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Protein

Ras-related protein RABF2a

Gene

RABF2A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the trafficking of soluble cargo proteins from the prevacuolar compartment to the central vacuole (PubMed:12724533, PubMed:21899678). Involved in vacuolar transport of storage proteins with EREX as effector. Regulates membrane trafficking to protein storage vacuoles (PSVs).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 25GTPBy similarity9
Nucleotide bindingi65 – 69GTPBy similarity5
Nucleotide bindingi123 – 126GTPBy similarity4
Nucleotide bindingi153 – 154GTPBy similarity2

GO - Molecular functioni

  • GTP binding Source: TAIR

GO - Biological processi

  • protein transport Source: UniProtKB-KW
  • small GTPase mediated signal transduction Source: InterPro
  • vacuolar transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein RABF2a
Short name:
AtRABF2a
Alternative name(s):
Ras-related protein Rab5A
Short name:
AtRab5A
Ras-related protein Rha1
Gene namesi
Name:RABF2A
Synonyms:RAB5A, RHA1
Ordered Locus Names:At5g45130
ORF Names:K17O22.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G45130.

Subcellular locationi

GO - Cellular componenti

  • endocytic vesicle Source: GO_Central
  • endoplasmic reticulum Source: TAIR
  • endosome Source: GO_Central
  • vacuolar membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi24S → N: Dominant negative (GDP-bound form); loss of targeting to the prevacuolar compartment. Inhibits vacuolar trafficking. No effect on the interaction with VPS9A. Loss of interaction with EREX. 5 Publications1
Mutagenesisi42T → A: Loss of targeting to the prevacuolar compartment, but no effect on the vacuolar trafficking. 2 Publications1
Mutagenesisi69Q → L: Constitutively active (GTP-bound form); no effect on the targeting to the prevacuolar compartment. Loss of interaction with VPS9A. 2 Publications1
Mutagenesisi123N → I: Blocks nucleotide binding; no effect on the interaction with VPS9A. 1 Publication1
Mutagenesisi198 – 199CC → SS: Loss of targeting to the prevacuolar compartment, but no effect on the vacuolar trafficking. 2 Publications2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001212771 – 200Ras-related protein RABF2aAdd BLAST200

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi198S-geranylgeranyl cysteineBy similarity1
Lipidationi199S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Prenylation

Proteomic databases

PaxDbiP31582.
PRIDEiP31582.

Expressioni

Tissue specificityi

High in stem, root, and inflorescence.

Developmental stagei

Expressed during pollen germination and pollen tube growth.1 Publication

Inductioni

Activated by VPS9A.1 Publication

Gene expression databases

GenevisibleiP31582. AT.

Interactioni

Subunit structurei

Interacts with VPS9A (PubMed:18055610). Interacts with EREX (via PX domain) (PubMed:27288222).2 Publications

Protein-protein interaction databases

BioGridi19798. 9 interactors.
IntActiP31582. 9 interactors.
STRINGi3702.AT5G45130.1.

Structurei

3D structure databases

ProteinModelPortaliP31582.
SMRiP31582.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi39 – 47Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0092. Eukaryota.
ENOG410YCCP. LUCA.
HOGENOMiHOG000233968.
InParanoidiP31582.
OMAiTSWHWSE.
OrthoDBiEOG09360MTI.
PhylomeDBiP31582.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31582-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSGNKNIN AKLVLLGDVG AGKSSLVLRF VKDQFVEFQE STIGAAFFSQ
60 70 80 90 100
TLAVNDATVK FEIWDTAGQE RYHSLAPMYY RGAAAAIIVF DITNQASFER
110 120 130 140 150
AKKWVQELQA QGNPNMVMAL AGNKADLLDA RKVSAEEAEI YAQENSLFFM
160 170 180 190 200
ETSAKTATNV KDIFYEIAKR LPRVQPAENP TGMVLPNGPG ATAVSSSCCA
Length:200
Mass (Da):21,654
Last modified:July 1, 1993 - v1
Checksum:i98E2ED559A59DE35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59152 mRNA. Translation: CAA41863.1.
Z22958 Genomic DNA. Translation: CAA80534.1.
AB019224 Genomic DNA. Translation: BAB09498.1.
CP002688 Genomic DNA. Translation: AED95208.1.
AF389298 mRNA. Translation: AAK63870.1.
AY097362 mRNA. Translation: AAM19878.1.
PIRiS23727.
RefSeqiNP_199326.1. NM_123881.4.
UniGeneiAt.23631.

Genome annotation databases

EnsemblPlantsiAT5G45130.1; AT5G45130.1; AT5G45130.
GeneIDi834549.
GrameneiAT5G45130.1; AT5G45130.1; AT5G45130.
KEGGiath:AT5G45130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59152 mRNA. Translation: CAA41863.1.
Z22958 Genomic DNA. Translation: CAA80534.1.
AB019224 Genomic DNA. Translation: BAB09498.1.
CP002688 Genomic DNA. Translation: AED95208.1.
AF389298 mRNA. Translation: AAK63870.1.
AY097362 mRNA. Translation: AAM19878.1.
PIRiS23727.
RefSeqiNP_199326.1. NM_123881.4.
UniGeneiAt.23631.

3D structure databases

ProteinModelPortaliP31582.
SMRiP31582.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19798. 9 interactors.
IntActiP31582. 9 interactors.
STRINGi3702.AT5G45130.1.

Proteomic databases

PaxDbiP31582.
PRIDEiP31582.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G45130.1; AT5G45130.1; AT5G45130.
GeneIDi834549.
GrameneiAT5G45130.1; AT5G45130.1; AT5G45130.
KEGGiath:AT5G45130.

Organism-specific databases

TAIRiAT5G45130.

Phylogenomic databases

eggNOGiKOG0092. Eukaryota.
ENOG410YCCP. LUCA.
HOGENOMiHOG000233968.
InParanoidiP31582.
OMAiTSWHWSE.
OrthoDBiEOG09360MTI.
PhylomeDBiP31582.

Enzyme and pathway databases

ReactomeiR-ATH-6798695. Neutrophil degranulation.
R-ATH-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

PROiP31582.

Gene expression databases

GenevisibleiP31582. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAF2A_ARATH
AccessioniPrimary (citable) accession number: P31582
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 30, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.