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P31570

- BTUR_SALTY

UniProt

P31570 - BTUR_SALTY

Protein

Cob(I)yrinic acid a,c-diamide adenosyltransferase

Gene

btuR

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Required for both de novo synthesis of the corrin ring for the assimilation of exogenous corrinoids. Participates in the adenosylation of a variety of incomplete and complete corrinoids.

    Catalytic activityi

    ATP + cob(I)yrinic acid a,c-diamide = triphosphate + adenosylcob(III)yrinic acid a,c-diamide.
    ATP + cobinamide = triphosphate + adenosylcobinamide.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi36 – 427ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cob(I)yrinic acid a,c-diamide adenosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
    2. porphyrin-containing compound biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Cobalamin biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13220.
    SENT99287:GCTI-1727-MONOMER.
    UniPathwayiUPA00148; UER00233.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cob(I)yrinic acid a,c-diamide adenosyltransferase (EC:2.5.1.17)
    Alternative name(s):
    Cob(I)alamin adenosyltransferase
    Corrinoid adenosyltransferase
    Gene namesi
    Name:btuR
    Synonyms:cobA
    Ordered Locus Names:STM1718
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 196196Cob(I)yrinic acid a,c-diamide adenosyltransferasePRO_0000065010Add
    BLAST

    Proteomic databases

    PaxDbiP31570.
    PRIDEiP31570.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi99287.STM1718.

    Structurei

    Secondary structure

    1
    196
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 2317
    Beta strandi31 – 399
    Helixi41 – 5414
    Beta strandi59 – 657
    Helixi72 – 776
    Helixi78 – 803
    Beta strandi83 – 864
    Helixi95 – 973
    Helixi98 – 11518
    Beta strandi122 – 1276
    Helixi129 – 1346
    Helixi140 – 1489
    Beta strandi155 – 1595
    Helixi165 – 1706
    Beta strandi172 – 1765
    Helixi183 – 1864
    Turni192 – 1943

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1G5RX-ray2.10A1-196[»]
    1G5TX-ray1.80A1-196[»]
    1G64X-ray2.10A/B1-196[»]
    4HUTX-ray1.95A/B6-196[»]
    ProteinModelPortaliP31570.
    SMRiP31570. Positions 7-196.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP31570.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2109.
    HOGENOMiHOG000260311.
    KOiK00798.
    OMAiPVKHAFE.
    OrthoDBiEOG6GN76J.
    PhylomeDBiP31570.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003724. AdoCbl_synth_CblAdoTrfase_CobA.
    IPR025826. Co_AT_N_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF12557. Co_AT_N. 1 hit.
    PF02572. CobA_CobO_BtuR. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015617. Adensltrnsf_CobA. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00708. cobA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P31570-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDERYQQRQ QKVKDRVDAR VAQAQEERGI IIVFTGNGKG KTTAAFGTAA    50
    RAVGHGKNVG VVQFIKGTWP NGERNLLEPH GVEFQVMATG FTWETQNREA 100
    DTAACMAVWQ HGKRMLADPL LDMVVLDELT YMVAYDYLPL EEVISALNAR 150
    PGHQTVIITG RGCHRDILDL ADTVSELRPV KHAFDAGVKA QMGIDY 196
    Length:196
    Mass (Da):21,726
    Last modified:July 1, 1993 - v1
    Checksum:iAE02E11176513CC4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08890 Unassigned DNA. Translation: AAA71929.1.
    AE006468 Genomic DNA. Translation: AAL20636.1.
    PIRiJN0721.
    RefSeqiNP_460677.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL20636; AAL20636; STM1718.
    GeneIDi1253237.
    KEGGistm:STM1718.
    PATRICi32381951. VBISalEnt20916_1815.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08890 Unassigned DNA. Translation: AAA71929.1 .
    AE006468 Genomic DNA. Translation: AAL20636.1 .
    PIRi JN0721.
    RefSeqi NP_460677.1. NC_003197.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1G5R X-ray 2.10 A 1-196 [» ]
    1G5T X-ray 1.80 A 1-196 [» ]
    1G64 X-ray 2.10 A/B 1-196 [» ]
    4HUT X-ray 1.95 A/B 6-196 [» ]
    ProteinModelPortali P31570.
    SMRi P31570. Positions 7-196.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM1718.

    Proteomic databases

    PaxDbi P31570.
    PRIDEi P31570.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL20636 ; AAL20636 ; STM1718 .
    GeneIDi 1253237.
    KEGGi stm:STM1718.
    PATRICi 32381951. VBISalEnt20916_1815.

    Phylogenomic databases

    eggNOGi COG2109.
    HOGENOMi HOG000260311.
    KOi K00798.
    OMAi PVKHAFE.
    OrthoDBi EOG6GN76J.
    PhylomeDBi P31570.

    Enzyme and pathway databases

    UniPathwayi UPA00148 ; UER00233 .
    BioCyci MetaCyc:MONOMER-13220.
    SENT99287:GCTI-1727-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P31570.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003724. AdoCbl_synth_CblAdoTrfase_CobA.
    IPR025826. Co_AT_N_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF12557. Co_AT_N. 1 hit.
    PF02572. CobA_CobO_BtuR. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015617. Adensltrnsf_CobA. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00708. cobA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing and overexpression of cobA which encodes ATP:corrinoid adenosyltransferase in Salmonella typhimurium."
      Suh S.-J., Escalante-Semerena J.C.
      Gene 129:93-97(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LT2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    3. "Purification and initial characterization of the ATP:corrinoid adenosyltransferase encoded by the cobA gene of Salmonella typhimurium."
      Suh S.-J., Escalante-Semerena J.C.
      J. Bacteriol. 177:921-925(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-21, CHARACTERIZATION.
    4. "cobA function is required for both de novo cobalamin biosynthesis and assimilation of exogenous corrinoids in Salmonella typhimurium."
      Escalante-Semerena J.C., Suh S.-J., Roth J.R.
      J. Bacteriol. 172:273-280(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "The ATP:Co(I)rrinoid adenosyltransferase (CobA) enzyme of Salmonella enterica requires the 2'-OH group of ATP for function and yields inorganic triphosphate as its reaction byproduct."
      Fonseca M.V., Buan N.R., Horswill A.R., Rayment I., Escalante-Semerena J.C.
      J. Biol. Chem. 277:33127-33131(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Three-dimensional structure of ATP:corrinoid adenosyltransferase from Salmonella typhimurium in its free state, complexed with MgATP, or complexed with hydroxycobalamin and MgATP."
      Bauer C.B., Fonseca M.V., Holden H.M., Thoden J.B., Thompson T.B., Escalante-Semerena J.C., Rayment I.
      Biochemistry 40:361-374(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 27-183.

    Entry informationi

    Entry nameiBTUR_SALTY
    AccessioniPrimary (citable) accession number: P31570
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3