P31554 (LPTD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LPS-assembly protein lptD

Alternative name(s):
Organic solvent tolerance protein

Gene names

Name:	lptD
Synonyms:	imp, ostA, yabG
Ordered Locus Names:	b0054, JW0053

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	784 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Together with lptE, is involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis By similarity. Ref.12 Ref.13
Subunit structure	Forms a high-molecular-weight complex in the outer membrane. Interacts with lptE By similarity. Ref.11 Ref.12
Subcellular location	Cell outer membrane Ref.6 Ref.11.
Induction	Constitutively expressed. Ref.10
Post-translational modification	Forms at least two disulfide bridges, one inter- and one intramolecular. HAMAP MF_01411
Sequence similarities	Belongs to the lptD family.

Ontologies

Keywords
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	Gram-negative-bacterium-type cell outer membrane assembly Inferred from mutant phenotype Ref.12. Source: EcoCyc lipopolysaccharide transport Inferred from mutant phenotype Ref.12. Source: EcoliWiki response to organic substance Inferred from genetic interaction. Source: EcoCyc
Cellular component	cell outer membrane Inferred from direct assay Ref.9. Source: EcoCyc plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction Ref.12. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
lptE	P0ADC1	2	EBI-549369,EBI-1119442

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.5 Ref.6
	Chain	25 – 784	760	LPS-assembly protein lptD HAMAP MF_01411		PRO_0000020276

Sequences

	Sequence		Length	Mass (Da)
	P31554 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 906EA3FE8F8D3E1E	FASTA	784	89,671
	10 20 30 40 50 60 MKKRIPTLLA TMIATALYSQ QGLAADLASQ CMLGVPSYDR PLVQGDTNDL PVTINADHAK 70 80 90 100 110 120 GDYPDDAVFT GSVDIMQGNS RLQADEVQLH QKEAPGQPEP VRTVDALGNV HYDDNQVILK 130 140 150 160 170 180 GPKGWANLNT KDTNVWEGDY QMVGRQGRGK ADLMKQRGEN RYTILDNGSF TSCLPGSDTW 190 200 210 220 230 240 SVVGSEIIHD REEQVAEIWN ARFKVGPVPI FYSPYLQLPV GDKRRSGFLI PNAKYTTTNY 250 260 270 280 290 300 FEFYLPYYWN IAPNMDATIT PHYMHRRGNI MWENEFRYLS QAGAGLMELD YLPSDKVYED 310 320 330 340 350 360 EHPNDDSSRR WLFYWNHSGV MDQVWRFNVD YTKVSDPSYF NDFDNKYGSS TDGYATQKFS 370 380 390 400 410 420 VGYAVQNFNA TVSTKQFQVF SEQNTSSYSA EPQLDVNYYQ NDVGPFDTRI YGQAVHFVNT 430 440 450 460 470 480 RDDMPEATRV HLEPTINLPL SNNWGSINTE AKLLATHYQQ TNLDWYNSRN TTKLDESVNR 490 500 510 520 530 540 VMPQFKVDGK MVFERDMEML APGYTQTLEP RAQYLYVPYR DQSDIYNYDS SLLQSDYSGL 550 560 570 580 590 600 FRDRTYGGLD RIASANQVTT GVTSRIYDDA AVERFNISVG QIYYFTESRT GDDNITWEND 610 620 630 640 650 660 DKTGSLVWAG DTYWRISERW GLRGGIQYDT RLDNVATSNS SIEYRRDEDR LVQLNYRYAS 670 680 690 700 710 720 PEYIQATLPK YYSTAEQYKN GISQVGAVAS WPIADRWSIV GAYYYDTNAN KQADSMLGVQ 730 740 750 760 770 780 YSSCCYAIRV GYERKLNGWD NDKQHAVYDN AIGFNIELRG LSSNYGLGTQ EMLRSNILPY QNTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"N-hexane sensitivity of Escherichia coli due to low expression of ostA/imp by insertion of IS2 and identification of the gene product as an outer membrane protein." Abe S., Okutsu T., Negishi T., Nakajima H., Aono R. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-29. Strain: K12 / EMG2.
[6]	"n-Hexane sensitivity of Escherichia coli due to low expression of imp/ostA encoding an 87 kDa minor protein associated with the outer membrane." Abe S., Okutsu T., Nakajima H., Kakuda N., Ohtsu I., Aono R. Microbiology 149:1265-1273(2003) [PubMed: 12724388] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-32, SUBCELLULAR LOCATION. Strain: K12 / JA300 / ATCC 33588 / DSM 4776 / NCIMB 12220.
[7]	"Identification and characterization of a new gene of Escherichia coli K-12 involved in outer membrane permeability." Sampson B.A., Misra R., Benson S.A. Genetics 122:491-501(1989) [PubMed: 2547691] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Cloning of organic solvent tolerance gene ostA that determines n-hexane tolerance level in Escherichia coli." Aono R., Negishi T., Nakajima H. Appl. Environ. Microbiol. 60:4624-4626(1994) [PubMed: 7811102] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / JA300 / ATCC 33588 / DSM 4776 / NCIMB 12220.
[9]	"Imp/OstA is required for cell envelope biogenesis in Escherichia coli." Braun M., Silhavy T.J. Mol. Microbiol. 45:1289-1302(2002) [PubMed: 12207697] [Abstract] Cited for: CHARACTERIZATION.
[10]	"Transcriptional analysis of the ostA/imp gene involved in organic solvent sensitivity in Escherichia coli." Ohtsu I., Kakuda N., Tsukagoshi N., Dokyu N., Takagi H., Wachi M., Aono R. Biosci. Biotechnol. Biochem. 68:458-461(2004) [PubMed: 14981317] [Abstract] Cited for: INDUCTION.
[11]	"Protein complexes of the Escherichia coli cell envelope." Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O. J. Biol. Chem. 280:34409-34419(2005) [PubMed: 16079137] [Abstract] Cited for: SUBUNIT, SUBCELLULAR LOCATION. Strain: BL21-DE3.
[12]	"Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli." Wu T., McCandlish A.C., Gronenberg L.S., Chng S.-S., Silhavy T.J., Kahne D. Proc. Natl. Acad. Sci. U.S.A. 103:11754-11759(2006) [PubMed: 16861298] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH RLPB. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[13]	"Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli." Sperandeo P., Lau F.K., Carpentieri A., De Castro C., Molinaro A., Deho G., Silhavy T.J., Polissi A. J. Bacteriol. 190:4460-4469(2008) [PubMed: 18424520] [Abstract] Cited for: FUNCTION, GENE NAME. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB013134 Genomic DNA. Translation: BAA34130.1. U00096 Genomic DNA. Translation: AAC73165.1. AP009048 Genomic DNA. Translation: BAB96622.1.
PIR	F64726.
RefSeq	NP_414596.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P31554.
SMR	P31554. Positions 48-98.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10029N.
IntAct	P31554. 7 interactions.
MINT	MINT-1293333.
Protein family/group databases
TCDB	1.B.42.1.2. outer membrane lipopolysaccharide export porin (LPS-EP) family.
2D gel databases
SWISS-2DPAGE	P31554.
Proteomic databases
PRIDE	P31554.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001543; EBESCP00000001543; EBESCG00000001284. EBESCT00000001544; EBESCP00000001544; EBESCG00000001284. EBESCT00000001545; EBESCP00000001545; EBESCG00000001284. EBESCT00000015698; EBESCP00000014989; EBESCG00000014758.
GeneID	945011.
GenomeReviews	Gene locus JW0053 in contig AP009048_GR. Gene locus b0054 in contig U00096_GR.
KEGG	ecj:JW0053. eco:b0054.
PATRIC	32115207. VBIEscCol129921_0055.
Organism-specific databases
EchoBASE	EB1529.
EcoGene	EG11569. lptD.
Phylogenomic databases
eggNOG	COG1452.
GeneTree	EBGT00050000010300.
HOGENOM	HBG563511.
OMA	TPYYWNI.
PhylomeDB	P31554.
ProtClustDB	PRK03761.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11569-MONOMER.
Gene expression databases
Genevestigator	P31554.
Family and domain databases
HAMAP	MF_01411. OstA. [Tree]
InterPro	IPR020889. LipoPS_assembly_LptD. IPR005653. OstA-like_N. IPR007543. OstA_C. [Graphical view]
KO	K04744.
Pfam	PF03968. OstA. 1 hit. PF04453. OstA_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPTD_ECOLI

Accession

Primary (citable) accession number: P31554
Secondary accession number(s): P75631

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program