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P31574 (FIXB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein FixB
Gene names
Name:fixB
Synonyms:yaaR
Ordered Locus Names:b0042, JW0041
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for anaerobic carnitine reduction. May bring reductant to CaiA. Ref.5

Pathway

Amine and polyamine metabolism; carnitine metabolism. HAMAP-Rule MF_01056

Subunit structure

Heterodimer of FixA and FixB Probable.

Sequence similarities

Belongs to the ETF alpha-subunit/FixB family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

gssP0AES02EBI-554030,EBI-557080

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Protein FixB HAMAP-Rule MF_01056
PRO_0000167861

Regions

Nucleotide binding255 – 28329FAD Potential

Sequences

Sequence LengthMass (Da)Tools
P31574 [UniParc].

Last modified October 1, 1996. Version 3.
Checksum: 443DBBD7F97EDE16

FASTA31333,513
        10         20         30         40         50         60 
MNTFSQVWVF SDTPSRLPEL MNGAQALANQ INTFVLNDAD GAQAIQLGAN HVWKLNGKPD 

        70         80         90        100        110        120 
DRMIEDYAGV MADTIRQHGA DGLVLLPNTR RGKLLAAKLG YRLKAAVSND ASTVSVQDGK 

       130        140        150        160        170        180 
ATVKHMVYGG LAIGEERIAT PYAVLTISSG TFDAAQPDAS RTGETHTVEW QAPAVAITRT 

       190        200        210        220        230        240 
ATQARQSNSV DLDKARLVVS VGRGIGSKEN IALAEQLCKA IGAELACSRP VAENEKWMEH 

       250        260        270        280        290        300 
ERYVGISNLM LKPELYLAVG ISGQIQHMVG ANASQTIFAI NKDKNAPIFQ YADYGIVGDA 

       310 
VKILPALTAA LAR 

« Hide

References

« Hide 'large scale' references
[1]"The fix Escherichia coli region contains four genes related to carnitine metabolism."
Eichler K., Buchet A., Bourgis F., Kleber H.-P., Mandrand-Berthelot M.-A.
J. Basic Microbiol. 35:217-227(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: O44:K74.
[2]"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"The fixA and fixB genes are necessary for anaerobic carnitine reduction in Escherichia coli."
Walt A., Kahn M.L.
J. Bacteriol. 184:4044-4047(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: K12 / BW25113.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X71977 Genomic DNA. Translation: CAA50798.1.
U00096 Genomic DNA. Translation: AAC73153.1.
AP009048 Genomic DNA. Translation: BAE76037.1.
PIRB64725.
RefSeqNP_414584.1. NC_000913.3.
YP_488348.1. NC_007779.1.

3D structure databases

ProteinModelPortalP31574.
SMRP31574. Positions 2-313.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9621N.
IntActP31574. 12 interactions.
MINTMINT-1240082.
STRING511145.b0042.

Proteomic databases

PaxDbP31574.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73153; AAC73153; b0042.
BAE76037; BAE76037; BAE76037.
GeneID12933214.
948939.
KEGGecj:Y75_p0042.
eco:b0042.
PATRIC32115183. VBIEscCol129921_0043.

Organism-specific databases

EchoBASEEB1524.
EcoGeneEG11563. fixB.

Phylogenomic databases

eggNOGCOG2025.
HOGENOMHOG000247866.
KOK03522.
OMAPIAETEK.
OrthoDBEOG68WR5D.
PhylomeDBP31574.

Enzyme and pathway databases

BioCycEcoCyc:EG11563-MONOMER.
ECOL316407:JW0041-MONOMER.
UniPathwayUPA00117.

Gene expression databases

GenevestigatorP31574.

Family and domain databases

Gene3D3.40.50.1220. 1 hit.
3.40.50.620. 1 hit.
HAMAPMF_01056. FixB.
InterProIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR001308. ETF_a.
IPR014730. ETF_a/b_N.
IPR014731. ETF_asu_C.
IPR018206. ETF_asu_C_CS.
IPR023461. ETF_FixBsu.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF01012. ETF. 1 hit.
PF00766. ETF_alpha. 1 hit.
[Graphical view]
PIRSFPIRSF000089. Electra_flavoP_a. 1 hit.
SMARTSM00893. ETF. 1 hit.
[Graphical view]
SUPFAMSSF52467. SSF52467. 1 hit.
PROSITEPS00696. ETF_ALPHA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP31574.

Entry information

Entry nameFIXB_ECOLI
AccessionPrimary (citable) accession number: P31574
Secondary accession number(s): Q2MCG9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene