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P31503

- PO2F1_RAT

UniProt

P31503 - PO2F1_RAT

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Protein

POU domain, class 2, transcription factor 1

Gene

Pou2f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR By similarity.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi268 – 32760HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: RGD
  2. sequence-specific DNA binding Source: Ensembl

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: Ensembl
  2. positive regulation of transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 2, transcription factor 1
Alternative name(s):
NF-A1
Octamer-binding protein 1
Short name:
Oct-1
Octamer-binding transcription factor 1
Short name:
OTF-1
Gene namesi
Name:Pou2f1
Synonyms:Oct-1, Otf-1, Otf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621689. Pou2f1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 632›632POU domain, class 2, transcription factor 1PRO_0000100711Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei157 – 1571PhosphothreonineBy similarity
Modified residuei170 – 1701PhosphoserineBy similarity
Modified residuei274 – 2741PhosphoserineBy similarity
Modified residuei337 – 3371PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by PRKDC.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

ExpressionAtlasiP31503. baseline.
GenevestigatoriP31503.

Interactioni

Subunit structurei

Interacts with NR3C1, AR, PGR and HCFC1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004937.

Structurei

3D structure databases

ProteinModelPortaliP31503.
SMRiP31503. Positions 168-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini167 – 24175POU-specificPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG267922.
HOGENOMiHOG000220842.
HOVERGENiHBG057016.
InParanoidiP31503.
KOiK09364.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR000972. TF_octamer.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P31503-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
LTPAQQQLLL QQAQAQAQLL AAAVQQHSAS QQHSAAGATI SASAATPMTQ
60 70 80 90 100
IPLSQPIQIA QDLQQLQQLQ QQNLNLQQFV LVHPTTNLQP AQFIISQTPQ
110 120 130 140 150
GQQGLLQAQN LLTQLPQQSQ ANLLQPQPSI TLTSQPTTPT RTIAATPIQT
160 170 180 190 200
LPQSQTTPKR IDTPSLEEPS DLEELEQFAK TFKQRRIKLG FTQGDVGLAM
210 220 230 240 250
GKLYGNDFSQ TTISRFEALN LSFKNMCKLK PLLEKWLNDA ENLSSDSTAS
260 270 280 290 300
SPSALNSPGL GAEGLNRRRK KRTSIETNIR VALEKSFMEN QKPTSEDITL
310 320 330 340 350
IAEQLNMEKE VIRVWFCNRR QKEKRINPPS SGGTSSSPIK AIFPSPTSLV
360 370 380 390 400
ATTPSLVTSS TATTLTVNPV LPLTSAAMTN LSLTGTTDST SNNTATVIST
410 420 430 440 450
APPASSAVTS PSLSPSPSAS ASTSEASSAS ETSTTQTTST PLPSPLGASQ
460 470 480 490 500
VMVTASGLQT AAAAALQGAA QLPANASLAA MAAAAGLNPG LMAPSQFAAG
510 520 530 540 550
GALLSLNPGT LGGALSPALM SNSTLATIQA LASSGSLPIT SLDATGNLVF
560 570 580 590 600
ANAGGAPNIV TAPLFLNPQN LSLLTSNPVS LVSAAAASTG NSAPTASLHA
610 620 630
SSTSTESIQN SLFTVASASG AASTTTAASK AQ
Length:632
Mass (Da):65,170
Last modified:February 1, 2003 - v2
Checksum:iD8CEA4C27346274B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Sequence conflicti218 – 2181A → S in CAA42059. (PubMed:1677182)Curated
Sequence conflicti235 – 2351K → S in CAA42059. (PubMed:1677182)Curated
Sequence conflicti251 – 2511S → L in CAA42059. (PubMed:1677182)Curated
Sequence conflicti269 – 2691R → S in CAA42059. (PubMed:1677182)Curated
Sequence conflicti281 – 2822VA → WS in CAA42059. (PubMed:1677182)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17013 mRNA. Translation: AAA53185.1.
D12978 mRNA. Translation: BAA02355.1.
X59429 mRNA. Translation: CAA42059.1.
PIRiS16445.
S28181.
RefSeqiNP_001094109.1. NM_001100639.1.
UniGeneiRn.46306.

Genome annotation databases

GeneIDi171068.
KEGGirno:171068.
UCSCiRGD:621689. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17013 mRNA. Translation: AAA53185.1 .
D12978 mRNA. Translation: BAA02355.1 .
X59429 mRNA. Translation: CAA42059.1 .
PIRi S16445.
S28181.
RefSeqi NP_001094109.1. NM_001100639.1.
UniGenei Rn.46306.

3D structure databases

ProteinModelPortali P31503.
SMRi P31503. Positions 168-328.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000004937.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 171068.
KEGGi rno:171068.
UCSCi RGD:621689. rat.

Organism-specific databases

CTDi 5451.
RGDi 621689. Pou2f1.

Phylogenomic databases

eggNOGi NOG267922.
HOGENOMi HOG000220842.
HOVERGENi HBG057016.
InParanoidi P31503.
KOi K09364.

Miscellaneous databases

NextBioi 621657.

Gene expression databases

ExpressionAtlasi P31503. baseline.
Genevestigatori P31503.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR000972. TF_octamer.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00029. OCTAMER.
PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of a muscle creatine kinase enhancer A/T-rich site binding factor as Oct-1."
    Amacher S.L., Buskin J.N., Hauschka S.D.
    Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Heart muscle.
  2. "The POU-domain protein Oct-1 is widely expressed in adult rat organs."
    Kambe F., Tsukahara S., Kato T., Seo H.
    Biochim. Biophys. Acta 1171:307-310(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 172-325, TISSUE SPECIFICITY.
  3. "Cloning and sequencing of the rat Oct-1 POU box."
    Lillycrop K.A., Latchman D.S.
    Nucleic Acids Res. 19:3744-3744(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 194-313.
    Tissue: Spinal ganglion.

Entry informationi

Entry nameiPO2F1_RAT
AccessioniPrimary (citable) accession number: P31503
Secondary accession number(s): Q62719, Q63387
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 2003
Last modified: October 29, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3