P31648 (SC6A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium- and chloride-dependent GABA transporter 1
Short name=GAT-1

Alternative name(s):
Solute carrier family 6 member 1

Gene names

Name:	Slc6a1
Synonyms:	Gabt1, Gat-1, Gat1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
Subunit structure	Interacts with MPP5. Ref.3
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Membrane; Multi-pass membrane protein By similarity. Note: Localized at the plasma membrane and in a subset of intracellular vesicles By similarity. Localized at the presynaptic terminals of interneurons. Ref.3
Tissue specificity	Brain. Expressed in the dentate gyrus of hippocampus, striatum and cerebellum (at protein level). Ref.3
Domain	The PDZ domain-binding motif is involved in the interaction with MPP5 By similarity.
Miscellaneous	This protein is the target of psychomotor stimulants such as amphetamines or cocaine.
Sequence similarities	Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily. [View classification]

Ontologies

Keywords
Biological process	Neurotransmitter transport Symport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gamma-aminobutyric acid import Inferred from electronic annotation. Source: Compara learning Inferred from electronic annotation. Source: Compara negative regulation of synaptic transmission, GABAergic Inferred from electronic annotation. Source: Compara positive regulation of gamma-aminobutyric acid secretion Inferred from electronic annotation. Source: Compara protein homooligomerization Inferred from electronic annotation. Source: Compara response to calcium ion Inferred from electronic annotation. Source: Compara response to cocaine Inferred from electronic annotation. Source: Compara response to estradiol stimulus Inferred from electronic annotation. Source: Compara response to lead ion Inferred from electronic annotation. Source: Compara response to purine-containing compound Inferred from electronic annotation. Source: Compara response to sucrose stimulus Inferred from electronic annotation. Source: Compara response to toxin Inferred from electronic annotation. Source: Compara
Cellular_component	axon Inferred from direct assay PubMed 15479642. Source: MGI cell surface Inferred from electronic annotation. Source: Compara integral to plasma membrane Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from direct assay PubMed 17724084. Source: MGI
Molecular_function	gamma-aminobutyric acid:sodium symporter activity Inferred from electronic annotation. Source: Compara neurotransmitter:sodium symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 599	599	Sodium- and chloride-dependent GABA transporter 1		PRO_0000214744
Regions
	Topological domain	1 – 52	52	Cytoplasmic Potential
	Transmembrane	53 – 73	21	Helical; Name=1; Potential
	Transmembrane	81 – 100	20	Helical; Name=2; Potential
	Transmembrane	124 – 144	21	Helical; Name=3; Potential
	Topological domain	145 – 211	67	Extracellular Potential
	Transmembrane	212 – 230	19	Helical; Name=4; Potential
	Transmembrane	239 – 256	18	Helical; Name=5; Potential
	Transmembrane	292 – 309	18	Helical; Name=6; Potential
	Transmembrane	321 – 342	22	Helical; Name=7; Potential
	Transmembrane	375 – 394	20	Helical; Name=8; Potential
	Transmembrane	422 – 440	19	Helical; Name=9; Potential
	Transmembrane	457 – 477	21	Helical; Name=10; Potential
	Transmembrane	498 – 517	20	Helical; Name=11; Potential
	Transmembrane	536 – 554	19	Helical; Name=12; Potential
	Topological domain	555 – 599	45	Cytoplasmic Potential
	Motif	597 – 599	3	PDZ-binding
Amino acid modifications
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Glycosylation	181	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	117 – 118	2	KL → NW in M92378. Ref.1
	Sequence conflict	126 – 127	2	GL → AV in M92378. Ref.1
	Sequence conflict	212 – 213	2	WP → C in M92378. Ref.1
	Sequence conflict	285 – 286	2	WL → IF in M92378. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P31648 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 4FEF85092DC1D045	FASTA	599	67,001
	10 20 30 40 50 60 MATDNSKVAD GQISTEVSEA PVASDKPKTL VVKVQKKAGD LPDRDTWKGR FDFLMSCVGY 70 80 90 100 110 120 AIGLGNVWRF PYLCGKNGGG AFLIPYFLTL IFAGVPLFLL ECSLGQYTSI GGLGVWKLAP 130 140 150 160 170 180 MFKGVGLAAA VLSFWLNIYY IVIISWAIYY LYNSFTTTLP WKQCDNPWNT DRCFSNYSLV 190 200 210 220 230 240 NTTNMTSAVV EFWERNMHQM TDGLDKPGQI RWPLAITLAI AWVLVYFCIW KGVGWTGKVV 250 260 270 280 290 300 YFSATYPYIM LIILFFRGVT LPGAKEGILF YITPNFRKLS DSEVWLDAAT QIFFSYGLGL 310 320 330 340 350 360 GSLIALGSYN SFHNNVYRDS IIVCCINSCT SMFAGFVIFS IVGFMAHVTK RSIADVAASG 370 380 390 400 410 420 PGLAFLAYPE AVTQLPISPL WAILFFSMLL MLGIDSQFCT VEGFITALVD EYPRLLRNRR 430 440 450 460 470 480 ELFIAAVCIV SYLIGLSNIT QGGIYVFKLF DYYSASGMSL LFLVFFECVS ISWFYGVNRF 490 500 510 520 530 540 YDNIQEMVGS RPCIWWKLCW SFFTPIIVAG VFLFSAVQMT PLTMGSYVFP KWGQGVGWLM 550 560 570 580 590 ALSSMVLIPG YMAYMFLTLK GSLKQRLQVM IQPSEDIVRP ENGPEQPQAG SSASKEAYI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A family of genes encoding neurotransmitter transporters." Liu Q.-R., Mandiyan S., Nelson H., Nelson N. Proc. Natl. Acad. Sci. U.S.A. 89:6639-6643(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Cerebellum, Eye and Head.
[3]	"The GABA transporter GAT1 and the MAGUK protein Pals1: interaction, uptake modulation, and coexpression in the brain." McHugh E.M., Zhu W., Milgram S., Mager S. Mol. Cell. Neurosci. 26:406-417(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MPP5, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M92377 Genomic DNA. No translation available. M92378 mRNA. No translation available. AK042956 mRNA. Translation: BAC31418.1. AK052971 mRNA. Translation: BAC35226.1. AK053883 mRNA. Translation: BAC35574.1.
IPI	IPI00227928.
PIR	F46027.
RefSeq	NP_848818.1. NM_178703.4.
UniGene	Mm.5260.
3D structure databases
ProteinModelPortal	P31648.
SMR	P31648. Positions 44-532.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000032454.
PTM databases
PhosphoSite	P31648.
Proteomic databases
PaxDb	P31648.
PRIDE	P31648.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032454; ENSMUSP00000032454; ENSMUSG00000030310.
GeneID	232333.
KEGG	mmu:232333.
UCSC	uc009dhv.2. mouse.
Organism-specific databases
CTD	6529.
MGI	MGI:95627. Slc6a1.
Phylogenomic databases
eggNOG	COG0733.
GeneTree	ENSGT00690000101944.
HOGENOM	HOG000116406.
HOVERGEN	HBG071421.
InParanoid	P31648.
KO	K05034.
OMA	NSFTTEL.
OrthoDB	EOG4JQ3X4.
Gene expression databases
Bgee	P31648.
Genevestigator	P31648.
GermOnline	ENSMUSG00000030310. Mus musculus.
Family and domain databases
InterPro	IPR000175. Na/ntran_symport. IPR002980. Na/ntran_symport_GABA_GAT1. [Graphical view]
PANTHER	PTHR11616. PTHR11616. 1 hit.
Pfam	PF00209. SNF. 1 hit. [Graphical view]
PRINTS	PR01195. GAT1TRNSPORT. PR00176. NANEUSMPORT.
PROSITE	PS00610. NA_NEUROTRAN_SYMP_1. 1 hit. PS00754. NA_NEUROTRAN_SYMP_2. 1 hit. PS50267. NA_NEUROTRAN_SYMP_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P31648.
ChEMBL	CHEMBL5445.
ChiTaRS	SLC6A1. mouse.
NextBio	381034.
SOURCE	Search...

Entry information

Entry name

SC6A1_MOUSE

Accession

Primary (citable) accession number: P31648

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	May 10, 2004
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program