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Protein

Gag-Pro polyprotein

Gene

pro

Organism
Sheep pulmonary adenomatosis virus (Jaagsiekte sheep retrovirus) (JSRV)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Matrix protein p10: Matrix protein.Curated
Nucleocapsid protein p14: Nucleocapsid protein.Curated
Capsid protein p27: capsid protein.Curated
Protease 17 kDa: The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.PROSITE-ProRule annotationBy similarity
Protease 13 kDa: The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.PROSITE-ProRule annotationBy similarity
G-patch peptide: Enhances the activity of the reverse transcriptase. May be part of the mature RT.By similarity

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei739Protease; shared with dimeric partnerPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri507 – 524CCHC-typePROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, DNA-binding, Hydrolase, Multifunctional enzyme, Protease, Viral nucleoprotein
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag-Pro polyprotein
Cleaved into the following 8 chains:
Nucleocapsid protein-dUTPase (EC:3.6.1.23By similarity)
Short name:
NC-dUTPase
Protease 17 kDaBy similarity (EC:3.4.23.-PROSITE-ProRule annotation)
Protease 13 kDaBy similarity (EC:3.4.23.-PROSITE-ProRule annotation)
G-patch peptideBy similarity
Gene namesi
Name:pro
OrganismiSheep pulmonary adenomatosis virus (Jaagsiekte sheep retrovirus) (JSRV)
Taxonomic identifieri11746 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeBetaretrovirus
Virus hostiOvis aries (Sheep) [TaxID: 9940]
Proteomesi
  • UP000007215 Componenti: Genome

Subcellular locationi

Protease 13 kDa :
  • Virion By similarity
Protease 17 kDa :
  • Virion By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostBy similarity
ChainiPRO_00001995482 – 866Gag-Pro polyproteinAdd BLAST865
ChainiPRO_00004431822 – 99Matrix protein p10Add BLAST98
ChainiPRO_0000443183100 – 167Phosphorylated proteinAdd BLAST68
ChainiPRO_0000443184168 – 256p12Add BLAST89
ChainiPRO_0000443185257 – 477Capsid protein p27Add BLAST221
ChainiPRO_0000443186478 – 713Nucleocapsid protein-dUTPaseAdd BLAST236
ChainiPRO_0000443187714 – 866Protease 17 kDaAdd BLAST153
ChainiPRO_0000443188714 – 831Protease 13 kDaAdd BLAST118
PeptideiPRO_0000443189832 – 866G-patch peptideAdd BLAST35

Post-translational modificationi

Protease 17 kDa: Released by autocatalytic processing. The protease can undergo further autoprocessing to yield 2 shorter but enzymatically active forms of 12 kDa and 13 kDa without the GDP domain.By similarity
Gag-Pro polyprotein: Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles.By similarity
Gag-Pro polyprotein: Specific enzymatic cleavages in vivo yield mature proteins.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei99 – 100Cleavage; by viral proteaseBy similarity2
Sitei256 – 257Cleavage; by viral proteaseBy similarity2
Sitei477 – 478Cleavage; by viral proteaseBy similarity2
Sitei713 – 714Cleavage; by viral proteaseBy similarity2
Sitei831 – 832Cleavage; by viral proteaseBy similarity2

Keywords - PTMi

Lipoprotein, Myristate

Interactioni

Subunit structurei

Protease 17 kDa: Homodimer. G-patch peptide: Interacts with the reverse transcriptase/ribonuclease H. Nucleocapsid protein-dUTPase: Homotrimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP31625
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini734 – 810Peptidase A2PROSITE-ProRule annotationAdd BLAST77
Domaini821 – 866G-patchPROSITE-ProRule annotationAdd BLAST46

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi202 – 205PTAP/PSAP motifCurated4
Motifi208 – 211PPXY motifCurated4
Motifi287 – 290PTAP/PSAP motifBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi121 – 127Poly-ProSequence analysis7
Compositional biasi202 – 228Pro-richPROSITE-ProRule annotationAdd BLAST27

Domaini

Gag polyprotein: Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Gag-p12 contains two L domains: a PTAP/PSAP motif which interacts with the UEV domain of TSG101, and a PPXY motif which binds to the WW domains of the ubiquitin ligase NEDD4. Gag-p27 contains one L domain: a PTAP/PSAP motif which interacts with the UEV domain of TSG101.By similarity
Protease 17 kDa: The glycine-rich G-patch domain (GPD) is present at the C-terminus of the protease from which it is then detached by the protease itself.By similarity

Sequence similaritiesi

In the C-terminal section; belongs to the dUTPase family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri507 – 524CCHC-typePROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

