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Protein

POU domain, class 2, transcription factor 1

Gene

Pou2f1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi268 – 327HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • chromatin binding Source: RGD
  • DNA binding transcription factor activity Source: InterPro
  • transcription regulatory region sequence-specific DNA binding Source: RGD

GO - Biological processi

  • liver regeneration Source: RGD
  • negative regulation of gene expression Source: BHF-UCL
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 2, transcription factor 1
Alternative name(s):
NF-A1
Octamer-binding protein 1
Short name:
Oct-1
Octamer-binding transcription factor 1
Short name:
OTF-1
Gene namesi
Name:Pou2f1
Synonyms:Oct-1, Otf-1, Otf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621689 Pou2f1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000100711‹1 – 632POU domain, class 2, transcription factor 1Add BLAST›632

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157PhosphothreonineBy similarity1
Modified residuei163PhosphothreonineBy similarity1
Modified residuei170PhosphoserineBy similarity1
Modified residuei274PhosphoserineBy similarity1
Modified residuei337PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by PRKDC.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP31503
PeptideAtlasiP31503
PRIDEiP31503

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Interactioni

Subunit structurei

Interacts with NR3C1, AR, PGR and HCFC1.By similarity

Protein-protein interaction databases

CORUMiP31503
STRINGi10116.ENSRNOP00000044416

Structurei

3D structure databases

ProteinModelPortaliP31503
SMRiP31503
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini167 – 241POU-specificPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802 Eukaryota
ENOG410XQ7X LUCA
HOGENOMiHOG000220842
HOVERGENiHBG057016
InParanoidiP31503
KOiK09364

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR013847 POU
IPR000327 POU_dom
IPR000972 TF_octamer
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00157 Pou, 1 hit
PRINTSiPR00029 OCTAMER
PR00028 POUDOMAIN
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00352 POU, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00035 POU_1, 1 hit
PS00465 POU_2, 1 hit
PS51179 POU_3, 1 hit

Sequencei

Sequence statusi: Fragment.

P31503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
LTPAQQQLLL QQAQAQAQLL AAAVQQHSAS QQHSAAGATI SASAATPMTQ
60 70 80 90 100
IPLSQPIQIA QDLQQLQQLQ QQNLNLQQFV LVHPTTNLQP AQFIISQTPQ
110 120 130 140 150
GQQGLLQAQN LLTQLPQQSQ ANLLQPQPSI TLTSQPTTPT RTIAATPIQT
160 170 180 190 200
LPQSQTTPKR IDTPSLEEPS DLEELEQFAK TFKQRRIKLG FTQGDVGLAM
210 220 230 240 250
GKLYGNDFSQ TTISRFEALN LSFKNMCKLK PLLEKWLNDA ENLSSDSTAS
260 270 280 290 300
SPSALNSPGL GAEGLNRRRK KRTSIETNIR VALEKSFMEN QKPTSEDITL
310 320 330 340 350
IAEQLNMEKE VIRVWFCNRR QKEKRINPPS SGGTSSSPIK AIFPSPTSLV
360 370 380 390 400
ATTPSLVTSS TATTLTVNPV LPLTSAAMTN LSLTGTTDST SNNTATVIST
410 420 430 440 450
APPASSAVTS PSLSPSPSAS ASTSEASSAS ETSTTQTTST PLPSPLGASQ
460 470 480 490 500
VMVTASGLQT AAAAALQGAA QLPANASLAA MAAAAGLNPG LMAPSQFAAG
510 520 530 540 550
GALLSLNPGT LGGALSPALM SNSTLATIQA LASSGSLPIT SLDATGNLVF
560 570 580 590 600
ANAGGAPNIV TAPLFLNPQN LSLLTSNPVS LVSAAAASTG NSAPTASLHA
610 620 630
SSTSTESIQN SLFTVASASG AASTTTAASK AQ
Length:632
Mass (Da):65,170
Last modified:February 1, 2003 - v2
Checksum:iD8CEA4C27346274B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Sequence conflicti218A → S in CAA42059 (PubMed:1677182).Curated1
Sequence conflicti235K → S in CAA42059 (PubMed:1677182).Curated1
Sequence conflicti251S → L in CAA42059 (PubMed:1677182).Curated1
Sequence conflicti269R → S in CAA42059 (PubMed:1677182).Curated1
Sequence conflicti281 – 282VA → WS in CAA42059 (PubMed:1677182).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17013 mRNA Translation: AAA53185.1
D12978 mRNA Translation: BAA02355.1
X59429 mRNA Translation: CAA42059.1
PIRiS16445
S28181
RefSeqiNP_001094109.1, NM_001100639.1
UniGeneiRn.46306

Genome annotation databases

GeneIDi171068
KEGGirno:171068
UCSCiRGD:621689 rat

Similar proteinsi

Entry informationi

Entry nameiPO2F1_RAT
AccessioniPrimary (citable) accession number: P31503
Secondary accession number(s): Q62719, Q63387
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 1, 2003
Last modified: May 23, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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