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Protein

L-carnitine/gamma-butyrobetaine antiporter

Gene

caiT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the exchange of L-carnitine for gamma-butyrobetaine and related betaines.

Pathwayi: carnitine metabolism

This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CAIT-MONOMER.
ECOL316407:JW0039-MONOMER.
MetaCyc:CAIT-MONOMER.
UniPathwayiUPA00117.

Protein family/group databases

TCDBi2.A.15.2.1. the betaine/carnitine/choline transporter (bcct) family.

Names & Taxonomyi

Protein namesi
Recommended name:
L-carnitine/gamma-butyrobetaine antiporterUniRule annotation
Gene namesi
Name:caiTUniRule annotation
Synonyms:yaaP
Ordered Locus Names:b0040, JW0039
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11561. caiT.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei10 – 30HelicalUniRule annotationAdd BLAST21
Transmembranei51 – 71HelicalUniRule annotationAdd BLAST21
Transmembranei92 – 112HelicalUniRule annotationAdd BLAST21
Transmembranei143 – 163HelicalUniRule annotationAdd BLAST21
Transmembranei195 – 215HelicalUniRule annotationAdd BLAST21
Transmembranei231 – 251HelicalUniRule annotationAdd BLAST21
Transmembranei263 – 283HelicalUniRule annotationAdd BLAST21
Transmembranei316 – 336HelicalUniRule annotationAdd BLAST21
Transmembranei347 – 367HelicalUniRule annotationAdd BLAST21
Transmembranei398 – 418HelicalUniRule annotationAdd BLAST21
Transmembranei446 – 466HelicalUniRule annotationAdd BLAST21
Transmembranei475 – 495HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002014861 – 504L-carnitine/gamma-butyrobetaine antiporterAdd BLAST504

Proteomic databases

PaxDbiP31553.
PRIDEiP31553.

Interactioni

Protein-protein interaction databases

BioGridi4260766. 345 interactors.
DIPiDIP-9247N.
STRINGi511145.b0040.

Structurei

Secondary structure

1504
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni13 – 15Combined sources3
Helixi16 – 27Combined sources12
Turni28 – 31Combined sources4
Helixi33 – 71Combined sources39
Beta strandi72 – 74Combined sources3
Beta strandi79 – 82Combined sources4
Helixi88 – 96Combined sources9
Helixi103 – 117Combined sources15
Beta strandi120 – 122Combined sources3
Helixi128 – 141Combined sources14
Helixi144 – 162Combined sources19
Helixi172 – 175Combined sources4
Turni177 – 179Combined sources3
Helixi188 – 219Combined sources32
Helixi229 – 247Combined sources19
Turni249 – 251Combined sources3
Helixi255 – 301Combined sources47
Turni306 – 308Combined sources3
Helixi312 – 315Combined sources4
Helixi317 – 326Combined sources10
Helixi328 – 337Combined sources10
Turni339 – 341Combined sources3
Helixi344 – 375Combined sources32
Helixi382 – 385Combined sources4
Helixi386 – 388Combined sources3
Helixi390 – 399Combined sources10
Beta strandi401 – 403Combined sources3
Helixi406 – 432Combined sources27
Beta strandi437 – 441Combined sources5
Helixi447 – 466Combined sources20
Helixi470 – 500Combined sources31

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WSXX-ray3.50A/B/C1-504[»]
3HFXX-ray3.15A1-504[»]
ProteinModelPortaliP31553.
SMRiP31553.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31553.

Family & Domainsi

Sequence similaritiesi

Belongs to the BCCT transporter (TC 2.A.15) family. CaiT subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C94. Bacteria.
COG1292. LUCA.
HOGENOMiHOG000053240.
InParanoidiP31553.
KOiK05245.
OMAiCWIVLNA.
PhylomeDBiP31553.

