P31549 (THIP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiamine transport system permease protein thiP

Gene names

Name:	thiP
Synonyms:	yabK
Ordered Locus Names:	b0067, JW0066

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane. Ref.4
Subunit structure	The complex is composed of two ATP-binding proteins (thiQ), two transmembrane proteins (thiP) and a solute-binding protein (thiB) Probable.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.5.
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily. Contains 2 ABC transmembrane type-1 domains.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	Thiamine transport system permease protein thiP		PRO_0000060230
Regions
	Transmembrane	12 – 32	21	Helical; Potential
	Transmembrane	58 – 78	21	Helical; Potential
	Transmembrane	95 – 115	21	Helical; Potential
	Transmembrane	134 – 154	21	Helical; Potential
	Transmembrane	199 – 219	21	Helical; Potential
	Transmembrane	240 – 260	21	Helical; Potential
	Transmembrane	293 – 313	21	Helical; Potential
	Transmembrane	334 – 354	21	Helical; Potential
	Transmembrane	374 – 394	21	Helical; Potential
	Transmembrane	404 – 424	21	Helical; Potential
	Transmembrane	463 – 483	21	Helical; Potential
	Transmembrane	506 – 526	21	Helical; Potential
	Domain	56 – 261	206	ABC transmembrane type-1 1
	Domain	331 – 525	195	ABC transmembrane type-1 2

Sequences

	Sequence		Length	Mass (Da)
	P31549 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: F6F559EDA92B8C2A	FASTA	536	59,533
	10 20 30 40 50 60 MATRRQPLIP GWLIPGVSAT TLVVAVALAA FLALWWNAPQ DDWVAVWQDS YLWHVVRFSF 70 80 90 100 110 120 WQAFLSALLS VIPAIFLARA LYRRRFPGRL ALLRLCAMTL ILPVLVAVFG ILSVYGRQGW 130 140 150 160 170 180 LATLCQSLGL EWTFSPYGLQ GILLAHVFFN LPMASRLLLQ ALENIPGEQR QLAAQLGMRS 190 200 210 220 230 240 WHFFRFVEWP WLRRQIPPVA ALIFMLCFAS FATVLSLGGG PQATTIELAI YQALSYDYDP 250 260 270 280 290 300 ARAAMLALLQ MVCCLGLVLL SQRLSKAIAP GTTLLQGWRD PDDRLHSRIC DTVLIVLALL 310 320 330 340 350 360 LLLPPLLAVI VDGVNRQLPE VLAQPVLWQA LWTSLRIALA AGVLCVVLTM MLLWSSRELR 370 380 390 400 410 420 ARQKMLAGQV LEMSGMLILA MPGIVLATGF FLLLNNTIGL PQSADGIVIF TNALMAIPYA 430 440 450 460 470 480 LKVLENPMRD ITARYSMLCQ SLGIEGWSRL KVVELRALKR PLAQALAFAC VLSIGDFGVV 490 500 510 520 530 ALFGNDDFRT LPFYLYQQIG SYRSQDGAVT ALILLLLCFL LFTVIEKLPG RNVKTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 511. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"thiBPQ encodes an ABC transporter required for transport of thiamine and thiamine pyrophosphate in Salmonella typhimurium." Webb E., Claas K., Downs D. J. Biol. Chem. 273:8946-8950(1998) [PubMed: 9535878] [Abstract] Cited for: FUNCTION.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73178.1. AP009048 Genomic DNA. Translation: BAB96636.2.
PIR	C64728.
RefSeq	NP_414609.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P31549.
SMR	P31549. Positions 14-264.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10989N.
Protein family/group databases
TCDB	3.A.1.19.1. ATP-binding cassette (ABC) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002612; EBESCP00000002612; EBESCG00000002132. EBESCT00000016895; EBESCP00000016186; EBESCG00000015954.
GeneID	944784.
GenomeReviews	Gene locus JW0066 in contig AP009048_GR. Gene locus b0067 in contig U00096_GR.
KEGG	ecj:JW0066. eco:b0067.
PATRIC	32115235. VBIEscCol129921_0069.
Organism-specific databases
EchoBASE	EB1533.
EcoGene	EG11573. thiP.
Phylogenomic databases
eggNOG	COG1178.
GeneTree	EBGT00050000008926.
HOGENOM	HBG296626.
OMA	HDSYLWH.
PhylomeDB	P31549.
ProtClustDB	PRK09433.
Enzyme and pathway databases
BioCyc	EcoCyc:SFUB-MONOMER.
Gene expression databases
Genevestigator	P31549.
Family and domain databases
InterPro	IPR000515. BPD_transp. IPR005947. ThiP_ABC_transpt. [Graphical view]
KO	K02063.
Pfam	PF00528. BPD_transp_1. 2 hits. [Graphical view]
TIGRFAMs	TIGR01253. ThiP. 1 hit.
PROSITE	PS50928. ABC_TM1. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIP_ECOLI

Accession

Primary (citable) accession number: P31549
Secondary accession number(s): P75636

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program