P31574 (FIXB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein FixB

Gene names

Name:	fixB
Synonyms:	yaaR
Ordered Locus Names:	b0042, JW0041

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for anaerobic carnitine reduction. May bring reductant to CaiA. Ref.5
Pathway	Amine and polyamine metabolism; carnitine metabolism. HAMAP-Rule MF_01056
Subunit structure	Heterodimer of FixA and FixB Probable.
Sequence similarities	Belongs to the ETF alpha-subunit/FixB family.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	FAD Flavoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carnitine metabolic process Inferred from mutant phenotype Ref.5. Source: EcoliWiki electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Protein FixB HAMAP-Rule MF_01056		PRO_0000167861
Regions
	Nucleotide binding	255 – 283	29	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P31574 [UniParc]. Last modified October 1, 1996. Version 3. Checksum: 443DBBD7F97EDE16	FASTA	313	33,513
	10 20 30 40 50 60 MNTFSQVWVF SDTPSRLPEL MNGAQALANQ INTFVLNDAD GAQAIQLGAN HVWKLNGKPD 70 80 90 100 110 120 DRMIEDYAGV MADTIRQHGA DGLVLLPNTR RGKLLAAKLG YRLKAAVSND ASTVSVQDGK 130 140 150 160 170 180 ATVKHMVYGG LAIGEERIAT PYAVLTISSG TFDAAQPDAS RTGETHTVEW QAPAVAITRT 190 200 210 220 230 240 ATQARQSNSV DLDKARLVVS VGRGIGSKEN IALAEQLCKA IGAELACSRP VAENEKWMEH 250 260 270 280 290 300 ERYVGISNLM LKPELYLAVG ISGQIQHMVG ANASQTIFAI NKDKNAPIFQ YADYGIVGDA 310 VKILPALTAA LAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The fix Escherichia coli region contains four genes related to carnitine metabolism." Eichler K., Buchet A., Bourgis F., Kleber H.-P., Mandrand-Berthelot M.-A. J. Basic Microbiol. 35:217-227(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: O44:K74.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The fixA and fixB genes are necessary for anaerobic carnitine reduction in Escherichia coli." Walt A., Kahn M.L. J. Bacteriol. 184:4044-4047(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: K12 / BW25113.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71977 Genomic DNA. Translation: CAA50798.1. U00096 Genomic DNA. Translation: AAC73153.1. AP009048 Genomic DNA. Translation: BAE76037.1.
PIR	B64725.
RefSeq	NP_414584.1. NC_000913.2. YP_488348.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P31574.
SMR	P31574. Positions 87-311.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9621N.
IntAct	P31574. 8 interactions.
MINT	MINT-1240082.
STRING	511145.b0042.
Proteomic databases
PaxDb	P31574.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73153; AAC73153; b0042. BAE76037; BAE76037; BAE76037.
GeneID	12933214. 948939.
KEGG	ecj:Y75_p0042. eco:b0042.
PATRIC	32115183. VBIEscCol129921_0043.
Organism-specific databases
EchoBASE	EB1524.
EcoGene	EG11563. fixB.
Phylogenomic databases
eggNOG	COG2025.
HOGENOM	HOG000247866.
KO	K03522.
OMA	FSDIASR.
ProtClustDB	PRK03363.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11563-MONOMER. ECOL316407:JW0041-MONOMER.
UniPathway	UPA00117.
Gene expression databases
Genevestigator	P31574.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_01056. FixB.
InterPro	IPR001308. ETF_a. IPR014730. ETF_a/b_N. IPR014731. ETF_asu_C. IPR018206. ETF_asu_C_CS. IPR023461. ETF_FixBsu. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Pfam	PF01012. ETF. 1 hit. PF00766. ETF_alpha. 1 hit. [Graphical view]
PIRSF	PIRSF000089. Electra_flavoP_a. 1 hit.
SMART	SM00893. ETF. 1 hit. [Graphical view]
PROSITE	PS00696. ETF_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FIXB_ECOLI

Accession

Primary (citable) accession number: P31574
Secondary accession number(s): Q2MCG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 118 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program