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Protein

Cob(I)yrinic acid a,c-diamide adenosyltransferase

Gene

btuR

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for both de novo synthesis of the corrin ring for the assimilation of exogenous corrinoids. Participates in the adenosylation of a variety of incomplete and complete corrinoids.

Catalytic activityi

ATP + cob(I)yrinic acid a,c-diamide = triphosphate + adenosylcob(III)yrinic acid a,c-diamide.
ATP + cobinamide = triphosphate + adenosylcobinamide.

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Cob(I)yrinic acid a,c-diamide adenosyltransferase (btuR)
  3. no protein annotated in this organism
  4. Cobalamin biosynthesis protein CbiB (cbiB)
  5. Bifunctional adenosylcobalamin biosynthesis protein CobU (cobU)
  6. Bifunctional adenosylcobalamin biosynthesis protein CobU (cobU)
  7. Adenosylcobinamide-GDP ribazoletransferase (cobS)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 42ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13220.
BRENDAi2.5.1.17. 5542.
UniPathwayiUPA00148; UER00233.

Names & Taxonomyi

Protein namesi
Recommended name:
Cob(I)yrinic acid a,c-diamide adenosyltransferase (EC:2.5.1.17)
Alternative name(s):
Cob(I)alamin adenosyltransferase
Corrinoid adenosyltransferase
Gene namesi
Name:btuR
Synonyms:cobA
Ordered Locus Names:STM1718
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000650101 – 196Cob(I)yrinic acid a,c-diamide adenosyltransferaseAdd BLAST196

Proteomic databases

PaxDbiP31570.
PRIDEiP31570.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi99287.STM1718.

Structurei

Secondary structure

1196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 23Combined sources17
Beta strandi31 – 39Combined sources9
Helixi41 – 54Combined sources14
Beta strandi59 – 65Combined sources7
Helixi72 – 77Combined sources6
Helixi78 – 80Combined sources3
Beta strandi83 – 86Combined sources4
Helixi95 – 97Combined sources3
Helixi98 – 115Combined sources18
Beta strandi122 – 127Combined sources6
Helixi129 – 134Combined sources6
Helixi140 – 148Combined sources9
Beta strandi155 – 159Combined sources5
Helixi165 – 170Combined sources6
Beta strandi172 – 176Combined sources5
Helixi183 – 186Combined sources4
Turni192 – 194Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G5RX-ray2.10A1-196[»]
1G5TX-ray1.80A1-196[»]
1G64X-ray2.10A/B1-196[»]
4HUTX-ray1.95A/B6-196[»]
ProteinModelPortaliP31570.
SMRiP31570.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31570.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG41068B1. Bacteria.
COG2109. LUCA.
HOGENOMiHOG000260311.
KOiK19221.
OMAiHAMGEGF.
PhylomeDBiP31570.

Family and domain databases

CDDicd00561. CobA_CobO_BtuR. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR003724. CblAdoTrfase_CobA.
IPR025826. Co_AT_N_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF12557. Co_AT_N. 1 hit.
PF02572. CobA_CobO_BtuR. 1 hit.
[Graphical view]
PIRSFiPIRSF015617. Adensltrnsf_CobA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00708. cobA. 1 hit.

Sequencei

Sequence statusi: Complete.

P31570-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDERYQQRQ QKVKDRVDAR VAQAQEERGI IIVFTGNGKG KTTAAFGTAA
60 70 80 90 100
RAVGHGKNVG VVQFIKGTWP NGERNLLEPH GVEFQVMATG FTWETQNREA
110 120 130 140 150
DTAACMAVWQ HGKRMLADPL LDMVVLDELT YMVAYDYLPL EEVISALNAR
160 170 180 190
PGHQTVIITG RGCHRDILDL ADTVSELRPV KHAFDAGVKA QMGIDY
Length:196
Mass (Da):21,726
Last modified:July 1, 1993 - v1
Checksum:iAE02E11176513CC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08890 Unassigned DNA. Translation: AAA71929.1.
AE006468 Genomic DNA. Translation: AAL20636.1.
PIRiJN0721.
RefSeqiNP_460677.1. NC_003197.1.
WP_001278856.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20636; AAL20636; STM1718.
GeneIDi1253237.
KEGGistm:STM1718.
PATRICi32381951. VBISalEnt20916_1815.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08890 Unassigned DNA. Translation: AAA71929.1.
AE006468 Genomic DNA. Translation: AAL20636.1.
PIRiJN0721.
RefSeqiNP_460677.1. NC_003197.1.
WP_001278856.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G5RX-ray2.10A1-196[»]
1G5TX-ray1.80A1-196[»]
1G64X-ray2.10A/B1-196[»]
4HUTX-ray1.95A/B6-196[»]
ProteinModelPortaliP31570.
SMRiP31570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1718.

Proteomic databases

PaxDbiP31570.
PRIDEiP31570.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20636; AAL20636; STM1718.
GeneIDi1253237.
KEGGistm:STM1718.
PATRICi32381951. VBISalEnt20916_1815.

Phylogenomic databases

eggNOGiENOG41068B1. Bacteria.
COG2109. LUCA.
HOGENOMiHOG000260311.
KOiK19221.
OMAiHAMGEGF.
PhylomeDBiP31570.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00233.
BioCyciMetaCyc:MONOMER-13220.
BRENDAi2.5.1.17. 5542.

Miscellaneous databases

EvolutionaryTraceiP31570.

Family and domain databases

CDDicd00561. CobA_CobO_BtuR. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR003724. CblAdoTrfase_CobA.
IPR025826. Co_AT_N_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF12557. Co_AT_N. 1 hit.
PF02572. CobA_CobO_BtuR. 1 hit.
[Graphical view]
PIRSFiPIRSF015617. Adensltrnsf_CobA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00708. cobA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBTUR_SALTY
AccessioniPrimary (citable) accession number: P31570
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.