P31574 (FIXB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein fixB

Gene names

Name:	fixB
Synonyms:	yaaR
Ordered Locus Names:	b0042, JW0041

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for anaerobic carnitine reduction. May bring reductant to CaiA. Ref.5
Pathway	Amine and polyamine metabolism; carnitine metabolism. HAMAP MF_01056
Subunit structure	Heterodimer of fixA and fixB Probable.
Sequence similarities	Belongs to the ETF alpha-subunit/fixB family.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	FAD Flavoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carnitine metabolic process Inferred from mutant phenotype Ref.5. Source: EcoliWiki electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Protein fixB HAMAP MF_01056		PRO_0000167861
Regions
	Nucleotide binding	255 – 283	29	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P31574 [UniParc]. Last modified October 1, 1996. Version 3. Checksum: 443DBBD7F97EDE16	FASTA	313	33,513
	10 20 30 40 50 60 MNTFSQVWVF SDTPSRLPEL MNGAQALANQ INTFVLNDAD GAQAIQLGAN HVWKLNGKPD 70 80 90 100 110 120 DRMIEDYAGV MADTIRQHGA DGLVLLPNTR RGKLLAAKLG YRLKAAVSND ASTVSVQDGK 130 140 150 160 170 180 ATVKHMVYGG LAIGEERIAT PYAVLTISSG TFDAAQPDAS RTGETHTVEW QAPAVAITRT 190 200 210 220 230 240 ATQARQSNSV DLDKARLVVS VGRGIGSKEN IALAEQLCKA IGAELACSRP VAENEKWMEH 250 260 270 280 290 300 ERYVGISNLM LKPELYLAVG ISGQIQHMVG ANASQTIFAI NKDKNAPIFQ YADYGIVGDA 310 VKILPALTAA LAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The fix Escherichia coli region contains four genes related to carnitine metabolism." Eichler K., Buchet A., Bourgis F., Kleber H.-P., Mandrand-Berthelot M.-A. J. Basic Microbiol. 35:217-227(1995) [PubMed: 7473063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: O44:K74.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The fixA and fixB genes are necessary for anaerobic carnitine reduction in Escherichia coli." Walt A., Kahn M.L. J. Bacteriol. 184:4044-4047(2002) [PubMed: 12081978] [Abstract] Cited for: FUNCTION. Strain: K12 / BW25113.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71977 Genomic DNA. Translation: CAA50798.1. U00096 Genomic DNA. Translation: AAC73153.1. AP009048 Genomic DNA. Translation: BAE76037.1.
PIR	B64725.
RefSeq	NP_414584.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P31574.
SMR	P31574. Positions 4-313.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9621N.
IntAct	P31574. 8 interactions.
MINT	MINT-1240082.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000480; EBESCP00000000480; EBESCG00000000400. EBESCT00000014599; EBESCP00000013890; EBESCG00000013660.
GeneID	948939.
GenomeReviews	Gene locus JW0041 in contig AP009048_GR. Gene locus b0042 in contig U00096_GR.
KEGG	ecj:JW0041. eco:b0042.
PATRIC	32115183. VBIEscCol129921_0043.
Organism-specific databases
EchoBASE	EB1524.
EcoGene	EG11563. fixB.
Phylogenomic databases
eggNOG	COG2025.
GeneTree	EBGT00050000009611.
HOGENOM	HBG615986.
OMA	FSDIASR.
PhylomeDB	P31574.
ProtClustDB	PRK03363.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11563-MONOMER.
Gene expression databases
Genevestigator	P31574.
Family and domain databases
HAMAP	MF_01056. FixB. [Tree]
InterPro	IPR001308. ETF_a. IPR014730. ETF_a/b_N. IPR014731. ETF_asu_C. IPR018206. ETF_asu_C_CS. IPR023461. ETF_FixBsu_pred. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K03522.
Pfam	PF01012. ETF. 1 hit. PF00766. ETF_alpha. 1 hit. [Graphical view]
PIRSF	PIRSF000089. Electra_flavoP_a. 1 hit.
SMART	SM00893. ETF. 1 hit. [Graphical view]
PROSITE	PS00696. ETF_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FIXB_ECOLI

Accession

Primary (citable) accession number: P31574
Secondary accession number(s): Q2MCG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program