P31593 (GSA_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:

GSA

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. Porphyrin biosynthesis; chlorophyll biosynthesis.
Subunit structure	Homodimer.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast
	Chain	? – 478		Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic		PRO_0000001261
Amino acid modifications
	Modified residue	318	1	N6-(pyridoxal phosphate)lysine By similarity
Natural variations
	Natural variant	18	1	G → S in isozyme 2.
	Natural variant	104	1	E → D in isozyme 2.
	Natural variant	221	1	G → V in isozyme 2.
	Natural variant	227	1	S → T in isozyme 2.
	Natural variant	245	1	E → K in isozyme 2.
	Natural variant	270	1	L → P in isozyme 2.
	Natural variant	375	1	E → D in isozyme 2.
	Natural variant	411	1	L → F in isozyme 2.
	Natural variant	413	1	A → V in isozyme 2.
	Natural variant	466	1	K → R in isozyme 2.

Sequences

	Sequence		Length	Mass (Da)
	P31593 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 459CBA43FE46E84A	FASTA	478	50,877
	10 20 30 40 50 60 MAAVNGVGIS WPSKLTQGQR PKLVFSPSPR RCTPSSSTIK MTASVDEKKK TFTLQKSEEA 70 80 90 100 110 120 FSKAKELMPG GVNSPVRAFK SVGGQPIIID SVKGSRMRDI DGNEYIDYVG SWGPAIIGHA 130 140 150 160 170 180 DDEVLAALAE TMKKGTSFGA PCLLENTLAE MVISAVPSIE MVRFVNSGTE ACMGVLRLAR 190 200 210 220 230 240 AFTGRPKIIK FEGCYHGHAD PFLVKAGSGV ATLGLPDSPG GPKAATSDTL TAPYNDISAV 250 260 270 280 290 300 ESLFEEHKGE VAAIILEPVV GNAGFIQPNL DFLAAIRKIT KENDALLIFD EVMTGFRLAY 310 320 330 340 350 360 GGAQEYFGIT PDLTTLGKII GGGLPVGAYG GRRDIMEMVA PAGPMYQAGT LSGNPLAMTA 370 380 390 400 410 420 GIHTLKRLQG PGTYEYLDKI TGELTQGILD AGKKTGHAMC GGYIRGMFGF LFAEGPVNNF 430 440 450 460 470 SDAKKSDTEK FGRFYRGMLE EGVYFAPSQF EAGFTSLAHT SEDIQKTVAA AEKVLKQI

References

[1]

"A visible marker for antisense mRNA expression in plants: inhibition of chlorophyll synthesis with a glutamate-1-semialdehyde aminotransferase antisense gene." Hoefgen R., Axelsen K.B., Kannangara C.G., Schuettke I., Pohlenz H.-D., Willmitzer L., Grimm B., von Wettstein D. Proc. Natl. Acad. Sci. U.S.A. 91:1726-1730(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. SR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X65973 mRNA. Translation: CAA46786.1. X65974 mRNA. Translation: CAA46787.1.
PIR	S21454. S21455.
3D structure databases
ProteinModelPortal	P31593.
SMR	P31593. Positions 52-478.
ModBase	Search...
Proteomic databases
ProMEX	P31593.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00251; UER00317. UPA00668.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_TOBAC

Accession

Primary (citable) accession number: P31593

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program