Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamine synthetase

Gene

glnT

Organism
Rhizobium leguminosarum bv. phaseoli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Miscellaneous

Two forms of glutamine synthetase (GSII and GSIII) can be found in this nitrogen fixing bacteria, GSII is a typical eukaryotic glutamine synthetase whereas GSIII is a divergent type with very low sequence similarity to the type I and II enzymes.Curated

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi123Magnesium 1By similarity1
Metal bindingi125Magnesium 2By similarity1
Metal bindingi180Magnesium 2By similarity1
Metal bindingi187Magnesium 2By similarity1
Binding sitei232L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi236Magnesium 1; via pros nitrogenBy similarity1
Binding sitei240ATPBy similarity1
Binding sitei291L-glutamateBy similarity1
Binding sitei315ATPBy similarity1
Binding sitei315L-glutamateBy similarity1
Binding sitei320ATPBy similarity1
Metal bindingi328Magnesium 1By similarity1
Binding sitei330L-glutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase1 Publication (EC:6.3.1.2By similarity)
Short name:
GS1 Publication
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase III1 Publication
Short name:
GSIIICurated
Gene namesi
Name:glnT1 Publication
OrganismiRhizobium leguminosarum bv. phaseoli
Taxonomic identifieri385 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532791 – 435Glutamine synthetaseAdd BLAST435

Interactioni

Subunit structurei

Homooctamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP31592.
SMRiP31592.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR017536. Glutamine_synthetase_typeIII.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR03105. gln_synth_III. 1 hit.
PROSITEiView protein in PROSITE
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P31592-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLDLAAFAR DKSIKYFMIS YTDLFGGQRA KLVPAEAIAD MQKDGAGFAG
60 70 80 90 100
FATWLDLTPA HPDLFAVPDA SSVIQLPWKK DVAWVAADCV MDDRPVEQAP
110 120 130 140 150
RVVLKRLVAE AAKEGLRVKT GVEPEFFLIS ADGSVISDQF DTAEKPCYDQ
160 170 180 190 200
QAVMRRYDVI AEICDYMLEL GWKPYQNDHE DANGQFEMNW EYDDVLKTAD
210 220 230 240 250
KHSFFKFMVK SVAEKHGLRA TFMPKPFKGL TGNGCHAHIS VWDVDGRVNA
260 270 280 290 300
FADKEMAFGL SAQGKTFLGG IMKHAPALAA ITNPTVNSYK RINAPRTTSG
310 320 330 340 350
ATWSPNTVTW TGNNRTHMVR VPGPGRFELR LPDGAVNPYL LQAIIIAAGL
360 370 380 390 400
EGIRSQADPG QHYDIDMYAE GHLVKDAPRL PLNLLDALRA FDADEGLKAA
410 420 430
IGAEFSSAYL KLKHLEWNAY CSHFTQWERD STLDI
Length:435
Mass (Da):48,270
Last modified:July 1, 1993 - v1
Checksum:i305FEAE2AC4A2B77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S48357 Genomic DNA. Translation: AAB23489.1.
PIRiJC1301.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S48357 Genomic DNA. Translation: AAB23489.1.
PIRiJC1301.

3D structure databases

ProteinModelPortaliP31592.
SMRiP31592.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR017536. Glutamine_synthetase_typeIII.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR03105. gln_synth_III. 1 hit.
PROSITEiView protein in PROSITE
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNA3_RHILP
AccessioniPrimary (citable) accession number: P31592
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 12, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.