ID   P47K_PSECL              Reviewed;         419 AA.
AC   P31521;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-MAY-2023, entry version 63.
DE   RecName: Full=Zinc chaperone P47K;
DE            EC=3.6.5.- {ECO:0000250|UniProtKB:P24203};
DE   AltName: Full=47 kDa protein {ECO:0000303|PubMed:2013568};
DE            Short=P47K {ECO:0000303|PubMed:2013568};
OS   Pseudomonas chlororaphis (Pseudomonas aureofaciens).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B23;
RX   PubMed=2013568; DOI=10.1128/jb.173.8.2465-2472.1991;
RA   Nishiyama M., Horinouchi S., Kobayashi M., Nagasawa T., Yamada H.,
RA   Beppu T.;
RT   "Cloning and characterization of genes responsible for metabolism of
RT   nitrile compounds from Pseudomonas chlororaphis B23.";
RL   J. Bacteriol. 173:2465-2472(1991).
CC   -!- FUNCTION: Zinc chaperone that directly transfers zinc cofactor to
CC       target proteins, thereby activating them. Zinc is transferred from the
CC       CXCC motif in the GTPase domain to the zinc binding site in target
CC       proteins in a process requiring GTP hydrolysis.
CC       {ECO:0000250|UniProtKB:Q8VEH6}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58189;
CC         Evidence={ECO:0000250|UniProtKB:P24203};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19670;
CC         Evidence={ECO:0000250|UniProtKB:P24203};
CC   -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. ZNG1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; D90216; BAA14247.1; -; Genomic_DNA.
DR   PIR; D42725; D42725.
DR   AlphaFoldDB; P31521; -.
DR   SMR; P31521; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   CDD; cd03112; CobW-like; 1.
DR   Gene3D; 3.30.1220.10; CobW-like, C-terminal domain; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR036627; CobW-likC_sf.
DR   InterPro; IPR011629; CobW-like_C.
DR   InterPro; IPR003495; CobW/HypB/UreG_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR43603; COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527; 1.
DR   PANTHER; PTHR43603:SF1; COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527; 1.
DR   Pfam; PF02492; cobW; 1.
DR   Pfam; PF07683; CobW_C; 1.
DR   SMART; SM00833; CobW_C; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Hydrolase; Nucleotide-binding.
FT   CHAIN           1..419
FT                   /note="Zinc chaperone P47K"
FT                   /id="PRO_0000058140"
FT   DOMAIN          262..379
FT                   /note="CobW C-terminal"
FT   MOTIF           76..79
FT                   /note="CXCC motif"
FT                   /evidence="ECO:0000255"
FT   BINDING         17..24
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
FT   BINDING         79..83
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
FT   BINDING         187..190
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   419 AA;  46666 MW;  FF5113800E27FF0C CRC64;
     MIEGAQAGRL PVTVLSGFLG AGKTTLLNAI LRNRQGLRVA VIVNDMSEVN LDAESVQRDV
     SLHRGRDELI EMSNGCICCT LRADLLEQIS DLARQQRFDY LLIESTGISE PMPVAETFAF
     LDTEGFSLSE LARLDTLVTV VDGSQFQALL ESTDTVARAD TEAHTSTRHL ADLLIEQVEY
     ANVILVNKRD LIDEPGYQAV HAILAGLNPS ARIMPMAHGN VALSSLLDTH LFDLPSLAAS
     PGWMRKMEAT DTPASESDTY GVTSWVYRER APFHPQRLLE FLQKPWHNGR LLRSKGYFWL
     ASRHLEIGLL AQSGKQFQWD YVGRWWNFIE PSQWPRDEYR LQGIMAKWDS VVGDCRQELV
     FIGQGLDTRV LQRELDHCLL SAQEIAAGPL AWQALPAATA FDTEALSARP TPPMAVQPT
//