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Protein

L-carnitine/gamma-butyrobetaine antiporter

Gene

caiT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the exchange of L-carnitine for gamma-butyrobetaine and related betaines.

Pathwayi: carnitine metabolism

This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.

GO - Molecular functioni

GO - Biological processi

  • 4-(trimethylammonio)butanoate transport Source: InterPro
  • carnitine metabolic process Source: UniProtKB-UniPathway
  • carnitine transmembrane transport Source: GOC
  • carnitine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CAIT-MONOMER.
ECOL316407:JW0039-MONOMER.
MetaCyc:CAIT-MONOMER.
UniPathwayiUPA00117.

Protein family/group databases

TCDBi2.A.15.2.1. the betaine/carnitine/choline transporter (bcct) family.

Names & Taxonomyi

Protein namesi
Recommended name:
L-carnitine/gamma-butyrobetaine antiporterUniRule annotation
Gene namesi
Name:caiTUniRule annotation
Synonyms:yaaP
Ordered Locus Names:b0040, JW0039
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11561. caiT.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3021HelicalUniRule annotationAdd
BLAST
Transmembranei51 – 7121HelicalUniRule annotationAdd
BLAST
Transmembranei92 – 11221HelicalUniRule annotationAdd
BLAST
Transmembranei143 – 16321HelicalUniRule annotationAdd
BLAST
Transmembranei195 – 21521HelicalUniRule annotationAdd
BLAST
Transmembranei231 – 25121HelicalUniRule annotationAdd
BLAST
Transmembranei263 – 28321HelicalUniRule annotationAdd
BLAST
Transmembranei316 – 33621HelicalUniRule annotationAdd
BLAST
Transmembranei347 – 36721HelicalUniRule annotationAdd
BLAST
Transmembranei398 – 41821HelicalUniRule annotationAdd
BLAST
Transmembranei446 – 46621HelicalUniRule annotationAdd
BLAST
Transmembranei475 – 49521HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504L-carnitine/gamma-butyrobetaine antiporterPRO_0000201486Add
BLAST

Proteomic databases

PaxDbiP31553.

Interactioni

Protein-protein interaction databases

BioGridi4260766. 345 interactions.
DIPiDIP-9247N.
STRINGi511145.b0040.

Structurei

Secondary structure

1
504
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni13 – 153Combined sources
Helixi16 – 2712Combined sources
Turni28 – 314Combined sources
Helixi33 – 7139Combined sources
Beta strandi72 – 743Combined sources
Beta strandi79 – 824Combined sources
Helixi88 – 969Combined sources
Helixi103 – 11715Combined sources
Beta strandi120 – 1223Combined sources
Helixi128 – 14114Combined sources
Helixi144 – 16219Combined sources
Helixi172 – 1754Combined sources
Turni177 – 1793Combined sources
Helixi188 – 21932Combined sources
Helixi229 – 24719Combined sources
Turni249 – 2513Combined sources
Helixi255 – 30147Combined sources
Turni306 – 3083Combined sources
Helixi312 – 3154Combined sources
Helixi317 – 32610Combined sources
Helixi328 – 33710Combined sources
Turni339 – 3413Combined sources
Helixi344 – 37532Combined sources
Helixi382 – 3854Combined sources
Helixi386 – 3883Combined sources
Helixi390 – 39910Combined sources
Beta strandi401 – 4033Combined sources
Helixi406 – 43227Combined sources
Beta strandi437 – 4415Combined sources
Helixi447 – 46620Combined sources
Helixi470 – 50031Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WSXX-ray3.50A/B/C1-504[»]
3HFXX-ray3.15A1-504[»]
ProteinModelPortaliP31553.
SMRiP31553. Positions 12-504.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31553.

Family & Domainsi

Sequence similaritiesi

Belongs to the BCCT transporter (TC 2.A.15) family. CaiT subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C94. Bacteria.
COG1292. LUCA.
HOGENOMiHOG000053240.
InParanoidiP31553.
KOiK05245.
OMAiKSLLGTM.
OrthoDBiEOG6DZDVN.
PhylomeDBiP31553.

