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P31662

- S6A17_RAT

UniProt

P31662 - S6A17_RAT

Protein

Sodium-dependent neutral amino acid transporter SLC6A17

Gene

Slc6a17

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Functions as a sodium-dependent vesicular transporter selective for proline, glycine, leucine and alanine. In contrast to other members of this neurotransmitter transporter family, does not appear to be chloride-dependent.1 Publication

    Kineticsi

    1. KM=1.72 mM for glycine1 Publication
    2. KM=0.86 mM for proline1 Publication

    Vmax=199 pmol/min/mg enzyme for glycine1 Publication

    Vmax=172 pmol/min/mg enzyme for proline1 Publication

    pH dependencei

    Optimum pH is 8.5.2 Publications

    GO - Molecular functioni

    1. neurotransmitter:sodium symporter activity Source: InterPro

    GO - Biological processi

    1. alanine transport Source: UniProtKB
    2. glycine transport Source: UniProtKB
    3. leucine transport Source: UniProtKB
    4. neutral amino acid transport Source: UniProtKB
    5. proline transport Source: UniProtKB

    Keywords - Biological processi

    Neurotransmitter transport, Symport, Transport

    Protein family/group databases

    TCDBi2.A.22.6.2. the neurotransmitter:sodium symporter (nss) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-dependent neutral amino acid transporter SLC6A17
    Alternative name(s):
    Sodium-dependent neurotransmitter transporter NTT4
    Solute carrier family 6 member 17
    Gene namesi
    Name:Slc6a17
    Synonyms:Ntt4, Rxt1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi1587185. Slc6a17.

    Subcellular locationi

    Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. integral component of plasma membrane Source: InterPro
    3. synaptic vesicle Source: UniProtKB
    4. synaptic vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cytoplasmic vesicle, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 727727Sodium-dependent neutral amino acid transporter SLC6A17PRO_0000214805Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis
    Modified residuei377 – 3771PhosphotyrosineBy similarity
    Glycosylationi393 – 3931N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiP31662.

    PTM databases

    PhosphoSiteiP31662.

    Expressioni

    Tissue specificityi

    Found exclusively in the central nervous system and is more abundant in the cerebellum and the cerebral cortex. Expressed in PC-12 cell line.2 Publications

    Gene expression databases

    GenevestigatoriP31662.

    Interactioni

    Protein-protein interaction databases

    BioGridi565981. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP31662.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6868CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini90 – 967ExtracellularSequence Analysis
    Topological domaini117 – 14024CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini162 – 22463ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini244 – 2518CytoplasmicSequence Analysis
    Topological domaini270 – 30435ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini323 – 33311CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini356 – 45196ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini472 – 49423CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini514 – 52815ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini550 – 56920CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini592 – 61827ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini642 – 72786CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei69 – 8921Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei97 – 11620Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei141 – 16121Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei225 – 24319Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei252 – 26918Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei305 – 32218Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei334 – 35522Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei452 – 47120Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei495 – 51319Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei529 – 54921Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei570 – 59122Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei619 – 64123Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00750000117287.
    HOVERGENiHBG071421.
    KOiK05048.
    OMAiMEMYNVI.
    OrthoDBiEOG793B71.
    PhylomeDBiP31662.

    Family and domain databases

    InterProiIPR000175. Na/ntran_symport.
    IPR002438. Na/ntran_symport_orphan.
    [Graphical view]
    PANTHERiPTHR11616. PTHR11616. 1 hit.
    PfamiPF00209. SNF. 1 hit.
    [Graphical view]
    PRINTSiPR00176. NANEUSMPORT.
    PR01206. ORPHTRNSPORT.
    PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31662-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPKNSKVTQR EHSNEHVTES VADLLALEEP VDYKQSVLNV AGETGGKQKV    50
    AEEELDAEDR PAWNSKLQYI LAQIGFSVGL GNIWRFPYLC QKNGGGAYLV 100
    PYLVLLIIIG IPLFFLELAV GQRIRRGSIG VWHYVCPRLG GIGFSSCIVC 150
    LFVGLYYNVI IGWSVFYFFK SFQYPLPWSE CPVIRNGTVA VVEPECEKSS 200
    ATTYFWYREA LDISNSISES GGLNWKMTVC LLVAWSIVGM AVVKGIQSSG 250
    KVMYFSSLFP YVVLACFLVR GLLLRGAVDG ILHMFTPKLD KMLDPQVWRE 300
    AATQVFFALG LGFGGVIAFS SYNKQDNNCH FDAALVSFIN FFTSVLATLV 350
    VFAVLGFKAN IMNEKCVVEN AEKILGYLNS NVLSRDLIPP HVNFSHLTTK 400
    DYSEMYNVIM TVKEKQFSAL GLDPCLLEDE LDKSVQGTGL AFIAFTEAMT 450
    HFPASPFWSV MFFLMLINLG LGSMIGTMAG ITTPIIDTFK VPKEMFTVGC 500
    CVFAFFVGLL FVQRSGNYFV TMFDDYSATL PLTVIVILEN IAVAWIYGTK 550
    KFMQELTEML GFRPYRFYFY MWKFVSPLCM AVLTTASIIQ LGVSPPGYSA 600
    WIKEEAAERY LYFPNWAMAL LITLIAVATL PIPVVFILRH FHLLSDGSNT 650
    LSVSYKKGRM MKDISNLEEN DETRFILSKV PSEAPSPMPT HRSYLGPGST 700
    SPLESSSHPN GRYGSGYLLA STPESEL 727
    Length:727
    Mass (Da):81,055
    Last modified:July 1, 1993 - v1
    Checksum:iC676048C0A6BDF7C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti261 – 2611Y → C in AAC60673. (PubMed:8294906)Curated
    Sequence conflicti499 – 4991G → S in AAC60673. (PubMed:8294906)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06434 mRNA. Translation: AAB24776.1.
    S68944 mRNA. Translation: AAC60673.1.
    PIRiI56506.
    S27043.
    RefSeqiNP_001028251.1. NM_001033079.1.
    UniGeneiRn.186904.

