Reviewed, UniProtKB/Swiss-Prot P31581 (HCE2_ORYLA)

Names and origin

Protein names

Recommended name:
    High choriolytic enzyme 2
    EC=3.4.24.67
Alternative name(s):
    Hatching enzyme zinc-protease subunit HCE 2
    Choriolysin H 2
    HCE21

Gene names

Name:

hceb

Organism

Oryzias latipes (Medaka fish) (Japanese ricefish)

Taxonomic identifier

8090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Atherinomorpha › Beloniformes › Adrianichthyidae › Oryziinae › Oryzias

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Participates in the breakdown of the egg envelope, which is derived from the egg extracellular matrix, at the time of hatching. Thus allowing the newly hatched fish to swim free. HCE binds tightly to the egg envelope while it exerts the choriolytic swelling action.
Catalytic activity	Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-|-7-(4-methyl)coumarylamide.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Note: Stored as proenzymes in the zymogen granules.
Developmental stage	Production of the protein starts in day 2 to day 3 embryos and increases thereafter until hatching.
Post-translational modification	O-glycosylated Probable.
Miscellaneous	In medaka the hatching enzyme system is composed of two distinct proteases, the high choriolytic enzyme (HCE), of which there are two isoforms, and the low choriolytic enzyme (LCE).
Sequence similarities	Belongs to the peptidase M12A family.

Ontologies

Keywords
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Propeptide	21 – 79	59	Activation peptide		PRO_0000028948
	Chain	80 – 279	200	High choriolytic enzyme 2		PRO_0000028949
Sites
	Active site	179	1	By similarity
	Metal binding	178	1	Zinc; catalytic By similarity
	Metal binding	182	1	Zinc; catalytic By similarity
	Metal binding	188	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	62	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P31581-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 00244107A8B01B8C	FASTA	279	31,490
	10 20 30 40 50 60 MNLASSACLL LLFLLGIAQA LPVQNEEGHE EGNKEGHGEE GVEEGDEDDF VDFTTRILTS 70 80 90 100 110 120 NNNTDQLLLE GDLVAPTNRN AMKCWYNSCF WKKASNGFVV IPYVISSQYS RGEVATIEGA 130 140 150 160 170 180 MRAFNGRTCI RFVRRTNEYD FISVVSKNGC YSELGRKGGQ QELSLNRGGC MYSGIIQHEL 190 200 210 220 230 240 NHALGFQHEQ TRSDRDSYVR INWQNIIPAS AYNFNKHDTN NLNTPYDYSS IMHYGRDAFS 250 260 270 IAYGRDSITP IPNPNVPIGQ RNGMSRWDIT RSNVLYNCR

References

[1]

"Isolation of cDNAs for LCE and HCE, two constituent proteases of the hatching enzyme of Oryzias latipes, and concurrent expression of their mRNAs during development." Yasumasu S., Yamada K., Akasaka K., Mitsunaga K., Iuchi I., Shimada H., Yamagami K. Dev. Biol. 153:250-258(1992) [PubMed: 1397682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M96171 mRNA. Translation: AAA49439.1.
PIR	C48826.
RefSeq	NP_001098293.1.
UniGene	Ola.4722
3D structure databases
HSSP	HSSP built from PDB template 1IAE based on UniProtKB P07584.
ModBase	Search...
Protein family/group databases
MEROPS	M12.007.
Genome annotation databases
Ensembl	ENSORLG00000014863. Oryzias latipes. [Contig view]
GeneID	100049452.
Phylogenomic databases
HOVERGEN	P31581.
Family and domain databases
InterPro	IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR001506. Peptidase_M12A. [Graphical view]
Pfam	PF01400. Astacin. 1 hit. [Graphical view]
PRINTS	PR00480. ASTACIN.
SMART	SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HCE2_ORYLA

Accession

Primary (citable) accession number: P31581

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)