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Protein

Urease subunit alpha

Gene

ureC

Organism
Yersinia enterocolitica
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Expression of the urease operon increases the likelihood of bacterial survival by contibuting to acid resistance in vitro and in vivo in BALB/c mice. Y.enterocolitica enters the body via an oral path and must survive the acidic stomach before being able to colonize the intestinal mucosa (PubMed:7558281).1 Publication

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit alpha (ureC), Urease subunit alpha (ureC), Urease subunit gamma (ureA), Urease subunit beta (ureB), Urease subunit alpha (ureC), Urease subunit beta (ureB), Urease subunit alpha (ureC), Urease subunit beta (ureB), Urease subunit beta (ureB), Urease subunit beta (ureB), Urease subunit alpha (ureC), Urease subunit alpha (ureC), Urease subunit beta (ureB), Urease subunit alpha (ureC), Urease subunit gamma (ureA)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi139 – 1391Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi141 – 1411Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi222 – 2221Nickel 1; via carbamate groupUniRule annotation
Metal bindingi222 – 2221Nickel 2; via carbamate groupUniRule annotation
Binding sitei224 – 2241SubstrateUniRule annotation
Metal bindingi251 – 2511Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi277 – 2771Nickel 2; via tele nitrogenUniRule annotation
Active sitei325 – 3251Proton donorUniRule annotation
Metal bindingi365 – 3651Nickel 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Virulence

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Synonyms:yeuC
OrganismiYersinia enterocolitica
Taxonomic identifieri630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Urease subunit alphaPRO_0000067565Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei222 – 2221N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi393305.YE0953.

Structurei

3D structure databases

ProteinModelPortaliP31494.
SMRiP31494. Positions 3-571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini134 – 572439UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31494-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQISRQEYA GLFGPTTGDK IRLGDTNLFI EIEKDLRGYG EESVYGGGKS
60 70 80 90 100
LRDGMGANNH LTRDNGVLDL VITNVTIVDA RLGVIKADVG IRDGKIAGIG
110 120 130 140 150
KSGNPGVMDG VTPGMVVGVS TDAISGEHLI LTAAGIDSHI HLISPQQAYH
160 170 180 190 200
ALSNGVATFF GGGIGPTDGT NGTTVTPGPW NIRQMLRSVE GLPVNVGILG
210 220 230 240 250
KGNSYGRGPL LEQAIAGVVG YKVHEDWGAT ANALRHSLRM ADEMDIQVSV
260 270 280 290 300
HTDSLNECGY VEDTIDAFEG RTIHTFHTEG AGGGHAPDII RVASQPNVLP
310 320 330 340 350
SSTNPTLPYG VNSQAELFDM IMVCHNLNPN VPADVSFAES RVRPETIAAE
360 370 380 390 400
NVLHDMGVIS MFSSDSQAMG RVGENWLRVM QTANAMKASR GKLPEDAPGN
410 420 430 440 450
DNFRVLRYVA KITINPAIAQ GVSHVIGSVE VGKMADLVLW DPRFFGAKPK
460 470 480 490 500
MVIKGGMINW AAMGDPNASL PTPQPVFYRP MFGAMGKTMQ DTCVTFVSQA
510 520 530 540 550
ALDDGVKEKA GLDRQVIAVK NCRTISKHDL VRNDQTPNIE VDPETFAVKV
560 570
DGVHATCEPI DTAAMNQRYF FG
Length:572
Mass (Da):61,083
Last modified:May 2, 2006 - v3
Checksum:iB9A36459DDC3E963
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 312IE → MQ in CAA79316 (PubMed:8500886).Curated
Sequence conflicti67 – 671V → GY (PubMed:8500886).Curated
Sequence conflicti69 – 8315DLVIT…DARLG → EFSHNQRHYCCSPFR (PubMed:8500886).CuratedAdd
BLAST
Sequence conflicti97 – 971A → V in CAA79316 (PubMed:8500886).Curated
Sequence conflicti115 – 1151M → L in CAA79316 (PubMed:8500886).Curated
Sequence conflicti138 – 1381S → T in CAA79316 (PubMed:8500886).Curated
Sequence conflicti406 – 4061L → V in CAA79316 (PubMed:8500886).Curated
Sequence conflicti441 – 4411D → V in CAA79316 (PubMed:8500886).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18865 Genomic DNA. Translation: CAA79316.1.
L24101 Genomic DNA. Translation: AAA50996.1.
PIRiS36028.
RefSeqiWP_011815716.1. NZ_KN150733.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18865 Genomic DNA. Translation: CAA79316.1.
L24101 Genomic DNA. Translation: AAA50996.1.
PIRiS36028.
RefSeqiWP_011815716.1. NZ_KN150733.1.

3D structure databases

ProteinModelPortaliP31494.
SMRiP31494. Positions 3-571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi393305.YE0953.

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The putative arthritogenic cationic 19-kilodalton antigen of Yersinia enterocolitica is a urease beta-subunit."
    Skurnik M., Batsford S., Mertz A.K.H., Schiltz E., Toivanen P.
    Infect. Immun. 61:2498-2504(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 6471/76 / Serotype O:3.
  2. "Contribution of urease to acid tolerance in Yersinia enterocolitica."
    de Koning-Ward T.F., Robins-Browne R.M.
    Infect. Immun. 63:3790-3795(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN VIRULENCE.
    Strain: W22703 / Serogroup O:9.
  3. "Characterisation of the urease-encoding gene complex of Yersinia enterocolitica."
    de Koning-Ward T.F., Ward A.C., Robins-Browne R.M.
    Gene 145:25-32(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A2635 / Serotype O:8.

Entry informationi

Entry nameiURE1_YEREN
AccessioniPrimary (citable) accession number: P31494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 2, 2006
Last modified: November 11, 2015
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.