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P31649 (S6A13_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium- and chloride-dependent GABA transporter 2
Short name=GAT-2
Alternative name(s):
Sodium- and chloride-dependent GABA transporter 3
Short name=GAT-3
Solute carrier family 6 member 13
Gene names
Name:Slc6a13
Synonyms:Gabt2, Gabt3, Gat-3, Gat2, Gat3
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. Can also transport beta-alanine and taurine.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Brain, liver and kidney.

Developmental stage

Abundant in neonatal brain but not in adult brain.

Sequence similarities

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A13 subfamily. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=18 µM for GABA

KM=28 µM for beta-alanine

KM=540 µM for taurine

Ontologies

Keywords
   Biological processNeurotransmitter transport
Symport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentintegral to plasma membrane

Inferred from electronic annotation. Source: InterPro

   Molecular functionneurotransmitter:sodium symporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 602602Sodium- and chloride-dependent GABA transporter 2
PRO_0000214793

Regions

Topological domain1 – 4040Cytoplasmic Potential
Transmembrane41 – 6121Helical; Name=1; Potential
Transmembrane68 – 8821Helical; Name=2; Potential
Transmembrane121 – 14121Helical; Name=3; Potential
Topological domain142 – 20665Extracellular Potential
Transmembrane207 – 22721Helical; Name=4; Potential
Transmembrane233 – 25321Helical; Name=5; Potential
Transmembrane282 – 30221Helical; Name=6; Potential
Transmembrane319 – 33921Helical; Name=7; Potential
Transmembrane366 – 38621Helical; Name=8; Potential
Transmembrane418 – 43821Helical; Name=9; Potential
Transmembrane453 – 47321Helical; Name=10; Potential
Transmembrane490 – 51021Helical; Name=11; Potential
Transmembrane528 – 54821Helical; Name=12; Potential
Topological domain549 – 60254Cytoplasmic Potential

Amino acid modifications

Modified residue5871Phosphothreonine Ref.3
Modified residue5911Phosphoserine Ref.3
Glycosylation1691N-linked (GlcNAc...) Potential
Glycosylation1731N-linked (GlcNAc...) Potential
Glycosylation1781N-linked (GlcNAc...) Potential
Glycosylation2691N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P31649 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 372FE77F3126ABEA

FASTA60268,285
        10         20         30         40         50         60 
MENRASGTTS NGETKPVCPA MEKVEEDGTL EREHWNNKME FVLSVAGEII GLGNVWRFPY 

        70         80         90        100        110        120 
LCYKNGGGAF FIPYLIFLFT CGIPVFFLET ALGQYTNQGG ITAWRRICPI FEGIGYASQM 

       130        140        150        160        170        180 
IVSLLNVYYI VVLAWALFYL FSSFTTDLPW GSCSHEWNTE NCVEFQKAND SMNVTSENAT 

       190        200        210        220        230        240 
SPVIEFWERR VLKLSDGIQH LGSLRWELVL CLLLAWIICY FCIWKGVKST GKVVYFTATF 

       250        260        270        280        290        300 
PYLMLVVLLI RGVTLPGAAQ GIQFYLYPNI TRLWDPQVWM DAGTQIFFSF AICLGCLTAL 

       310        320        330        340        350        360 
GSYNKYHNNC YRDCIALCIL NSSTSFMAGF AIFSILGFMS QEQGVPISEV AESGPGLAFI 

       370        380        390        400        410        420 
AYPRAVVMLP FSPLWACCFF FMVVLLGLDS QFVCVESLVT ALVDMYPRVF RKKNRREVLI 

       430        440        450        460        470        480 
LIVSVISFFI GLIMLTEGGM YVFQLFDYYA ASGMCLLFVA IFESLCVAWV YGAGRFYDNI 

       490        500        510        520        530        540 
EDMIGYKPWP LIKYCWLFFT PAVCLATFLF SLIKYTPLTY NKKYTYPWWG DALGWLLALS 

       550        560        570        580        590        600 
SMICIPAWSI YKLRTLKGPL RERLRQLVCP AEDLPQKNQP EPTAPATPMT SLLRLTELES 


NC

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of four pharmacologically distinct gamma-aminobutyric acid transporters in mouse brain."
Liu Q.-R., Lopez-Coecuera B., Mandiyan S., Nelson H., Nelson N.
J. Biol. Chem. 268:2106-2112(1993) [PubMed: 8420981] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney and Liver.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-587 AND SER-591, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L04663 mRNA. No translation available.
BC023117 mRNA. Translation: AAH23117.1.
BC029637 mRNA. Translation: AAH29637.1.
IPIIPI00136863.
PIRA44409.
RefSeqNP_653095.1. NM_144512.2.
UniGeneMm.258596.

3D structure databases

ProteinModelPortalP31649.
ModBaseSearch...

Protein family/group databases

TCDB2.A.22.3.8. neurotransmitter:sodium symporter (NSS) family.

PTM databases

PhosphoSiteP31649.

Proteomic databases

PRIDEP31649.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000064580; ENSMUSP00000066779; ENSMUSG00000030108.
GeneID14412.
KEGGmmu:14412.
UCSCuc009doi.1. mouse.

Organism-specific databases

CTD6540.
MGIMGI:95629. Slc6a13.

Phylogenomic databases

eggNOGroNOG05228.
HOGENOMHBG702834.
HOVERGENHBG071421.
InParanoidP31649.
OMAYASQMIV.
OrthoDBEOG43N7CD.
PhylomeDBP31649.

Gene expression databases

ArrayExpressP31649.
BgeeP31649.
GenevestigatorP31649.
GermOnlineENSMUSG00000030108. Mus musculus.

Family and domain databases

InterProIPR000175. Na/ntran_symport.
IPR002981. Na/ntran_symport_GABA_GAT2.
[Graphical view]
KOK05046.
PANTHERPTHR11616. Na/ntran_symport. 1 hit.
PfamPF00209. SNF. 1 hit.
[Graphical view]
PRINTSPR01196. GAT2TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio285993.
SOURCESearch...

Entry information

Entry nameS6A13_MOUSE
AccessionPrimary (citable) accession number: P31649
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 16, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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