OrthoDBiVOG09000135

Family and domain databases

CDDicd05482 HIV_retropepsin_like, 1 hit
cd07557 trimeric_dUTPase, 1 hit
Gene3Di1.10.1200.30, 1 hit
1.10.150.490, 1 hit
1.10.375.10, 1 hit
2.40.70.10, 1 hit
2.70.40.10, 1 hit
InterProiView protein in InterPro
IPR001969 Aspartic_peptidase_AS
IPR003322 B_retro_matrix
IPR038124 B_retro_matrix_sf
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR033704 dUTPase_trimeric
IPR000467 G_patch_dom
IPR000721 Gag_p24
IPR001995 Peptidase_A2_cat
IPR021109 Peptidase_aspartic_dom_sf
IPR034170 Retropepsin-like_cat_dom
IPR018061 Retropepsins
IPR008916 Retrov_capsid_C
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
PF01585 G-patch, 1 hit
PF02337 Gag_p10, 1 hit
PF00607 Gag_p24, 1 hit
PF00077 RVP, 1 hit
PF00098 zf-CCHC, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004265 B_retro_matrix_N, 1 hit
SMARTiView protein in SMART
SM00443 G_patch, 1 hit
SM00343 ZnF_C2HC, 2 hits
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
SSF50630 SSF50630, 1 hit
SSF51283 SSF51283, 1 hit
SSF57756 SSF57756, 2 hits
PROSITEiView protein in PROSITE
PS50175 ASP_PROT_RETROV, 1 hit
PS00141 ASP_PROTEASE, 1 hit
PS50174 G_PATCH, 1 hit
PS50158 ZF_CCHC, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform Gag-Pro polyprotein (identifier: P31625-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGHTHSRQLF VHMLSVMLKH RGITVSKTKL INFLSFIEEV CPWFPREGTV
60 70 80 90 100
NLETWKKVGE QIRTHYTLHG PEKVPVETLS FWTLIRDCLD FDNDELKRLG
110 120 130 140 150
NLLKQEEDPL HTPDSVPSYD PPPPPPPSLK MHPSDNDDSL SSTDEAELDE
160 170 180 190 200
EAAKYHQEDW GFLAQEKGAL TSKDELVECF KNLTIALQNA GIQLPSNNNT
210 220 230 240 250
FPSAPPFPPA YTPTVMAGLD PPPGFPPPSK HMSPLQKALR QAQRLGEVVS
260 270 280 290 300
DFSLAFPVFE NNNQRYYESL PFKQLKELKI ACSQYGPTAP FTIAMIESLG
310 320 330 340 350
TQALPPNDWK QTARACLSGG DYLLWKSEFF EQCARIADVN RQQGIQTSYE
360 370 380 390 400
MLIGEGPYQA TDTQLNFLPG AYAQISNAAR QAWKKLPSSS TKTEDLSKVR
410 420 430 440 450
QGPDEPYQDF VARLLDTIGK IMSDEKAGMV LAKQLAFENA NSACQAALRP
460 470 480 490 500
YRKKGDLSDF IRICADIGPS YMQGIAMAAA LQGKSIKEVL FQQQARNKKG
510 520 530 540 550
LQKSGNSGCF VCGQPGHRAA VCPQKHQTSV NTPNLCPRCK KGKHWARDCR
560 570 580 590 600
SKTDVQGNPL PPVSGNLGEG PAPGPETMLW GNTAGSKRTI ADLCRATRGS
610 620 630 640 650
AGLDLCATSY TVLTPEMGVQ TLATGVFGPL PPGTVGLLLG RSSASLKGIL
660 670 680 690 700
IHPGVIDSDY TGEIKILASA PNKIIVINAG QRIAQLLLVP LVIQGKTINR
710 720 730 740 750
DRQDKGFGSS DAYWVQNVTE ARPELELRIN ANFFRGVLDT GADISVISDK
760 770 780 790 800
YWPTTWPKQM AISTLQGIGQ TTNPEQSSSL LTWKDKDGHT GQFKPYILPY
810 820 830 840 850
LPVNLWGRDI LSKMGVYLYS PSPTVTDLML DQGLLPNQGL GKQHQGIILP
860
LDLKPNQDRK GLGCFP
Note: Produced by -1 ribosomal frameshifting between gag-pro.1 Publication
Length:866
Mass (Da):95,245
Last modified:February 28, 2018 - v3
Checksum:i50C1920E2C6C335E
GO
Isoform Gag polyprotein (identifier: P31622-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P31622.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by conventional translation.1 Publication
Length:612
Mass (Da):68,089
GO
Isoform Gag-Pro-Pol polyprotein (identifier: P31623-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P31623.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshiftings between gag-pro and pro-pol.1 Publication
Length:1,726
Mass (Da):193,421
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80216 Genomic RNA Translation: AAA89181.1
PIRiB42740 PRMVJA
RefSeqiNP_041185.1, NC_001494.1

Genome annotation databases

GeneIDi1490019
KEGGivg:1490019

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Similar proteinsi

Entry informationi

Entry nameiPRO_JSRV
AccessioniPrimary (citable) accession number: P31625
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 28, 2018
Last modified: April 25, 2018
This is version 101 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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