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P31662 (S6A17_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium-dependent neutral amino acid transporter SLC6A17
Alternative name(s):
Sodium-dependent neurotransmitter transporter NTT4
Solute carrier family 6 member 17
Gene names
Name:Slc6a17
Synonyms:Ntt4, Rxt1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length727 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a sodium-dependent vesicular transporter selective for proline, glycine, leucine and alanine. In contrast to other members of this neurotransmitter transporter family, does not appear to be chloride-dependent. Ref.4

Subcellular location

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Multi-pass membrane protein Ref.4 Ref.5.

Tissue specificity

Found exclusively in the central nervous system and is more abundant in the cerebellum and the cerebral cortex. Expressed in PC-12 cell line. Ref.3 Ref.4

Sequence similarities

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A17 subfamily. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=1.72 mM for glycine Ref.4

KM=0.86 mM for proline

Vmax=199 pmol/min/mg enzyme for glycine

Vmax=172 pmol/min/mg enzyme for proline

pH dependence:

Optimum pH is 8.5.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 727727Sodium-dependent neutral amino acid transporter SLC6A17
PRO_0000214805

Regions

Topological domain1 – 6868Cytoplasmic Potential
Transmembrane69 – 8921Helical; Name=1; Potential
Topological domain90 – 967Extracellular Potential
Transmembrane97 – 11620Helical; Name=2; Potential
Topological domain117 – 14024Cytoplasmic Potential
Transmembrane141 – 16121Helical; Name=3; Potential
Topological domain162 – 22463Extracellular Potential
Transmembrane225 – 24319Helical; Name=4; Potential
Topological domain244 – 2518Cytoplasmic Potential
Transmembrane252 – 26918Helical; Name=5; Potential
Topological domain270 – 30435Extracellular Potential
Transmembrane305 – 32218Helical; Name=6; Potential
Topological domain323 – 33311Cytoplasmic Potential
Transmembrane334 – 35522Helical; Name=7; Potential
Topological domain356 – 45196Extracellular Potential
Transmembrane452 – 47120Helical; Name=8; Potential
Topological domain472 – 49423Cytoplasmic Potential
Transmembrane495 – 51319Helical; Name=9; Potential
Topological domain514 – 52815Extracellular Potential
Transmembrane529 – 54921Helical; Name=10; Potential
Topological domain550 – 56920Cytoplasmic Potential
Transmembrane570 – 59122Helical; Name=11; Potential
Topological domain592 – 61827Extracellular Potential
Transmembrane619 – 64123Helical; Name=12; Potential
Topological domain642 – 72786Cytoplasmic Potential

Amino acid modifications

Modified residue3771Phosphotyrosine By similarity
Glycosylation1861N-linked (GlcNAc...) Potential
Glycosylation3931N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict2611Y → C in AAC60673. Ref.2
Sequence conflict4991G → S in AAC60673. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P31662 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: C676048C0A6BDF7C

FASTA72781,055
        10         20         30         40         50         60 
MPKNSKVTQR EHSNEHVTES VADLLALEEP VDYKQSVLNV AGETGGKQKV AEEELDAEDR 

        70         80         90        100        110        120 
PAWNSKLQYI LAQIGFSVGL GNIWRFPYLC QKNGGGAYLV PYLVLLIIIG IPLFFLELAV 

       130        140        150        160        170        180 
GQRIRRGSIG VWHYVCPRLG GIGFSSCIVC LFVGLYYNVI IGWSVFYFFK SFQYPLPWSE 

       190        200        210        220        230        240 
CPVIRNGTVA VVEPECEKSS ATTYFWYREA LDISNSISES GGLNWKMTVC LLVAWSIVGM 

       250        260        270        280        290        300 
AVVKGIQSSG KVMYFSSLFP YVVLACFLVR GLLLRGAVDG ILHMFTPKLD KMLDPQVWRE 

       310        320        330        340        350        360 
AATQVFFALG LGFGGVIAFS SYNKQDNNCH FDAALVSFIN FFTSVLATLV VFAVLGFKAN 

