P31521 (P47K_PSECL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: 47 kDa protein Alternative name(s): P47K
Organism	Pseudomonas chlororaphis (Pseudomonas aureofaciens)
Taxonomic identifier	333 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas chlororaphis group › Pseudomonas chlororaphis

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The presence of P47K is critical for the expression of the nitrile hydratase genes. May stabilize or activate the nitrile hydratase proteins.
Sequence similarities	Belongs to the CobW family. Contains 1 cobW C-terminal domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	47 kDa protein		PRO_0000058140
Regions
	Domain	262 – 379	118	CobW C-terminal
	Nucleotide binding	17 – 24	8	ATP Potential
	Nucleotide binding	79 – 83	5	ATP Potential
	Nucleotide binding	187 – 190	4	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P31521 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: FF5113800E27FF0C	FASTA	419	46,666
	10 20 30 40 50 60 MIEGAQAGRL PVTVLSGFLG AGKTTLLNAI LRNRQGLRVA VIVNDMSEVN LDAESVQRDV 70 80 90 100 110 120 SLHRGRDELI EMSNGCICCT LRADLLEQIS DLARQQRFDY LLIESTGISE PMPVAETFAF 130 140 150 160 170 180 LDTEGFSLSE LARLDTLVTV VDGSQFQALL ESTDTVARAD TEAHTSTRHL ADLLIEQVEY 190 200 210 220 230 240 ANVILVNKRD LIDEPGYQAV HAILAGLNPS ARIMPMAHGN VALSSLLDTH LFDLPSLAAS 250 260 270 280 290 300 PGWMRKMEAT DTPASESDTY GVTSWVYRER APFHPQRLLE FLQKPWHNGR LLRSKGYFWL 310 320 330 340 350 360 ASRHLEIGLL AQSGKQFQWD YVGRWWNFIE PSQWPRDEYR LQGIMAKWDS VVGDCRQELV 370 380 390 400 410 FIGQGLDTRV LQRELDHCLL SAQEIAAGPL AWQALPAATA FDTEALSARP TPPMAVQPT

References

[1]

"Cloning and characterization of genes responsible for metabolism of nitrile compounds from Pseudomonas chlororaphis B23." Nishiyama M., Horinouchi S., Kobayashi M., Nagasawa T., Yamada H., Beppu T. J. Bacteriol. 173:2465-2472(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B23.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D90216 Genomic DNA. Translation: BAA14247.1.
PIR	D42725.
3D structure databases
ProteinModelPortal	P31521.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.1220.10. 1 hit.
InterPro	IPR011629. Cbl_biosynth_CobW-like_C. IPR003495. CobW/HypB/UreG_dom. [Graphical view]
Pfam	PF02492. cobW. 1 hit. PF07683. CobW_C. 1 hit. [Graphical view]
SMART	SM00833. CobW_C. 1 hit. [Graphical view]
SUPFAM	SSF90002. Cbl_biosynth_CobW-like_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

P47K_PSECL

Accession

Primary (citable) accession number: P31521

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program