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Protein

Sodium- and chloride-dependent GABA transporter 2

Gene

Slc6a13

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sodium-dependent GABA and taurine transporter. In presynaptic terminals, regulates GABA signaling termination through GABA uptake. In the liver, may be the major contributor for GABA uptake. Also involved in beta-alanine transport.1 Publication

Kineticsi

  1. KM=18 µM for GABA
  2. KM=28 µM for beta-alanine
  3. KM=540 µM for taurine

    GO - Molecular functioni

    Keywordsi

    Biological processNeurotransmitter transport, Symport, Transport

    Enzyme and pathway databases

    ReactomeiR-MMU-442660 Na+/Cl- dependent neurotransmitter transporters
    R-MMU-888593 Reuptake of GABA

    Protein family/group databases

    TCDBi2.A.22.3.8 the neurotransmitter:sodium symporter (nss) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium- and chloride-dependent GABA transporter 2
    Short name:
    GAT-2
    Alternative name(s):
    Sodium- and chloride-dependent GABA transporter 3
    Short name:
    GAT-3
    Solute carrier family 6 member 13
    Gene namesi
    Name:Slc6a13
    Synonyms:Gabt2, Gabt3, Gat-3, Gat2, Gat3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 6

    Organism-specific databases

    MGIiMGI:95629 Slc6a13

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 40CytoplasmicSequence analysisAdd BLAST40
    Transmembranei41 – 61Helical; Name=1Sequence analysisAdd BLAST21
    Transmembranei68 – 88Helical; Name=2Sequence analysisAdd BLAST21
    Transmembranei121 – 141Helical; Name=3Sequence analysisAdd BLAST21
    Topological domaini142 – 206ExtracellularSequence analysisAdd BLAST65
    Transmembranei207 – 227Helical; Name=4Sequence analysisAdd BLAST21
    Transmembranei233 – 253Helical; Name=5Sequence analysisAdd BLAST21
    Transmembranei282 – 302Helical; Name=6Sequence analysisAdd BLAST21
    Transmembranei319 – 339Helical; Name=7Sequence analysisAdd BLAST21
    Transmembranei366 – 386Helical; Name=8Sequence analysisAdd BLAST21
    Transmembranei418 – 438Helical; Name=9Sequence analysisAdd BLAST21
    Transmembranei453 – 473Helical; Name=10Sequence analysisAdd BLAST21
    Transmembranei490 – 510Helical; Name=11Sequence analysisAdd BLAST21
    Transmembranei528 – 548Helical; Name=12Sequence analysisAdd BLAST21
    Topological domaini549 – 602CytoplasmicSequence analysisAdd BLAST54

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mutant animals are born at the expected Mendelian ratio. They appear normal, fertile, with a normal life span. Tissue taurine levels are altered, with 50% decrease in the liver and 20% increase in the brain.1 Publication

    Chemistry databases

    ChEMBLiCHEMBL5205

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002147931 – 602Sodium- and chloride-dependent GABA transporter 2Add BLAST602

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi153 ↔ 162By similarity
    Glycosylationi169N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi173N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi178N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi269N-linked (GlcNAc...) asparagineSequence analysis1
    Modified residuei587PhosphothreonineCombined sources1
    Modified residuei591PhosphoserineCombined sources1

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP31649
    PaxDbiP31649
    PeptideAtlasiP31649
    PRIDEiP31649

    PTM databases

    iPTMnetiP31649
    PhosphoSitePlusiP31649

    Expressioni

    Tissue specificityi

    Expressed at high levels in liver, followed by kidney and leptomeninges, and very low levels in the cerebellum (at protein level).In the brain, detected in some blood vessels (at protein level). In the kidney, expressed in the cortex, including parts of the proximal tubules, but not in the medulla (at protein level). In the liver, highest expression in periportal hepatocytes, with highest density at the vascular side (at protein level). Also detected at low levels in other organs, including skeletal muscle.1 Publication

    Developmental stagei

    Abundant in neonatal brain, but not in adult brain.

