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P31648 (SC6A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium- and chloride-dependent GABA transporter 1

Short name=GAT-1
Alternative name(s):
Solute carrier family 6 member 1
Gene names
Name:Slc6a1
Synonyms:Gabt1, Gat-1, Gat1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length599 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

Subunit structure

Interacts with MPP5. Ref.3

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Membrane; Multi-pass membrane protein By similarity. Note: Localized at the plasma membrane and in a subset of intracellular vesicles By similarity. Localized at the presynaptic terminals of interneurons. Ref.3

Tissue specificity

Brain. Expressed in the dentate gyrus of hippocampus, striatum and cerebellum (at protein level). Ref.3

Domain

The PDZ domain-binding motif is involved in the interaction with MPP5 By similarity.

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines or cocaine.

Sequence similarities

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily. [View classification]

Ontologies

Keywords
   Biological processNeurotransmitter transport
Symport
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processgamma-aminobutyric acid import

Inferred from electronic annotation. Source: Ensembl

learning

Inferred from electronic annotation. Source: Ensembl

negative regulation of synaptic transmission, GABAergic

Inferred from electronic annotation. Source: Ensembl

positive regulation of gamma-aminobutyric acid secretion

Inferred from electronic annotation. Source: Ensembl

protein homooligomerization

Inferred from electronic annotation. Source: Ensembl

response to calcium ion

Inferred from electronic annotation. Source: Ensembl

response to cocaine

Inferred from electronic annotation. Source: Ensembl

response to estradiol

Inferred from electronic annotation. Source: Ensembl

response to lead ion

Inferred from electronic annotation. Source: Ensembl

response to purine-containing compound

Inferred from electronic annotation. Source: Ensembl

response to sucrose

Inferred from electronic annotation. Source: Ensembl

response to toxic substance

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentaxon

Inferred from direct assay PubMed 15479642. Source: MGI

cell surface

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Inferred from electronic annotation. Source: InterPro

plasma membrane

Inferred from direct assay PubMed 17724084. Source: MGI

   Molecular_functiongamma-aminobutyric acid:sodium symporter activity

Inferred from electronic annotation. Source: Ensembl

neurotransmitter:sodium symporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 599599Sodium- and chloride-dependent GABA transporter 1
PRO_0000214744

Regions

Topological domain1 – 5252Cytoplasmic Potential
Transmembrane53 – 7321Helical; Name=1; Potential
Transmembrane81 – 10020Helical; Name=2; Potential
Transmembrane124 – 14421Helical; Name=3; Potential
Topological domain145 – 21167Extracellular Potential
Transmembrane212 – 23019Helical; Name=4; Potential
Transmembrane239 – 25618Helical; Name=5; Potential
Transmembrane292 – 30918Helical; Name=6; Potential
Transmembrane321 – 34222Helical; Name=7; Potential
Transmembrane375 – 39420Helical; Name=8; Potential
Transmembrane422 – 44019Helical; Name=9; Potential
Transmembrane457 – 47721Helical; Name=10; Potential
Transmembrane498 – 51720Helical; Name=11; Potential
Transmembrane536 – 55419Helical; Name=12; Potential
Topological domain555 – 59945Cytoplasmic Potential
Motif597 – 5993PDZ-binding

Amino acid modifications

Glycosylation1761N-linked (GlcNAc...) Potential
Glycosylation1811N-linked (GlcNAc...) Potential
Glycosylation1841N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict117 – 1182KL → NW in M92378. Ref.1
Sequence conflict126 – 1272GL → AV in M92378. Ref.1
Sequence conflict212 – 2132WP → C in M92378. Ref.1
Sequence conflict285 – 2862WL → IF in M92378. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P31648 [UniParc].

Last modified May 10, 2004. Version 2.
Checksum: 4FEF85092DC1D045

FASTA59967,001
        10         20         30         40         50         60 
MATDNSKVAD GQISTEVSEA PVASDKPKTL VVKVQKKAGD LPDRDTWKGR FDFLMSCVGY 

        70         80         90        100        110        120 
AIGLGNVWRF PYLCGKNGGG AFLIPYFLTL IFAGVPLFLL ECSLGQYTSI GGLGVWKLAP 

       130        140        150        160        170        180 
MFKGVGLAAA VLSFWLNIYY IVIISWAIYY LYNSFTTTLP WKQCDNPWNT DRCFSNYSLV 

       190        200        210        220        230        240 
NTTNMTSAVV EFWERNMHQM TDGLDKPGQI RWPLAITLAI AWVLVYFCIW KGVGWTGKVV 

       250        260        270        280        290        300 
YFSATYPYIM LIILFFRGVT LPGAKEGILF YITPNFRKLS DSEVWLDAAT QIFFSYGLGL 

       310        320        330        340        350        360 
GSLIALGSYN SFHNNVYRDS IIVCCINSCT SMFAGFVIFS IVGFMAHVTK RSIADVAASG 

       370        380        390        400        410        420 
PGLAFLAYPE AVTQLPISPL WAILFFSMLL MLGIDSQFCT VEGFITALVD EYPRLLRNRR 

       430        440        450        460        470        480 
ELFIAAVCIV SYLIGLSNIT QGGIYVFKLF DYYSASGMSL LFLVFFECVS ISWFYGVNRF 

       490        500        510        520        530        540 
YDNIQEMVGS RPCIWWKLCW SFFTPIIVAG VFLFSAVQMT PLTMGSYVFP KWGQGVGWLM 

       550        560        570        580        590 
ALSSMVLIPG YMAYMFLTLK GSLKQRLQVM IQPSEDIVRP ENGPEQPQAG SSASKEAYI 

« Hide

References

« Hide 'large scale' references
[1]"A family of genes encoding neurotransmitter transporters."
Liu Q.-R., Mandiyan S., Nelson H., Nelson N.
Proc. Natl. Acad. Sci. U.S.A. 89:6639-6643(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Cerebellum, Eye and Head.
[3]"The GABA transporter GAT1 and the MAGUK protein Pals1: interaction, uptake modulation, and coexpression in the brain."
McHugh E.M., Zhu W., Milgram S., Mager S.
Mol. Cell. Neurosci. 26:406-417(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MPP5, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M92377 Genomic DNA. No translation available.
M92378 mRNA. No translation available.
AK042956 mRNA. Translation: BAC31418.1.
AK052971 mRNA. Translation: BAC35226.1.
AK053883 mRNA. Translation: BAC35574.1.
PIRF46027.
RefSeqNP_848818.1. NM_178703.4.
UniGeneMm.5260.

3D structure databases

ProteinModelPortalP31648.
SMRP31648. Positions 42-576.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000032454.

Chemistry

BindingDBP31648.
ChEMBLCHEMBL5445.

PTM databases

PhosphoSiteP31648.

Proteomic databases

PaxDbP31648.
PRIDEP31648.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032454; ENSMUSP00000032454; ENSMUSG00000030310.
GeneID232333.
KEGGmmu:232333.
UCSCuc009dhu.2. mouse.

Organism-specific databases

CTD6529.
MGIMGI:95627. Slc6a1.

Phylogenomic databases

eggNOGCOG0733.
GeneTreeENSGT00730000110773.
HOGENOMHOG000116406.
HOVERGENHBG071421.
InParanoidP31648.
KOK05034.
OMAFFFNIVQ.
OrthoDBEOG793B71.
PhylomeDBP31648.
TreeFamTF343812.

Gene expression databases

BgeeP31648.
GenevestigatorP31648.

Family and domain databases

InterProIPR000175. Na/ntran_symport.
IPR002980. Na/ntran_symport_GABA_GAT1.
[Graphical view]
PANTHERPTHR11616. PTHR11616. 1 hit.
PfamPF00209. SNF. 1 hit.
[Graphical view]
PRINTSPR01195. GAT1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC6A1. mouse.
NextBio381034.
PROP31648.
SOURCESearch...

Entry information

Entry nameSC6A1_MOUSE
AccessionPrimary (citable) accession number: P31648
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 10, 2004
Last modified: April 16, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot