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P31648

- SC6A1_MOUSE

UniProt

P31648 - SC6A1_MOUSE

Protein

Sodium- and chloride-dependent GABA transporter 1

Gene

Slc6a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (10 May 2004)
      Previous versions | rss
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    Functioni

    Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

    GO - Molecular functioni

    1. gamma-aminobutyric acid:sodium symporter activity Source: Ensembl
    2. neurotransmitter:sodium symporter activity Source: InterPro

    GO - Biological processi

    1. gamma-aminobutyric acid import Source: Ensembl
    2. learning Source: Ensembl
    3. negative regulation of synaptic transmission, GABAergic Source: Ensembl
    4. positive regulation of gamma-aminobutyric acid secretion Source: Ensembl
    5. protein homooligomerization Source: Ensembl
    6. response to calcium ion Source: Ensembl
    7. response to cocaine Source: Ensembl
    8. response to estradiol Source: Ensembl
    9. response to lead ion Source: Ensembl
    10. response to purine-containing compound Source: Ensembl
    11. response to sucrose Source: Ensembl
    12. response to toxic substance Source: Ensembl

    Keywords - Biological processi

    Neurotransmitter transport, Symport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium- and chloride-dependent GABA transporter 1
    Short name:
    GAT-1
    Alternative name(s):
    Solute carrier family 6 member 1
    Gene namesi
    Name:Slc6a1
    Synonyms:Gabt1, Gat-1, Gat1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:95627. Slc6a1.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity. Membrane By similarity; Multi-pass membrane protein By similarity
    Note: Localized at the plasma membrane and in a subset of intracellular vesicles By similarity. Localized at the presynaptic terminals of interneurons.By similarity1 Publication

    GO - Cellular componenti

    1. axon Source: MGI
    2. cell surface Source: Ensembl
    3. integral component of plasma membrane Source: InterPro
    4. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 599599Sodium- and chloride-dependent GABA transporter 1PRO_0000214744Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP31648.
    PaxDbiP31648.
    PRIDEiP31648.

    PTM databases

    PhosphoSiteiP31648.

    Expressioni

    Tissue specificityi

    Brain. Expressed in the dentate gyrus of hippocampus, striatum and cerebellum (at protein level).1 Publication

    Gene expression databases

    BgeeiP31648.
    GenevestigatoriP31648.

    Interactioni

    Subunit structurei

    Interacts with MPP5.1 Publication

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000032454.

    Structurei

    3D structure databases

    ProteinModelPortaliP31648.
    SMRiP31648. Positions 44-521.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5252CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini145 – 21167ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini555 – 59945CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei53 – 7321Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei81 – 10020Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei124 – 14421Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei212 – 23019Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei239 – 25618Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei292 – 30918Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei321 – 34222Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei375 – 39420Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei422 – 44019Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei457 – 47721Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei498 – 51720Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei536 – 55419Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi597 – 5993PDZ-binding

    Domaini

    The PDZ domain-binding motif is involved in the interaction with MPP5.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0733.
    GeneTreeiENSGT00730000110773.
    HOGENOMiHOG000116406.
    HOVERGENiHBG071421.
    InParanoidiP31648.
    KOiK05034.
    OMAiFFFNIVQ.
    OrthoDBiEOG793B71.
    PhylomeDBiP31648.
    TreeFamiTF343812.

    Family and domain databases

    InterProiIPR000175. Na/ntran_symport.
    IPR002980. Na/ntran_symport_GABA_GAT1.
    [Graphical view]
    PANTHERiPTHR11616. PTHR11616. 1 hit.
    PfamiPF00209. SNF. 1 hit.
    [Graphical view]
    PRINTSiPR01195. GAT1TRNSPORT.
    PR00176. NANEUSMPORT.
    PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31648-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATDNSKVAD GQISTEVSEA PVASDKPKTL VVKVQKKAGD LPDRDTWKGR    50
    FDFLMSCVGY AIGLGNVWRF PYLCGKNGGG AFLIPYFLTL IFAGVPLFLL 100
    ECSLGQYTSI GGLGVWKLAP MFKGVGLAAA VLSFWLNIYY IVIISWAIYY 150
    LYNSFTTTLP WKQCDNPWNT DRCFSNYSLV NTTNMTSAVV EFWERNMHQM 200
    TDGLDKPGQI RWPLAITLAI AWVLVYFCIW KGVGWTGKVV YFSATYPYIM 250
    LIILFFRGVT LPGAKEGILF YITPNFRKLS DSEVWLDAAT QIFFSYGLGL 300
    GSLIALGSYN SFHNNVYRDS IIVCCINSCT SMFAGFVIFS IVGFMAHVTK 350
    RSIADVAASG PGLAFLAYPE AVTQLPISPL WAILFFSMLL MLGIDSQFCT 400
    VEGFITALVD EYPRLLRNRR ELFIAAVCIV SYLIGLSNIT QGGIYVFKLF 450
    DYYSASGMSL LFLVFFECVS ISWFYGVNRF YDNIQEMVGS RPCIWWKLCW 500
    SFFTPIIVAG VFLFSAVQMT PLTMGSYVFP KWGQGVGWLM ALSSMVLIPG 550
    YMAYMFLTLK GSLKQRLQVM IQPSEDIVRP ENGPEQPQAG SSASKEAYI 599
    Length:599
    Mass (Da):67,001
    Last modified:May 10, 2004 - v2
    Checksum:i4FEF85092DC1D045
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti117 – 1182KL → NW in M92378. (PubMed:1631167)Curated
    Sequence conflicti126 – 1272GL → AV in M92378. (PubMed:1631167)Curated
    Sequence conflicti212 – 2132WP → C in M92378. (PubMed:1631167)Curated
    Sequence conflicti285 – 2862WL → IF in M92378. (PubMed:1631167)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92377 Genomic DNA. No translation available.
    M92378 mRNA. No translation available.
    AK042956 mRNA. Translation: BAC31418.1.
    AK052971 mRNA. Translation: BAC35226.1.
    AK053883 mRNA. Translation: BAC35574.1.
    CCDSiCCDS20434.1.
    PIRiF46027.
    RefSeqiNP_848818.1. NM_178703.4.
    UniGeneiMm.5260.

    Genome annotation databases

    EnsembliENSMUST00000032454; ENSMUSP00000032454; ENSMUSG00000030310.
    GeneIDi232333.
    KEGGimmu:232333.
    UCSCiuc009dhu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92377 Genomic DNA. No translation available.
    M92378 mRNA. No translation available.
    AK042956 mRNA. Translation: BAC31418.1 .
    AK052971 mRNA. Translation: BAC35226.1 .
    AK053883 mRNA. Translation: BAC35574.1 .
    CCDSi CCDS20434.1.
    PIRi F46027.
    RefSeqi NP_848818.1. NM_178703.4.
    UniGenei Mm.5260.

    3D structure databases

    ProteinModelPortali P31648.
    SMRi P31648. Positions 44-521.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000032454.

    Chemistry

    BindingDBi P31648.
    ChEMBLi CHEMBL2096685.

    PTM databases

    PhosphoSitei P31648.

    Proteomic databases

    MaxQBi P31648.
    PaxDbi P31648.
    PRIDEi P31648.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032454 ; ENSMUSP00000032454 ; ENSMUSG00000030310 .
    GeneIDi 232333.
    KEGGi mmu:232333.
    UCSCi uc009dhu.2. mouse.

    Organism-specific databases

    CTDi 6529.
    MGIi MGI:95627. Slc6a1.

    Phylogenomic databases

    eggNOGi COG0733.
    GeneTreei ENSGT00730000110773.
    HOGENOMi HOG000116406.
    HOVERGENi HBG071421.
    InParanoidi P31648.
    KOi K05034.
    OMAi FFFNIVQ.
    OrthoDBi EOG793B71.
    PhylomeDBi P31648.
    TreeFami TF343812.

    Miscellaneous databases

    ChiTaRSi SLC6A1. mouse.
    NextBioi 381034.
    PROi P31648.
    SOURCEi Search...

    Gene expression databases

    Bgeei P31648.
    Genevestigatori P31648.

    Family and domain databases

    InterProi IPR000175. Na/ntran_symport.
    IPR002980. Na/ntran_symport_GABA_GAT1.
    [Graphical view ]
    PANTHERi PTHR11616. PTHR11616. 1 hit.
    Pfami PF00209. SNF. 1 hit.
    [Graphical view ]
    PRINTSi PR01195. GAT1TRNSPORT.
    PR00176. NANEUSMPORT.
    PROSITEi PS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A family of genes encoding neurotransmitter transporters."
      Liu Q.-R., Mandiyan S., Nelson H., Nelson N.
      Proc. Natl. Acad. Sci. U.S.A. 89:6639-6643(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum, Eye and Head.
    3. "The GABA transporter GAT1 and the MAGUK protein Pals1: interaction, uptake modulation, and coexpression in the brain."
      McHugh E.M., Zhu W., Milgram S., Mager S.
      Mol. Cell. Neurosci. 26:406-417(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MPP5, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiSC6A1_MOUSE
    AccessioniPrimary (citable) accession number: P31648
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein is the target of psychomotor stimulants such as amphetamines or cocaine.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3