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P31615

- HEMA_CVMA5

UniProt

P31615 - HEMA_CVMA5

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Protein
Hemagglutinin-esterase
Gene
HE, 2b
Organism
Murine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei42 – 421Nucleophile By similarity
Active sitei235 – 2351Charge relay system By similarity
Active sitei342 – 3421Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2b
OrganismiMurine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus)
Taxonomic identifieri11142 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
ProteomesiUP000007192: Genome

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host cell membrane; Single-pass type I membrane protein Reviewed prediction
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 404384Virion surface Reviewed prediction
Add
BLAST
Transmembranei405 – 42521Helical; Reviewed prediction
Add
BLAST
Topological domaini426 – 4283Intravirion Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed prediction
Add
BLAST
Chaini21 – 428408Hemagglutinin-esterase
PRO_0000037145Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 67 By similarity
Glycosylationi91 – 911N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi115 ↔ 164 By similarity
Glycosylationi149 – 1491N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi193 – 1931N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi199 ↔ 288 By similarity
Disulfide bondi207 ↔ 261 By similarity
Glycosylationi243 – 2431N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi313 – 3131N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi319 ↔ 324 By similarity
Glycosylationi328 – 3281N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi332 – 3321N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi357 – 3571N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi360 ↔ 384 By similarity
Glycosylationi371 – 3711N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

N-glycosylated in the RER.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliP31615.
SMRiP31615. Positions 23-389.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni9 – 129121Esterase domain first part By similarity
Add
BLAST
Regioni130 – 278149Receptor binding By similarity
Add
BLAST
Regioni279 – 392114Esterase domain second part By similarity
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31615-1 [UniParc]FASTAAdd to Basket

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MCIAMAPRTL LLLIXCQLVF GFNEPLNIVS HLNDDWFLFG DSRSDCTYVE    50
NNGHPKLDWL DLDPKLCNSG KISAKSGNSL FRSFHFTDFY NYTGEGDQIV 100
FYEGVNFSPS HGFKCLAHGD NKRWMGNKAR FYARVYEKMA QYRSLSFVNV 150
SYAYGGNAKP ASICKDNTLT LNNPTFISKE SNYVDYYYES EANFTLEGCD 200
EFIVPLCGFN GHSKGSSSDA ANKYYTDSQS YYNMDIGVLY GFNSTLDVGN 250
TAKDPGLDLT CRYLALTPGN YKAVSLEYLL SLPSKAICLH KTKRFMPVQV 300
VDSRWSSIRQ SDNMTAAACQ LPYCFFRNTS ANYSGGTHDA HHGDFHFRQL 350
LSGLLYNVSC IAQQGAFLYN NVSSSWPAYG YGHCPTAANI GYMAPVCIYD 400
PLPVILLGVL LGIAVLIIVF LNVLFYDG 428
Length:428
Mass (Da):47,808
Last modified:May 30, 2000 - v2
Checksum:i0CB408413DFB20EC
GO

Sequence cautioni

The sequence AF029248 differs from that shown. Reason: Frameshift at position 427.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23256 Genomic RNA. Translation: AAA46449.1. Sequence problems.
AF029248 Genomic RNA. No translation available.
PIRiB31165. HMIHMH.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23256 Genomic RNA. Translation: AAA46449.1 . Sequence problems.
AF029248 Genomic RNA. No translation available.
PIRi B31165. HMIHMH.

3D structure databases

ProteinModelPortali P31615.
SMRi P31615. Positions 23-389.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence of mouse hepatitis virus A59 mRNA 2: indications for RNA recombination between coronaviruses and influenza C virus."
    Luytjes W., Bredenbeek P.J., Noten A.F.H., Horzinek M.C., Spaan W.J.M.
    Virology 166:415-422(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Altered pathogenesis of a mutant of the murine coronavirus MHV-A59 is associated with a Q159L amino acid substitution in the spike protein."
    Leparc-Goffart I., Hingley S.T., Chua M.M., Jiang X., Lavi E., Weiss S.R.
    Virology 239:1-10(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate C12 mutant.

Entry informationi

Entry nameiHEMA_CVMA5
AccessioniPrimary (citable) accession number: P31615
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 30, 2000
Last modified: April 16, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Readthrough of the terminator UGA may occur between the codons for Ile-14 and Cys-16.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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