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Protein

Gag polyprotein

Gene

gag

Organism
Sheep pulmonary adenomatosis virus (Jaagsiekte sheep retrovirus) (JSRV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Matrix protein p10: Matrix protein.Curated
Nucleocapsid protein p14: Nucleocapsid protein.Curated
Capsid protein p27: capsid protein.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri507 – 524CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri534 – 551CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionViral nucleoprotein
Biological processHost-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 6 chains:
Gene namesi
Name:gag
OrganismiSheep pulmonary adenomatosis virus (Jaagsiekte sheep retrovirus) (JSRV)
Taxonomic identifieri11746 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeBetaretrovirus
Virus hostiOvis aries (Sheep) [TaxID: 9940]
Proteomesi
  • UP000007215 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostBy similarity
ChainiPRO_00004431212 – 612Gag polyproteinAdd BLAST611
ChainiPRO_00000408672 – 99Matrix protein p10Add BLAST98
ChainiPRO_0000040868100 – ?168Phosphorylated protein pp24Add BLAST69
ChainiPRO_0000040869?169 – 256p12Add BLAST88
ChainiPRO_0000040870257 – 477Capsid protein p27Add BLAST221
ChainiPRO_0000040871478 – 579Nucleocapsid protein p14Add BLAST102
ChainiPRO_0000040872580 – 612p4Add BLAST33

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostBy similarity1

Post-translational modificationi

Gag polyprotein: Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles.By similarity
Gag polyprotein: Specific enzymatic cleavages in vivo yield mature proteins.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei99 – 100Cleavage; by viral proteaseBy similarity2
Sitei129 – 130Cleavage; by viral proteaseBy similarity2
Sitei168 – 169Cleavage; by viral proteaseBy similarity2
Sitei256 – 257Cleavage; by viral proteaseBy similarity2
Sitei477 – 478Cleavage; by viral proteaseBy similarity2
Sitei579 – 580Cleavage; by viral proteaseBy similarity2

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Structurei

Secondary structure

1612
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi258 – 260Combined sources3
Beta strandi265 – 267Combined sources3
Helixi272 – 284Combined sources13
Helixi290 – 300Combined sources11
Helixi306 – 316Combined sources11
Helixi319 – 343Combined sources25
Helixi349 – 353Combined sources5
Helixi356 – 358Combined sources3
Helixi361 – 363Combined sources3
Beta strandi364 – 367Combined sources4
Helixi371 – 384Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V4XX-ray1.50A257-388[»]
ProteinModelPortaliP31622
SMRiP31622
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31622

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi202 – 205PTAP/PSAP motifCurated4
Motifi208 – 211PPXY motifCurated4
Motifi287 – 290PTAP/PSAP motifBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi121 – 127Poly-ProSequence analysis7
Compositional biasi202 – 228Pro-richPROSITE-ProRule annotationAdd BLAST27

Domaini

Gag polyprotein: Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Gag-p12 contains two L domains: a PTAP/PSAP motif which interacts with the UEV domain of TSG101, and a PPXY motif which binds to the WW domains of the ubiquitin ligase NEDD4. Gag-p27 contains one L domain: a PTAP/PSAP motif which interacts with the UEV domain of TSG101.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri507 – 524CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri534 – 551CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

OrthoDBiVOG0900027C

Family and domain databases

Gene3Di1.10.1200.30, 1 hit
1.10.150.490, 1 hit
1.10.375.10, 1 hit
InterProiView protein in InterPro
IPR003322 B_retro_matrix
IPR038124 B_retro_matrix_sf
IPR000721 Gag_p24
IPR008916 Retrov_capsid_C
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF02337 Gag_p10, 1 hit
PF00607 Gag_p24, 1 hit
PF00098 zf-CCHC, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004265 B_retro_matrix_N, 1 hit
SMARTiView protein in SMART
SM00343 ZnF_C2HC, 2 hits
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
SSF57756 SSF57756, 2 hits
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform Gag polyprotein (identifier: P31622-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGHTHSRQLF VHMLSVMLKH RGITVSKTKL INFLSFIEEV CPWFPREGTV
60 70 80 90 100
NLETWKKVGE QIRTHYTLHG PEKVPVETLS FWTLIRDCLD FDNDELKRLG
110 120 130 140 150
NLLKQEEDPL HTPDSVPSYD PPPPPPPSLK MHPSDNDDSL SSTDEAELDE
160 170 180 190 200
EAAKYHQEDW GFLAQEKGAL TSKDELVECF KNLTIALQNA GIQLPSNNNT
210 220 230 240 250
FPSAPPFPPA YTPTVMAGLD PPPGFPPPSK HMSPLQKALR QAQRLGEVVS
260 270 280 290 300
DFSLAFPVFE NNNQRYYESL PFKQLKELKI ACSQYGPTAP FTIAMIESLG
310 320 330 340 350
TQALPPNDWK QTARACLSGG DYLLWKSEFF EQCARIADVN RQQGIQTSYE
360 370 380 390 400
MLIGEGPYQA TDTQLNFLPG AYAQISNAAR QAWKKLPSSS TKTEDLSKVR
410 420 430 440 450
QGPDEPYQDF VARLLDTIGK IMSDEKAGMV LAKQLAFENA NSACQAALRP
460 470 480 490 500
YRKKGDLSDF IRICADIGPS YMQGIAMAAA LQGKSIKEVL FQQQARNKKG
510 520 530 540 550
LQKSGNSGCF VCGQPGHRAA VCPQKHQTSV NTPNLCPRCK KGKHWARDCR
560 570 580 590 600
SKTDVQGNPL PPVSGNWVRG QPLAPKQCYG ATLQVPKEPL QTSVEPQEAA
610
RDWTSVPPPI QY
Note: Produced by conventional translation.1 Publication
Length:612
Mass (Da):68,089
Last modified:July 1, 1993 - v1
Checksum:i4CC6803A033EEFCC
GO
Isoform Gag-Pro polyprotein (identifier: P31625-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P31625.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting between gag-pro.1 Publication
Length:866
Mass (Da):95,245
GO
Isoform Gag-Pro-Pol polyprotein (identifier: P31623-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P31623.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshiftings between gag-pro and pro-pol.1 Publication
Length:1,726
Mass (Da):193,421
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti3H → Q in strain:JSRV-21. 1 Publication1
Natural varianti28T → P in strain:JSRV-21. 1 Publication1
Natural varianti116V → G in strain:JSRV-21. 1 Publication1
Natural varianti139S → L in strain:JSRV-21. 1 Publication1
Natural varianti193 – 197QLPSN → SLPH in strain:JSRV-21. 1 Publication5
Natural varianti214T → S in strain:JSRV-21. 1 Publication1
Natural varianti237K → R in strain:JSRV-21. 1 Publication1
Natural varianti298S → N in strain:JSRV-21. 1 Publication1
Natural varianti385K → R in strain:JSRV-21. 1 Publication1
Natural varianti526 – 529HQTS → QQGP in strain:JSRV-21. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80216 Genomic RNA Translation: AAA89180.1
AF105220 Genomic DNA Translation: AAD45224.1
PIRiA42740 FOMVJA
RefSeqiNP_041184.1, NC_001494.1 [P31622-1]

Genome annotation databases

GeneIDi1490018
KEGGivg:1490018

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Similar proteinsi

Entry informationi

Entry nameiGAG_JSRV
AccessioniPrimary (citable) accession number: P31622
Secondary accession number(s): Q9WR75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 25, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

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