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Protein

L-carnitine CoA-transferase

Gene

caiB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transfer of the CoA moiety from gamma-butyrobetainyl-CoA to L-carnitine to generate L-carnitinyl-CoA and gamma-butyrobetaine. Is also able to catalyze the reversible transfer of the CoA moiety from gamma-butyrobetainyl-CoA or L-carnitinyl-CoA to crotonobetaine to generate crotonobetainyl-CoA.UniRule annotation1 Publication

Catalytic activityi

(E)-4-(trimethylammonio)but-2-enoyl-CoA + L-carnitine = (E)-4-(trimethylammonio)but-2-enoate + L-carnitinyl-CoA.UniRule annotation1 Publication
4-trimethylammoniobutanoyl-CoA + L-carnitine = 4-trimethylammoniobutanoate + L-carnitinyl-CoA.UniRule annotation1 Publication

Pathwayi: carnitine metabolism

This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei97Coenzyme AUniRule annotation1 Publication1
Binding sitei104Coenzyme AUniRule annotation1 Publication1
Active sitei169NucleophileUniRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciEcoCyc:CARNDEHYDRA-MONOMER.
ECOL316407:JW0037-MONOMER.
MetaCyc:CARNDEHYDRA-MONOMER.
BRENDAi2.8.3.21. 2026.
UniPathwayiUPA00117.

Names & Taxonomyi

Protein namesi
Recommended name:
L-carnitine CoA-transferaseUniRule annotation (EC:2.8.3.21UniRule annotation1 Publication)
Alternative name(s):
Crotonobetainyl-CoA:carnitine CoA-transferaseUniRule annotation
Gene namesi
Name:caiB1 PublicationUniRule annotation
Synonyms:yaaN
Ordered Locus Names:b0038, JW0037
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11559. caiB.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001947081 – 405L-carnitine CoA-transferaseAdd BLAST405

Proteomic databases

PaxDbiP31572.
PRIDEiP31572.

Expressioni

Inductioni

By L-carnitine or crotonobetaine.

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4261584. 5 interactors.
IntActiP31572. 12 interactors.
STRINGi511145.b0038.

Structurei

Secondary structure

1405
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni11 – 14Combined sources4
Beta strandi16 – 20Combined sources5
Helixi25 – 35Combined sources11
Beta strandi39 – 44Combined sources6
Beta strandi46 – 48Combined sources3
Helixi51 – 54Combined sources4
Beta strandi55 – 57Combined sources3
Helixi58 – 62Combined sources5
Turni63 – 65Combined sources3
Beta strandi67 – 71Combined sources5
Beta strandi73 – 75Combined sources3
Helixi76 – 86Combined sources11
Beta strandi90 – 95Combined sources6
Beta strandi97 – 99Combined sources3
Helixi100 – 103Combined sources4
Helixi108 – 114Combined sources7
Beta strandi119 – 126Combined sources8
Beta strandi128 – 130Combined sources3
Turni132 – 136Combined sources5
Helixi141 – 147Combined sources7
Helixi151 – 153Combined sources3
Beta strandi154 – 156Combined sources3
Turni164 – 166Combined sources3
Helixi167 – 190Combined sources24
Beta strandi194 – 199Combined sources6
Helixi200 – 207Combined sources8
Helixi209 – 216Combined sources8
Beta strandi231 – 233Combined sources3
Beta strandi236 – 241Combined sources6
Beta strandi244 – 249Combined sources6
Helixi253 – 263Combined sources11
Helixi266 – 268Combined sources3
Beta strandi271 – 274Combined sources4
Turni283 – 285Combined sources3
Helixi289 – 301Combined sources13
Helixi305 – 314Combined sources10
Beta strandi318 – 321Combined sources4
Helixi325 – 327Combined sources3
Helixi332 – 337Combined sources6
Beta strandi340 – 344Combined sources5
Beta strandi350 – 354Combined sources5
Beta strandi361 – 363Combined sources3
Turni374 – 377Combined sources4
Helixi378 – 384Combined sources7
Helixi389 – 397Combined sources9
Beta strandi400 – 402Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XA3X-ray1.85A/B2-405[»]
1XA4X-ray1.90A/B2-405[»]
1XK6X-ray1.85A/B/C/D1-405[»]
1XK7X-ray1.60A/B/C1-405[»]
1XVTX-ray2.30A1-405[»]
1XVUX-ray2.40A1-405[»]
1XVVX-ray2.40A1-405[»]
ProteinModelPortaliP31572.
SMRiP31572.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31572.

Family & Domainsi

Sequence similaritiesi

Belongs to the CaiB/BaiF CoA-transferase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105C04. Bacteria.
COG1804. LUCA.
HOGENOMiHOG000219745.
InParanoidiP31572.
KOiK08298.
OMAiVMPKFKN.
PhylomeDBiP31572.

