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Protein

Sodium- and chloride-dependent GABA transporter 2

Gene

Slc6a13

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-dependent GABA and taurine transporter. In presynaptic terminals, regulates GABA signaling termination through GABA uptake. In the liver, may be the major contributor for GABA uptake. Also involved in beta-alanine transport.1 Publication

Kineticsi

  1. KM=18 µM for GABA
  2. KM=28 µM for beta-alanine
  3. KM=540 µM for taurine

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Biological processi

    Neurotransmitter transport, Symport, Transport

    Enzyme and pathway databases

    ReactomeiR-MMU-442660. Na+/Cl- dependent neurotransmitter transporters.
    R-MMU-888593. Reuptake of GABA.

    Protein family/group databases

    TCDBi2.A.22.3.8. the neurotransmitter:sodium symporter (nss) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium- and chloride-dependent GABA transporter 2
    Short name:
    GAT-2
    Alternative name(s):
    Sodium- and chloride-dependent GABA transporter 3
    Short name:
    GAT-3
    Solute carrier family 6 member 13
    Gene namesi
    Name:Slc6a13
    Synonyms:Gabt2, Gabt3, Gat-3, Gat2, Gat3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 6

    Organism-specific databases

    MGIiMGI:95629. Slc6a13.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4040CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei41 – 6121Helical; Name=1Sequence analysisAdd
    BLAST
    Transmembranei68 – 8821Helical; Name=2Sequence analysisAdd
    BLAST
    Transmembranei121 – 14121Helical; Name=3Sequence analysisAdd
    BLAST
    Topological domaini142 – 20665ExtracellularSequence analysisAdd
    BLAST
    Transmembranei207 – 22721Helical; Name=4Sequence analysisAdd
    BLAST
    Transmembranei233 – 25321Helical; Name=5Sequence analysisAdd
    BLAST
    Transmembranei282 – 30221Helical; Name=6Sequence analysisAdd
    BLAST
    Transmembranei319 – 33921Helical; Name=7Sequence analysisAdd
    BLAST
    Transmembranei366 – 38621Helical; Name=8Sequence analysisAdd
    BLAST
    Transmembranei418 – 43821Helical; Name=9Sequence analysisAdd
    BLAST
    Transmembranei453 – 47321Helical; Name=10Sequence analysisAdd
    BLAST
    Transmembranei490 – 51021Helical; Name=11Sequence analysisAdd
    BLAST
    Transmembranei528 – 54821Helical; Name=12Sequence analysisAdd
    BLAST
    Topological domaini549 – 60254CytoplasmicSequence analysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mutant animals are born at the expected Mendelian ratio. They appear normal, fertile, with a normal life span. Tissue taurine levels are altered, with 50% decrease in the liver and 20% increase in the brain.1 Publication

    Chemistry

    ChEMBLiCHEMBL2096685.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 602602Sodium- and chloride-dependent GABA transporter 2PRO_0000214793Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi153 ↔ 162By similarity
    Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence analysis
    Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence analysis
    Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence analysis
    Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence analysis
    Modified residuei587 – 5871PhosphothreonineCombined sources
    Modified residuei591 – 5911PhosphoserineCombined sources

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP31649.
    PaxDbiP31649.
    PeptideAtlasiP31649.
    PRIDEiP31649.

    PTM databases

    iPTMnetiP31649.
    PhosphoSiteiP31649.

    Expressioni

    Tissue specificityi

    Expressed at high levels in liver, followed by kidney and leptomeninges, and very low levels in the cerebellum (at protein level).In the brain, detected in some blood vessels (at protein level). In the kidney, expressed in the cortex, including parts of the proximal tubules, but not in the medulla (at protein level). In the liver, highest expression in periportal hepatocytes, with highest density at the vascular side (at protein level). Also detected at low levels in other organs, including skeletal muscle.1 Publication

    Developmental stagei

    Abundant in neonatal brain, but not in adult brain.

    Gene expression databases

    BgeeiP31649.
    ExpressionAtlasiP31649. baseline and differential.
    GenevisibleiP31649. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000066779.

    Chemistry

    BindingDBiP31649.

    Structurei

    3D structure databases

    ProteinModelPortaliP31649.
    SMRiP31649. Positions 31-573.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG3659. Eukaryota.
    COG0733. LUCA.
    GeneTreeiENSGT00760000118857.
    HOGENOMiHOG000116406.
    HOVERGENiHBG071421.
    InParanoidiP31649.
    KOiK05039.
    OMAiGGCHHEW.
    OrthoDBiEOG793B71.
    PhylomeDBiP31649.
    TreeFamiTF343812.

