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Protein

Sodium- and chloride-dependent GABA transporter 1

Gene

Slc6a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines or cocaine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi59Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi61Sodium 2; via carbonyl oxygenBy similarity1
Metal bindingi62Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi66Sodium 2By similarity1
Metal bindingi295Sodium 2By similarity1
Metal bindingi327Sodium 2By similarity1
Metal bindingi392Sodium 1; via carbonyl oxygenBy similarity1
Metal bindingi395Sodium 1By similarity1
Metal bindingi396Sodium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processNeurotransmitter transport, Symport, Transport
LigandMetal-binding, Sodium

Enzyme and pathway databases

ReactomeiR-MMU-442660 Na+/Cl- dependent neurotransmitter transporters
R-MMU-888593 Reuptake of GABA

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent GABA transporter 1
Short name:
GAT-1
Alternative name(s):
Solute carrier family 6 member 1
Gene namesi
Name:Slc6a1
Synonyms:Gabt1, Gat-1, Gat1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:95627 Slc6a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 52CytoplasmicSequence analysisAdd BLAST52
Transmembranei53 – 73Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei81 – 100Helical; Name=2Sequence analysisAdd BLAST20
Transmembranei124 – 144Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini145 – 211ExtracellularSequence analysisAdd BLAST67
Transmembranei212 – 230Helical; Name=4Sequence analysisAdd BLAST19
Transmembranei239 – 256Helical; Name=5Sequence analysisAdd BLAST18
Transmembranei292 – 309Helical; Name=6Sequence analysisAdd BLAST18
Transmembranei321 – 342Helical; Name=7Sequence analysisAdd BLAST22
Transmembranei375 – 394Helical; Name=8Sequence analysisAdd BLAST20
Transmembranei422 – 440Helical; Name=9Sequence analysisAdd BLAST19
Transmembranei457 – 477Helical; Name=10Sequence analysisAdd BLAST21
Transmembranei498 – 517Helical; Name=11Sequence analysisAdd BLAST20
Transmembranei536 – 554Helical; Name=12Sequence analysisAdd BLAST19
Topological domaini555 – 599CytoplasmicSequence analysisAdd BLAST45

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5445
GuidetoPHARMACOLOGYi929

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002147441 – 599Sodium- and chloride-dependent GABA transporter 1Add BLAST599

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineCombined sources1
Disulfide bondi164 ↔ 173By similarity
Glycosylationi176N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi181N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi184N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei591PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP31648
PaxDbiP31648
PeptideAtlasiP31648
PRIDEiP31648

PTM databases

iPTMnetiP31648
PhosphoSitePlusiP31648

Expressioni

Tissue specificityi

Brain. Expressed in the dentate gyrus of hippocampus, striatum and cerebellum (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000030310
ExpressionAtlasiP31648 baseline and differential
GenevisibleiP31648 MM

Interactioni

Subunit structurei

Interacts with MPP5.1 Publication

Protein-protein interaction databases

BioGridi2312392 interactors.
STRINGi10090.ENSMUSP00000032454

Chemistry databases

BindingDBiP31648

Structurei

3D structure databases

ProteinModelPortaliP31648
SMRiP31648
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi597 – 599PDZ-binding3

Domaini

The PDZ domain-binding motif is involved in the interaction with MPP5.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3659 Eukaryota
COG0733 LUCA
GeneTreeiENSGT00760000118857
HOGENOMiHOG000116406
HOVERGENiHBG071421
InParanoidiP31648
KOiK05034
OMAiESEVWID
OrthoDBiEOG091G08PX
PhylomeDBiP31648
TreeFamiTF343812

Family and domain databases

CDDicd11506 SLC6sbd_GAT1, 1 hit
InterProiView protein in InterPro
IPR000175 Na/ntran_symport
IPR002980 Na/ntran_symport_GABA_GAT1
IPR037272 SNS_sf
PANTHERiPTHR11616 PTHR11616, 1 hit
PfamiView protein in Pfam
PF00209 SNF, 1 hit
PRINTSiPR01195 GAT1TRNSPORT
PR00176 NANEUSMPORT
SUPFAMiSSF161070 SSF161070, 1 hit
PROSITEiView protein in PROSITE
PS00610 NA_NEUROTRAN_SYMP_1, 1 hit
PS00754 NA_NEUROTRAN_SYMP_2, 1 hit
PS50267 NA_NEUROTRAN_SYMP_3, 1 hit

Sequencei

Sequence statusi: Complete.

P31648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATDNSKVAD GQISTEVSEA PVASDKPKTL VVKVQKKAGD LPDRDTWKGR
60 70 80 90 100
FDFLMSCVGY AIGLGNVWRF PYLCGKNGGG AFLIPYFLTL IFAGVPLFLL
110 120 130 140 150
ECSLGQYTSI GGLGVWKLAP MFKGVGLAAA VLSFWLNIYY IVIISWAIYY
160 170 180 190 200
LYNSFTTTLP WKQCDNPWNT DRCFSNYSLV NTTNMTSAVV EFWERNMHQM
210 220 230 240 250
TDGLDKPGQI RWPLAITLAI AWVLVYFCIW KGVGWTGKVV YFSATYPYIM
260 270 280 290 300
LIILFFRGVT LPGAKEGILF YITPNFRKLS DSEVWLDAAT QIFFSYGLGL
310 320 330 340 350
GSLIALGSYN SFHNNVYRDS IIVCCINSCT SMFAGFVIFS IVGFMAHVTK
360 370 380 390 400
RSIADVAASG PGLAFLAYPE AVTQLPISPL WAILFFSMLL MLGIDSQFCT
410 420 430 440 450
VEGFITALVD EYPRLLRNRR ELFIAAVCIV SYLIGLSNIT QGGIYVFKLF
460 470 480 490 500
DYYSASGMSL LFLVFFECVS ISWFYGVNRF YDNIQEMVGS RPCIWWKLCW
510 520 530 540 550
SFFTPIIVAG VFLFSAVQMT PLTMGSYVFP KWGQGVGWLM ALSSMVLIPG
560 570 580 590
YMAYMFLTLK GSLKQRLQVM IQPSEDIVRP ENGPEQPQAG SSASKEAYI
Length:599
Mass (Da):67,001
Last modified:May 10, 2004 - v2
Checksum:i4FEF85092DC1D045
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti117 – 118KL → NW in M92378 (PubMed:1631167).Curated2
Sequence conflicti126 – 127GL → AV in M92378 (PubMed:1631167).Curated2
Sequence conflicti212 – 213WP → C in M92378 (PubMed:1631167).Curated2
Sequence conflicti285 – 286WL → IF in M92378 (PubMed:1631167).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92377 Genomic DNA No translation available.
M92378 mRNA No translation available.
AK042956 mRNA Translation: BAC31418.1
AK052971 mRNA Translation: BAC35226.1
AK053883 mRNA Translation: BAC35574.1
CCDSiCCDS20434.1
PIRiF46027
RefSeqiNP_848818.1, NM_178703.4
UniGeneiMm.5260

Genome annotation databases

EnsembliENSMUST00000032454; ENSMUSP00000032454; ENSMUSG00000030310
GeneIDi232333
KEGGimmu:232333
UCSCiuc009dhu.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSC6A1_MOUSE
AccessioniPrimary (citable) accession number: P31648
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 10, 2004
Last modified: February 28, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome