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Protein

2-methylcitrate synthase

Gene

prpC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the Claisen condensation of propionyl-CoA and oxaloacetate (OAA) to yield (2S,3S)-2-methylcitrate (2-MC) and CoA. Also catalyzes the condensation of oxaloacetate with acetyl-CoA to yield citrate but with a lower specificity.3 Publications

Catalytic activityi

Propanoyl-CoA + H2O + oxaloacetate = (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA.2 Publications
Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.2 Publications

Kineticsi

  1. KM=5 µM for oxaloacetate (at pH 8 and 35 degrees Celsius)1 Publication
  2. KM=17 µM for propionyl-CoA (at pH 7.4)1 Publication
  3. KM=37 µM for propionyl-CoA (at pH 8 and 35 degrees Celsius)1 Publication
  4. KM=101 µM for acetyl-CoA (at pH 8 and 35 degrees Celsius)1 Publication
  1. Vmax=0.11 µmol/min/mg enzyme with acetyl-CoA as substrate (at pH 8 and 35 degrees Celsius)1 Publication
  2. Vmax=0.33 µmol/min/mg enzyme with propionyl-CoA as substrate (at pH 8 and 35 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 9.1 Publication

Temperature dependencei

Optimum temperature is between 45 and 50 degrees Celsius.1 Publication

Pathwayi: propanoate degradation

This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (gltA)
  2. 2-methylcitrate dehydratase (prpD), Aconitate hydratase B (acnB), Aconitate hydratase A (acnA), 2-methylcitrate synthase (prpC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei82SubstrateBy similarity1
Binding sitei200SubstrateBy similarity1
Active sitei235By similarity1
Active sitei274By similarity1
Binding sitei283SubstrateBy similarity1
Active sitei325By similarity1
Binding sitei350SubstrateBy similarity1
Binding sitei369SubstrateBy similarity1

GO - Molecular functioni

  • 2-methylcitrate synthase activity Source: UniProtKB
  • citrate (Si)-synthase activity Source: UniProtKB

GO - Biological processi

  • propionate metabolic process, methylcitrate cycle Source: UniProtKB
  • tricarboxylic acid cycle Source: UniProtKB-UniPathway

Keywordsi

Molecular functionTransferase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

BioCyciEcoCyc:G6198-MONOMER
MetaCyc:G6198-MONOMER
BRENDAi2.3.3.5 2026
UniPathwayiUPA00223; UER00718
UPA00946

Names & Taxonomyi

Protein namesi
Recommended name:
2-methylcitrate synthase1 Publication (EC:2.3.3.52 Publications)
Short name:
2-MCS1 Publication
Short name:
MCS1 Publication
Alternative name(s):
(2S,3S)-2-methylcitrate synthase1 Publication
Citrate synthase1 Publication (EC:2.3.3.162 Publications)
Gene namesi
Name:prpC1 Publication
Synonyms:yahS, yzzD
Ordered Locus Names:b0333, JW0324
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11756 prpC

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001699811 – 3892-methylcitrate synthaseAdd BLAST389

Proteomic databases

PaxDbiP31660
PRIDEiP31660

Expressioni

Inductioni

By propionate, but not acetate or glucose. Expression of prpBCDE operon is regulated by PrpR, CRP and a variety of sugars such as arabinose, galactose, glucose mannose and xylose.5 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4259809, 8 interactors
DIPiDIP-10579N
IntActiP31660, 2 interactors
STRINGi316407.85674475

Structurei

3D structure databases

ProteinModelPortaliP31660
SMRiP31660
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni268 – 272Coenzyme A bindingBy similarity5

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105BZN Bacteria
COG0372 LUCA
HOGENOMiHOG000021225
InParanoidiP31660
KOiK01659
OMAiLNKADYW
PhylomeDBiP31660

Family and domain databases

Gene3Di1.10.230.10, 1 hit
1.10.580.10, 2 hits
InterProiView protein in InterPro
IPR011278 2-MeCitrate/Citrate_synth_II
IPR016142 Citrate_synth-like_lrg_a-sub
IPR016143 Citrate_synth-like_sm_a-sub
IPR002020 Citrate_synthase
IPR019810 Citrate_synthase_AS
IPR024176 Citrate_synthase_bac-typ
IPR036969 Citrate_synthase_sf
PANTHERiPTHR11739 PTHR11739, 1 hit
PfamiView protein in Pfam
PF00285 Citrate_synt, 1 hit
PIRSFiPIRSF001369 Citrate_synth, 1 hit
PRINTSiPR00143 CITRTSNTHASE
SUPFAMiSSF48256 SSF48256, 1 hit
TIGRFAMsiTIGR01800 cit_synth_II, 1 hit
PROSITEiView protein in PROSITE
PS00480 CITRATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P31660-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDTTILQNS THVIKPKKSV ALSGVPAGNT ALCTVGKSGN DLHYRGYDIL
60 70 80 90 100
DLAKHCEFEE VAHLLIHGKL PTRDELAAYK TKLKALRGLP ANVRTVLEAL
110 120 130 140 150
PAASHPMDVM RTGVSALGCT LPEKEGHTVS GARDIADKLL ASLSSILLYW
160 170 180 190 200
YHYSHNGERI QPETDDDSIG GHFLHLLHGE KPSQSWEKAM HISLVLYAEH
210 220 230 240 250
EFNASTFTSR VIAGTGSDMY SAIIGAIGAL RGPKHGGANE VSLEIQQRYE
260 270 280 290 300
TPDEAEADIR KRVENKEVVI GFGHPVYTIA DPRHQVIKRV AKQLSQEGGS
310 320 330 340 350
LKMYNIADRL ETVMWESKKM FPNLDWFSAV SYNMMGVPTE MFTPLFVIAR
360 370 380
VTGWAAHIIE QRQDNKIIRP SANYVGPEDR PFVALDKRQ
Length:389
Mass (Da):43,102
Last modified:November 1, 1997 - v2
Checksum:i02B779E7AD4581C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73857 Genomic DNA Translation: AAB18057.1
U00096 Genomic DNA Translation: AAC73436.1
AP009048 Genomic DNA Translation: BAE76115.1
PIRiE64760
RefSeqiNP_414867.1, NC_000913.3
WP_001285927.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73436; AAC73436; b0333
BAE76115; BAE76115; BAE76115
GeneIDi947528
KEGGiecj:JW0324
eco:b0333
PATRICifig|1411691.4.peg.1944

Similar proteinsi

Entry informationi

Entry nameiPRPC_ECOLI
AccessioniPrimary (citable) accession number: P31660
Secondary accession number(s): P77217, Q2MC91
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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