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P31614

- HEMA_CVMS

UniProt

P31614 - HEMA_CVMS

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Protein
Hemagglutinin-esterase
Gene
HE, 2b
Organism
Murine coronavirus (strain S) (MHV-S) (Murine hepatitis virus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei42 – 421Nucleophile By similarity
Active sitei235 – 2351Charge relay system By similarity
Active sitei342 – 3421Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2b
OrganismiMurine coronavirus (strain S) (MHV-S) (Murine hepatitis virus)
Taxonomic identifieri11145 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host cell membrane; Single-pass type I membrane protein Reviewed prediction
Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 404385Virion surface Reviewed prediction
Add
BLAST
Transmembranei405 – 42521Helical; Reviewed prediction
Add
BLAST
Topological domaini426 – 4305Intravirion Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 430411Hemagglutinin-esterase
PRO_0000037148Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 67 By similarity
Glycosylationi91 – 911N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi115 ↔ 164 By similarity
Glycosylationi149 – 1491N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi193 – 1931N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi199 ↔ 288 By similarity
Disulfide bondi207 ↔ 261 By similarity
Glycosylationi243 – 2431N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi306 – 3061N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi313 – 3131N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi319 ↔ 324 By similarity
Glycosylationi328 – 3281N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi357 – 3571N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi360 ↔ 384 By similarity
Glycosylationi371 – 3711N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

N-glycosylated in the RER.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 283
Beta strandi32 – 343
Beta strandi36 – 427
Helixi58 – 603
Turni65 – 684
Beta strandi72 – 743
Helixi80 – 856
Beta strandi86 – 883
Beta strandi93 – 953
Beta strandi97 – 1037
Helixi121 – 13818
Turni139 – 1413
Beta strandi142 – 1498
Beta strandi173 – 1775
Beta strandi184 – 1863
Beta strandi190 – 1967
Beta strandi198 – 21114
Beta strandi217 – 2193
Beta strandi228 – 2336
Turni234 – 2363
Beta strandi238 – 2436
Beta strandi250 – 2545
Beta strandi258 – 2669
Beta strandi268 – 28215
Beta strandi284 – 2929
Beta strandi298 – 3025
Helixi314 – 3185
Turni321 – 3233
Beta strandi324 – 3274
Helixi347 – 3537
Beta strandi359 – 3624
Beta strandi365 – 3684
Beta strandi370 – 3723
Beta strandi381 – 3833
Beta strandi396 – 3983

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4C7LX-ray2.10A/B22-400[»]
4C7WX-ray2.50A/B22-400[»]
ProteinModelPortaliP31614.
SMRiP31614. Positions 23-389.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni9 – 129121Esterase domain first part By similarity
Add
BLAST
Regioni130 – 278149Receptor binding By similarity
Add
BLAST
Regioni279 – 392114Esterase domain second part By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi213 – 2186Poly-Ser

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31614-1 [UniParc]FASTAAdd to Basket

« Hide

MCIAMAPRTL LLLIGCQLVF GFNEPLNIVS HLNDDWFLFG DSRSDCTYVE    50
NNGHPKLDWL DLDPKLCNSG RIYAKSGNSL FRSFHFIDFY NYSGEGDQVI 100
FYEGVNFSPS HGFKCLAYGD NKRWMGNKAR FYARVYEKMA QYRSLSFVNV 150
SYAYGGNAKP TSICKDKTLT LNNPTFISKE SNYVDYYYES EANFTLQGCD 200
EFIVTLCVSN GHSKSSSSDP ANKYYTDAQS YYNMDTGVLY GFNSTLDVGN 250
TVQNPGLDLT CRYLALTPGN YKAVSLEYLL SLPSKAICLR KPKSFMPVQV 300
VDSRWNSTRQ SDNMTAVACQ LPYCFFRNTS ADYSGGTHDV HHGDFHFRQL 350
LSGLLYNVSC IAQQGAFVYN NVSSSWPAYG YGHCPTAANI GYMAPVCIYE 400
PLPVILLGVL LGIAVLIIVF LMFYFMTDSG 430
Length:430
Mass (Da):48,256
Last modified:July 1, 1993 - v1
Checksum:iD0401D7D5E2B8C38
GO

Sequence cautioni

The sequence AAA46460.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64316 Genomic RNA. Translation: AAA46460.1. Different initiation.
PIRiA40476. HMIHMS.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64316 Genomic RNA. Translation: AAA46460.1 . Different initiation.
PIRi A40476. HMIHMS.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4C7L X-ray 2.10 A/B 22-400 [» ]
4C7W X-ray 2.50 A/B 22-400 [» ]
ProteinModelPortali P31614.
SMRi P31614. Positions 23-389.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Heterogeneity of gene expression of the hemagglutinin-esterase (HE) protein of murine coronaviruses."
    Yokomori K., Banner L.R., Lai M.M.C.
    Virology 183:647-657(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "The sialate-4-O-acetylesterases of coronaviruses related to mouse hepatitis virus: a proposal to reorganize group 2 Coronaviridae."
    Wurzer W.J., Obojes K., Vlasak R.
    J. Gen. Virol. 83:395-402(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  3. "Nidovirus sialate-O-acetylesterases: evolution and substrate specificity of coronaviral and toroviral receptor-destroying enzymes."
    Smits S.L., Gerwig G.J., van Vliet A.L., Lissenberg A., Briza P., Kamerling J.P., Vlasak R., de Groot R.J.
    J. Biol. Chem. 280:6933-6941(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHEMA_CVMS
AccessioniPrimary (citable) accession number: P31614
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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