    Gene expression databases

    BgeeiENSMUSG00000030108
    ExpressionAtlasiP31649 baseline and differential
    GenevisibleiP31649 MM

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000066779

    Chemistry databases

    BindingDBiP31649

    Structurei

    3D structure databases

    ProteinModelPortaliP31649
    SMRiP31649
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG3659 Eukaryota
    COG0733 LUCA
    GeneTreeiENSGT00760000118857
    HOGENOMiHOG000116406
    HOVERGENiHBG071421
    InParanoidiP31649
    KOiK05039
    OMAiFQRTNGS
    OrthoDBiEOG091G08PX
    PhylomeDBiP31649
    TreeFamiTF343812

    Family and domain databases

    InterProiView protein in InterPro
    IPR000175 Na/ntran_symport
    IPR002981 Na/ntran_symport_GABA_GAT2
    IPR037272 SNS_sf
    PANTHERiPTHR11616 PTHR11616, 1 hit
    PfamiView protein in Pfam
    PF00209 SNF, 1 hit
    PRINTSiPR01196 GAT2TRNSPORT
    PR00176 NANEUSMPORT
    SUPFAMiSSF161070 SSF161070, 1 hit
    PROSITEiView protein in PROSITE
    PS00610 NA_NEUROTRAN_SYMP_1, 1 hit
    PS00754 NA_NEUROTRAN_SYMP_2, 1 hit
    PS50267 NA_NEUROTRAN_SYMP_3, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P31649-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MENRASGTTS NGETKPVCPA MEKVEEDGTL EREHWNNKME FVLSVAGEII
    60 70 80 90 100
    GLGNVWRFPY LCYKNGGGAF FIPYLIFLFT CGIPVFFLET ALGQYTNQGG
    110 120 130 140 150
    ITAWRRICPI FEGIGYASQM IVSLLNVYYI VVLAWALFYL FSSFTTDLPW
    160 170 180 190 200
    GSCSHEWNTE NCVEFQKAND SMNVTSENAT SPVIEFWERR VLKLSDGIQH
    210 220 230 240 250
    LGSLRWELVL CLLLAWIICY FCIWKGVKST GKVVYFTATF PYLMLVVLLI
    260 270 280 290 300
    RGVTLPGAAQ GIQFYLYPNI TRLWDPQVWM DAGTQIFFSF AICLGCLTAL
    310 320 330 340 350
    GSYNKYHNNC YRDCIALCIL NSSTSFMAGF AIFSILGFMS QEQGVPISEV
    360 370 380 390 400
    AESGPGLAFI AYPRAVVMLP FSPLWACCFF FMVVLLGLDS QFVCVESLVT
    410 420 430 440 450
    ALVDMYPRVF RKKNRREVLI LIVSVISFFI GLIMLTEGGM YVFQLFDYYA
    460 470 480 490 500
    ASGMCLLFVA IFESLCVAWV YGAGRFYDNI EDMIGYKPWP LIKYCWLFFT
    510 520 530 540 550
    PAVCLATFLF SLIKYTPLTY NKKYTYPWWG DALGWLLALS SMICIPAWSI
    560 570 580 590 600
    YKLRTLKGPL RERLRQLVCP AEDLPQKNQP EPTAPATPMT SLLRLTELES

    NC
    Length:602
    Mass (Da):68,285
    Last modified:July 1, 1993 - v1
    Checksum:i372FE77F3126ABEA
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L04663 mRNA No translation available.
    BC023117 mRNA Translation: AAH23117.1
    BC029637 mRNA Translation: AAH29637.1
    CCDSiCCDS20490.1
    PIRiA44409
    RefSeqiNP_653095.1, NM_144512.2
    UniGeneiMm.258596

    Genome annotation databases

    EnsembliENSMUST00000064580; ENSMUSP00000066779; ENSMUSG00000030108
    GeneIDi14412
    KEGGimmu:14412
    UCSCiuc009doi.1 mouse

    Similar proteinsi

    Entry informationi

    Entry nameiS6A13_MOUSE
    AccessioniPrimary (citable) accession number: P31649
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: March 28, 2018
    This is version 135 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health