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Protein

D-methionine transport system permease protein MetI

Gene

metI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the binding-protein-dependent transport system for D-methionine and the toxic methionine analog alpha-methyl-methionine. Probably responsible for the translocation of the substrate across the membrane.1 Publication

GO - Molecular functioni

  • L-methionine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • D-methionine transport Source: CACAO

Keywordsi

Biological processAmino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:METI-MONOMER.
MetaCyc:METI-MONOMER.

Protein family/group databases

TCDBi3.A.1.24.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
D-methionine transport system permease protein MetI
Gene namesi
Name:metI
Synonyms:yaeE
Ordered Locus Names:b0198, JW0194
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11737. metI.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 19PeriplasmicSequence analysisAdd BLAST19
Transmembranei20 – 40HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini41 – 57CytoplasmicSequence analysisAdd BLAST17
Transmembranei58 – 78HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini79 – 80PeriplasmicSequence analysis2
Transmembranei81 – 101HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini102 – 151CytoplasmicSequence analysisAdd BLAST50
Transmembranei152 – 172HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini173 – 185PeriplasmicSequence analysisAdd BLAST13
Transmembranei186 – 206HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini207 – 217CytoplasmicSequence analysisAdd BLAST11

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000600981 – 217D-methionine transport system permease protein MetIAdd BLAST217

Proteomic databases

PaxDbiP31547.
PRIDEiP31547.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
metNP307505EBI-8769561,EBI-541886

Protein-protein interaction databases

BioGridi4259754. 4 interactors.
DIPiDIP-11198N.
IntActiP31547. 2 interactors.
STRINGi316385.ECDH10B_0179.

Structurei

Secondary structure

1217
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 40Combined sources38
Beta strandi45 – 47Combined sources3
Helixi50 – 64Combined sources15
Helixi68 – 74Combined sources7
Helixi76 – 83Combined sources8
Beta strandi86 – 88Combined sources3
Helixi89 – 112Combined sources24
Helixi118 – 125Combined sources8
Helixi130 – 136Combined sources7
Helixi138 – 163Combined sources26
Turni164 – 168Combined sources5
Helixi171 – 180Combined sources10
Helixi186 – 211Combined sources26

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DHWX-ray3.70A/B/E/F1-217[»]
3TUIX-ray2.90A/B/E/F1-217[»]
3TUJX-ray4.00A/B1-217[»]
3TUZX-ray3.40A/B/E/F1-217[»]
ProteinModelPortaliP31547.
SMRiP31547.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31547.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 204ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST192

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108UMI. Bacteria.
COG2011. LUCA.
HOGENOMiHOG000222815.
InParanoidiP31547.
KOiK02072.
PhylomeDBiP31547.

Family and domain databases

CDDicd06261. TM_PBP2. 1 hit.
Gene3Di1.10.3720.10. 1 hit.
InterProiView protein in InterPro
IPR000515. MetI-like.
IPR035906. MetI-like_sf.
PfamiView protein in Pfam
PF00528. BPD_transp_1. 1 hit.
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiView protein in PROSITE
PS50928. ABC_TM1. 1 hit.

Sequencei

Sequence statusi: Complete.

P31547-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPMMWLLV RGVWETLAMT FVSGFFGFVI GLPVGVLLYV TRPGQIIANA
60 70 80 90 100
KLYRTVSAIV NIFRSIPFII LLVWMIPFTR VIVGTSIGLQ AAIVPLTVGA
110 120 130 140 150
APFIARMVEN ALLEIPTGLI EASRAMGATP MQIVRKVLLP EALPGLVNAA
160 170 180 190 200
TITLITLVGY SAMGGAVGAG GLGQIGYQYG YIGYNATVMN TVLVLLVILV
210
YLIQFAGDRI VRAVTRK
Length:217
Mass (Da):23,256
Last modified:July 1, 1993 - v1
Checksum:iEC1A22D772468EA8
GO

Sequence cautioni

The sequence BAA03658 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D15061 Genomic DNA. Translation: BAA03658.1. Different initiation.
U70214 Genomic DNA. Translation: AAB08626.1.
U00096 Genomic DNA. Translation: AAC73309.1.
AP009048 Genomic DNA. Translation: BAA77875.1.
PIRiF64744.
RefSeqiNP_414740.1. NC_000913.3.
WP_001294600.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73309; AAC73309; b0198.
BAA77875; BAA77875; BAA77875.
GeneIDi944894.
KEGGiecj:JW0194.
eco:b0198.
PATRICifig|1411691.4.peg.2080.

Similar proteinsi

Entry informationi

Entry nameiMETI_ECOLI
AccessioniPrimary (citable) accession number: P31547
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 25, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families