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Protein

Excitatory amino acid transporter 2

Gene

Slc1a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

GO - Molecular functioni

  • high-affinity glutamate transmembrane transporter activity Source: RGD

GO - Biological processi

  • L-glutamate transport Source: RGD
  • neurotransmitter transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Protein family/group databases

TCDBi2.A.23.2.2. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 2
Alternative name(s):
GLT-1
Sodium-dependent glutamate/aspartate transporter 2
Short name:
GLUT-R
Solute carrier family 1 member 2
Gene namesi
Name:Slc1a2
Synonyms:Eaat2, Glt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3697. Slc1a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4444CytoplasmicSequence analysisAdd
BLAST
Transmembranei45 – 6420HelicalSequence analysisAdd
BLAST
Transmembranei88 – 10821HelicalSequence analysisAdd
BLAST
Transmembranei121 – 14222HelicalSequence analysisAdd
BLAST
Topological domaini143 – 23896ExtracellularSequence analysisAdd
BLAST
Transmembranei239 – 25820HelicalSequence analysisAdd
BLAST
Transmembranei279 – 30022HelicalSequence analysisAdd
BLAST
Transmembranei316 – 33823HelicalSequence analysisAdd
BLAST
Transmembranei405 – 42925HelicalSequence analysisAdd
BLAST
Transmembranei436 – 45823HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • dendritic shaft Source: RGD
  • dendritic spine Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • neuron projection Source: RGD
  • plasma membrane Source: RGD
  • presynaptic membrane Source: RGD
  • synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi298 – 2981K → H or R: Normal transporter activity. 1 Publication
Mutagenesisi298 – 2981K → N or T: Reduced transporter activity. 1 Publication
Mutagenesisi326 – 3261H → N, T, K or R: No transporter activity. 1 Publication

Chemistry

ChEMBLiCHEMBL2900.
GuidetoPHARMACOLOGYi869.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 573573Excitatory amino acid transporter 2PRO_0000202063Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3 – 31PhosphoserineCombined sources
Modified residuei21 – 211PhosphoserineCombined sources
Modified residuei24 – 241PhosphoserineCombined sources
Modified residuei25 – 251PhosphoserineCombined sources
Lipidationi38 – 381S-palmitoyl cysteineBy similarity
Glycosylationi205 – 2051N-linked (GlcNAc...)Sequence analysis
Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence analysis
Modified residuei505 – 5051PhosphoserineBy similarity
Modified residuei520 – 5201PhosphoserineBy similarity
Modified residuei531 – 5311PhosphoserineBy similarity
Modified residuei533 – 5331PhosphoserineBy similarity
Modified residuei538 – 5381PhosphotyrosineBy similarity
Modified residuei543 – 5431PhosphoserineBy similarity
Modified residuei559 – 5591PhosphoserineBy similarity
Modified residuei563 – 5631PhosphoserineBy similarity

Post-translational modificationi

Glycosylated.
Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP31596.
PRIDEiP31596.

PTM databases

iPTMnetiP31596.
PhosphoSiteiP31596.
SwissPalmiP31596.
UniCarbKBiP31596.

Expressioni

Tissue specificityi

Localized in brain and is highly enriched in the Purkinje cell layer in cerebellum.

Interactioni

Subunit structurei

Homotrimer (By similarity). Interacts with AJUBA.By similarity1 Publication

Protein-protein interaction databases

BioGridi248124. 1 interaction.
IntActiP31596. 1 interaction.
STRINGi10116.ENSRNOP00000007604.

Structurei

3D structure databases

ProteinModelPortaliP31596.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP31596.
KOiK05613.
PhylomeDBiP31596.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 2 hits.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Glt1 (identifier: P31596-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTEGANNM PKQVEVRMHD SHLSSEEPKH RNLGMRMCDK LGKNLLLSLT
60 70 80 90 100
VFGVILGAVC GGLLRLAAPI HPDVVMLIAF PGDILMRMLK MLILPLIISS
110 120 130 140 150
LITGLSGLDA KASGRLGTRA MVYYMSTTII AAVLGVILVL AIHPGNPKLK
160 170 180 190 200
KQLGPGKKND EVSSLDAFLD LIRNLFPENL VQACFQQIQT VTKKVLVAPP
210 220 230 240 250
SEEANTTKAV ISLLNETMNE APEETKIVIK KGLEFKDGMN VLGLIGFFIA
260 270 280 290 300
FGIAMGKMGE QAKLMVEFFN ILNEIVMKLV IMIMWYSPLG IACLICGKII
310 320 330 340 350
AIKDLEVVAR QLGMYMITVI VGLIIHGGIF LPLIYFVVTR KNPFSFFAGI
360 370 380 390 400
FQAWITALGT ASSAGTLPVT FRCLEDNLGI DKRVTRFVLP VGATINMDGT
410 420 430 440 450
ALYEAVAAIF IAQMNGVILD GGQIVTVSLT ATLASIGAAS IPSAGLVTML
460 470 480 490 500
LILTAVGLPT EDISLLVAVD WLLDRMRTSV NVVGDSFGAG IVYHLSKSEL
510 520 530 540 550
DTIDSQHRMH EDIEMTKTQS VYDDTKNHRE SNSNQCVYAA HNSVVIDECK
560 570
VTLAANGKSA DCSVEEEPWK REK
Length:573
Mass (Da):62,106
Last modified:October 1, 1993 - v2
Checksum:i8C51D30954E00E7F
GO
Isoform Glt-1A (identifier: P31596-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MASTEG → MVS

Show »
Length:570
Mass (Da):61,847
Checksum:i8231169B6B0F2F1E
GO

Sequence cautioni

The sequence AAA93062.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti521 – 5211V → I in AAA93061 (Ref. 3) Curated
Sequence conflicti521 – 5211V → I in AAA93062 (Ref. 3) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 66MASTEG → MVS in isoform Glt-1A. 1 PublicationVSP_006266

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67857 mRNA. Translation: CAA48042.1. Sequence problems.
U15098 mRNA. Translation: AAA93061.1.
U15098 mRNA. Translation: AAA93062.1. Different initiation.
AF297648 mRNA. Translation: AAG13411.1.
PIRiS28901.
RefSeqiNP_001289018.1. NM_001302089.1.
NP_058911.2. NM_017215.2.
UniGeneiRn.10240.

Genome annotation databases

GeneIDi29482.
KEGGirno:29482.
UCSCiRGD:3697. rat. [P31596-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67857 mRNA. Translation: CAA48042.1. Sequence problems.
U15098 mRNA. Translation: AAA93061.1.
U15098 mRNA. Translation: AAA93062.1. Different initiation.
AF297648 mRNA. Translation: AAG13411.1.
PIRiS28901.
RefSeqiNP_001289018.1. NM_001302089.1.
NP_058911.2. NM_017215.2.
UniGeneiRn.10240.

3D structure databases

ProteinModelPortaliP31596.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248124. 1 interaction.
IntActiP31596. 1 interaction.
STRINGi10116.ENSRNOP00000007604.

Chemistry

ChEMBLiCHEMBL2900.
GuidetoPHARMACOLOGYi869.

Protein family/group databases

TCDBi2.A.23.2.2. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

PTM databases

iPTMnetiP31596.
PhosphoSiteiP31596.
SwissPalmiP31596.
UniCarbKBiP31596.

Proteomic databases

PaxDbiP31596.
PRIDEiP31596.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29482.
KEGGirno:29482.
UCSCiRGD:3697. rat. [P31596-1]

Organism-specific databases

CTDi6506.
RGDi3697. Slc1a2.

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP31596.
KOiK05613.
PhylomeDBiP31596.
TreeFamiTF315206.

Miscellaneous databases

PROiP31596.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 2 hits.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Glutamate transporters from brain. A novel neurotransmitter transporter family."
    Kanner B.I.
    FEBS Lett. 325:95-99(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 260-289.
  3. Roginski R.S., Choudhury K., Meiners S., Marone M., Basma A.N., Geller H.M.
    Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Forebrain.
  4. "The rat hepatoma cell line H4-II-E-C3 expresses high activities of the high-affinity glutamate transporter GLT-1A."
    Pollard M., McGivan J.
    FEBS Lett. 484:74-76(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GLT-1A).
    Tissue: Hepatoma.
  5. Lubec G., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 158-173, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  6. "Histidine 326 is critical for the function of GLT-1, a (Na+ + K+)-coupled glutamate transporter from rat brain."
    Zhang Y., Pines G., Kanner B.I.
    J. Biol. Chem. 269:19573-19577(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LYS-298 AND HIS-326.
  7. "The amino terminus of the glial glutamate transporter GLT-1 interacts with the LIM protein Ajuba."
    Marie H., Billups D., Bedford F.K., Dumoulin A., Goyal R.K., Longmore G.D., Moss S.J., Attwell D.
    Mol. Cell. Neurosci. 19:152-164(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AJUBA.
  8. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3; SER-21; SER-24 AND SER-25, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEAA2_RAT
AccessioniPrimary (citable) accession number: P31596
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 1, 1993
Last modified: July 6, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.