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P31553 (CAIT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
L-carnitine/gamma-butyrobetaine antiporter
Gene names
Name:caiT
Synonyms:yaaP
Ordered Locus Names:b0040, JW0039
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the exchange of L-carnitine for gamma-butyrobetaine and related betaines. HAMAP MF_01049

Pathway

Amine and polyamine metabolism; carnitine metabolism. HAMAP MF_01049

Subcellular location

Cell inner membrane; Multi-pass membrane protein Ref.7.

Sequence similarities

Belongs to the BCCT transporter (TC 2.A.15) family. CaiT subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 504504L-carnitine/gamma-butyrobetaine antiporter HAMAP MF_01049
PRO_0000201486

Regions

Transmembrane10 – 3021Helical; Potential
Transmembrane51 – 7121Helical; Potential
Transmembrane92 – 11221Helical; Potential
Transmembrane143 – 16321Helical; Potential
Transmembrane195 – 21521Helical; Potential
Transmembrane231 – 25121Helical; Potential
Transmembrane263 – 28321Helical; Potential
Transmembrane316 – 33621Helical; Potential
Transmembrane347 – 36721Helical; Potential
Transmembrane398 – 41821Helical; Potential
Transmembrane446 – 46621Helical; Potential
Transmembrane475 – 49521Helical; Potential

Natural variations

Natural variant3531L → M in strain: O44:K74.
Natural variant3741I → M in strain: B.

Secondary structure

1 ................................................................... 504
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P31553 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: A852310DBEA22D82

FASTA50456,587
        10         20         30         40         50         60 
MKNEKRKTGI EPKVFFPPLI IVGILCWLTV RDLDAANVVI NAVFSYVTNV WGWAFEWYMV 

        70         80         90        100        110        120 
VMLFGWFWLV FGPYAKKRLG NEPPEFSTAS WIFMMFASCT SAAVLFWGSI EIYYYISTPP 

       130        140        150        160        170        180 
FGLEPNSTGA KELGLAYSLF HWGPLPWATY SFLSVAFAYF FFVRKMEVIR PSSTLVPLVG 

       190        200        210        220        230        240 
EKHAKGLFGT IVDNFYLVAL IFAMGTSLGL ATPLVTECMQ WLFGIPHTLQ LDAIIITCWI 

       250        260        270        280        290        300 
ILNAICVACG LQKGVRIASD VRSYLSFLML GWVFIVSGAS FIMNYFTDSV GMLLMYLPRM 

       310        320        330        340        350        360 
LFYTDPIAKG GFPQGWTVFY WAWWVIYAIQ MSIFLARISR GRTVRELCFG MVLGLTASTW 

       370        380        390        400        410        420 
ILWTVLGSNT LLLIDKNIIN IPNLIEQYGV ARAIIETWAA LPLSTATMWG FFILCFIATV 

       430        440        450        460        470        480 
TLVNACSYTL AMSTCREVRD GEEPPLLVRI GWSILVGIIG IVLLALGGLK PIQTAIIAGG 

       490        500 
CPLFFVNIMV TLSFIKDAKQ NWKD

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli."
Eichler K., Bourgis F., Buchet A., Kleber H.-P., Mandrand-Berthelot M.-A.
Mol. Microbiol. 13:775-786(1994) [PubMed: 7815937] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
Strain: O44:K74.
[2]"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 203.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"CaiT of Escherichia coli, a new transporter catalyzing L-carnitine/gamma-butyrobetaine exchange."
Jung H., Buchholz M., Clausen J., Nietschke M., Revermann A., Schmid R., Jung K.
J. Biol. Chem. 277:39251-39258(2002) [PubMed: 12163501] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-12, CHARACTERIZATION.
[6]"Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations."
Lenski R.E., Winkworth C.L., Riley M.A.
J. Mol. Evol. 56:498-508(2003) [PubMed: 12664169] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 233-428.
Strain: B.
[7]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X73904 Genomic DNA. Translation: CAA52110.1.
U00096 Genomic DNA. Translation: AAC73151.1.
AP009048 Genomic DNA. Translation: BAB96609.2.
AY625104 Genomic DNA. Translation: AAT42458.1.
PIRH64724.
RefSeqNP_414582.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2WSXX-ray3.50A/B/C1-504[»]
3HFXX-ray3.15A1-504[»]
ProteinModelPortalP31553.
SMRP31553. Positions 12-504.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-9247N.

Protein family/group databases

TCDB2.A.15.2.1. betaine/carnitine/choline transporter (BCCT) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000000646; EBESCP00000000646; EBESCG00000000539.
EBESCT00000017832; EBESCP00000017123; EBESCG00000016888.
GeneID944765.
GenomeReviewsGene locus JW0039 in contig AP009048_GR.
Gene locus b0040 in contig U00096_GR.
KEGGecj:JW0039.
eco:b0040.
PATRIC32115175. VBIEscCol129921_0039.

Organism-specific databases

EchoBASEEB1522.
EcoGeneEG11561. caiT.

Phylogenomic databases

eggNOGCOG1292.
GeneTreeEBGT00050000009329.
HOGENOMHBG682943.
OMAPLVTECI.
PhylomeDBP31553.
ProtClustDBPRK03356.

Enzyme and pathway databases

BioCycEcoCyc:CAIT-MONOMER.

Gene expression databases

GenevestigatorP31553.

Family and domain databases

HAMAPMF_01049. CaiT.
[Tree]
InterProIPR018093. BCCT_transporter_CS.
IPR000060. BCCT_transptr.
IPR023449. BCCT_transptr_CaiT.
[Graphical view]
KOK05245.
PfamPF02028. BCCT. 1 hit.
[Graphical view]
TIGRFAMsTIGR00842. Bcct. 1 hit.
PROSITEPS01303. BCCT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCAIT_ECOLI
AccessionPrimary (citable) accession number: P31553
Secondary accession number(s): P75624, Q6IU45
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: January 25, 2012
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents