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P31550 (THIB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thiamine-binding periplasmic protein
Gene names
Name:thiB
Synonyms:tbpA, yabL
Ordered Locus Names:b0068, JW0067
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the ABC transporter complex thiBPQ involved in thiamine import. Ref.5

Subunit structure

The complex is composed of two ATP-binding proteins (thiQ), two transmembrane proteins (thiP) and a solute-binding protein (thiB) Probable.

Subcellular location

Periplasm.

Sequence similarities

Belongs to the bacterial solute-binding protein 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818
Chain19 – 327309Thiamine-binding periplasmic protein
PRO_0000031705

Experimental info

Sequence conflict1 – 1212MLKKC…LLLLC → MSAPAVAV AA sequence Ref.1

Secondary structure

1 ........................................................ 327
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P31550 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 348A5625FFC94597

FASTA32736,163
        10         20         30         40         50         60 
MLKKCLPLLL LCTAPVFAKP VLTVYTYDSF AADWGPGPVV KKAFEADCNC ELKLVALEDG 

        70         80         90        100        110        120 
VSLLNRLRME GKNSKADVVL GLDNNLLDAA SKTGLFAKSG VAADAVNVPG GWNNDTFVPF 

       130        140        150        160        170        180 
DYGYFAFVYD KNKLKNPPQS LKELVESDQN WRVIYQDPRT STPGLGLLLW MQKVYGDDAP 

       190        200        210        220        230        240 
QAWQKLAKKT VTVTKGWSEA YGLFLKGESD LVLSYTTSPA YHILEEKKDN YAAANFSEGH 

       250        260        270        280        290        300 
YLQVEVAART AASKQPELAQ KFLQFMVSPA FQNAIPTGNW MYPVANVTLP AGFEKLTKPA 

       310        320 
TTLEFTPAEV AAQRQAWISE WQRAVSR

« Hide

References

« Hide 'large scale' references
[1]"E. coli periplasmic thiamin binding protein: cloning, overexpression, purification, and characterization."
Hollenbach A.D., Dickson K.A., Washabaugh M.W.
Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
Strain: K12 / C600 / ATCC 23724 / DSM 3925 / LMG 3041 / NCIB 10222.
[2]"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 1-12.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"thiBPQ encodes an ABC transporter required for transport of thiamine and thiamine pyrophosphate in Salmonella typhimurium."
Webb E., Claas K., Downs D.
J. Biol. Chem. 273:8946-8950(1998) [PubMed: 9535878] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U09984 Genomic DNA. Translation: AAA18833.1.
U00096 Genomic DNA. Translation: AAC73179.1.
AP009048 Genomic DNA. Translation: BAB96637.2.
PIRD64728.
RefSeqNP_414610.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2QRYX-ray2.25A/B/C/D19-327[»]
ProteinModelPortalP31550.
SMRP31550. Positions 19-327.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10967N.
IntActP31550. 3 interactions.

Protein family/group databases

TCDB3.A.1.19.1. ATP-binding cassette (ABC) superfamily.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000001530; EBESCP00000001530; EBESCG00000001273.
EBESCT00000015904; EBESCP00000015195; EBESCG00000014964.
GeneID946306.
GenomeReviewsGene locus JW0067 in contig AP009048_GR.
Gene locus b0068 in contig U00096_GR.
KEGGecj:JW0067.
eco:b0068.
PATRIC32115237. VBIEscCol129921_0070.

Organism-specific databases

EchoBASEEB1534.
EcoGeneEG11574. tbpA.

Phylogenomic databases

eggNOGCOG4143.
GeneTreeEBGT00050000011418.
HOGENOMHBG476839.
OMAIPTTNWM.
PhylomeDBP31550.
ProtClustDBPRK11205.

Enzyme and pathway databases

BioCycEcoCyc:SFUA-MONOMER.

Gene expression databases

GenevestigatorP31550.

Family and domain databases

InterProIPR006059. Solute-bd_1_bac.
IPR006061. Solute-bd_1_bac_CS.
IPR005948. Thi_ABC_peri-bd.
IPR005967. ThiB_ABC_peri-bd.
[Graphical view]
KOK02064.
PfamPF01547. SBP_bac_1. 1 hit.
[Graphical view]
TIGRFAMsTIGR01254. SfuA. 1 hit.
TIGR01276. ThiB. 1 hit.
PROSITEPS01037. SBP_BACTERIAL_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTHIB_ECOLI
AccessionPrimary (citable) accession number: P31550
Secondary accession number(s): P75637
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: January 25, 2012
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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