P31548 (THIQ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiamine import ATP-binding protein ThiQ
EC=3.6.3.-

Gene names

Name:	thiQ
Synonyms:	yabJ
Ordered Locus Names:	b0066, JW0065

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the ABC transporter complex ThiBPQ involved in thiamine import. Responsible for energy coupling to the transport system Probable. Ref.5
Subunit structure	The complex is composed of two ATP-binding proteins (ThiQ), two transmembrane proteins (ThiP) and a solute-binding protein (ThiB) Potential.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity HAMAP MF_01723.
Sequence similarities	Belongs to the ABC transporter superfamily. Thiamine importer (TC 3.A.1.19.1) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity, coupled to transmembrane movement of substances Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Thiamine import ATP-binding protein ThiQ HAMAP MF_01723		PRO_0000093022
Regions
	Domain	2 – 230	229	ABC transporter
	Nucleotide binding	32 – 39	8	ATP Potential
Experimental info
	Sequence conflict	66	1	M → T in AAT42474. Ref.4
	Sequence conflict	105	1	V → A in AAT42474. Ref.4
	Sequence conflict	108	1	G → E in AAT42474. Ref.4
	Sequence conflict	213	1	M → K in AAT42474. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P31548 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 22234D38F57B7C68	FASTA	232	24,999
	10 20 30 40 50 60 MLKLTDITWL YHHLPMRFSL TVERGEQVAI LGPSGAGKST LLNLIAGFLT PASGSLTIDG 70 80 90 100 110 120 VDHTTMPPSR RPVSMLFQEN NLFSHLTVAQ NIGLGLNPGL KLNAVQQGKM HAIARQMGID 130 140 150 160 170 180 NLMARLPGEL SGGQRQRVAL ARCLVREQPI LLLDEPFSAL DPALRQEMLT LVSTSCQQQK 190 200 210 220 230 MTLLMVSHSV EDAARIATRS VVVADGRIAW QGMTNELLSG KASASALLGI TG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 156. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations." Lenski R.E., Winkworth C.L., Riley M.A. J. Mol. Evol. 56:498-508(2003) [PubMed: 12664169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 51-214. Strain: B / Bc251.
[5]	"Thiamine transport in Escherichia coli: the mechanism of inhibition by the sulfhydryl-specific modifier N-ethylmaleimide." Hollenbach A.D., Dickson K.A., Washabaugh M.W. Biochim. Biophys. Acta 1564:421-428(2002) [PubMed: 12175925] [Abstract] Cited for: FUNCTION IN THIAMINE TRANSPORT. Strain: K12 / KG33.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73177.1. AP009048 Genomic DNA. Translation: BAB96635.2. AY625120 Genomic DNA. Translation: AAT42474.1.
PIR	B64728.
RefSeq	NP_414608.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P31548.
SMR	P31548. Positions 2-229.
ModBase	Search...
Protein-protein interaction databases
IntAct	P31548. 6 interactions.
Protein family/group databases
TCDB	3.A.1.19.1. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PRIDE	P31548.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001901; EBESCP00000001901; EBESCG00000001561. EBESCT00000017592; EBESCP00000016883; EBESCG00000016648.
GeneID	944785.
GenomeReviews	Gene locus JW0065 in contig AP009048_GR. Gene locus b0066 in contig U00096_GR.
KEGG	ecj:JW0065. eco:b0066.
PATRIC	32115233. VBIEscCol129921_0068.
Organism-specific databases
EchoBASE	EB1532.
EcoGene	EG11572. thiQ.
Phylogenomic databases
eggNOG	COG3840.
GeneTree	EBGT00050000008242.
HOGENOM	HBG758042.
OMA	SASAIWG.
PhylomeDB	P31548.
ProtClustDB	PRK10771.
Enzyme and pathway databases
BioCyc	EcoCyc:SFUC-MONOMER.
Gene expression databases
Genevestigator	P31548.
Family and domain databases
HAMAP	MF_01723. ABC_ATPase_ThiQ. [Tree]
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR005968. ABC_transptr_thiamine_ATP-bd. IPR003593. ATPase_AAA+_core. [Graphical view]
KO	K02062.
PANTHER	PTHR24220:SF58. PTHR24220:SF58. 1 hit.
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01277. ThiQ. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51288. THIQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THIQ_ECOLI

Accession

Primary (citable) accession number: P31548
Secondary accession number(s): P75635, Q6IU29

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program