    Family and domain databases

    InterProiIPR000175. Na/ntran_symport.
    IPR002981. Na/ntran_symport_GABA_GAT2.
    [Graphical view]
    PANTHERiPTHR11616. PTHR11616. 1 hit.
    PfamiPF00209. SNF. 1 hit.
    [Graphical view]
    PRINTSiPR01196. GAT2TRNSPORT.
    PR00176. NANEUSMPORT.
    PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31649-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MENRASGTTS NGETKPVCPA MEKVEEDGTL EREHWNNKME FVLSVAGEII
    60 70 80 90 100
    GLGNVWRFPY LCYKNGGGAF FIPYLIFLFT CGIPVFFLET ALGQYTNQGG
    110 120 130 140 150
    ITAWRRICPI FEGIGYASQM IVSLLNVYYI VVLAWALFYL FSSFTTDLPW
    160 170 180 190 200
    GSCSHEWNTE NCVEFQKAND SMNVTSENAT SPVIEFWERR VLKLSDGIQH
    210 220 230 240 250
    LGSLRWELVL CLLLAWIICY FCIWKGVKST GKVVYFTATF PYLMLVVLLI
    260 270 280 290 300
    RGVTLPGAAQ GIQFYLYPNI TRLWDPQVWM DAGTQIFFSF AICLGCLTAL
    310 320 330 340 350
    GSYNKYHNNC YRDCIALCIL NSSTSFMAGF AIFSILGFMS QEQGVPISEV
    360 370 380 390 400
    AESGPGLAFI AYPRAVVMLP FSPLWACCFF FMVVLLGLDS QFVCVESLVT
    410 420 430 440 450
    ALVDMYPRVF RKKNRREVLI LIVSVISFFI GLIMLTEGGM YVFQLFDYYA
    460 470 480 490 500
    ASGMCLLFVA IFESLCVAWV YGAGRFYDNI EDMIGYKPWP LIKYCWLFFT
    510 520 530 540 550
    PAVCLATFLF SLIKYTPLTY NKKYTYPWWG DALGWLLALS SMICIPAWSI
    560 570 580 590 600
    YKLRTLKGPL RERLRQLVCP AEDLPQKNQP EPTAPATPMT SLLRLTELES

    NC
    Length:602
    Mass (Da):68,285
    Last modified:July 1, 1993 - v1
    Checksum:i372FE77F3126ABEA
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L04663 mRNA. No translation available.
    BC023117 mRNA. Translation: AAH23117.1.
    BC029637 mRNA. Translation: AAH29637.1.
    CCDSiCCDS20490.1.
    PIRiA44409.
    RefSeqiNP_653095.1. NM_144512.2.
    UniGeneiMm.258596.

    Genome annotation databases

    EnsembliENSMUST00000064580; ENSMUSP00000066779; ENSMUSG00000030108.
    GeneIDi14412.
    KEGGimmu:14412.
    UCSCiuc009doi.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L04663 mRNA. No translation available.
    BC023117 mRNA. Translation: AAH23117.1.
    BC029637 mRNA. Translation: AAH29637.1.
    CCDSiCCDS20490.1.
    PIRiA44409.
    RefSeqiNP_653095.1. NM_144512.2.
    UniGeneiMm.258596.

    3D structure databases

    ProteinModelPortaliP31649.
    SMRiP31649. Positions 31-573.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000066779.

    Chemistry

    BindingDBiP31649.
    ChEMBLiCHEMBL2096685.

    Protein family/group databases

    TCDBi2.A.22.3.8. the neurotransmitter:sodium symporter (nss) family.

    PTM databases

    iPTMnetiP31649.
    PhosphoSiteiP31649.

    Proteomic databases

    MaxQBiP31649.
    PaxDbiP31649.
    PeptideAtlasiP31649.
    PRIDEiP31649.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000064580; ENSMUSP00000066779; ENSMUSG00000030108.
    GeneIDi14412.
    KEGGimmu:14412.
    UCSCiuc009doi.1. mouse.

    Organism-specific databases

    CTDi6540.
    MGIiMGI:95629. Slc6a13.

    Phylogenomic databases

    eggNOGiKOG3659. Eukaryota.
    COG0733. LUCA.
    GeneTreeiENSGT00760000118857.
    HOGENOMiHOG000116406.
    HOVERGENiHBG071421.
    InParanoidiP31649.
    KOiK05039.
    OMAiGGCHHEW.
    OrthoDBiEOG793B71.
    PhylomeDBiP31649.
    TreeFamiTF343812.

    Enzyme and pathway databases

    ReactomeiR-MMU-442660. Na+/Cl- dependent neurotransmitter transporters.
    R-MMU-888593. Reuptake of GABA.

    Miscellaneous databases

    PROiP31649.
    SOURCEiSearch...

    Gene expression databases

    BgeeiP31649.
    ExpressionAtlasiP31649. baseline and differential.
    GenevisibleiP31649. MM.

    Family and domain databases

    InterProiIPR000175. Na/ntran_symport.
    IPR002981. Na/ntran_symport_GABA_GAT2.
    [Graphical view]
    PANTHERiPTHR11616. PTHR11616. 1 hit.
    PfamiPF00209. SNF. 1 hit.
    [Graphical view]
    PRINTSiPR01196. GAT2TRNSPORT.
    PR00176. NANEUSMPORT.
    PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Molecular characterization of four pharmacologically distinct gamma-aminobutyric acid transporters in mouse brain."
      Liu Q.-R., Lopez-Coecuera B., Mandiyan S., Nelson H., Nelson N.
      J. Biol. Chem. 268:2106-2112(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney and Liver.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-587, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-587 AND SER-591, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Kidney and Liver.
    5. "Deletion of the gamma-aminobutyric acid transporter 2 (GAT2 and SLC6A13) gene in mice leads to changes in liver and brain taurine contents."
      Zhou Y., Holmseth S., Guo C., Hassel B., Hofner G., Huitfeldt H.S., Wanner K.T., Danbolt N.C.
      J. Biol. Chem. 287:35733-35746(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiS6A13_MOUSE
    AccessioniPrimary (citable) accession number: P31649
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: July 6, 2016
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.