P31552 (CAIC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable crotonobetaine/carnitine-CoA ligase
EC=6.2.1.-

Gene names

Name:	caiC
Synonyms:	yaaM
Ordered Locus Names:	b0037, JW0036

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Could catalyze the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle. Ref.1 Ref.6 Ref.7
Pathway	Amine and polyamine metabolism; carnitine metabolism. HAMAP MF_01524
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.
Sequence caution	The sequence BAB96606.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA52113.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	carnitine-CoA ligase activity Inferred from direct assay. Source: EcoCyc crotonobetaine-CoA ligase activity Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Probable crotonobetaine/carnitine-CoA ligase HAMAP MF_01524		PRO_0000193065
Natural variations
	Natural variant	98	1	C → R in strain: O44:K74.
	Natural variant	252 – 253	2	RA → PR in strain: O44:K74.
	Natural variant	286	1	Q → R in strain: O44:K74 and B.
	Natural variant	328	1	I → S in strain: O44:K74.
	Natural variant	345	1	V → A in strain: O44:K74 and B.
	Natural variant	359	1	R → C in strain: B.
	Natural variant	374	1	I → V in strain: O44:K74 and B.
	Natural variant	388	1	Q → K in strain: O44:K74 and B.
	Natural variant	396	1	A → V in strain: O44:K74.
	Natural variant	408	1	R → C in strain: O44:K74.
	Natural variant	412	1	D → G in strain: O44:K74 and B.
	Natural variant	442	1	A → T in strain: O44:K74 and B.
	Natural variant	503	1	L → LDL in strain: O44:K74.

Sequences

	Sequence		Length	Mass (Da)
	P31552-1 [UniParc]. Last modified August 21, 2007. Version 2. Checksum: C7A307D16200D8E9	FASTA	517	58,559
	10 20 30 40 50 60 MDIIGGQHLR QMWDDLADVY GHKTALICES SGGVVNRYSY LELNQEINRT ANLFYTLGIR 70 80 90 100 110 120 KGDKVALHLD NCPEFIFCWF GLAKIGAIMV PINARLLCEE SAWILQNSQA CLLVTSAQFY 130 140 150 160 170 180 PMYQQIQQED ATQLRHICLT DVALPADDGV SSFTQLKNQQ PATLCYAPPL STDDTAEILF 190 200 210 220 230 240 TSGTTSRPKG VVITHYNLRF AGYYSAWQCA LRDDDVYLTV MPAFHIDCQC TAAMAAFSAG 250 260 270 280 290 300 ATFVLVEKYS ARAFWGQVQK YRATVTECIP MMIRTLMVQP PSANDQQHRL REVMFYLNLS 310 320 330 340 350 360 EQEKDAFCER FGVRLLTSYG MTETIVGIIG DRPGDKRRWP SIGRVGFCYE AEIRDDHNRP 370 380 390 400 410 420 LPAGEIGEIC IKGIPGKTIF KEYFLNPQAT AKVLEADGWL HTGDTGYRDE EDFFYFVDRR 430 440 450 460 470 480 CNMIKRGGEN VSCVELENII AAHPKIQDIV VVGIKDSIRD EAIKAFVVLN EGETLSEEEF 490 500 510 FRFCEQNMAK FKVPSYLEIR KDLPRNCSGK IIRKNLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli." Eichler K., Bourgis F., Buchet A., Kleber H.-P., Mandrand-Berthelot M.-A. Mol. Microbiol. 13:775-786(1994) [PubMed: 7815937] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROBABLE FUNCTION. Strain: O44:K74.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations." Lenski R.E., Winkworth C.L., Riley M.A. J. Mol. Evol. 56:498-508(2003) [PubMed: 12664169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 285-459. Strain: B.
[6]	"A new family of CoA-transferases." Heider J. FEBS Lett. 509:345-349(2001) [PubMed: 11749953] [Abstract] Cited for: PROBABLE FUNCTION.
[7]	"Crystal structure of CaiB, a type-III CoA transferase in carnitine metabolism." Stenmark P., Gurmu D., Nordlund P. Biochemistry 43:13996-14003(2004) [PubMed: 15518548] [Abstract] Cited for: PROBABLE FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X73904 Genomic DNA. Translation: CAA52113.1. Different initiation. U00096 Genomic DNA. Translation: AAC73148.2. AP009048 Genomic DNA. Translation: BAB96606.1. Different initiation. AY625099 Genomic DNA. Translation: AAT42453.1.
PIR	S40558. E64724.
RefSeq	AP_000701.1. NP_414579.4.
3D structure databases
ProteinModelPortal	P31552.
SMR	P31552. Positions 17-517.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9243N.
STRING	P31552.
Protein family/group databases
TCDB	4.C.1.1.6. proposed fatty acid transporter (FAT) family.
Genome annotation databases
EnsemblBacteria	EBESCT00000001446; EBESCP00000001446; EBESCG00000001208. EBESCT00000017639; EBESCP00000016930; EBESCG00000016695.
GeneID	944886.
GenomeReviews	Gene locus JW0036 in contig AP009048_GR. Gene locus b0037 in contig U00096_GR.
KEGG	ecj:JW0036. eco:b0037.
Organism-specific databases
EchoBASE	EB1519.
EcoGene	EG11558. caiC.
CMR	Search...
Phylogenomic databases
eggNOG	COG0318.
HOGENOM	HBG547964.
OMA	SANDQQH.
ProtClustDB	PRK08008.
Enzyme and pathway databases
BioCyc	EcoCyc:CAIC-MONOMER. MetaCyc:CAIC-MONOMER.
Gene expression databases
Genevestigator	P31552.
Family and domain databases
HAMAP	MF_01524. CaiC. [Tree]
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAIC_ECOLI

Accession

Primary (citable) accession number: P31552
Secondary accession number(s): Q6IU50

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	August 21, 2007
Last modified:	August 10, 2010
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)