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P31615

- HEMA_CVMA5

UniProt

P31615 - HEMA_CVMA5

Protein

Hemagglutinin-esterase

Gene

HE

Organism
Murine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.By similarity

    Catalytic activityi

    N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei42 – 421NucleophileBy similarity
    Active sitei339 – 3391Charge relay systemBy similarity
    Active sitei342 – 3421Charge relay systemBy similarity

    GO - Molecular functioni

    1. sialate O-acetylesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: InterPro

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-esterase (EC:3.1.1.53)
    Short name:
    HE protein
    Alternative name(s):
    E3 glycoprotein
    Gene namesi
    Name:HE
    ORF Names:2b
    OrganismiMurine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus)
    Taxonomic identifieri11142 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiMus musculus (Mouse) [TaxID: 10090]
    ProteomesiUP000007192: Genome

    Subcellular locationi

    Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
    Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 428408Hemagglutinin-esterasePRO_0000037145Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi46 ↔ 67By similarity
    Glycosylationi91 – 911N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi115 ↔ 164By similarity
    Glycosylationi149 – 1491N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi193 – 1931N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi199 ↔ 288By similarity
    Disulfide bondi207 ↔ 261By similarity
    Glycosylationi243 – 2431N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi313 – 3131N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi319 ↔ 324By similarity
    Glycosylationi328 – 3281N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi332 – 3321N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi357 – 3571N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi360 ↔ 384By similarity
    Glycosylationi371 – 3711N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    N-glycosylated in the RER.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

    Structurei

    3D structure databases

    ProteinModelPortaliP31615.
    SMRiP31615. Positions 23-389.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini21 – 404384Virion surfaceSequence AnalysisAdd
    BLAST
    Topological domaini426 – 4283IntravirionSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei405 – 42521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni9 – 129121Esterase domain first partBy similarityAdd
    BLAST
    Regioni130 – 278149Receptor bindingBy similarityAdd
    BLAST
    Regioni279 – 392114Esterase domain second partBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view]
    PfamiPF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view]
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31615-1 [UniParc]FASTAAdd to Basket

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    MCIAMAPRTL LLLIXCQLVF GFNEPLNIVS HLNDDWFLFG DSRSDCTYVE    50
    NNGHPKLDWL DLDPKLCNSG KISAKSGNSL FRSFHFTDFY NYTGEGDQIV 100
    FYEGVNFSPS HGFKCLAHGD NKRWMGNKAR FYARVYEKMA QYRSLSFVNV 150
    SYAYGGNAKP ASICKDNTLT LNNPTFISKE SNYVDYYYES EANFTLEGCD 200
    EFIVPLCGFN GHSKGSSSDA ANKYYTDSQS YYNMDIGVLY GFNSTLDVGN 250
    TAKDPGLDLT CRYLALTPGN YKAVSLEYLL SLPSKAICLH KTKRFMPVQV 300
    VDSRWSSIRQ SDNMTAAACQ LPYCFFRNTS ANYSGGTHDA HHGDFHFRQL 350
    LSGLLYNVSC IAQQGAFLYN NVSSSWPAYG YGHCPTAANI GYMAPVCIYD 400
    PLPVILLGVL LGIAVLIIVF LNVLFYDG 428
    Length:428
    Mass (Da):47,808
    Last modified:May 30, 2000 - v2
    Checksum:i0CB408413DFB20EC
    GO

    Sequence cautioni

    The sequence AF029248 differs from that shown. Reason: Frameshift at position 427.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23256 Genomic RNA. Translation: AAA46449.1. Sequence problems.
    AF029248 Genomic RNA. No translation available.
    PIRiB31165. HMIHMH.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23256 Genomic RNA. Translation: AAA46449.1 . Sequence problems.
    AF029248 Genomic RNA. No translation available.
    PIRi B31165. HMIHMH.

    3D structure databases

    ProteinModelPortali P31615.
    SMRi P31615. Positions 23-389.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view ]
    Pfami PF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of mouse hepatitis virus A59 mRNA 2: indications for RNA recombination between coronaviruses and influenza C virus."
      Luytjes W., Bredenbeek P.J., Noten A.F.H., Horzinek M.C., Spaan W.J.M.
      Virology 166:415-422(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Altered pathogenesis of a mutant of the murine coronavirus MHV-A59 is associated with a Q159L amino acid substitution in the spike protein."
      Leparc-Goffart I., Hingley S.T., Chua M.M., Jiang X., Lavi E., Weiss S.R.
      Virology 239:1-10(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate C12 mutant.

    Entry informationi

    Entry nameiHEMA_CVMA5
    AccessioniPrimary (citable) accession number: P31615
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Readthrough of the terminator UGA may occur between the codons for Ile-14 and Cys-16.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3