P31553 (CAIT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-carnitine/gamma-butyrobetaine antiporter

Gene names

Name:	caiT
Synonyms:	yaaP
Ordered Locus Names:	b0040, JW0039

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the exchange of L-carnitine for gamma-butyrobetaine and related betaines. HAMAP-Rule MF_01049
Pathway	Amine and polyamine metabolism; carnitine metabolism. HAMAP-Rule MF_01049
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.7.
Sequence similarities	Belongs to the BCCT transporter (TC 2.A.15) family. CaiT subfamily. [View classification]

Ontologies

Keywords
Biological process	Antiport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	(R)-carnitine transport Inferred from electronic annotation. Source: InterPro 4-(trimethylammonio)butanoate transport Inferred from electronic annotation. Source: InterPro carnitine metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.5. Source: EcoCyc
Molecular_function	(R)-carnitine:4-(trimethylammonio)butanoate antiporter activity Inferred from electronic annotation. Source: InterPro carnitine transmembrane transporter activity Inferred from direct assay Ref.5. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 504

504

L-carnitine/gamma-butyrobetaine antiporter HAMAP-Rule MF_01049

PRO_0000201486

Regions

Transmembrane

10 – 30

21

Helical; Potential

Transmembrane

51 – 71

21

Helical; Potential

Transmembrane

92 – 112

21

Helical; Potential

Transmembrane

143 – 163

21

Helical; Potential

Transmembrane

195 – 215

21

Helical; Potential

Transmembrane

231 – 251

21

Helical; Potential

Transmembrane

263 – 283

21

Helical; Potential

Transmembrane

316 – 336

21

Helical; Potential

Transmembrane

347 – 367

21

Helical; Potential

Transmembrane

398 – 418

21

Helical; Potential

Transmembrane

446 – 466

21

Helical; Potential

Transmembrane

475 – 495

21

Helical; Potential

Natural variations

Natural variant

353

1

L → M in strain: O44:K74.

Natural variant

374

1

I → M in strain: B.

Secondary structure

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504

Helix Strand Turn

Details...

Turn

13 – 15

3

Helix

16 – 27

12

Turn

28 – 31

4

Helix

33 – 71

39

Beta strand

72 – 74

3

Beta strand

79 – 82

4

Helix

88 – 96

9

Helix

103 – 117

15

Beta strand

120 – 122

3

Helix

128 – 141

14

Helix

144 – 162

19

Helix

172 – 175

4

Turn

177 – 179

3

Helix

188 – 219

32

Helix

229 – 247

19

Turn

249 – 251

3

Helix

255 – 301

47

Turn

306 – 308

3

Helix

312 – 315

4

Helix

317 – 326

10

Helix

328 – 337

10

Turn

339 – 341

3

Helix

344 – 375

32

Helix

382 – 385

4

Helix

386 – 388

3

Helix

390 – 399

10

Beta strand

401 – 403

3

Helix

406 – 432

27

Beta strand

437 – 441

5

Helix

447 – 466

20

Helix

470 – 500

31

Sequences

	Sequence		Length	Mass (Da)
	P31553 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: A852310DBEA22D82	FASTA	504	56,587
	10 20 30 40 50 60 MKNEKRKTGI EPKVFFPPLI IVGILCWLTV RDLDAANVVI NAVFSYVTNV WGWAFEWYMV 70 80 90 100 110 120 VMLFGWFWLV FGPYAKKRLG NEPPEFSTAS WIFMMFASCT SAAVLFWGSI EIYYYISTPP 130 140 150 160 170 180 FGLEPNSTGA KELGLAYSLF HWGPLPWATY SFLSVAFAYF FFVRKMEVIR PSSTLVPLVG 190 200 210 220 230 240 EKHAKGLFGT IVDNFYLVAL IFAMGTSLGL ATPLVTECMQ WLFGIPHTLQ LDAIIITCWI 250 260 270 280 290 300 ILNAICVACG LQKGVRIASD VRSYLSFLML GWVFIVSGAS FIMNYFTDSV GMLLMYLPRM 310 320 330 340 350 360 LFYTDPIAKG GFPQGWTVFY WAWWVIYAIQ MSIFLARISR GRTVRELCFG MVLGLTASTW 370 380 390 400 410 420 ILWTVLGSNT LLLIDKNIIN IPNLIEQYGV ARAIIETWAA LPLSTATMWG FFILCFIATV 430 440 450 460 470 480 TLVNACSYTL AMSTCREVRD GEEPPLLVRI GWSILVGIIG IVLLALGGLK PIQTAIIAGG 490 500 CPLFFVNIMV TLSFIKDAKQ NWKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli." Eichler K., Bourgis F., Buchet A., Kleber H.-P., Mandrand-Berthelot M.-A. Mol. Microbiol. 13:775-786(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: O44:K74.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 203. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"CaiT of Escherichia coli, a new transporter catalyzing L-carnitine/gamma-butyrobetaine exchange." Jung H., Buchholz M., Clausen J., Nietschke M., Revermann A., Schmid R., Jung K. J. Biol. Chem. 277:39251-39258(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12, CHARACTERIZATION.
[6]	"Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations." Lenski R.E., Winkworth C.L., Riley M.A. J. Mol. Evol. 56:498-508(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 233-428. Strain: B.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X73904 Genomic DNA. Translation: CAA52110.1.
U00096 Genomic DNA. Translation: AAC73151.1.
AP009048 Genomic DNA. Translation: BAB96609.2.
AY625104 Genomic DNA. Translation: AAT42458.1.

PIR

H64724.

RefSeq

NP_414582.1. NC_000913.2.
YP_488346.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WSX	X-ray	3.50	A/B/C	1-504	[»]
3HFX	X-ray	3.15	A	1-504	[»]

ProteinModelPortal

P31553.

SMR

P31553. Positions 12-504.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-9247N.

STRING

511145.b0040.

Protein family/group databases

TCDB

2.A.15.2.1. betaine/carnitine/choline transporter (BCCT) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73151; AAC73151; b0040.
BAB96609; BAB96609; BAB96609.

GeneID

12931772.
944765.

KEGG

ecj:Y75_p0040.
eco:b0040.

PATRIC

32115175. VBIEscCol129921_0039.

Organism-specific databases

EchoBASE

EB1522.

EcoGene

EG11561. caiT.

Phylogenomic databases

eggNOG

COG1292.

HOGENOM

HOG000053240.

KO

K05245.

OMA

WSVLVGV.

ProtClustDB

PRK03356.

Enzyme and pathway databases

BioCyc

EcoCyc:CAIT-MONOMER.
ECOL316407:JW0039-MONOMER.

UniPathway

UPA00117.

Gene expression databases

Genevestigator

P31553.

Family and domain databases

HAMAP

MF_01049. CaiT.

InterPro

IPR018093. BCCT_transporter_CS.
IPR000060. BCCT_transptr.
IPR023449. BCCT_transptr_CaiT.
[Graphical view]

Pfam

PF02028. BCCT. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00842. bcct. 1 hit.

PROSITE

PS01303. BCCT. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P31553.

Entry information

Entry name

CAIT_ECOLI

Accession

Primary (citable) accession number: P31553
Secondary accession number(s): P75624, Q6IU45

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program