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Protein

Sodium-dependent neutral amino acid transporter SLC6A17

Gene

Slc6a17

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a sodium-dependent vesicular transporter selective for proline, glycine, leucine and alanine. In contrast to other members of this neurotransmitter transporter family, does not appear to be chloride-dependent.1 Publication

Kineticsi

  1. KM=1.72 mM for glycine1 Publication
  2. KM=0.86 mM for proline1 Publication

Vmax=199 pmol/min/mg enzyme for glycine1 Publication

Vmax=172 pmol/min/mg enzyme for proline1 Publication

pH dependencei

Optimum pH is 8.5.2 Publications

GO - Molecular functioni

  1. neurotransmitter:sodium symporter activity Source: InterPro

GO - Biological processi

  1. alanine transport Source: UniProtKB
  2. glycine transport Source: UniProtKB
  3. leucine transport Source: UniProtKB
  4. neutral amino acid transport Source: UniProtKB
  5. proline transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Symport, Transport

Protein family/group databases

TCDBi2.A.22.6.2. the neurotransmitter:sodium symporter (nss) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent neutral amino acid transporter SLC6A17
Alternative name(s):
Sodium-dependent neurotransmitter transporter NTT4
Solute carrier family 6 member 17
Gene namesi
Name:Slc6a17
Synonyms:Ntt4, Rxt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1587185. Slc6a17.

Subcellular locationi

  1. Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane 2 Publications; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6868CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei69 – 8921Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini90 – 967ExtracellularSequence Analysis
Transmembranei97 – 11620Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini117 – 14024CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei141 – 16121Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini162 – 22463ExtracellularSequence AnalysisAdd
BLAST
Transmembranei225 – 24319Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini244 – 2518CytoplasmicSequence Analysis
Transmembranei252 – 26918Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini270 – 30435ExtracellularSequence AnalysisAdd
BLAST
Transmembranei305 – 32218Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini323 – 33311CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei334 – 35522Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini356 – 45196ExtracellularSequence AnalysisAdd
BLAST
Transmembranei452 – 47120Helical; Name=8Sequence AnalysisAdd
BLAST
Topological domaini472 – 49423CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei495 – 51319Helical; Name=9Sequence AnalysisAdd
BLAST
Topological domaini514 – 52815ExtracellularSequence AnalysisAdd
BLAST
Transmembranei529 – 54921Helical; Name=10Sequence AnalysisAdd
BLAST
Topological domaini550 – 56920CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei570 – 59122Helical; Name=11Sequence AnalysisAdd
BLAST
Topological domaini592 – 61827ExtracellularSequence AnalysisAdd
BLAST
Transmembranei619 – 64123Helical; Name=12Sequence AnalysisAdd
BLAST
Topological domaini642 – 72786CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. integral component of plasma membrane Source: InterPro
  3. synaptic vesicle Source: UniProtKB
  4. synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727Sodium-dependent neutral amino acid transporter SLC6A17PRO_0000214805Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis
Modified residuei377 – 3771PhosphotyrosineBy similarity
Glycosylationi393 – 3931N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP31662.

PTM databases

PhosphoSiteiP31662.

Expressioni

Tissue specificityi

Found exclusively in the central nervous system and is more abundant in the cerebellum and the cerebral cortex. Expressed in PC-12 cell line.2 Publications

Gene expression databases

GenevestigatoriP31662.

Interactioni

Protein-protein interaction databases

BioGridi565981. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP31662.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119044.
HOVERGENiHBG071421.
InParanoidiP31662.
KOiK05048.
OMAiMEMYNVI.
OrthoDBiEOG793B71.
PhylomeDBiP31662.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002438. Na/ntran_symport_orphan.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PR01206. ORPHTRNSPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31662-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKNSKVTQR EHSNEHVTES VADLLALEEP VDYKQSVLNV AGETGGKQKV
60 70 80 90 100
AEEELDAEDR PAWNSKLQYI LAQIGFSVGL GNIWRFPYLC QKNGGGAYLV
110 120 130 140 150
PYLVLLIIIG IPLFFLELAV GQRIRRGSIG VWHYVCPRLG GIGFSSCIVC
160 170 180 190 200
LFVGLYYNVI IGWSVFYFFK SFQYPLPWSE CPVIRNGTVA VVEPECEKSS
210 220 230 240 250
ATTYFWYREA LDISNSISES GGLNWKMTVC LLVAWSIVGM AVVKGIQSSG
260 270 280 290 300
KVMYFSSLFP YVVLACFLVR GLLLRGAVDG ILHMFTPKLD KMLDPQVWRE
310 320 330 340 350
AATQVFFALG LGFGGVIAFS SYNKQDNNCH FDAALVSFIN FFTSVLATLV
360 370 380 390 400
VFAVLGFKAN IMNEKCVVEN AEKILGYLNS NVLSRDLIPP HVNFSHLTTK
410 420 430 440 450
DYSEMYNVIM TVKEKQFSAL GLDPCLLEDE LDKSVQGTGL AFIAFTEAMT
460 470 480 490 500
HFPASPFWSV MFFLMLINLG LGSMIGTMAG ITTPIIDTFK VPKEMFTVGC
510 520 530 540 550
CVFAFFVGLL FVQRSGNYFV TMFDDYSATL PLTVIVILEN IAVAWIYGTK
560 570 580 590 600
KFMQELTEML GFRPYRFYFY MWKFVSPLCM AVLTTASIIQ LGVSPPGYSA
610 620 630 640 650
WIKEEAAERY LYFPNWAMAL LITLIAVATL PIPVVFILRH FHLLSDGSNT
660 670 680 690 700
LSVSYKKGRM MKDISNLEEN DETRFILSKV PSEAPSPMPT HRSYLGPGST
710 720
SPLESSSHPN GRYGSGYLLA STPESEL
Length:727
Mass (Da):81,055
Last modified:July 1, 1993 - v1
Checksum:iC676048C0A6BDF7C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti261 – 2611Y → C in AAC60673 (PubMed:8294906).Curated
Sequence conflicti499 – 4991G → S in AAC60673 (PubMed:8294906).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06434 mRNA. Translation: AAB24776.1.
S68944 mRNA. Translation: AAC60673.1.
PIRiI56506.
S27043.
RefSeqiNP_001028251.1. NM_001033079.1.
UniGeneiRn.186904.

Genome annotation databases

EnsembliENSRNOT00000075653; ENSRNOP00000065179; ENSRNOG00000050090.
GeneIDi613226.
KEGGirno:613226.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L06434 mRNA. Translation: AAB24776.1.
S68944 mRNA. Translation: AAC60673.1.
PIRiI56506.
S27043.
RefSeqiNP_001028251.1. NM_001033079.1.
UniGeneiRn.186904.

3D structure databases

ProteinModelPortaliP31662.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi565981. 1 interaction.

Protein family/group databases

TCDBi2.A.22.6.2. the neurotransmitter:sodium symporter (nss) family.

PTM databases

PhosphoSiteiP31662.

Proteomic databases

PRIDEiP31662.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000075653; ENSRNOP00000065179; ENSRNOG00000050090.
GeneIDi613226.
KEGGirno:613226.

Organism-specific databases

CTDi388662.
RGDi1587185. Slc6a17.

Phylogenomic databases

GeneTreeiENSGT00760000119044.
HOVERGENiHBG071421.
InParanoidiP31662.
KOiK05048.
OMAiMEMYNVI.
OrthoDBiEOG793B71.
PhylomeDBiP31662.

Miscellaneous databases

NextBioi714599.
PROiP31662.

Gene expression databases

GenevestigatoriP31662.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002438. Na/ntran_symport_orphan.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PR01206. ORPHTRNSPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A rat brain cDNA encoding the neurotransmitter transporter with an unusual structure."
    Liu Q.-R., Mandiyan S., Lopez-Corcuera B., Nelson H., Nelson N.
    FEBS Lett. 315:114-118(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Characterization of an atypical member of the Na+/Cl(-)-dependent transporter family: chromosomal localization and distribution in GABAergic and glutamatergic neurons in the rat brain."
    el Mestikawy S., Giros B., Pohl M., Hamon M., Kingsmore S.F., Seldin M.F., Caron M.G.
    J. Neurochem. 62:445-455(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The repertoire of solute carriers of family 6: identification of new human and rodent genes."
    Hoglund P.J., Adzic D., Scicluna S.J., Lindblom J., Fredriksson R.
    Biochem. Biophys. Res. Commun. 336:175-189(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
  4. "The orphan transporter Rxt1/NTT4 (SLC6A17) functions as a synaptic vesicle amino acid transporter selective for proline, glycine, leucine, and alanine."
    Parra L.A., Baust T., El Mestikawy S., Quiroz M., Hoffman B., Haflett J.M., Yao J.K., Torres G.E.
    Mol. Pharmacol. 74:1521-1532(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  5. "Synaptic vesicle protein NTT4/XT1 (SLC6A17) catalyzes Na+-coupled neutral amino acid transport."
    Zaia K.A., Reimer R.J.
    J. Biol. Chem. 284:8439-8448(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PH DEPENDENCE.

Entry informationi

Entry nameiS6A17_RAT
AccessioniPrimary (citable) accession number: P31662
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: March 4, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.