       370        380        390        400        410        420 
IMNEKCVVEN AEKILGYLNS NVLSRDLIPP HVNFSHLTTK DYSEMYNVIM TVKEKQFSAL 

       430        440        450        460        470        480 
GLDPCLLEDE LDKSVQGTGL AFIAFTEAMT HFPASPFWSV MFFLMLINLG LGSMIGTMAG 

       490        500        510        520        530        540 
ITTPIIDTFK VPKEMFTVGC CVFAFFVGLL FVQRSGNYFV TMFDDYSATL PLTVIVILEN 

       550        560        570        580        590        600 
IAVAWIYGTK KFMQELTEML GFRPYRFYFY MWKFVSPLCM AVLTTASIIQ LGVSPPGYSA 

       610        620        630        640        650        660 
WIKEEAAERY LYFPNWAMAL LITLIAVATL PIPVVFILRH FHLLSDGSNT LSVSYKKGRM 

       670        680        690        700        710        720 
MKDISNLEEN DETRFILSKV PSEAPSPMPT HRSYLGPGST SPLESSSHPN GRYGSGYLLA 


STPESEL 

« Hide

References

[1]"A rat brain cDNA encoding the neurotransmitter transporter with an unusual structure."
Liu Q.-R., Mandiyan S., Lopez-Corcuera B., Nelson H., Nelson N.
FEBS Lett. 315:114-118(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Characterization of an atypical member of the Na+/Cl(-)-dependent transporter family: chromosomal localization and distribution in GABAergic and glutamatergic neurons in the rat brain."
el Mestikawy S., Giros B., Pohl M., Hamon M., Kingsmore S.F., Seldin M.F., Caron M.G.
J. Neurochem. 62:445-455(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The repertoire of solute carriers of family 6: identification of new human and rodent genes."
Hoglund P.J., Adzic D., Scicluna S.J., Lindblom J., Fredriksson R.
Biochem. Biophys. Res. Commun. 336:175-189(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[4]"The orphan transporter Rxt1/NTT4 (SLC6A17) functions as a synaptic vesicle amino acid transporter selective for proline, glycine, leucine, and alanine."
Parra L.A., Baust T., El Mestikawy S., Quiroz M., Hoffman B., Haflett J.M., Yao J.K., Torres G.E.
Mol. Pharmacol. 74:1521-1532(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[5]"Synaptic vesicle protein NTT4/XT1 (SLC6A17) catalyzes Na+-coupled neutral amino acid transport."
Zaia K.A., Reimer R.J.
J. Biol. Chem. 284:8439-8448(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, PH DEPENDENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L06434 mRNA. Translation: AAB24776.1.
S68944 mRNA. Translation: AAC60673.1.
PIRI56506.
S27043.
RefSeqNP_001028251.1. NM_001033079.1.
UniGeneRn.186904.

3D structure databases

ProteinModelPortalP31662.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid565981. 1 interaction.

Protein family/group databases

TCDB2.A.22.6.2. the neurotransmitter:sodium symporter (nss) family.

PTM databases

PhosphoSiteP31662.

Proteomic databases

PRIDEP31662.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000075653; ENSRNOP00000065179; ENSRNOG00000050090.
GeneID613226.
KEGGrno:613226.

Organism-specific databases

CTD388662.
RGD1587185. Slc6a17.

Phylogenomic databases

GeneTreeENSGT00750000117287.
HOVERGENHBG071421.
KOK05048.
OMAMEMYNVI.
OrthoDBEOG793B71.
PhylomeDBP31662.

Gene expression databases

GenevestigatorP31662.

Family and domain databases

InterProIPR000175. Na/ntran_symport.
IPR002438. Na/ntran_symport_orphan.
[Graphical view]
PANTHERPTHR11616. PTHR11616. 1 hit.
PfamPF00209. SNF. 1 hit.
[Graphical view]
PRINTSPR00176. NANEUSMPORT.
PR01206. ORPHTRNSPORT.
PROSITEPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio714599.
PROP31662.

Entry information

Entry nameS6A17_RAT
AccessionPrimary (citable) accession number: P31662
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families