Family and domain databases

HAMAPiMF_01049. CaiT. 1 hit.
InterProiIPR018093. BCCT_CS.
IPR000060. BCCT_transptr.
IPR023449. BCCT_transptr_CaiT.
[Graphical view]
PfamiPF02028. BCCT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00842. bcct. 1 hit.
PROSITEiPS01303. BCCT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31553-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNEKRKTGI EPKVFFPPLI IVGILCWLTV RDLDAANVVI NAVFSYVTNV
60 70 80 90 100
WGWAFEWYMV VMLFGWFWLV FGPYAKKRLG NEPPEFSTAS WIFMMFASCT
110 120 130 140 150
SAAVLFWGSI EIYYYISTPP FGLEPNSTGA KELGLAYSLF HWGPLPWATY
160 170 180 190 200
SFLSVAFAYF FFVRKMEVIR PSSTLVPLVG EKHAKGLFGT IVDNFYLVAL
210 220 230 240 250
IFAMGTSLGL ATPLVTECMQ WLFGIPHTLQ LDAIIITCWI ILNAICVACG
260 270 280 290 300
LQKGVRIASD VRSYLSFLML GWVFIVSGAS FIMNYFTDSV GMLLMYLPRM
310 320 330 340 350
LFYTDPIAKG GFPQGWTVFY WAWWVIYAIQ MSIFLARISR GRTVRELCFG
360 370 380 390 400
MVLGLTASTW ILWTVLGSNT LLLIDKNIIN IPNLIEQYGV ARAIIETWAA
410 420 430 440 450
LPLSTATMWG FFILCFIATV TLVNACSYTL AMSTCREVRD GEEPPLLVRI
460 470 480 490 500
GWSILVGIIG IVLLALGGLK PIQTAIIAGG CPLFFVNIMV TLSFIKDAKQ

NWKD
Length:504
Mass (Da):56,587
Last modified:November 1, 1997 - v2
Checksum:iA852310DBEA22D82
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti353L → M in strain: O44:K74. 1
Natural varianti374I → M in strain: B. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73904 Genomic DNA. Translation: CAA52110.1.
U00096 Genomic DNA. Translation: AAC73151.1.
AP009048 Genomic DNA. Translation: BAB96609.2.
AY625104 Genomic DNA. Translation: AAT42458.1.
PIRiH64724.
RefSeqiNP_414582.1. NC_000913.3.
WP_000787103.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73151; AAC73151; b0040.
BAB96609; BAB96609; BAB96609.
GeneIDi944765.
KEGGiecj:JW0039.
eco:b0040.
PATRICi32115175. VBIEscCol129921_0039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73904 Genomic DNA. Translation: CAA52110.1.
U00096 Genomic DNA. Translation: AAC73151.1.
AP009048 Genomic DNA. Translation: BAB96609.2.
AY625104 Genomic DNA. Translation: AAT42458.1.
PIRiH64724.
RefSeqiNP_414582.1. NC_000913.3.
WP_000787103.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WSXX-ray3.50A/B/C1-504[»]
3HFXX-ray3.15A1-504[»]
ProteinModelPortaliP31553.
SMRiP31553.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260766. 345 interactors.
DIPiDIP-9247N.
STRINGi511145.b0040.

Protein family/group databases

TCDBi2.A.15.2.1. the betaine/carnitine/choline transporter (bcct) family.

Proteomic databases

PaxDbiP31553.
PRIDEiP31553.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73151; AAC73151; b0040.
BAB96609; BAB96609; BAB96609.
GeneIDi944765.
KEGGiecj:JW0039.
eco:b0040.
PATRICi32115175. VBIEscCol129921_0039.

Organism-specific databases

EchoBASEiEB1522.
EcoGeneiEG11561. caiT.

Phylogenomic databases

eggNOGiENOG4105C94. Bacteria.
COG1292. LUCA.
HOGENOMiHOG000053240.
InParanoidiP31553.
KOiK05245.
OMAiCWIVLNA.
PhylomeDBiP31553.

Enzyme and pathway databases

UniPathwayiUPA00117.
BioCyciEcoCyc:CAIT-MONOMER.
ECOL316407:JW0039-MONOMER.
MetaCyc:CAIT-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP31553.
PROiP31553.

Family and domain databases

HAMAPiMF_01049. CaiT. 1 hit.
InterProiIPR018093. BCCT_CS.
IPR000060. BCCT_transptr.
IPR023449. BCCT_transptr_CaiT.
[Graphical view]
PfamiPF02028. BCCT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00842. bcct. 1 hit.
PROSITEiPS01303. BCCT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAIT_ECOLI
AccessioniPrimary (citable) accession number: P31553
Secondary accession number(s): P75624, Q6IU45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.