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Protein

Carnitinyl-CoA dehydratase

Gene

caiD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA.1 Publication

Catalytic activityi

L-carnitinyl-CoA = (E)-4-(trimethylammonio)but-2-enoyl-CoA + H2O.1 Publication

Kineticsi

  1. KM=12 µM for crotonobetainyl-CoA1 Publication
  2. KM=38 µM for crotonyl-CoA1 Publication
  1. Vmax=186 µmol/min/mg enzyme with crotonobetainyl-CoA as substrate1 Publication
  2. Vmax=0.3 µmol/min/mg enzyme with crotonyl-CoA as substrate1 Publication

Pathwayi: carnitine metabolism

This protein is involved in the pathway carnitine metabolism, which is part of Amine and polyamine metabolism.
View all proteins of this organism that are known to be involved in the pathway carnitine metabolism and in Amine and polyamine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei111Important for catalytic activityBy similarity1
Sitei131Important for catalytic activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase

Enzyme and pathway databases

BioCyciEcoCyc:CARNRACE-MONOMER
MetaCyc:CARNRACE-MONOMER
BRENDAi4.2.1.149 2026
UniPathwayiUPA00117

Names & Taxonomyi

Protein namesi
Recommended name:
Carnitinyl-CoA dehydratase (EC:4.2.1.149)
Alternative name(s):
Crotonobetainyl-CoA hydratase
Gene namesi
Name:caiD
Synonyms:yaaL
Ordered Locus Names:b0036, JW0035
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11557 caiD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001093482 – 261Carnitinyl-CoA dehydrataseAdd BLAST260

Proteomic databases

PaxDbiP31551
PRIDEiP31551

Interactioni

Subunit structurei

Homotrimer.1 Publication

Protein-protein interaction databases

BioGridi4261692, 569 interactors
IntActiP31551, 9 interactors
STRINGi316385.ECDH10B_0037

Structurei

3D structure databases

ProteinModelPortaliP31551
SMRiP31551
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4106YT9 Bacteria
COG1024 LUCA
HOGENOMiHOG000027939
InParanoidiP31551
KOiK08299
OMAiKGRAMEM
PhylomeDBiP31551

Family and domain databases

Gene3Di1.10.12.10, 1 hit
HAMAPiMF_01051 CaiD, 1 hit
InterProiView protein in InterPro
IPR022852 Carnitinyl_CoA_dehydratase
IPR029045 ClpP/crotonase-like_dom_sf
IPR018376 Enoyl-CoA_hyd/isom_CS
IPR001753 Enoyl-CoA_hydra/iso
IPR014748 enoyl-CoA_hydra_C
PfamiView protein in Pfam
PF00378 ECH_1, 1 hit
SUPFAMiSSF52096 SSF52096, 1 hit
PROSITEiView protein in PROSITE
PS00166 ENOYL_COA_HYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSESLHLTRN GSILEITLDR PKANAIDAKT SFEMGEVFLN FRDDPQLRVA
60 70 80 90 100
IITGAGEKFF SAGWDLKAAA EGEAPDADFG PGGFAGLTEI FNLDKPVIAA
110 120 130 140 150
VNGYAFGGGF ELALAADFIV CADNASFALP EAKLGIVPDS GGVLRLPKIL
160 170 180 190 200
PPAIVNEMVM TGRRMGAEEA LRWGIVNRVV SQAELMDNAR ELAQQLVNSA
210 220 230 240 250
PLAIAALKEI YRTTSEMPVE EAYRYIRSGV LKHYPSVLHS EDAIEGPLAF
260
AEKRDPVWKG R
Length:261
Mass (Da):28,190
Last modified:January 23, 2007 - v4
Checksum:i2C4DD6C3D16995CC
GO

Sequence cautioni

The sequence BAB96605 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA52114 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti247P → L in strain: O44:K74. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73904 Genomic DNA Translation: CAA52114.1 Different initiation.
U00096 Genomic DNA Translation: AAC73147.2
AP009048 Genomic DNA Translation: BAB96605.2 Different initiation.
PIRiD64724
RefSeqiNP_414578.2, NC_000913.3
WP_001295419.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73147; AAC73147; b0036
BAB96605; BAB96605; BAB96605
GeneIDi948995
KEGGiecj:JW0035
eco:b0036
PATRICifig|1411691.4.peg.2247

Similar proteinsi

Entry informationi

Entry nameiCAID_ECOLI
AccessioniPrimary (citable) accession number: P31551
Secondary accession number(s): P75623
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 147 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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