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P31615

- HEMA_CVMA5

UniProt

P31615 - HEMA_CVMA5

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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Murine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.By similarity

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei42 – 421NucleophileBy similarity
Active sitei339 – 3391Charge relay systemBy similarity
Active sitei342 – 3421Charge relay systemBy similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2b
OrganismiMurine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus)
Taxonomic identifieri11142 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
ProteomesiUP000007192: Genome

Subcellular locationi

Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.By similarity

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 428408Hemagglutinin-esterasePRO_0000037145Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 67By similarity
Glycosylationi91 – 911N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi115 ↔ 164By similarity
Glycosylationi149 – 1491N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi193 – 1931N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi199 ↔ 288By similarity
Disulfide bondi207 ↔ 261By similarity
Glycosylationi243 – 2431N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi313 – 3131N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi319 ↔ 324By similarity
Glycosylationi328 – 3281N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi332 – 3321N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi357 – 3571N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi360 ↔ 384By similarity
Glycosylationi371 – 3711N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

N-glycosylated in the RER.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliP31615.
SMRiP31615. Positions 23-389.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 404384Virion surfaceSequence AnalysisAdd
BLAST
Topological domaini426 – 4283IntravirionSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei405 – 42521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni9 – 129121Esterase domain first partBy similarityAdd
BLAST
Regioni130 – 278149Receptor bindingBy similarityAdd
BLAST
Regioni279 – 392114Esterase domain second partBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31615-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCIAMAPRTL LLLIXCQLVF GFNEPLNIVS HLNDDWFLFG DSRSDCTYVE
60 70 80 90 100
NNGHPKLDWL DLDPKLCNSG KISAKSGNSL FRSFHFTDFY NYTGEGDQIV
110 120 130 140 150
FYEGVNFSPS HGFKCLAHGD NKRWMGNKAR FYARVYEKMA QYRSLSFVNV
160 170 180 190 200
SYAYGGNAKP ASICKDNTLT LNNPTFISKE SNYVDYYYES EANFTLEGCD
210 220 230 240 250
EFIVPLCGFN GHSKGSSSDA ANKYYTDSQS YYNMDIGVLY GFNSTLDVGN
260 270 280 290 300
TAKDPGLDLT CRYLALTPGN YKAVSLEYLL SLPSKAICLH KTKRFMPVQV
310 320 330 340 350
VDSRWSSIRQ SDNMTAAACQ LPYCFFRNTS ANYSGGTHDA HHGDFHFRQL
360 370 380 390 400
LSGLLYNVSC IAQQGAFLYN NVSSSWPAYG YGHCPTAANI GYMAPVCIYD
410 420
PLPVILLGVL LGIAVLIIVF LNVLFYDG
Length:428
Mass (Da):47,808
Last modified:May 30, 2000 - v2
Checksum:i0CB408413DFB20EC
GO

Sequence cautioni

The sequence AF029248 differs from that shown. Reason: Frameshift at position 427.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23256 Genomic RNA. Translation: AAA46449.1. Sequence problems.
AF029248 Genomic RNA. No translation available.
PIRiB31165. HMIHMH.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23256 Genomic RNA. Translation: AAA46449.1 . Sequence problems.
AF029248 Genomic RNA. No translation available.
PIRi B31165. HMIHMH.

3D structure databases

ProteinModelPortali P31615.
SMRi P31615. Positions 23-389.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence of mouse hepatitis virus A59 mRNA 2: indications for RNA recombination between coronaviruses and influenza C virus."
    Luytjes W., Bredenbeek P.J., Noten A.F.H., Horzinek M.C., Spaan W.J.M.
    Virology 166:415-422(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Altered pathogenesis of a mutant of the murine coronavirus MHV-A59 is associated with a Q159L amino acid substitution in the spike protein."
    Leparc-Goffart I., Hingley S.T., Chua M.M., Jiang X., Lavi E., Weiss S.R.
    Virology 239:1-10(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate C12 mutant.

Entry informationi

Entry nameiHEMA_CVMA5
AccessioniPrimary (citable) accession number: P31615
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 30, 2000
Last modified: October 29, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Readthrough of the terminator UGA may occur between the codons for Ile-14 and Cys-16.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3