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P31574

- FIXB_ECOLI

UniProt

P31574 - FIXB_ECOLI

Protein

Protein FixB

Gene

fixB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 3 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Required for anaerobic carnitine reduction. May bring reductant to CaiA.1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi255 – 28329FADSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. electron carrier activity Source: InterPro
    2. flavin adenine dinucleotide binding Source: InterPro
    3. protein binding Source: IntAct

    GO - Biological processi

    1. carnitine metabolic process Source: EcoliWiki
    2. oxidation-reduction process Source: UniProtKB-HAMAP

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11563-MONOMER.
    ECOL316407:JW0041-MONOMER.
    UniPathwayiUPA00117.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein FixB
    Gene namesi
    Name:fixB
    Synonyms:yaaR
    Ordered Locus Names:b0042, JW0041
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11563. fixB.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Protein FixBPRO_0000167861Add
    BLAST

    Proteomic databases

    PaxDbiP31574.

    Expressioni

    Gene expression databases

    GenevestigatoriP31574.

    Interactioni

    Subunit structurei

    Heterodimer of FixA and FixB.Curated

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    gssP0AES02EBI-554030,EBI-557080

    Protein-protein interaction databases

    DIPiDIP-9621N.
    IntActiP31574. 12 interactions.
    MINTiMINT-1240082.
    STRINGi511145.b0042.

    Structurei

    3D structure databases

    ProteinModelPortaliP31574.
    SMRiP31574. Positions 2-313.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ETF alpha-subunit/FixB family.Curated

    Phylogenomic databases

    eggNOGiCOG2025.
    HOGENOMiHOG000247866.
    KOiK03522.
    OMAiPIAETEK.
    OrthoDBiEOG68WR5D.
    PhylomeDBiP31574.

    Family and domain databases

    Gene3Di3.40.50.1220. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPiMF_01056. FixB.
    InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR001308. ETF_a.
    IPR014730. ETF_a/b_N.
    IPR014731. ETF_asu_C.
    IPR018206. ETF_asu_C_CS.
    IPR023461. ETF_FixBsu.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF01012. ETF. 1 hit.
    PF00766. ETF_alpha. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000089. Electra_flavoP_a. 1 hit.
    SMARTiSM00893. ETF. 1 hit.
    [Graphical view]
    SUPFAMiSSF52467. SSF52467. 1 hit.
    PROSITEiPS00696. ETF_ALPHA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31574-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTFSQVWVF SDTPSRLPEL MNGAQALANQ INTFVLNDAD GAQAIQLGAN    50
    HVWKLNGKPD DRMIEDYAGV MADTIRQHGA DGLVLLPNTR RGKLLAAKLG 100
    YRLKAAVSND ASTVSVQDGK ATVKHMVYGG LAIGEERIAT PYAVLTISSG 150
    TFDAAQPDAS RTGETHTVEW QAPAVAITRT ATQARQSNSV DLDKARLVVS 200
    VGRGIGSKEN IALAEQLCKA IGAELACSRP VAENEKWMEH ERYVGISNLM 250
    LKPELYLAVG ISGQIQHMVG ANASQTIFAI NKDKNAPIFQ YADYGIVGDA 300
    VKILPALTAA LAR 313
    Length:313
    Mass (Da):33,513
    Last modified:October 1, 1996 - v3
    Checksum:i443DBBD7F97EDE16
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71977 Genomic DNA. Translation: CAA50798.1.
    U00096 Genomic DNA. Translation: AAC73153.1.
    AP009048 Genomic DNA. Translation: BAE76037.1.
    PIRiB64725.
    RefSeqiNP_414584.1. NC_000913.3.
    YP_488348.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73153; AAC73153; b0042.
    BAE76037; BAE76037; BAE76037.
    GeneIDi12933214.
    948939.
    KEGGiecj:Y75_p0042.
    eco:b0042.
    PATRICi32115183. VBIEscCol129921_0043.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71977 Genomic DNA. Translation: CAA50798.1 .
    U00096 Genomic DNA. Translation: AAC73153.1 .
    AP009048 Genomic DNA. Translation: BAE76037.1 .
    PIRi B64725.
    RefSeqi NP_414584.1. NC_000913.3.
    YP_488348.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P31574.
    SMRi P31574. Positions 2-313.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9621N.
    IntActi P31574. 12 interactions.
    MINTi MINT-1240082.
    STRINGi 511145.b0042.

    Proteomic databases

    PaxDbi P31574.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73153 ; AAC73153 ; b0042 .
    BAE76037 ; BAE76037 ; BAE76037 .
    GeneIDi 12933214.
    948939.
    KEGGi ecj:Y75_p0042.
    eco:b0042.
    PATRICi 32115183. VBIEscCol129921_0043.

    Organism-specific databases

    EchoBASEi EB1524.
    EcoGenei EG11563. fixB.

    Phylogenomic databases

    eggNOGi COG2025.
    HOGENOMi HOG000247866.
    KOi K03522.
    OMAi PIAETEK.
    OrthoDBi EOG68WR5D.
    PhylomeDBi P31574.

    Enzyme and pathway databases

    UniPathwayi UPA00117 .
    BioCyci EcoCyc:EG11563-MONOMER.
    ECOL316407:JW0041-MONOMER.

    Miscellaneous databases

    PROi P31574.

    Gene expression databases

    Genevestigatori P31574.

    Family and domain databases

    Gene3Di 3.40.50.1220. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPi MF_01056. FixB.
    InterProi IPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR001308. ETF_a.
    IPR014730. ETF_a/b_N.
    IPR014731. ETF_asu_C.
    IPR018206. ETF_asu_C_CS.
    IPR023461. ETF_FixBsu.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF01012. ETF. 1 hit.
    PF00766. ETF_alpha. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000089. Electra_flavoP_a. 1 hit.
    SMARTi SM00893. ETF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52467. SSF52467. 1 hit.
    PROSITEi PS00696. ETF_ALPHA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The fix Escherichia coli region contains four genes related to carnitine metabolism."
      Eichler K., Buchet A., Bourgis F., Kleber H.-P., Mandrand-Berthelot M.-A.
      J. Basic Microbiol. 35:217-227(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: O44:K74.
    2. "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
      Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
      Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "The fixA and fixB genes are necessary for anaerobic carnitine reduction in Escherichia coli."
      Walt A., Kahn M.L.
      J. Bacteriol. 184:4044-4047(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: K12 / BW25113.

    Entry informationi

    Entry nameiFIXB_ECOLI
    AccessioniPrimary (citable) accession number: P31574
    Secondary accession number(s): Q2MCG9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 129 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3