Family and domain databases

HAMAPiMF_01049. CaiT.
InterProiIPR018093. BCCT_CS.
IPR000060. BCCT_transptr.
IPR023449. BCCT_transptr_CaiT.
[Graphical view]
PfamiPF02028. BCCT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00842. bcct. 1 hit.
PROSITEiPS01303. BCCT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31553-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNEKRKTGI EPKVFFPPLI IVGILCWLTV RDLDAANVVI NAVFSYVTNV
60 70 80 90 100
WGWAFEWYMV VMLFGWFWLV FGPYAKKRLG NEPPEFSTAS WIFMMFASCT
110 120 130 140 150
SAAVLFWGSI EIYYYISTPP FGLEPNSTGA KELGLAYSLF HWGPLPWATY
160 170 180 190 200
SFLSVAFAYF FFVRKMEVIR PSSTLVPLVG EKHAKGLFGT IVDNFYLVAL
210 220 230 240 250
IFAMGTSLGL ATPLVTECMQ WLFGIPHTLQ LDAIIITCWI ILNAICVACG
260 270 280 290 300
LQKGVRIASD VRSYLSFLML GWVFIVSGAS FIMNYFTDSV GMLLMYLPRM
310 320 330 340 350
LFYTDPIAKG GFPQGWTVFY WAWWVIYAIQ MSIFLARISR GRTVRELCFG
360 370 380 390 400
MVLGLTASTW ILWTVLGSNT LLLIDKNIIN IPNLIEQYGV ARAIIETWAA
410 420 430 440 450
LPLSTATMWG FFILCFIATV TLVNACSYTL AMSTCREVRD GEEPPLLVRI
460 470 480 490 500
GWSILVGIIG IVLLALGGLK PIQTAIIAGG CPLFFVNIMV TLSFIKDAKQ

NWKD
Length:504
Mass (Da):56,587
Last modified:November 1, 1997 - v2
Checksum:iA852310DBEA22D82
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti353 – 3531L → M in strain: O44:K74.
Natural varianti374 – 3741I → M in strain: B.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73904 Genomic DNA. Translation: CAA52110.1.
U00096 Genomic DNA. Translation: AAC73151.1.
AP009048 Genomic DNA. Translation: BAB96609.2.
AY625104 Genomic DNA. Translation: AAT42458.1.
PIRiH64724.
RefSeqiNP_414582.1. NC_000913.3.
WP_000787103.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73151; AAC73151; b0040.
BAB96609; BAB96609; BAB96609.
GeneIDi944765.
KEGGiecj:JW0039.
eco:b0040.
PATRICi32115175. VBIEscCol129921_0039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73904 Genomic DNA. Translation: CAA52110.1.
U00096 Genomic DNA. Translation: AAC73151.1.
AP009048 Genomic DNA. Translation: BAB96609.2.
AY625104 Genomic DNA. Translation: AAT42458.1.
PIRiH64724.
RefSeqiNP_414582.1. NC_000913.3.
WP_000787103.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WSXX-ray3.50A/B/C1-504[»]
3HFXX-ray3.15A1-504[»]
ProteinModelPortaliP31553.
SMRiP31553. Positions 12-504.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260766. 345 interactions.
DIPiDIP-9247N.
STRINGi511145.b0040.

Protein family/group databases

TCDBi2.A.15.2.1. the betaine/carnitine/choline transporter (bcct) family.

Proteomic databases

PaxDbiP31553.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73151; AAC73151; b0040.
BAB96609; BAB96609; BAB96609.
GeneIDi944765.
KEGGiecj:JW0039.
eco:b0040.
PATRICi32115175. VBIEscCol129921_0039.

Organism-specific databases

EchoBASEiEB1522.
EcoGeneiEG11561. caiT.

Phylogenomic databases

eggNOGiENOG4105C94. Bacteria.
COG1292. LUCA.
HOGENOMiHOG000053240.
InParanoidiP31553.
KOiK05245.
OMAiKSLLGTM.
OrthoDBiEOG6DZDVN.
PhylomeDBiP31553.

Enzyme and pathway databases

UniPathwayiUPA00117.
BioCyciEcoCyc:CAIT-MONOMER.
ECOL316407:JW0039-MONOMER.
MetaCyc:CAIT-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP31553.
PROiP31553.

Family and domain databases

HAMAPiMF_01049. CaiT.
InterProiIPR018093. BCCT_CS.
IPR000060. BCCT_transptr.
IPR023449. BCCT_transptr_CaiT.
[Graphical view]
PfamiPF02028. BCCT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00842. bcct. 1 hit.
PROSITEiPS01303. BCCT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli."
    Eichler K., Bourgis F., Buchet A., Kleber H.-P., Mandrand-Berthelot M.-A.
    Mol. Microbiol. 13:775-786(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: O44:K74.
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
    Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
    Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 203.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "CaiT of Escherichia coli, a new transporter catalyzing L-carnitine/gamma-butyrobetaine exchange."
    Jung H., Buchholz M., Clausen J., Nietschke M., Revermann A., Schmid R., Jung K.
    J. Biol. Chem. 277:39251-39258(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-12, CHARACTERIZATION.
  6. "Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations."
    Lenski R.E., Winkworth C.L., Riley M.A.
    J. Mol. Evol. 56:498-508(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 233-428.
    Strain: B.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiCAIT_ECOLI
AccessioniPrimary (citable) accession number: P31553
Secondary accession number(s): P75624, Q6IU45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: January 20, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.