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P31570 (BTUR_SALTY) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cob(I)yrinic acid a,c-diamide adenosyltransferase

EC=2.5.1.17
Alternative name(s):
Cob(I)alamin adenosyltransferase
Corrinoid adenosyltransferase
Gene names
Name:btuR
Synonyms:cobA
Ordered Locus Names:STM1718
OrganismSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]
Taxonomic identifier99287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

Protein attributes

Sequence length196 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for both de novo synthesis of the corrin ring for the assimilation of exogenous corrinoids. Participates in the adenosylation of a variety of incomplete and complete corrinoids.

Catalytic activity

ATP + cob(I)yrinic acid a,c-diamide = triphosphate + adenosylcob(III)yrinic acid a,c-diamide.

ATP + cobinamide = triphosphate + adenosylcobinamide.

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 2/7.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the Cob(I)alamin adenosyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 196196Cob(I)yrinic acid a,c-diamide adenosyltransferase
PRO_0000065010

Regions

Nucleotide binding36 – 427ATP By similarity

Secondary structure

................................. 196
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P31570 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: AE02E11176513CC4

FASTA19621,726
        10         20         30         40         50         60 
MSDERYQQRQ QKVKDRVDAR VAQAQEERGI IIVFTGNGKG KTTAAFGTAA RAVGHGKNVG 

        70         80         90        100        110        120 
VVQFIKGTWP NGERNLLEPH GVEFQVMATG FTWETQNREA DTAACMAVWQ HGKRMLADPL 

       130        140        150        160        170        180 
LDMVVLDELT YMVAYDYLPL EEVISALNAR PGHQTVIITG RGCHRDILDL ADTVSELRPV 

       190 
KHAFDAGVKA QMGIDY 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing and overexpression of cobA which encodes ATP:corrinoid adenosyltransferase in Salmonella typhimurium."
Suh S.-J., Escalante-Semerena J.C.
Gene 129:93-97(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: LT2.
[2]"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2."
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. expand/collapse author list , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.
Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LT2 / SGSC1412 / ATCC 700720.
[3]"Purification and initial characterization of the ATP:corrinoid adenosyltransferase encoded by the cobA gene of Salmonella typhimurium."
Suh S.-J., Escalante-Semerena J.C.
J. Bacteriol. 177:921-925(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-21, CHARACTERIZATION.
[4]"cobA function is required for both de novo cobalamin biosynthesis and assimilation of exogenous corrinoids in Salmonella typhimurium."
Escalante-Semerena J.C., Suh S.-J., Roth J.R.
J. Bacteriol. 172:273-280(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"The ATP:Co(I)rrinoid adenosyltransferase (CobA) enzyme of Salmonella enterica requires the 2'-OH group of ATP for function and yields inorganic triphosphate as its reaction byproduct."
Fonseca M.V., Buan N.R., Horswill A.R., Rayment I., Escalante-Semerena J.C.
J. Biol. Chem. 277:33127-33131(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Three-dimensional structure of ATP:corrinoid adenosyltransferase from Salmonella typhimurium in its free state, complexed with MgATP, or complexed with hydroxycobalamin and MgATP."
Bauer C.B., Fonseca M.V., Holden H.M., Thoden J.B., Thompson T.B., Escalante-Semerena J.C., Rayment I.
Biochemistry 40:361-374(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 27-183.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L08890 Unassigned DNA. Translation: AAA71929.1.
AE006468 Genomic DNA. Translation: AAL20636.1.
PIRJN0721.
RefSeqNP_460677.1. NC_003197.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1G5RX-ray2.10A1-196[»]
1G5TX-ray1.80A1-196[»]
1G64X-ray2.10A/B1-196[»]
4HUTX-ray1.95A/B6-196[»]
ProteinModelPortalP31570.
SMRP31570. Positions 7-196.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING99287.STM1718.

Proteomic databases

PaxDbP31570.
PRIDEP31570.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL20636; AAL20636; STM1718.
GeneID1253237.
KEGGstm:STM1718.
PATRIC32381951. VBISalEnt20916_1815.

Phylogenomic databases

eggNOGCOG2109.
HOGENOMHOG000260311.
KOK00798.
OMAPVKHAFE.
OrthoDBEOG6GN76J.
PhylomeDBP31570.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13220.
SENT99287:GCTI-1727-MONOMER.
UniPathwayUPA00148; UER00233.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003724. AdoCbl_synth_CblAdoTrfase_CobA.
IPR025826. Co_AT_N_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF12557. Co_AT_N. 1 hit.
PF02572. CobA_CobO_BtuR. 1 hit.
[Graphical view]
PIRSFPIRSF015617. Adensltrnsf_CobA. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00708. cobA. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP31570.

Entry information

Entry nameBTUR_SALTY
AccessionPrimary (citable) accession number: P31570
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways