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Protein

Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic

Gene

GSA

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-4-amino-5-oxopentanoate = 5-aminolevulinate.

Cofactori

Pathwayi

GO - Molecular functioni

  1. glutamate-1-semialdehyde 2,1-aminomutase activity Source: UniProtKB-EC
  2. pyridoxal phosphate binding Source: InterPro
  3. transaminase activity Source: InterPro

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Chlorophyll biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00251; UER00317.
UPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic (EC:5.4.3.8)
Short name:
GSA
Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name:
GSA-AT
Gene namesi
Name:GSA
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 478Glutamate-1-semialdehyde 2,1-aminomutase, chloroplasticPRO_0000001261
Transit peptidei1 – ?Chloroplast

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei318 – 3181N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

ProMEXiP31593.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP31593.
SMRiP31593. Positions 52-478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_00375. HemL_aminotrans_3.
InterProiIPR004639. 4pyrrol_synth_GluAld_NH2Trfase.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00713. hemL. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31593-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAVNGVGIS WPSKLTQGQR PKLVFSPSPR RCTPSSSTIK MTASVDEKKK
60 70 80 90 100
TFTLQKSEEA FSKAKELMPG GVNSPVRAFK SVGGQPIIID SVKGSRMRDI
110 120 130 140 150
DGNEYIDYVG SWGPAIIGHA DDEVLAALAE TMKKGTSFGA PCLLENTLAE
160 170 180 190 200
MVISAVPSIE MVRFVNSGTE ACMGVLRLAR AFTGRPKIIK FEGCYHGHAD
210 220 230 240 250
PFLVKAGSGV ATLGLPDSPG GPKAATSDTL TAPYNDISAV ESLFEEHKGE
260 270 280 290 300
VAAIILEPVV GNAGFIQPNL DFLAAIRKIT KENDALLIFD EVMTGFRLAY
310 320 330 340 350
GGAQEYFGIT PDLTTLGKII GGGLPVGAYG GRRDIMEMVA PAGPMYQAGT
360 370 380 390 400
LSGNPLAMTA GIHTLKRLQG PGTYEYLDKI TGELTQGILD AGKKTGHAMC
410 420 430 440 450
GGYIRGMFGF LFAEGPVNNF SDAKKSDTEK FGRFYRGMLE EGVYFAPSQF
460 470
EAGFTSLAHT SEDIQKTVAA AEKVLKQI
Length:478
Mass (Da):50,877
Last modified:July 1, 1993 - v1
Checksum:i459CBA43FE46E84A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti18 – 181G → S in isozyme 2.
Natural varianti104 – 1041E → D in isozyme 2.
Natural varianti221 – 2211G → V in isozyme 2.
Natural varianti227 – 2271S → T in isozyme 2.
Natural varianti245 – 2451E → K in isozyme 2.
Natural varianti270 – 2701L → P in isozyme 2.
Natural varianti375 – 3751E → D in isozyme 2.
Natural varianti411 – 4111L → F in isozyme 2.
Natural varianti413 – 4131A → V in isozyme 2.
Natural varianti466 – 4661K → R in isozyme 2.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65973 mRNA. Translation: CAA46786.1.
X65974 mRNA. Translation: CAA46787.1.
PIRiS21454.
S21455.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65973 mRNA. Translation: CAA46786.1.
X65974 mRNA. Translation: CAA46787.1.
PIRiS21454.
S21455.

3D structure databases

ProteinModelPortaliP31593.
SMRiP31593. Positions 52-478.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

ProMEXiP31593.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00251; UER00317.
UPA00668.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_00375. HemL_aminotrans_3.
InterProiIPR004639. 4pyrrol_synth_GluAld_NH2Trfase.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00713. hemL. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A visible marker for antisense mRNA expression in plants: inhibition of chlorophyll synthesis with a glutamate-1-semialdehyde aminotransferase antisense gene."
    Hoefgen R., Axelsen K.B., Kannangara C.G., Schuettke I., Pohlenz H.-D., Willmitzer L., Grimm B., von Wettstein D.
    Proc. Natl. Acad. Sci. U.S.A. 91:1726-1730(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. SR1.

Entry informationi

Entry nameiGSA_TOBAC
AccessioniPrimary (citable) accession number: P31593
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: January 7, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.