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P31503 (PO2F1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 2, transcription factor 1
Alternative name(s):
NF-A1
Octamer-binding protein 1
Short name=Oct-1
Octamer-binding transcription factor 1
Short name=OTF-1
Gene names
Name:Pou2f1
Synonyms:Oct-1, Otf-1, Otf1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length632 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR By similarity.

Subunit structure

Interacts with NR3C1, AR, PGR and HCFC1 By similarity.

Subcellular location

Nucleus.

Tissue specificity

Widely expressed. Ref.2

Post-translational modification

Phosphorylated by PRKDC By similarity.

Sequence similarities

Belongs to the POU transcription factor family. Class-2 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 632›632POU domain, class 2, transcription factor 1
PRO_0000100711

Regions

Domain167 – 24175POU-specific
DNA binding268 – 32760Homeobox

Amino acid modifications

Modified residue1571Phosphothreonine By similarity
Modified residue1701Phosphoserine By similarity
Modified residue2741Phosphoserine By similarity
Modified residue3371Phosphoserine By similarity

Experimental info

Sequence conflict2181A → S in CAA42059. Ref.3
Sequence conflict2351K → S in CAA42059. Ref.3
Sequence conflict2511S → L in CAA42059. Ref.3
Sequence conflict2691R → S in CAA42059. Ref.3
Sequence conflict281 – 2822VA → WS in CAA42059. Ref.3
Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P31503 [UniParc].

Last modified February 1, 2003. Version 2.
Checksum: D8CEA4C27346274B

FASTA63265,170
        10         20         30         40         50         60 
LTPAQQQLLL QQAQAQAQLL AAAVQQHSAS QQHSAAGATI SASAATPMTQ IPLSQPIQIA 

        70         80         90        100        110        120 
QDLQQLQQLQ QQNLNLQQFV LVHPTTNLQP AQFIISQTPQ GQQGLLQAQN LLTQLPQQSQ 

       130        140        150        160        170        180 
ANLLQPQPSI TLTSQPTTPT RTIAATPIQT LPQSQTTPKR IDTPSLEEPS DLEELEQFAK 

       190        200        210        220        230        240 
TFKQRRIKLG FTQGDVGLAM GKLYGNDFSQ TTISRFEALN LSFKNMCKLK PLLEKWLNDA 

       250        260        270        280        290        300 
ENLSSDSTAS SPSALNSPGL GAEGLNRRRK KRTSIETNIR VALEKSFMEN QKPTSEDITL 

       310        320        330        340        350        360 
IAEQLNMEKE VIRVWFCNRR QKEKRINPPS SGGTSSSPIK AIFPSPTSLV ATTPSLVTSS 

       370        380        390        400        410        420 
TATTLTVNPV LPLTSAAMTN LSLTGTTDST SNNTATVIST APPASSAVTS PSLSPSPSAS 

       430        440        450        460        470        480 
ASTSEASSAS ETSTTQTTST PLPSPLGASQ VMVTASGLQT AAAAALQGAA QLPANASLAA 

       490        500        510        520        530        540 
MAAAAGLNPG LMAPSQFAAG GALLSLNPGT LGGALSPALM SNSTLATIQA LASSGSLPIT 

       550        560        570        580        590        600 
SLDATGNLVF ANAGGAPNIV TAPLFLNPQN LSLLTSNPVS LVSAAAASTG NSAPTASLHA 

       610        620        630 
SSTSTESIQN SLFTVASASG AASTTTAASK AQ 

« Hide

References

[1]"Identification of a muscle creatine kinase enhancer A/T-rich site binding factor as Oct-1."
Amacher S.L., Buskin J.N., Hauschka S.D.
Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Heart muscle.
[2]"The POU-domain protein Oct-1 is widely expressed in adult rat organs."
Kambe F., Tsukahara S., Kato T., Seo H.
Biochim. Biophys. Acta 1171:307-310(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 172-325, TISSUE SPECIFICITY.
[3]"Cloning and sequencing of the rat Oct-1 POU box."
Lillycrop K.A., Latchman D.S.
Nucleic Acids Res. 19:3744-3744(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 194-313.
Tissue: Spinal ganglion.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U17013 mRNA. Translation: AAA53185.1.
D12978 mRNA. Translation: BAA02355.1.
X59429 mRNA. Translation: CAA42059.1.
PIRS16445.
S28181.
RefSeqNP_001094109.1. NM_001100639.1.
UniGeneRn.46306.

3D structure databases

ProteinModelPortalP31503.
SMRP31503. Positions 168-328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000004937.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID171068.
KEGGrno:171068.
UCSCRGD:621689. rat.

Organism-specific databases

CTD5451.
RGD621689. Pou2f1.

Phylogenomic databases

eggNOGNOG267922.
HOGENOMHOG000220842.
HOVERGENHBG057016.
KOK09364.

Gene expression databases

GenevestigatorP31503.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR000972. TF_octamer.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSPR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio621657.

Entry information

Entry namePO2F1_RAT
AccessionPrimary (citable) accession number: P31503
Secondary accession number(s): Q62719, Q63387
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 2003
Last modified: April 16, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families