D-methionine transport system permease protein MetI

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P31547 (METI_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
D-methionine transport system permease protein MetI

Gene names

Name:	metI
Synonyms:	yaeE
Ordered Locus Names:	b0198, JW0194

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the binding-protein-dependent transport system for D-methionine and the toxic methionine analog alpha-methyl-methionine. Probably responsible for the translocation of the substrate across the membrane. Ref.6
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily. Contains 1 ABC transmembrane type-1 domain.
Sequence caution	The sequence BAA03658.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 217

217

D-methionine transport system permease protein MetI

PRO_0000060098

Regions

Topological domain

1 – 19

Periplasmic Potential

Transmembrane

20 – 40

Helical; Potential

Topological domain

41 – 57

Cytoplasmic Potential

Transmembrane

58 – 78

Helical; Potential

Topological domain

79 – 80

Periplasmic Potential

Transmembrane

81 – 101

Helical; Potential

Topological domain

102 – 151

Cytoplasmic Potential

Transmembrane

152 – 172

Helical; Potential

Topological domain

173 – 185

Periplasmic Potential

Transmembrane

186 – 206

Helical; Potential

Topological domain

207 – 217

Cytoplasmic Potential

Domain

13 – 204

192

ABC transmembrane type-1

Secondary structure

217

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P31547 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: EC1A22D772468EA8
Show »

FASTA

217

23,256

        10         20         30         40         50         60 
MSEPMMWLLV RGVWETLAMT FVSGFFGFVI GLPVGVLLYV TRPGQIIANA KLYRTVSAIV 

        70         80         90        100        110        120 
NIFRSIPFII LLVWMIPFTR VIVGTSIGLQ AAIVPLTVGA APFIARMVEN ALLEIPTGLI 

       130        140        150        160        170        180 
EASRAMGATP MQIVRKVLLP EALPGLVNAA TITLITLVGY SAMGGAVGAG GLGQIGYQYG 

       190        200        210 
YIGYNATVMN TVLVLLVILV YLIQFAGDRI VRAVTRK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Miyamoto K. Submitted (APR-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"The metD D-methionine transporter locus of Escherichia coli is an ABC transporter gene cluster." Gal J., Szvetnik A., Schnell R., Kalman M. J. Bacteriol. 184:4930-4932(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: K12 / MG1655 / ATCC 47076.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D15061 Genomic DNA. Translation: BAA03658.1. Different initiation.
U70214 Genomic DNA. Translation: AAB08626.1.
U00096 Genomic DNA. Translation: AAC73309.1.
AP009048 Genomic DNA. Translation: BAA77875.1.

PIR

F64744.

RefSeq

NP_414740.1. NC_000913.2.
YP_488501.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DHW	X-ray	3.70	A/B/E/F	1-217	[»]
3TUI	X-ray	2.90	A/B/E/F	1-217	[»]
3TUJ	X-ray	4.00	A/B	1-217	[»]
3TUZ	X-ray	3.40	A/B/E/F	1-217	[»]

ProteinModelPortal

P31547.

SMR

P31547. Positions 1-216.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-11198N.

STRING

511145.b0198.

Protein family/group databases

TCDB

3.A.1.24.1. ATP-binding cassette (ABC) superfamily.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73309; AAC73309; b0198.
BAA77875; BAA77875; BAA77875.

GeneID

12930760.
944894.

KEGG

ecj:Y75_p0195.
eco:b0198.

PATRIC

32115509. VBIEscCol129921_0206.

Organism-specific databases

EchoBASE

EB1688.

EcoGene

EG11737. metI.

Phylogenomic databases

eggNOG

COG2011.

HOGENOM

HOG000222815.

K02072.

OMA

MIPFTRA.

ProtClustDB

PRK10782.

Enzyme and pathway databases

BioCyc

EcoCyc:METI-MONOMER.
ECOL316407:JW0194-MONOMER.

Gene expression databases

Genevestigator

P31547.

Family and domain databases

InterPro

IPR000515. BPD_transp.
[Graphical view]

Pfam

PF00528. BPD_transp_1. 1 hit.
[Graphical view]

PROSITE

PS50928. ABC_TM1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P31547.

Entry information

Entry name

METI_ECOLI

Accession

Primary (citable) accession number: P31547

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents