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P31570

- BTUR_SALTY

UniProt

P31570 - BTUR_SALTY

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Protein

Cob(I)yrinic acid a,c-diamide adenosyltransferase

Gene
btuR, cobA, STM1718
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Required for both de novo synthesis of the corrin ring for the assimilation of exogenous corrinoids. Participates in the adenosylation of a variety of incomplete and complete corrinoids.

Catalytic activityi

ATP + cob(I)yrinic acid a,c-diamide = triphosphate + adenosylcob(III)yrinic acid a,c-diamide.
ATP + cobinamide = triphosphate + adenosylcobinamide.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 427ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cob(I)yrinic acid a,c-diamide adenosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
  2. porphyrin-containing compound biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13220.
SENT99287:GCTI-1727-MONOMER.
UniPathwayiUPA00148; UER00233.

Names & Taxonomyi

Protein namesi
Recommended name:
Cob(I)yrinic acid a,c-diamide adenosyltransferase (EC:2.5.1.17)
Alternative name(s):
Cob(I)alamin adenosyltransferase
Corrinoid adenosyltransferase
Gene namesi
Name:btuR
Synonyms:cobA
Ordered Locus Names:STM1718
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196Cob(I)yrinic acid a,c-diamide adenosyltransferasePRO_0000065010Add
BLAST

Proteomic databases

PaxDbiP31570.
PRIDEiP31570.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi99287.STM1718.

Structurei

Secondary structure

1
196
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 2317
Beta strandi31 – 399
Helixi41 – 5414
Beta strandi59 – 657
Helixi72 – 776
Helixi78 – 803
Beta strandi83 – 864
Helixi95 – 973
Helixi98 – 11518
Beta strandi122 – 1276
Helixi129 – 1346
Helixi140 – 1489
Beta strandi155 – 1595
Helixi165 – 1706
Beta strandi172 – 1765
Helixi183 – 1864
Turni192 – 1943

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G5RX-ray2.10A1-196[»]
1G5TX-ray1.80A1-196[»]
1G64X-ray2.10A/B1-196[»]
4HUTX-ray1.95A/B6-196[»]
ProteinModelPortaliP31570.
SMRiP31570. Positions 7-196.

Miscellaneous databases

EvolutionaryTraceiP31570.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2109.
HOGENOMiHOG000260311.
KOiK00798.
OMAiPVKHAFE.
OrthoDBiEOG6GN76J.
PhylomeDBiP31570.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003724. AdoCbl_synth_CblAdoTrfase_CobA.
IPR025826. Co_AT_N_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF12557. Co_AT_N. 1 hit.
PF02572. CobA_CobO_BtuR. 1 hit.
[Graphical view]
PIRSFiPIRSF015617. Adensltrnsf_CobA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00708. cobA. 1 hit.

Sequencei

Sequence statusi: Complete.

P31570-1 [UniParc]FASTAAdd to Basket

« Hide

MSDERYQQRQ QKVKDRVDAR VAQAQEERGI IIVFTGNGKG KTTAAFGTAA    50
RAVGHGKNVG VVQFIKGTWP NGERNLLEPH GVEFQVMATG FTWETQNREA 100
DTAACMAVWQ HGKRMLADPL LDMVVLDELT YMVAYDYLPL EEVISALNAR 150
PGHQTVIITG RGCHRDILDL ADTVSELRPV KHAFDAGVKA QMGIDY 196
Length:196
Mass (Da):21,726
Last modified:July 1, 1993 - v1
Checksum:iAE02E11176513CC4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L08890 Unassigned DNA. Translation: AAA71929.1.
AE006468 Genomic DNA. Translation: AAL20636.1.
PIRiJN0721.
RefSeqiNP_460677.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20636; AAL20636; STM1718.
GeneIDi1253237.
KEGGistm:STM1718.
PATRICi32381951. VBISalEnt20916_1815.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L08890 Unassigned DNA. Translation: AAA71929.1 .
AE006468 Genomic DNA. Translation: AAL20636.1 .
PIRi JN0721.
RefSeqi NP_460677.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G5R X-ray 2.10 A 1-196 [» ]
1G5T X-ray 1.80 A 1-196 [» ]
1G64 X-ray 2.10 A/B 1-196 [» ]
4HUT X-ray 1.95 A/B 6-196 [» ]
ProteinModelPortali P31570.
SMRi P31570. Positions 7-196.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM1718.

Proteomic databases

PaxDbi P31570.
PRIDEi P31570.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL20636 ; AAL20636 ; STM1718 .
GeneIDi 1253237.
KEGGi stm:STM1718.
PATRICi 32381951. VBISalEnt20916_1815.

Phylogenomic databases

eggNOGi COG2109.
HOGENOMi HOG000260311.
KOi K00798.
OMAi PVKHAFE.
OrthoDBi EOG6GN76J.
PhylomeDBi P31570.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00233 .
BioCyci MetaCyc:MONOMER-13220.
SENT99287:GCTI-1727-MONOMER.

Miscellaneous databases

EvolutionaryTracei P31570.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003724. AdoCbl_synth_CblAdoTrfase_CobA.
IPR025826. Co_AT_N_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF12557. Co_AT_N. 1 hit.
PF02572. CobA_CobO_BtuR. 1 hit.
[Graphical view ]
PIRSFi PIRSF015617. Adensltrnsf_CobA. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00708. cobA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing and overexpression of cobA which encodes ATP:corrinoid adenosyltransferase in Salmonella typhimurium."
    Suh S.-J., Escalante-Semerena J.C.
    Gene 129:93-97(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  3. "Purification and initial characterization of the ATP:corrinoid adenosyltransferase encoded by the cobA gene of Salmonella typhimurium."
    Suh S.-J., Escalante-Semerena J.C.
    J. Bacteriol. 177:921-925(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-21, CHARACTERIZATION.
  4. "cobA function is required for both de novo cobalamin biosynthesis and assimilation of exogenous corrinoids in Salmonella typhimurium."
    Escalante-Semerena J.C., Suh S.-J., Roth J.R.
    J. Bacteriol. 172:273-280(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "The ATP:Co(I)rrinoid adenosyltransferase (CobA) enzyme of Salmonella enterica requires the 2'-OH group of ATP for function and yields inorganic triphosphate as its reaction byproduct."
    Fonseca M.V., Buan N.R., Horswill A.R., Rayment I., Escalante-Semerena J.C.
    J. Biol. Chem. 277:33127-33131(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Three-dimensional structure of ATP:corrinoid adenosyltransferase from Salmonella typhimurium in its free state, complexed with MgATP, or complexed with hydroxycobalamin and MgATP."
    Bauer C.B., Fonseca M.V., Holden H.M., Thoden J.B., Thompson T.B., Escalante-Semerena J.C., Rayment I.
    Biochemistry 40:361-374(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 27-183.

Entry informationi

Entry nameiBTUR_SALTY
AccessioniPrimary (citable) accession number: P31570
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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