Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable crotonobetaine/carnitine-CoA ligase

Gene

caiC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Could catalyze the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle.

Pathwayi: carnitine metabolism

This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.
View all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.

GO - Molecular functioni

  • acid-thiol ligase activity Source: InterPro
  • carnitine-CoA ligase activity Source: EcoCyc
  • crotonobetaine-CoA ligase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Enzyme and pathway databases

BioCyciEcoCyc:CAIC-MONOMER.
ECOL316407:JW0036-MONOMER.
MetaCyc:CAIC-MONOMER.
UniPathwayiUPA00117.

Protein family/group databases

TCDBi4.C.1.1.6. the proposed fatty acid transporter (fat) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable crotonobetaine/carnitine-CoA ligase (EC:6.2.1.-)
Gene namesi
Name:caiC
Synonyms:yaaM
Ordered Locus Names:b0037, JW0036
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11558. caiC.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 517517Probable crotonobetaine/carnitine-CoA ligasePRO_0000193065Add
BLAST

Proteomic databases

EPDiP31552.
PaxDbiP31552.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
allRP0ACN43EBI-1112975,EBI-561736

Protein-protein interaction databases

BioGridi4261017. 531 interactions.
DIPiDIP-9243N.
IntActiP31552. 5 interactions.
STRINGi511145.b0037.

Structurei

3D structure databases

ProteinModelPortaliP31552.
SMRiP31552. Positions 20-517.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CEY. Bacteria.
COG0318. LUCA.
HOGENOMiHOG000230001.
InParanoidiP31552.
KOiK02182.
OMAiTALIYES.
OrthoDBiEOG6MH5BV.
PhylomeDBiP31552.

Family and domain databases

HAMAPiMF_01524. CaiC.
InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR023456. CaiC.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31552-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIIGGQHLR QMWDDLADVY GHKTALICES SGGVVNRYSY LELNQEINRT
60 70 80 90 100
ANLFYTLGIR KGDKVALHLD NCPEFIFCWF GLAKIGAIMV PINARLLCEE
110 120 130 140 150
SAWILQNSQA CLLVTSAQFY PMYQQIQQED ATQLRHICLT DVALPADDGV
160 170 180 190 200
SSFTQLKNQQ PATLCYAPPL STDDTAEILF TSGTTSRPKG VVITHYNLRF
210 220 230 240 250
AGYYSAWQCA LRDDDVYLTV MPAFHIDCQC TAAMAAFSAG ATFVLVEKYS
260 270 280 290 300
ARAFWGQVQK YRATVTECIP MMIRTLMVQP PSANDQQHRL REVMFYLNLS
310 320 330 340 350
EQEKDAFCER FGVRLLTSYG MTETIVGIIG DRPGDKRRWP SIGRVGFCYE
360 370 380 390 400
AEIRDDHNRP LPAGEIGEIC IKGIPGKTIF KEYFLNPQAT AKVLEADGWL
410 420 430 440 450
HTGDTGYRDE EDFFYFVDRR CNMIKRGGEN VSCVELENII AAHPKIQDIV
460 470 480 490 500
VVGIKDSIRD EAIKAFVVLN EGETLSEEEF FRFCEQNMAK FKVPSYLEIR
510
KDLPRNCSGK IIRKNLK
Length:517
Mass (Da):58,559
Last modified:August 21, 2007 - v2
Checksum:iC7A307D16200D8E9
GO

Sequence cautioni

The sequence BAB96606.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA52113.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti98 – 981C → R in strain: O44:K74.
Natural varianti252 – 2532RA → PR in strain: O44:K74.
Natural varianti286 – 2861Q → R in strain: O44:K74 and B.
Natural varianti328 – 3281I → S in strain: O44:K74.
Natural varianti345 – 3451V → A in strain: O44:K74 and B.
Natural varianti359 – 3591R → C in strain: B.
Natural varianti374 – 3741I → V in strain: O44:K74 and B.
Natural varianti388 – 3881Q → K in strain: O44:K74 and B.
Natural varianti396 – 3961A → V in strain: O44:K74.
Natural varianti408 – 4081R → C in strain: O44:K74.
Natural varianti412 – 4121D → G in strain: O44:K74 and B.
Natural varianti442 – 4421A → T in strain: O44:K74 and B.
Natural varianti503 – 5031L → LDL in strain: O44:K74.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73904 Genomic DNA. Translation: CAA52113.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73148.2.
AP009048 Genomic DNA. Translation: BAB96606.1. Different initiation.
AY625099 Genomic DNA. Translation: AAT42453.1.
PIRiE64724. S40558.
RefSeqiNP_414579.4. NC_000913.3.
WP_001350478.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73148; AAC73148; b0037.
BAB96606; BAB96606; BAB96606.
GeneIDi944886.
KEGGiecj:JW0036.
eco:b0037.
PATRICi32115169. VBIEscCol129921_0036.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73904 Genomic DNA. Translation: CAA52113.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73148.2.
AP009048 Genomic DNA. Translation: BAB96606.1. Different initiation.
AY625099 Genomic DNA. Translation: AAT42453.1.
PIRiE64724. S40558.
RefSeqiNP_414579.4. NC_000913.3.
WP_001350478.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP31552.
SMRiP31552. Positions 20-517.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261017. 531 interactions.
DIPiDIP-9243N.
IntActiP31552. 5 interactions.
STRINGi511145.b0037.

Protein family/group databases

TCDBi4.C.1.1.6. the proposed fatty acid transporter (fat) family.

Proteomic databases

EPDiP31552.
PaxDbiP31552.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73148; AAC73148; b0037.
BAB96606; BAB96606; BAB96606.
GeneIDi944886.
KEGGiecj:JW0036.
eco:b0037.
PATRICi32115169. VBIEscCol129921_0036.

Organism-specific databases

EchoBASEiEB1519.
EcoGeneiEG11558. caiC.

Phylogenomic databases

eggNOGiENOG4105CEY. Bacteria.
COG0318. LUCA.
HOGENOMiHOG000230001.
InParanoidiP31552.
KOiK02182.
OMAiTALIYES.
OrthoDBiEOG6MH5BV.
PhylomeDBiP31552.

Enzyme and pathway databases

UniPathwayiUPA00117.
BioCyciEcoCyc:CAIC-MONOMER.
ECOL316407:JW0036-MONOMER.
MetaCyc:CAIC-MONOMER.

Miscellaneous databases

PROiP31552.

Family and domain databases

HAMAPiMF_01524. CaiC.
InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR023456. CaiC.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli."
    Eichler K., Bourgis F., Buchet A., Kleber H.-P., Mandrand-Berthelot M.-A.
    Mol. Microbiol. 13:775-786(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROBABLE FUNCTION.
    Strain: O44:K74.
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
    Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
    Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations."
    Lenski R.E., Winkworth C.L., Riley M.A.
    J. Mol. Evol. 56:498-508(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 285-459.
    Strain: B.
  6. "A new family of CoA-transferases."
    Heider J.
    FEBS Lett. 509:345-349(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROBABLE FUNCTION.
  7. "Crystal structure of CaiB, a type-III CoA transferase in carnitine metabolism."
    Stenmark P., Gurmu D., Nordlund P.
    Biochemistry 43:13996-14003(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROBABLE FUNCTION.

Entry informationi

Entry nameiCAIC_ECOLI
AccessioniPrimary (citable) accession number: P31552
Secondary accession number(s): Q6IU50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: August 21, 2007
Last modified: March 16, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.