    Genome annotation databases

    EnsembliENSRNOT00000075653; ENSRNOP00000065179; ENSRNOG00000050090.
    GeneIDi613226.
    KEGGirno:613226.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L06434 mRNA. Translation: AAB24776.1 .
    S68944 mRNA. Translation: AAC60673.1 .
    PIRi I56506.
    S27043.
    RefSeqi NP_001028251.1. NM_001033079.1.
    UniGenei Rn.186904.

    3D structure databases

    ProteinModelPortali P31662.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 565981. 1 interaction.

    Protein family/group databases

    TCDBi 2.A.22.6.2. the neurotransmitter:sodium symporter (nss) family.

    PTM databases

    PhosphoSitei P31662.

    Proteomic databases

    PRIDEi P31662.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000075653 ; ENSRNOP00000065179 ; ENSRNOG00000050090 .
    GeneIDi 613226.
    KEGGi rno:613226.

    Organism-specific databases

    CTDi 388662.
    RGDi 1587185. Slc6a17.

    Phylogenomic databases

    GeneTreei ENSGT00750000117287.
    HOVERGENi HBG071421.
    KOi K05048.
    OMAi MEMYNVI.
    OrthoDBi EOG793B71.
    PhylomeDBi P31662.

    Miscellaneous databases

    NextBioi 714599.
    PROi P31662.

    Gene expression databases

    Genevestigatori P31662.

    Family and domain databases

    InterProi IPR000175. Na/ntran_symport.
    IPR002438. Na/ntran_symport_orphan.
    [Graphical view ]
    PANTHERi PTHR11616. PTHR11616. 1 hit.
    Pfami PF00209. SNF. 1 hit.
    [Graphical view ]
    PRINTSi PR00176. NANEUSMPORT.
    PR01206. ORPHTRNSPORT.
    PROSITEi PS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A rat brain cDNA encoding the neurotransmitter transporter with an unusual structure."
      Liu Q.-R., Mandiyan S., Lopez-Corcuera B., Nelson H., Nelson N.
      FEBS Lett. 315:114-118(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Characterization of an atypical member of the Na+/Cl(-)-dependent transporter family: chromosomal localization and distribution in GABAergic and glutamatergic neurons in the rat brain."
      el Mestikawy S., Giros B., Pohl M., Hamon M., Kingsmore S.F., Seldin M.F., Caron M.G.
      J. Neurochem. 62:445-455(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The repertoire of solute carriers of family 6: identification of new human and rodent genes."
      Hoglund P.J., Adzic D., Scicluna S.J., Lindblom J., Fredriksson R.
      Biochem. Biophys. Res. Commun. 336:175-189(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
    4. "The orphan transporter Rxt1/NTT4 (SLC6A17) functions as a synaptic vesicle amino acid transporter selective for proline, glycine, leucine, and alanine."
      Parra L.A., Baust T., El Mestikawy S., Quiroz M., Hoffman B., Haflett J.M., Yao J.K., Torres G.E.
      Mol. Pharmacol. 74:1521-1532(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    5. "Synaptic vesicle protein NTT4/XT1 (SLC6A17) catalyzes Na+-coupled neutral amino acid transport."
      Zaia K.A., Reimer R.J.
      J. Biol. Chem. 284:8439-8448(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, PH DEPENDENCE.

    Entry informationi

    Entry nameiS6A17_RAT
    AccessioniPrimary (citable) accession number: P31662
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3