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Protein

Sodium- and chloride-dependent GABA transporter 1

Gene

Slc6a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_284770. Reuptake of GABA.
REACT_307956. Na+/Cl- dependent neurotransmitter transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent GABA transporter 1
Short name:
GAT-1
Alternative name(s):
Solute carrier family 6 member 1
Gene namesi
Name:Slc6a1
Synonyms:Gabt1, Gat-1, Gat1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:95627. Slc6a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5252CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei53 – 7321Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei81 – 10020Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei124 – 14421Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini145 – 21167ExtracellularSequence AnalysisAdd
BLAST
Transmembranei212 – 23019Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei239 – 25618Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei292 – 30918Helical; Name=6Sequence AnalysisAdd
BLAST
Transmembranei321 – 34222Helical; Name=7Sequence AnalysisAdd
BLAST
Transmembranei375 – 39420Helical; Name=8Sequence AnalysisAdd
BLAST
Transmembranei422 – 44019Helical; Name=9Sequence AnalysisAdd
BLAST
Transmembranei457 – 47721Helical; Name=10Sequence AnalysisAdd
BLAST
Transmembranei498 – 51720Helical; Name=11Sequence AnalysisAdd
BLAST
Transmembranei536 – 55419Helical; Name=12Sequence AnalysisAdd
BLAST
Topological domaini555 – 59945CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • axon Source: MGI
  • cell surface Source: Ensembl
  • integral component of plasma membrane Source: InterPro
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 599599Sodium- and chloride-dependent GABA transporter 1PRO_0000214744Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP31648.
PaxDbiP31648.
PRIDEiP31648.

PTM databases

PhosphoSiteiP31648.

Expressioni

Tissue specificityi

Brain. Expressed in the dentate gyrus of hippocampus, striatum and cerebellum (at protein level).1 Publication

Gene expression databases

BgeeiP31648.
GenevisibleiP31648. MM.

Interactioni

Subunit structurei

Interacts with MPP5.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032454.

Structurei

3D structure databases

ProteinModelPortaliP31648.
SMRiP31648. Positions 44-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi597 – 5993PDZ-binding

Domaini

The PDZ domain-binding motif is involved in the interaction with MPP5.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP31648.
KOiK05034.
OMAiTCITQGG.
OrthoDBiEOG793B71.
PhylomeDBiP31648.
TreeFamiTF343812.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002980. Na/ntran_symport_GABA_GAT1.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01195. GAT1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATDNSKVAD GQISTEVSEA PVASDKPKTL VVKVQKKAGD LPDRDTWKGR
60 70 80 90 100
FDFLMSCVGY AIGLGNVWRF PYLCGKNGGG AFLIPYFLTL IFAGVPLFLL
110 120 130 140 150
ECSLGQYTSI GGLGVWKLAP MFKGVGLAAA VLSFWLNIYY IVIISWAIYY
160 170 180 190 200
LYNSFTTTLP WKQCDNPWNT DRCFSNYSLV NTTNMTSAVV EFWERNMHQM
210 220 230 240 250
TDGLDKPGQI RWPLAITLAI AWVLVYFCIW KGVGWTGKVV YFSATYPYIM
260 270 280 290 300
LIILFFRGVT LPGAKEGILF YITPNFRKLS DSEVWLDAAT QIFFSYGLGL
310 320 330 340 350
GSLIALGSYN SFHNNVYRDS IIVCCINSCT SMFAGFVIFS IVGFMAHVTK
360 370 380 390 400
RSIADVAASG PGLAFLAYPE AVTQLPISPL WAILFFSMLL MLGIDSQFCT
410 420 430 440 450
VEGFITALVD EYPRLLRNRR ELFIAAVCIV SYLIGLSNIT QGGIYVFKLF
460 470 480 490 500
DYYSASGMSL LFLVFFECVS ISWFYGVNRF YDNIQEMVGS RPCIWWKLCW
510 520 530 540 550
SFFTPIIVAG VFLFSAVQMT PLTMGSYVFP KWGQGVGWLM ALSSMVLIPG
560 570 580 590
YMAYMFLTLK GSLKQRLQVM IQPSEDIVRP ENGPEQPQAG SSASKEAYI
Length:599
Mass (Da):67,001
Last modified:May 10, 2004 - v2
Checksum:i4FEF85092DC1D045
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1182KL → NW in M92378 (PubMed:1631167).Curated
Sequence conflicti126 – 1272GL → AV in M92378 (PubMed:1631167).Curated
Sequence conflicti212 – 2132WP → C in M92378 (PubMed:1631167).Curated
Sequence conflicti285 – 2862WL → IF in M92378 (PubMed:1631167).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92377 Genomic DNA. No translation available.
M92378 mRNA. No translation available.
AK042956 mRNA. Translation: BAC31418.1.
AK052971 mRNA. Translation: BAC35226.1.
AK053883 mRNA. Translation: BAC35574.1.
CCDSiCCDS20434.1.
PIRiF46027.
RefSeqiNP_848818.1. NM_178703.4.
UniGeneiMm.5260.

Genome annotation databases

EnsembliENSMUST00000032454; ENSMUSP00000032454; ENSMUSG00000030310.
GeneIDi232333.
KEGGimmu:232333.
UCSCiuc009dhu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92377 Genomic DNA. No translation available.
M92378 mRNA. No translation available.
AK042956 mRNA. Translation: BAC31418.1.
AK052971 mRNA. Translation: BAC35226.1.
AK053883 mRNA. Translation: BAC35574.1.
CCDSiCCDS20434.1.
PIRiF46027.
RefSeqiNP_848818.1. NM_178703.4.
UniGeneiMm.5260.

3D structure databases

ProteinModelPortaliP31648.
SMRiP31648. Positions 44-521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032454.

Chemistry

BindingDBiP31648.
ChEMBLiCHEMBL5445.
GuidetoPHARMACOLOGYi929.

PTM databases

PhosphoSiteiP31648.

Proteomic databases

MaxQBiP31648.
PaxDbiP31648.
PRIDEiP31648.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032454; ENSMUSP00000032454; ENSMUSG00000030310.
GeneIDi232333.
KEGGimmu:232333.
UCSCiuc009dhu.2. mouse.

Organism-specific databases

CTDi6529.
MGIiMGI:95627. Slc6a1.

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP31648.
KOiK05034.
OMAiTCITQGG.
OrthoDBiEOG793B71.
PhylomeDBiP31648.
TreeFamiTF343812.

Enzyme and pathway databases

ReactomeiREACT_284770. Reuptake of GABA.
REACT_307956. Na+/Cl- dependent neurotransmitter transporters.

Miscellaneous databases

NextBioi381034.
PROiP31648.
SOURCEiSearch...

Gene expression databases

BgeeiP31648.
GenevisibleiP31648. MM.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002980. Na/ntran_symport_GABA_GAT1.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01195. GAT1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A family of genes encoding neurotransmitter transporters."
    Liu Q.-R., Mandiyan S., Nelson H., Nelson N.
    Proc. Natl. Acad. Sci. U.S.A. 89:6639-6643(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum, Eye and Head.
  3. "The GABA transporter GAT1 and the MAGUK protein Pals1: interaction, uptake modulation, and coexpression in the brain."
    McHugh E.M., Zhu W., Milgram S., Mager S.
    Mol. Cell. Neurosci. 26:406-417(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MPP5, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSC6A1_MOUSE
AccessioniPrimary (citable) accession number: P31648
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 10, 2004
Last modified: June 24, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines or cocaine.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.