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P31503

- PO2F1_RAT

UniProt

P31503 - PO2F1_RAT

Protein

POU domain, class 2, transcription factor 1

Gene

Pou2f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (01 Feb 2003)
      Previous versions | rss
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    Functioni

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi268 – 32760HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: RGD
    2. sequence-specific DNA binding Source: Ensembl

    GO - Biological processi

    1. lens induction in camera-type eye Source: Ensembl
    2. negative regulation of transcription, DNA-templated Source: Ensembl
    3. olfactory placode formation Source: Ensembl

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 2, transcription factor 1
    Alternative name(s):
    NF-A1
    Octamer-binding protein 1
    Short name:
    Oct-1
    Octamer-binding transcription factor 1
    Short name:
    OTF-1
    Gene namesi
    Name:Pou2f1
    Synonyms:Oct-1, Otf-1, Otf1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621689. Pou2f1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: RGD
    2. transcription factor complex Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 632›632POU domain, class 2, transcription factor 1PRO_0000100711Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei157 – 1571PhosphothreonineBy similarity
    Modified residuei170 – 1701PhosphoserineBy similarity
    Modified residuei274 – 2741PhosphoserineBy similarity
    Modified residuei337 – 3371PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by PRKDC.By similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    GenevestigatoriP31503.

    Interactioni

    Subunit structurei

    Interacts with NR3C1, AR, PGR and HCFC1.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000004937.

    Structurei

    3D structure databases

    ProteinModelPortaliP31503.
    SMRiP31503. Positions 168-328.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini167 – 24175POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG267922.
    HOGENOMiHOG000220842.
    HOVERGENiHBG057016.
    KOiK09364.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    IPR000972. TF_octamer.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PRINTSiPR00029. OCTAMER.
    PR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    P31503-1 [UniParc]FASTAAdd to Basket

    « Hide

    LTPAQQQLLL QQAQAQAQLL AAAVQQHSAS QQHSAAGATI SASAATPMTQ    50
    IPLSQPIQIA QDLQQLQQLQ QQNLNLQQFV LVHPTTNLQP AQFIISQTPQ 100
    GQQGLLQAQN LLTQLPQQSQ ANLLQPQPSI TLTSQPTTPT RTIAATPIQT 150
    LPQSQTTPKR IDTPSLEEPS DLEELEQFAK TFKQRRIKLG FTQGDVGLAM 200
    GKLYGNDFSQ TTISRFEALN LSFKNMCKLK PLLEKWLNDA ENLSSDSTAS 250
    SPSALNSPGL GAEGLNRRRK KRTSIETNIR VALEKSFMEN QKPTSEDITL 300
    IAEQLNMEKE VIRVWFCNRR QKEKRINPPS SGGTSSSPIK AIFPSPTSLV 350
    ATTPSLVTSS TATTLTVNPV LPLTSAAMTN LSLTGTTDST SNNTATVIST 400
    APPASSAVTS PSLSPSPSAS ASTSEASSAS ETSTTQTTST PLPSPLGASQ 450
    VMVTASGLQT AAAAALQGAA QLPANASLAA MAAAAGLNPG LMAPSQFAAG 500
    GALLSLNPGT LGGALSPALM SNSTLATIQA LASSGSLPIT SLDATGNLVF 550
    ANAGGAPNIV TAPLFLNPQN LSLLTSNPVS LVSAAAASTG NSAPTASLHA 600
    SSTSTESIQN SLFTVASASG AASTTTAASK AQ 632
    Length:632
    Mass (Da):65,170
    Last modified:February 1, 2003 - v2
    Checksum:iD8CEA4C27346274B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Sequence conflicti218 – 2181A → S in CAA42059. (PubMed:1677182)Curated
    Sequence conflicti235 – 2351K → S in CAA42059. (PubMed:1677182)Curated
    Sequence conflicti251 – 2511S → L in CAA42059. (PubMed:1677182)Curated
    Sequence conflicti269 – 2691R → S in CAA42059. (PubMed:1677182)Curated
    Sequence conflicti281 – 2822VA → WS in CAA42059. (PubMed:1677182)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17013 mRNA. Translation: AAA53185.1.
    D12978 mRNA. Translation: BAA02355.1.
    X59429 mRNA. Translation: CAA42059.1.
    PIRiS16445.
    S28181.
    RefSeqiNP_001094109.1. NM_001100639.1.
    UniGeneiRn.46306.

    Genome annotation databases

    GeneIDi171068.
    KEGGirno:171068.
    UCSCiRGD:621689. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17013 mRNA. Translation: AAA53185.1 .
    D12978 mRNA. Translation: BAA02355.1 .
    X59429 mRNA. Translation: CAA42059.1 .
    PIRi S16445.
    S28181.
    RefSeqi NP_001094109.1. NM_001100639.1.
    UniGenei Rn.46306.

    3D structure databases

    ProteinModelPortali P31503.
    SMRi P31503. Positions 168-328.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000004937.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 171068.
    KEGGi rno:171068.
    UCSCi RGD:621689. rat.

    Organism-specific databases

    CTDi 5451.
    RGDi 621689. Pou2f1.

    Phylogenomic databases

    eggNOGi NOG267922.
    HOGENOMi HOG000220842.
    HOVERGENi HBG057016.
    KOi K09364.

    Miscellaneous databases

    NextBioi 621657.

    Gene expression databases

    Genevestigatori P31503.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    IPR000972. TF_octamer.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PRINTSi PR00029. OCTAMER.
    PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a muscle creatine kinase enhancer A/T-rich site binding factor as Oct-1."
      Amacher S.L., Buskin J.N., Hauschka S.D.
      Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Heart muscle.
    2. "The POU-domain protein Oct-1 is widely expressed in adult rat organs."
      Kambe F., Tsukahara S., Kato T., Seo H.
      Biochim. Biophys. Acta 1171:307-310(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 172-325, TISSUE SPECIFICITY.
    3. "Cloning and sequencing of the rat Oct-1 POU box."
      Lillycrop K.A., Latchman D.S.
      Nucleic Acids Res. 19:3744-3744(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 194-313.
      Tissue: Spinal ganglion.

    Entry informationi

    Entry nameiPO2F1_RAT
    AccessioniPrimary (citable) accession number: P31503
    Secondary accession number(s): Q62719, Q63387
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: February 1, 2003
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3