Family and domain databases

Gene3Di3.40.50.10540. 2 hits.
HAMAPiMF_01050. CaiB. 1 hit.
InterProiIPR023452. CoA-Trfase_CaiB.
IPR003673. CoA-Trfase_fam_III.
IPR023606. CoA-Trfase_III_dom.
[Graphical view]
PfamiPF02515. CoA_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF89796. SSF89796. 1 hit.

Sequencei

Sequence statusi: Complete.

P31572-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHLPMPKFG PLAGLRVVFS GIEIAGPFAG QMFAEWGAEV IWIENVAWAD
60 70 80 90 100
TIRVQPNYPQ LSRRNLHALS LNIFKDEGRE AFLKLMETTD IFIEASKGPA
110 120 130 140 150
FARRGITDEV LWQHNPKLVI AHLSGFGQYG TEEYTNLPAY NTIAQAFSGY
160 170 180 190 200
LIQNGDVDQP MPAFPYTADY FSGLTATTAA LAALHKVRET GKGESIDIAM
210 220 230 240 250
YEVMLRMGQY FMMDYFNGGE MCPRMSKGKD PYYAGCGLYK CADGYIVMEL
260 270 280 290 300
VGITQIEECF KDIGLAHLLG TPEIPEGTQL IHRIECPYGP LVEEKLDAWL
310 320 330 340 350
ATHTIAEVKE RFAELNIACA KVLTVPELES NPQYVARESI TQWQTMDGRT
360 370 380 390 400
CKGPNIMPKF KNNPGQIWRG MPSHGMDTAA ILKNIGYSEN DIQELVSKGL

AKVED
Length:405
Mass (Da):45,127
Last modified:February 1, 1995 - v3
Checksum:i75583684B4B5A2DE
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti187V → A in strain: O44:K74. 1
Natural varianti302T → A in strain: O44:K74. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67748 Genomic DNA. Translation: CAA47971.1.
X73904 Genomic DNA. Translation: CAA52112.1.
U00096 Genomic DNA. Translation: AAC73149.1.
AP009048 Genomic DNA. Translation: BAB96607.1.
PIRiS40559.
RefSeqiNP_414580.1. NC_000913.3.
WP_000349936.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73149; AAC73149; b0038.
BAB96607; BAB96607; BAB96607.
GeneIDi948997.
KEGGiecj:JW0037.
eco:b0038.
PATRICi32115171. VBIEscCol129921_0037.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67748 Genomic DNA. Translation: CAA47971.1.
X73904 Genomic DNA. Translation: CAA52112.1.
U00096 Genomic DNA. Translation: AAC73149.1.
AP009048 Genomic DNA. Translation: BAB96607.1.
PIRiS40559.
RefSeqiNP_414580.1. NC_000913.3.
WP_000349936.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XA3X-ray1.85A/B2-405[»]
1XA4X-ray1.90A/B2-405[»]
1XK6X-ray1.85A/B/C/D1-405[»]
1XK7X-ray1.60A/B/C1-405[»]
1XVTX-ray2.30A1-405[»]
1XVUX-ray2.40A1-405[»]
1XVVX-ray2.40A1-405[»]
ProteinModelPortaliP31572.
SMRiP31572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261584. 5 interactors.
IntActiP31572. 12 interactors.
STRINGi511145.b0038.

Proteomic databases

PaxDbiP31572.
PRIDEiP31572.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73149; AAC73149; b0038.
BAB96607; BAB96607; BAB96607.
GeneIDi948997.
KEGGiecj:JW0037.
eco:b0038.
PATRICi32115171. VBIEscCol129921_0037.

Organism-specific databases

EchoBASEiEB1520.
EcoGeneiEG11559. caiB.

Phylogenomic databases

eggNOGiENOG4105C04. Bacteria.
COG1804. LUCA.
HOGENOMiHOG000219745.
InParanoidiP31572.
KOiK08298.
OMAiVMPKFKN.
PhylomeDBiP31572.

Enzyme and pathway databases

UniPathwayiUPA00117.
BioCyciEcoCyc:CARNDEHYDRA-MONOMER.
ECOL316407:JW0037-MONOMER.
MetaCyc:CARNDEHYDRA-MONOMER.
BRENDAi2.8.3.21. 2026.

Miscellaneous databases

EvolutionaryTraceiP31572.
PROiP31572.

Family and domain databases

Gene3Di3.40.50.10540. 2 hits.
HAMAPiMF_01050. CaiB. 1 hit.
InterProiIPR023452. CoA-Trfase_CaiB.
IPR003673. CoA-Trfase_fam_III.
IPR023606. CoA-Trfase_III_dom.
[Graphical view]
PfamiPF02515. CoA_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF89796. SSF89796. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCAIB_ECOLI
AccessioniPrimary (citable) accession number: P31572
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Some strains of E.coli, such as ATCC 25922, can metabolize carnitine under aerobiosis.1 Publication

Caution

Was originally thought to be an L-carnitine dehydratase.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.