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Protein

Bacteriophage N4 adsorption protein A

Gene

nfrA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

(Microbial infection) Allows N4 phage attachment by binding to the viral non-contractile sheath protein.1 Publication

Enzyme and pathway databases

BioCyciEcoCyc:EG11740-MONOMER.
ECOL316407:JW0557-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacteriophage N4 adsorption protein A
Gene namesi
Name:nfrA
Ordered Locus Names:b0568, JW0557
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11740. nfrA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 990963Bacteriophage N4 adsorption protein APRO_0000021809Add
BLAST

Proteomic databases

PaxDbiP31600.
PRIDEiP31600.

Interactioni

Subunit structurei

(Microbial infection) Interacts with N4 phage non-contractile sheath protein; this interaction is essential for viral adsorption to the host.1 Publication

Protein-protein interaction databases

BioGridi4259890. 192 interactions.
DIPiDIP-10332N.
IntActiP31600. 10 interactions.
STRINGi511145.b0568.

Structurei

3D structure databases

ProteinModelPortaliP31600.
SMRiP31600. Positions 357-411, 545-715.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati81 – 11434TPR 1Add
BLAST
Repeati612 – 64534TPR 2Add
BLAST
Repeati646 – 67934TPR 3Add
BLAST

Sequence similaritiesi

Contains 3 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiENOG41090FC. Bacteria.
COG0457. LUCA.
HOGENOMiHOG000117813.
KOiK11739.
OMAiWTGESHY.
OrthoDBiEOG6PW1ZS.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR025137. NfrA_C.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13283. NfrA_C. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 2 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 4 hits.
PROSITEiPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31600-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKENNLNRVI GWSGLLLTSL LSTSALADNI GTSAEELGLS DYRHFVIYPR
60 70 80 90 100
LDKALKAQKN NDEATAIREF EYIHQQVPDN IPLTLYLAEA YRHFGHDDRA
110 120 130 140 150
RLLLEDQLKR HPGDARLERS LAAIPVEVKS VTTVEELLAQ QKACDAAPTL
160 170 180 190 200
RCRSEVGQNA LRLAQLPVAR AQLNDATFAA SPEGKTLRTD LLQRAIYLKQ
210 220 230 240 250
WSQADTLYNE ARQQNTLSAA ERRQWFDVLL AGQLDDRILA LQSQGIFTDP
260 270 280 290 300
QSYITYATAL AYRGEKARLQ HYLIENKPLF TTDAQEKSWL YLLSKYSANP
310 320 330 340 350
VQALANYTVQ FADNRQYVVG ATLPVLLKEG QYDAAQKLLA TLPANEMLEE
360 370 380 390 400
RYAVSVATRN KAEALRLARL LYQQEPANLT RLDQLTWQLM QNEQSREAAD
410 420 430 440 450
LLLQRYPFQG DARVSQTLMA RLASLLESHP YLATPAKVAI LSKPLPLAEQ
460 470 480 490 500
RQWQSQLPGI ADNCPAIVRL LGDMSPSYDA AAWNRLAKCY RDTLPGVALY
510 520 530 540 550
AWLQAEQRQP SAWQHRAVAY QAYQVEDYAT ALAAWQKISL HDMSNEDLLA
560 570 580 590 600
AANTAQAAGN GAARDRWLQQ AEKRGLGSNA LYWWLHAQRY IPGQPELALN
610 620 630 640 650
DLTRSINIAP SANAYVARAT IYRQRHNVPA AVSDLRAALE LEPNNSNTQA
660 670 680 690 700
ALGYALWDSG DIAQSREMLE PAHKGLPDDP ALIRQLAYVN QRLDDMPATQ
710 720 730 740 750
HYARLVIDDI DNQALITPLT PEQNQQRFNF RRLHEEVGRR WTFSFDSSIG
760 770 780 790 800
LRSGAMSTAN NNVGGAAPGK SYRSYGQLEA EYRIGRNMLL EGDLLSVYSR
810 820 830 840 850
VFADTGENGV MMPVKNPMSG TGLRWKPLRD QIFFIAVEQQ LPLNGQNGAS
860 870 880 890 900
DTMLRASASF FNGGKYSDEW HPNGSGWFAQ NLYLDAAQYI RQDIQAWTAD
910 920 930 940 950
YRVSWHQKVA NGQTIEPYAH VQDNGYRDKG TQGAQLGGVG VRWNIWTGET
960 970 980 990
HYDAWPHKVS LGVEYQHTFK AINQRNGERN NAFLTIGVHW
Length:990
Mass (Da):111,308
Last modified:July 1, 1993 - v1
Checksum:iAAA616665D1C2E72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16945 Unassigned DNA. Translation: AAC36849.1.
U82598 Genomic DNA. Translation: AAB40766.1.
U00096 Genomic DNA. Translation: AAC73669.1.
AP009048 Genomic DNA. Translation: BAA35202.1.
PIRiB49351.
RefSeqiNP_415100.1. NC_000913.3.
WP_000662357.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73669; AAC73669; b0568.
BAA35202; BAA35202; BAA35202.
GeneIDi944741.
KEGGiecj:JW0557.
eco:b0568.
PATRICi32116304. VBIEscCol129921_0592.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16945 Unassigned DNA. Translation: AAC36849.1.
U82598 Genomic DNA. Translation: AAB40766.1.
U00096 Genomic DNA. Translation: AAC73669.1.
AP009048 Genomic DNA. Translation: BAA35202.1.
PIRiB49351.
RefSeqiNP_415100.1. NC_000913.3.
WP_000662357.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP31600.
SMRiP31600. Positions 357-411, 545-715.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259890. 192 interactions.
DIPiDIP-10332N.
IntActiP31600. 10 interactions.
STRINGi511145.b0568.

Proteomic databases

PaxDbiP31600.
PRIDEiP31600.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73669; AAC73669; b0568.
BAA35202; BAA35202; BAA35202.
GeneIDi944741.
KEGGiecj:JW0557.
eco:b0568.
PATRICi32116304. VBIEscCol129921_0592.

Organism-specific databases

EchoBASEiEB1691.
EcoGeneiEG11740. nfrA.

Phylogenomic databases

eggNOGiENOG41090FC. Bacteria.
COG0457. LUCA.
HOGENOMiHOG000117813.
KOiK11739.
OMAiWTGESHY.
OrthoDBiEOG6PW1ZS.

Enzyme and pathway databases

BioCyciEcoCyc:EG11740-MONOMER.
ECOL316407:JW0557-MONOMER.

Miscellaneous databases

PROiP31600.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR025137. NfrA_C.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF13283. NfrA_C. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 2 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 4 hits.
PROSITEiPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two overlapping genes encoding membrane proteins required for bacteriophage N4 adsorption."
    Kiino D.R., Singer M.S., Rothman-Denes L.B.
    J. Bacteriol. 175:7081-7085(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "The tail sheath of bacteriophage N4 interacts with the Escherichia coli receptor."
    McPartland J., Rothman-Denes L.B.
    J. Bacteriol. 191:525-532(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH N4 PHAGE NON-CONTRACTILE SHEATH PROTEIN (MICROBIAL INFECTION), FUNCTION (MICROBIAL INFECTION).

Entry informationi

Entry nameiNFRA_ECOLI
AccessioniPrimary (citable) accession number: P31600
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 11, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.