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Protein

Thiamine import ATP-binding protein ThiQ

Gene

thiQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex ThiBPQ involved in thiamine import. Responsible for energy coupling to the transport system (Probable).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi32 – 39ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:SFUC-MONOMER.
ECOL316407:JW0065-MONOMER.

Protein family/group databases

TCDBi3.A.1.19.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine import ATP-binding protein ThiQUniRule annotation (EC:3.6.3.-UniRule annotation)
Gene namesi
Name:thiQUniRule annotation
Synonyms:yabJ
Ordered Locus Names:b0066, JW0065
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11572. thiQ.

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000930221 – 232Thiamine import ATP-binding protein ThiQAdd BLAST232

Proteomic databases

PaxDbiP31548.
PRIDEiP31548.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (ThiQ), two transmembrane proteins (ThiP) and a solute-binding protein (ThiB).UniRule annotation

Protein-protein interaction databases

BioGridi4259729. 5 interactors.
IntActiP31548. 6 interactors.
STRINGi511145.b0066.

Structurei

3D structure databases

ProteinModelPortaliP31548.
SMRiP31548.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 230ABC transporterUniRule annotationAdd BLAST229

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Thiamine importer (TC 3.A.1.19.1) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107T0D. Bacteria.
COG3840. LUCA.
InParanoidiP31548.
KOiK02062.
OMAiACCLVRE.
PhylomeDBiP31548.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR005968. Thiamine_ABC_ThiQ.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01277. thiQ. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51288. THIQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKLTDITWL YHHLPMRFSL TVERGEQVAI LGPSGAGKST LLNLIAGFLT
60 70 80 90 100
PASGSLTIDG VDHTTMPPSR RPVSMLFQEN NLFSHLTVAQ NIGLGLNPGL
110 120 130 140 150
KLNAVQQGKM HAIARQMGID NLMARLPGEL SGGQRQRVAL ARCLVREQPI
160 170 180 190 200
LLLDEPFSAL DPALRQEMLT LVSTSCQQQK MTLLMVSHSV EDAARIATRS
210 220 230
VVVADGRIAW QGMTNELLSG KASASALLGI TG
Length:232
Mass (Da):24,999
Last modified:November 1, 1997 - v2
Checksum:i22234D38F57B7C68
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66M → T in AAT42474 (PubMed:12664169).Curated1
Sequence conflicti105V → A in AAT42474 (PubMed:12664169).Curated1
Sequence conflicti108G → E in AAT42474 (PubMed:12664169).Curated1
Sequence conflicti213M → K in AAT42474 (PubMed:12664169).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73177.1.
AP009048 Genomic DNA. Translation: BAB96635.2.
AY625120 Genomic DNA. Translation: AAT42474.1.
PIRiB64728.
RefSeqiNP_414608.1. NC_000913.3.
WP_000916281.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73177; AAC73177; b0066.
BAB96635; BAB96635; BAB96635.
GeneIDi944785.
KEGGiecj:JW0065.
eco:b0066.
PATRICi32115233. VBIEscCol129921_0068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73177.1.
AP009048 Genomic DNA. Translation: BAB96635.2.
AY625120 Genomic DNA. Translation: AAT42474.1.
PIRiB64728.
RefSeqiNP_414608.1. NC_000913.3.
WP_000916281.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP31548.
SMRiP31548.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259729. 5 interactors.
IntActiP31548. 6 interactors.
STRINGi511145.b0066.

Protein family/group databases

TCDBi3.A.1.19.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP31548.
PRIDEiP31548.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73177; AAC73177; b0066.
BAB96635; BAB96635; BAB96635.
GeneIDi944785.
KEGGiecj:JW0065.
eco:b0066.
PATRICi32115233. VBIEscCol129921_0068.

Organism-specific databases

EchoBASEiEB1532.
EcoGeneiEG11572. thiQ.

Phylogenomic databases

eggNOGiENOG4107T0D. Bacteria.
COG3840. LUCA.
InParanoidiP31548.
KOiK02062.
OMAiACCLVRE.
PhylomeDBiP31548.

Enzyme and pathway databases

BioCyciEcoCyc:SFUC-MONOMER.
ECOL316407:JW0065-MONOMER.

Miscellaneous databases

PROiP31548.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR005968. Thiamine_ABC_ThiQ.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01277. thiQ. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51288. THIQ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHIQ_ECOLI
AccessioniPrimary (citable) accession number: P31548
Secondary accession number(s): P75635, Q6IU29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.