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Reviewed, UniProtKB/Swiss-Prot P31644 (GBRA5_HUMAN)

Last modified June 16, 2009. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Gamma-aminobutyric acid receptor subunit alpha-5
Alternative name(s):
    GABA(A) receptor subunit alpha-5
Gene names
Name: GABRA5
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Subunit structure

Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.

Subcellular location

Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ligand-gated ionic channel (TC 1.A.9) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 462431Gamma-aminobutyric acid receptor subunit alpha-5
PRO_0000000444

Regions

Topological domain32 – 259228Extracellular Potential
Transmembrane260 – 28122 Potential
Transmembrane286 – 30722 Potential
Transmembrane319 – 34123 Potential
Topological domain342 – 42786Cytoplasmic Potential
Transmembrane428 – 44922 Potential

Amino acid modifications

Glycosylation451N-linked (GlcNAc...) Potential
Glycosylation1451N-linked (GlcNAc...) Potential
Glycosylation2071N-linked (GlcNAc...) Potential
Glycosylation2361N-linked (GlcNAc...) Potential
Disulfide bond173 ↔ 187 By similarity
Cross-link355Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.8

Sequences

Sequence LengthMass (Da)Tools
P31644-1 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 260A8B554113AFF9

FASTA46252,146
        10         20         30         40         50         60 
MDNGMFSGFI MIKNLLLFCI SMNLSSHFGF SQMPTSSVKD ETNDNITIFT RILDGLLDGY 

        70         80         90        100        110        120 
DNRLRPGLGE RITQVRTDIY VTSFGPVSDT EMEYTIDVFF RQSWKDERLR FKGPMQRLPL 

       130        140        150        160        170        180 
NNLLASKIWT PDTFFHNGKK SIAHNMTTPN KLLRLEDDGT LLYTMRLTIS AECPMQLEDF 

       190        200        210        220        230        240 
PMDAHACPLK FGSYAYPNSE VVYVWTNGST KSVVVAEDGS RLNQYHLMGQ TVGTENISTS 

       250        260        270        280        290        300 
TGEYTIMTAH FHLKRKIGYF VIQTYLPCIM TVILSQVSFW LNRESVPART VFGVTTVLTM 

       310        320        330        340        350        360 
TTLSISARNS LPKVAYATAM DWFIAVCYAF VFSALIEFAT VNYFTKRGWA WDGKKALEAA 

       370        380        390        400        410        420 
KIKKKREVIL NKSTNAFTTG KMSHPPNIPK EQTPAGTSNT TSVSVKPSEE KTSESKKTYN 

       430        440        450        460 
SISKIDKMSR IVFPVLFGTF NLVYWATYLN REPVIKGAAS PK 

« Hide

References

« Hide 'large scale' references
[1]"FISH ordering of reference markers and of the gene for the alpha 5 subunit of the gamma-aminobutyric acid receptor (GABRA5) within the Angelman and Prader-Willi syndrome chromosomal regions."
Knoll J.H., Sinnett D., Wagstaff J., Glatt K.A., Wilcox A.S., Whiting P.J., Wingrove P., Sikela J.M., Lalande M.
Hum. Mol. Genet. 2:183-189(1993) [PubMed: 8388764] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and expression of a cDNA encoding the human GABA-A receptor alpha 5 subunit."
Wingrove P., Hadingham K., Wafford K., Kemp J.A., Ragan C.I., Whiting P.
Biochem. Soc. Trans. 20:18S-18S(1992) [PubMed: 1321750] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[6]"Human gamma-aminobutyric acid-type A receptor alpha5 subunit gene (GABRA5): characterization and structural organization of the 5' flanking region."
Kim Y., Glatt H., Xie W., Sinnett D., Lalande M.
Genomics 42:378-387(1997) [PubMed: 9205108] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
Tissue: Fetal brain.
[7]"Homo sapiens gamma-aminobutyric acid (GABA) A receptor, alpha 5 subunit gene (GABRA5)."
Kim S.-J., Gonen D., Yang Z.-Y., Reliford A., Dy A., Leventhal B.L., Cook E.H. Jr.
Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-166; 195-241 AND 294-363.
[8]"Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry."
Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D.
Proteomics 7:868-874(2007) [PubMed: 17370265] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-355, MASS SPECTROMETRY.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Forbidden fruit - Issue 56 of March 2005

Cross-references

Sequence databases

L08485 mRNA. Translation: AAA58490.1.
AK290453 mRNA. Translation: BAF83142.1.
BT009830 mRNA. Translation: AAP88832.1.
BC111979 mRNA. Translation: AAI11980.1.
BC113422 mRNA. Translation: AAI13423.1.
AF061785 Genomic DNA. Translation: AAC31809.1.
AF228447 Genomic DNA. Translation: AAF62508.1.
AF228448 Genomic DNA. Translation: AAF62509.1.
AF228449 Genomic DNA. Translation: AAF62510.1.
IPIIPI00012471.
PIRA48933.
RefSeqNP_000801.1.
UniGeneHs.612087

3D structure databases

ModBaseSearch...

PTM databases

PhosphoSiteP31644.

Proteomic databases

PRIDEP31644.

Genome annotation databases

EnsemblENSG00000186297. Homo sapiens. [Contig view]
GeneID2558.
KEGGhsa:2558.

Organism-specific databases

GeneCardsGC15P024663.
H-InvDBHIX0026792.
HGNCHGNC:4079. GABRA5.
MIM137142. gene.
PharmGKBPA28493.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP31644.
HOVERGENP31644.
OMAP31644. GKDETND.

Gene expression databases

ArrayExpressP31644.
BgeeP31644.
CleanExHS_GABRA5.
GermOnlineENSG00000186297. Homo sapiens.

Family and domain databases

InterProIPR006028. GABAA_rcpt.
IPR001390. GABAAa_rcpt.
IPR005435. GABBAa5_rcpt.
IPR006029. Neu_channel_TM.
IPR006202. Neur_chan_lig_bd.
IPR006201. Neur_channel.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
Gene3DG3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
PANTHERPTHR18945. Neur_channel. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR01079. GABAARALPHA.
PR01618. GABAARALPHA5.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00404. Alprazolam.
DB00189. Ethchlorvynol.
DB01544. Flunitrazepam.
DB00690. Flurazepam.
DB00186. Lorazepam.
DB00371. Meprobamate.
DB00683. Midazolam.
NextBio10103.
SOURCESearch...

Entry information

Entry nameGBRA5_HUMAN
AccessionPrimary (citable) accession number: P31644
Secondary accession number(s): A8K338 expand/collapse secondary AC list , Q14DC2, Q53XL6, Q9NYT3, Q9NYT4, Q9NYT5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 16, 2009
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents