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P31648

- SC6A1_MOUSE

UniProt

P31648 - SC6A1_MOUSE

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Protein

Sodium- and chloride-dependent GABA transporter 1

Gene
Slc6a1, Gabt1, Gat-1, Gat1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

GO - Molecular functioni

  1. gamma-aminobutyric acid:sodium symporter activity Source: Ensembl
  2. neurotransmitter:sodium symporter activity Source: InterPro

GO - Biological processi

  1. gamma-aminobutyric acid import Source: Ensembl
  2. learning Source: Ensembl
  3. negative regulation of synaptic transmission, GABAergic Source: Ensembl
  4. positive regulation of gamma-aminobutyric acid secretion Source: Ensembl
  5. protein homooligomerization Source: Ensembl
  6. response to calcium ion Source: Ensembl
  7. response to cocaine Source: Ensembl
  8. response to estradiol Source: Ensembl
  9. response to lead ion Source: Ensembl
  10. response to purine-containing compound Source: Ensembl
  11. response to sucrose Source: Ensembl
  12. response to toxic substance Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Symport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent GABA transporter 1
Short name:
GAT-1
Alternative name(s):
Solute carrier family 6 member 1
Gene namesi
Name:Slc6a1
Synonyms:Gabt1, Gat-1, Gat1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:95627. Slc6a1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein By similarity. Membrane; Multi-pass membrane protein By similarity
Note: Localized at the plasma membrane and in a subset of intracellular vesicles By similarity. Localized at the presynaptic terminals of interneurons.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5252Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei53 – 7321Helical; Name=1; Reviewed predictionAdd
BLAST
Transmembranei81 – 10020Helical; Name=2; Reviewed predictionAdd
BLAST
Transmembranei124 – 14421Helical; Name=3; Reviewed predictionAdd
BLAST
Topological domaini145 – 21167Extracellular Reviewed predictionAdd
BLAST
Transmembranei212 – 23019Helical; Name=4; Reviewed predictionAdd
BLAST
Transmembranei239 – 25618Helical; Name=5; Reviewed predictionAdd
BLAST
Transmembranei292 – 30918Helical; Name=6; Reviewed predictionAdd
BLAST
Transmembranei321 – 34222Helical; Name=7; Reviewed predictionAdd
BLAST
Transmembranei375 – 39420Helical; Name=8; Reviewed predictionAdd
BLAST
Transmembranei422 – 44019Helical; Name=9; Reviewed predictionAdd
BLAST
Transmembranei457 – 47721Helical; Name=10; Reviewed predictionAdd
BLAST
Transmembranei498 – 51720Helical; Name=11; Reviewed predictionAdd
BLAST
Transmembranei536 – 55419Helical; Name=12; Reviewed predictionAdd
BLAST
Topological domaini555 – 59945Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. axon Source: MGI
  2. cell surface Source: Ensembl
  3. integral component of plasma membrane Source: InterPro
  4. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 599599Sodium- and chloride-dependent GABA transporter 1PRO_0000214744Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi176 – 1761N-linked (GlcNAc...) Reviewed prediction
Glycosylationi181 – 1811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi184 – 1841N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP31648.
PaxDbiP31648.
PRIDEiP31648.

PTM databases

PhosphoSiteiP31648.

Expressioni

Tissue specificityi

Brain. Expressed in the dentate gyrus of hippocampus, striatum and cerebellum (at protein level).1 Publication

Gene expression databases

BgeeiP31648.
GenevestigatoriP31648.

Interactioni

Subunit structurei

Interacts with MPP5.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032454.

Structurei

3D structure databases

ProteinModelPortaliP31648.
SMRiP31648. Positions 44-521.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi597 – 5993PDZ-binding

Domaini

The PDZ domain-binding motif is involved in the interaction with MPP5 By similarity.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00730000110773.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP31648.
KOiK05034.
OMAiFFFNIVQ.
OrthoDBiEOG793B71.
PhylomeDBiP31648.
TreeFamiTF343812.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002980. Na/ntran_symport_GABA_GAT1.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01195. GAT1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31648-1 [UniParc]FASTAAdd to Basket

