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Protein

POU domain, class 2, transcription factor 1

Gene

Pou2f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi268 – 327HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • chromatin binding Source: RGD
  • receptor binding Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: InterPro
  • transcription regulatory region sequence-specific DNA binding Source: RGD

GO - Biological processi

  • liver regeneration Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 2, transcription factor 1
Alternative name(s):
NF-A1
Octamer-binding protein 1
Short name:
Oct-1
Octamer-binding transcription factor 1
Short name:
OTF-1
Gene namesi
Name:Pou2f1
Synonyms:Oct-1, Otf-1, Otf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621689. Pou2f1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000100711‹1 – 632POU domain, class 2, transcription factor 1Add BLAST›632

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157PhosphothreonineBy similarity1
Modified residuei163PhosphothreonineBy similarity1
Modified residuei170PhosphoserineBy similarity1
Modified residuei274PhosphoserineBy similarity1
Modified residuei337PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by PRKDC.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP31503.
PeptideAtlasiP31503.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Interactioni

Subunit structurei

Interacts with NR3C1, AR, PGR and HCFC1.By similarity

GO - Molecular functioni

  • receptor binding Source: RGD

Protein-protein interaction databases

CORUMiP31503.
STRINGi10116.ENSRNOP00000044416.

Structurei

3D structure databases

ProteinModelPortaliP31503.
SMRiP31503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini167 – 241POU-specificPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
HOGENOMiHOG000220842.
HOVERGENiHBG057016.
InParanoidiP31503.
KOiK09364.

Family and domain databases

CDDicd00086. homeodomain. 1 hit.
InterProiView protein in InterPro
IPR009057. Homeobox-like.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
IPR000972. TF_octamer.
PfamiView protein in Pfam
PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
PRINTSiPR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTiView protein in SMART
SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiView protein in PROSITE
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.

Sequencei

Sequence statusi: Fragment.

P31503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
LTPAQQQLLL QQAQAQAQLL AAAVQQHSAS QQHSAAGATI SASAATPMTQ
60 70 80 90 100
IPLSQPIQIA QDLQQLQQLQ QQNLNLQQFV LVHPTTNLQP AQFIISQTPQ
110 120 130 140 150
GQQGLLQAQN LLTQLPQQSQ ANLLQPQPSI TLTSQPTTPT RTIAATPIQT
160 170 180 190 200
LPQSQTTPKR IDTPSLEEPS DLEELEQFAK TFKQRRIKLG FTQGDVGLAM
210 220 230 240 250
GKLYGNDFSQ TTISRFEALN LSFKNMCKLK PLLEKWLNDA ENLSSDSTAS
260 270 280 290 300
SPSALNSPGL GAEGLNRRRK KRTSIETNIR VALEKSFMEN QKPTSEDITL
310 320 330 340 350
IAEQLNMEKE VIRVWFCNRR QKEKRINPPS SGGTSSSPIK AIFPSPTSLV
360 370 380 390 400
ATTPSLVTSS TATTLTVNPV LPLTSAAMTN LSLTGTTDST SNNTATVIST
410 420 430 440 450
APPASSAVTS PSLSPSPSAS ASTSEASSAS ETSTTQTTST PLPSPLGASQ
460 470 480 490 500
VMVTASGLQT AAAAALQGAA QLPANASLAA MAAAAGLNPG LMAPSQFAAG
510 520 530 540 550
GALLSLNPGT LGGALSPALM SNSTLATIQA LASSGSLPIT SLDATGNLVF
560 570 580 590 600
ANAGGAPNIV TAPLFLNPQN LSLLTSNPVS LVSAAAASTG NSAPTASLHA
610 620 630
SSTSTESIQN SLFTVASASG AASTTTAASK AQ
Length:632
Mass (Da):65,170
Last modified:February 1, 2003 - v2
Checksum:iD8CEA4C27346274B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Sequence conflicti218A → S in CAA42059 (PubMed:1677182).Curated1
Sequence conflicti235K → S in CAA42059 (PubMed:1677182).Curated1
Sequence conflicti251S → L in CAA42059 (PubMed:1677182).Curated1
Sequence conflicti269R → S in CAA42059 (PubMed:1677182).Curated1
Sequence conflicti281 – 282VA → WS in CAA42059 (PubMed:1677182).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17013 mRNA. Translation: AAA53185.1.
D12978 mRNA. Translation: BAA02355.1.
X59429 mRNA. Translation: CAA42059.1.
PIRiS16445.
S28181.
RefSeqiNP_001094109.1. NM_001100639.1.
UniGeneiRn.46306.

Genome annotation databases

GeneIDi171068.
KEGGirno:171068.
UCSCiRGD:621689. rat.

Similar proteinsi

Entry informationi

Entry nameiPO2F1_RAT
AccessioniPrimary (citable) accession number: P31503
Secondary accession number(s): Q62719, Q63387
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 2003
Last modified: September 27, 2017
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families