H., Nelson N. FEBS Lett. 315:114-118(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Characterization of an atypical member of the Na+/Cl(-)-dependent transporter family: chromosomal localization and distribution in GABAergic and glutamatergic neurons in the rat brain." el Mestikawy S., Giros B., Pohl M., Hamon M., Kingsmore S.F., Seldin M.F., Caron M.G. J. Neurochem. 62:445-455(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The repertoire of solute carriers of family 6: identification of new human and rodent genes." Hoglund P.J., Adzic D., Scicluna S.J., Lindblom J., Fredriksson R. Biochem. Biophys. Res. Commun. 336:175-189(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[4]	"The orphan transporter Rxt1/NTT4 (SLC6A17) functions as a synaptic vesicle amino acid transporter selective for proline, glycine, leucine, and alanine." Parra L.A., Baust T., El Mestikawy S., Quiroz M., Hoffman B., Haflett J.M., Yao J.K., Torres G.E. Mol. Pharmacol. 74:1521-1532(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[5]	"Synaptic vesicle protein NTT4/XT1 (SLC6A17) catalyzes Na+-coupled neutral amino acid transport." Zaia K.A., Reimer R.J. J. Biol. Chem. 284:8439-8448(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, PH DEPENDENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L06434 mRNA. Translation: AAB24776.1. S68944 mRNA. Translation: AAC60673.1.
IPI	IPI00214498.
PIR	I56506. S27043.
RefSeq	NP_001028251.1. NM_001033079.1.
UniGene	Rn.186904.
3D structure databases
ProteinModelPortal	P31662.
ModBase	Search...
Protein family/group databases
TCDB	2.A.22.6.2. neurotransmitter:sodium symporter (NSS) family.
PTM databases
PhosphoSite	P31662.
Proteomic databases
PRIDE	P31662.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000075653; ENSRNOP00000065179; ENSRNOG00000050090.
GeneID	613226.
KEGG	rno:613226.
Organism-specific databases
CTD	388662.
RGD	1587185. Slc6a17.
Phylogenomic databases
GeneTree	ENSGT00660000095315.
HOVERGEN	HBG071421.
KO	K05048.
Gene expression databases
Genevestigator	P31662.
Family and domain databases
InterPro	IPR000175. Na/ntran_symport. IPR002438. Na/ntran_symport_orphan. [Graphical view]
PANTHER	PTHR11616. PTHR11616. 1 hit.
Pfam	PF00209. SNF. 1 hit. [Graphical view]
PRINTS	PR00176. NANEUSMPORT. PR01206. ORPHTRNSPORT.
PROSITE	PS00610. NA_NEUROTRAN_SYMP_1. 1 hit. PS00754. NA_NEUROTRAN_SYMP_2. 1 hit. PS50267. NA_NEUROTRAN_SYMP_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	714599.

Entry information

Entry name

S6A17_RAT

Accession

Primary (citable) accession number: P31662

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents