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P31638

- ACSA_CUPNH

UniProt

P31638 - ACSA_CUPNH

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Protein

Acetyl-coenzyme A synthetase

Gene
acsA, acoE, H16_A2525
Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA. Although acetate is the preferred substrate of AcsA, propionate is also used, but at a diminished rate compared with that of acetate. Fatty acids with more than three carbon atoms are usually not accepted as substrates by AcsA.1 Publication

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei317 – 3171Coenzyme A By similarity
Binding sitei397 – 3971Substrate; via amide nitrogen By similarity
Binding sitei512 – 5121Substrate By similarity
Binding sitei528 – 5281Substrate By similarity
Active sitei530 – 5301 By similarity
Binding sitei536 – 5361Coenzyme A By similarity
Binding sitei539 – 5391Substrate By similarity
Metal bindingi550 – 5501Magnesium; via carbonyl oxygen By similarity
Metal bindingi555 – 5551Magnesium; via carbonyl oxygen By similarity
Binding sitei600 – 6001Coenzyme A

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetoin catabolic process Source: UniProtKB-UniPathway
  2. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCNEC381666:GJUJ-2492-MONOMER.
MetaCyc:MONOMER-13637.
UniPathwayiUPA00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetase (EC:6.2.1.1)
Short name:
AcCoA synthetase
Short name:
Acs
Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme
Gene namesi
Name:acsA
Synonyms:acoE
Ordered Locus Names:H16_A2525
OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Taxonomic identifieri381666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000008210: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 660660Acetyl-coenzyme A synthetaseUniRule annotationPRO_0000208353Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei625 – 6251N6-acetyllysine By similarity

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.UniRule annotation

Keywords - PTMi

Acetylation

Interactioni

Protein-protein interaction databases

STRINGi381666.H16_A2525.

Structurei

3D structure databases

ProteinModelPortaliP31638.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiAHERYEN.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31638-1 [UniParc]FASTAAdd to Basket

« Hide

MSAIESVMQE HRVFNPPEGF ASQAAIPSME AYQALCDEAE RDYEGFWARH    50
ARELLHWTKP FTKVLDQSNA PFYKWFEDGE LNASYNCLDR NLQNGNADKV 100
AIVFEADDGS VTRVTYRELH GKVCRFANGL KALGIRKGDR VVIYMPMSVE 150
GVVAMQACAR LGATHSVVFG GFSAKSLQER LVDVGAVALI TADEQMRGGK 200
ALPLKAIADD ALALGGCEAV RNVIVYRRTG GKVAWTEGRD RWMEDVSAGQ 250
PDTCEAEPVS AEHPLFVLYT SGSTGKPKGV QHSTGGYLLW ALMTMKWTFD 300
IKPDDLFWCT ADIGWVTGHT YIAYGPLAAG ATQVVFEGVP TYPNAGRFWD 350
MIARHKVSIF YTAPTAIRSL IKAAEADEKI HPKQYDLSSL RLLGTVGEPI 400
NPEAWMWYYK NIGNERCPIV DTFWQTETGG HMITPLPGAT PLVPGSCTLP 450
LPGIMAAIVD ETGHDVPNGN GGILVVKRPW PAMIRTIWGD PERFRKSYFP 500
EELGGKLYLA GDGSIRDKDT GYFTIMGRID DVLNVSGHRM GTMEIESALV 550
SNPLVAEAAV VGRPDDMTGE AICAFVVLKR SRPTGEEAVK IATELRNWVG 600
KEIGPIAKPK DIRFGDNLPK TRSGKIMRRL LRSLAKGEEI TQDTSTLENP 650
AILEQLKQAQ 660
Length:660
Mass (Da):72,602
Last modified:July 1, 1993 - v1
Checksum:iEA03022F82C5C502
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97217 Genomic DNA. Translation: AAA21945.1.
AM260479 Genomic DNA. Translation: CAJ93612.1.
PIRiA45736.
RefSeqiWP_011615709.1. NC_008313.1.
YP_726980.1. NC_008313.1.

Genome annotation databases

EnsemblBacteriaiCAJ93612; CAJ93612; H16_A2525.
GeneIDi4247141.
KEGGireh:H16_A2525.
PATRICi35234555. VBIRalEut6770_2916.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97217 Genomic DNA. Translation: AAA21945.1 .
AM260479 Genomic DNA. Translation: CAJ93612.1 .
PIRi A45736.
RefSeqi WP_011615709.1. NC_008313.1.
YP_726980.1. NC_008313.1.

3D structure databases

ProteinModelPortali P31638.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 381666.H16_A2525.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAJ93612 ; CAJ93612 ; H16_A2525 .
GeneIDi 4247141.
KEGGi reh:H16_A2525.
PATRICi 35234555. VBIRalEut6770_2916.

Phylogenomic databases

eggNOGi COG0365.
HOGENOMi HOG000229981.
KOi K01895.
OMAi AHERYEN.
OrthoDBi EOG68WR2H.

Enzyme and pathway databases

UniPathwayi UPA00040 .
BioCyci CNEC381666:GJUJ-2492-MONOMER.
MetaCyc:MONOMER-13637.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and molecular characterization of the acetyl coenzyme A synthetase gene (acoE) of Alcaligenes eutrophus."
    Priefert H., Steinbuechel A.
    J. Bacteriol. 174:6590-6599(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBSTRATE SPECIFICITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Entry informationi

Entry nameiACSA_CUPNH
AccessioniPrimary (citable) accession number: P31638
Secondary accession number(s): Q0K8Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: September 3, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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