« Hide

MATDNSKVAD GQISTEVSEA PVASDKPKTL VVKVQKKAGD LPDRDTWKGR    50
FDFLMSCVGY AIGLGNVWRF PYLCGKNGGG AFLIPYFLTL IFAGVPLFLL 100
ECSLGQYTSI GGLGVWKLAP MFKGVGLAAA VLSFWLNIYY IVIISWAIYY 150
LYNSFTTTLP WKQCDNPWNT DRCFSNYSLV NTTNMTSAVV EFWERNMHQM 200
TDGLDKPGQI RWPLAITLAI AWVLVYFCIW KGVGWTGKVV YFSATYPYIM 250
LIILFFRGVT LPGAKEGILF YITPNFRKLS DSEVWLDAAT QIFFSYGLGL 300
GSLIALGSYN SFHNNVYRDS IIVCCINSCT SMFAGFVIFS IVGFMAHVTK 350
RSIADVAASG PGLAFLAYPE AVTQLPISPL WAILFFSMLL MLGIDSQFCT 400
VEGFITALVD EYPRLLRNRR ELFIAAVCIV SYLIGLSNIT QGGIYVFKLF 450
DYYSASGMSL LFLVFFECVS ISWFYGVNRF YDNIQEMVGS RPCIWWKLCW 500
SFFTPIIVAG VFLFSAVQMT PLTMGSYVFP KWGQGVGWLM ALSSMVLIPG 550
YMAYMFLTLK GSLKQRLQVM IQPSEDIVRP ENGPEQPQAG SSASKEAYI 599
Length:599
Mass (Da):67,001
Last modified:May 10, 2004 - v2
Checksum:i4FEF85092DC1D045
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1182KL → NW in M92378. 1 Publication
Sequence conflicti126 – 1272GL → AV in M92378. 1 Publication
Sequence conflicti212 – 2132WP → C in M92378. 1 Publication
Sequence conflicti285 – 2862WL → IF in M92378. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M92377 Genomic DNA. No translation available.
M92378 mRNA. No translation available.
AK042956 mRNA. Translation: BAC31418.1.
AK052971 mRNA. Translation: BAC35226.1.
AK053883 mRNA. Translation: BAC35574.1.
CCDSiCCDS20434.1.
PIRiF46027.
RefSeqiNP_848818.1. NM_178703.4.
UniGeneiMm.5260.

Genome annotation databases

EnsembliENSMUST00000032454; ENSMUSP00000032454; ENSMUSG00000030310.
GeneIDi232333.
KEGGimmu:232333.
UCSCiuc009dhu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M92377 Genomic DNA. No translation available.
M92378 mRNA. No translation available.
AK042956 mRNA. Translation: BAC31418.1 .
AK052971 mRNA. Translation: BAC35226.1 .
AK053883 mRNA. Translation: BAC35574.1 .
CCDSi CCDS20434.1.
PIRi F46027.
RefSeqi NP_848818.1. NM_178703.4.
UniGenei Mm.5260.

3D structure databases

ProteinModelPortali P31648.
SMRi P31648. Positions 44-521.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000032454.

Chemistry

BindingDBi P31648.
ChEMBLi CHEMBL2096685.

PTM databases

PhosphoSitei P31648.

Proteomic databases

MaxQBi P31648.
PaxDbi P31648.
PRIDEi P31648.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032454 ; ENSMUSP00000032454 ; ENSMUSG00000030310 .
GeneIDi 232333.
KEGGi mmu:232333.
UCSCi uc009dhu.2. mouse.

Organism-specific databases

CTDi 6529.
MGIi MGI:95627. Slc6a1.

Phylogenomic databases

eggNOGi COG0733.
GeneTreei ENSGT00730000110773.
HOGENOMi HOG000116406.
HOVERGENi HBG071421.
InParanoidi P31648.
KOi K05034.
OMAi FFFNIVQ.
OrthoDBi EOG793B71.
PhylomeDBi P31648.
TreeFami TF343812.

Miscellaneous databases

ChiTaRSi SLC6A1. mouse.
NextBioi 381034.
PROi P31648.
SOURCEi Search...

Gene expression databases

Bgeei P31648.
Genevestigatori P31648.

Family and domain databases

InterProi IPR000175. Na/ntran_symport.
IPR002980. Na/ntran_symport_GABA_GAT1.
[Graphical view ]
PANTHERi PTHR11616. PTHR11616. 1 hit.
Pfami PF00209. SNF. 1 hit.
[Graphical view ]
PRINTSi PR01195. GAT1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEi PS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A family of genes encoding neurotransmitter transporters."
    Liu Q.-R., Mandiyan S., Nelson H., Nelson N.
    Proc. Natl. Acad. Sci. U.S.A. 89:6639-6643(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum, Eye and Head.
  3. "The GABA transporter GAT1 and the MAGUK protein Pals1: interaction, uptake modulation, and coexpression in the brain."
    McHugh E.M., Zhu W., Milgram S., Mager S.
    Mol. Cell. Neurosci. 26:406-417(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MPP5, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSC6A1_MOUSE
AccessioniPrimary (citable) accession number: P31648
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 10, 2004
Last modified: July 9, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines or cocaine.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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