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Protein

Adhesin YadA

Gene

yadA

Organism
Yersinia enterocolitica
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Collagen-binding outer membrane protein forming a fibrillar matrix on the bacterial cell surface. Promotes initial attachment and invasion of eukaryotic cells. Also protects the bacteria by being responsible for agglutination, serum resistance, complement inactivation and phagocytosis resistance.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesin YadA
Gene namesi
Name:yadA
Synonyms:invA, yop1, yopA
Encoded oniPlasmid pYV6471/765 Publications
Plasmid pYV1 Publication
Plasmid pYVe2271 Publication
OrganismiYersinia enterocolitica
Taxonomic identifieri630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi73 – 753IAI → EDE: 8% of wild-type collagen-binding activity. 1 Publication
Mutagenesisi101 – 1033VAI → DDE: 3% of wild-type collagen-binding activity. 1 Publication
Mutagenesisi155 – 1573VAI → DDE: Loss of collagen-binding activity. 1 Publication
Mutagenesisi155 – 1573VAI → QST: Less than 0.5% of wild-type collagen-binding activity. 1 Publication
Mutagenesisi155 – 1551V → D: Less than 1% of wild-type collagen-binding activity. 1 Publication
Mutagenesisi156 – 1561A → D: Loss of collagen-binding activity. 1 Publication
Mutagenesisi171 – 1733IAI → EDE: Loss of collagen-binding activity. 1 Publication
Mutagenesisi185 – 1873VSI → DDE: Loss of collagen-binding activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Add
BLAST
Chaini26 – 455430Adhesin YadAPRO_0000022699Add
BLAST

Expressioni

Inductioni

Induced at 37 degrees Celsius by the temperature-dependent transcriptional activator LcrF (VirF).1 Publication

Interactioni

Subunit structurei

Homotrimer.

Structurei

Secondary structure

1
455
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi37 – 393Combined sources
Turni45 – 473Combined sources
Beta strandi73 – 764Combined sources
Beta strandi87 – 904Combined sources
Beta strandi101 – 1044Combined sources
Beta strandi115 – 1184Combined sources
Beta strandi128 – 1314Combined sources
Beta strandi141 – 1444Combined sources
Beta strandi155 – 1584Combined sources
Beta strandi171 – 1744Combined sources
Beta strandi181 – 1833Combined sources
Beta strandi185 – 1873Combined sources
Helixi211 – 2199Combined sources
Helixi335 – 38551Combined sources
Helixi387 – 3948Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1P9HX-ray1.55A26-241[»]
3LT6X-ray1.80A/B/C/D/E/F333-396[»]
3LT7X-ray1.50A/B/C/D/E/F333-396[»]
ProteinModelPortaliP31489.
SMRiP31489. Positions 32-220.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31489.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili209 – 24335Sequence AnalysisAdd
BLAST

Domaini

Presents a lollipop-shaped form which consists of three domains: a C-terminal membrane-anchor domain, a coiled-coil stalk domain and an oval N-terminal head domain. The N-terminal half of the sequence reveals the presence of repeated motifs with the consensus sequence NSVAIGXXS. They form the turns and hydrophobic core of the nine-coiled left-handed parallel beta-roll and trimer structure essential for the collagen-binding activity.1 Publication

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

KOiK12341.

Family and domain databases

Gene3Di2.150.10.10. 1 hit.
InterProiIPR008640. Adhesin_Head_dom.
IPR008635. Coiled_stalk_dom.
IPR008126. OM_adhesion_Yersinia.
IPR011049. Serralysin-like_metalloprot_C.
IPR005594. YadA_C.
[Graphical view]
PfamiPF03895. YadA_anchor. 1 hit.
PF05658. YadA_head. 2 hits.
PF05662. YadA_stalk. 1 hit.
[Graphical view]
PRINTSiPR01756. OMADHESIN.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31489-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKDFKISVS AALISALFSS PYAFADDYDG IPNLTAVQIS PNADPALGLE
60 70 80 90 100
YPVRPPVPGA GGLNASAKGI HSIAIGATAE AAKGAAVAVG AGSIATGVNS
110 120 130 140 150
VAIGPLSKAL GDSAVTYGAA STAQKDGVAI GARASTSDTG VAVGFNSKAD
160 170 180 190 200
AKNSVAIGHS SHVAANHGYS IAIGDRSKTD RENSVSIGHE SLNRQLTHLA
210 220 230 240 250
AGTKDTDAVN VAQLKKEIEK TQENTNKRSA ELLANANAYA DNKSSSVLGI
260 270 280 290 300
ANNYTDSKSA ETLENARKEA FAQSKDVLNM AKAHSNSVAR TTLETAEEHA
310 320 330 340 350
NSVARTTLET AEEHANKKSA EALASANVYA DSKSSHTLKT ANSYTDVTVS
360 370 380 390 400
NSTKKAIRES NQYTDHKFRQ LDNRLDKLDT RVDKGLASSA ALNSLFQPYG
410 420 430 440 450
VGKVNFTAGV GGYRSSQALA IGSGYRVNEN VALKAGVAYA GSSDVMYNAS

FNIEW
Length:455
Mass (Da):47,136
Last modified:July 1, 1993 - v1
Checksum:iAC12EF68C657DAC0
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti29 – 291D → N in strain: Serotype O:9.
Natural varianti49 – 491L → Q in strain: Serotype O:9.
Natural varianti65 – 651A → C in strain: Serotype O:9.
Natural varianti228 – 2281R → K in strain: Serotype O:9.
Natural varianti235 – 2362NA → KP in strain: Serotype O:9.
Natural varianti435 – 4406AGVAYA → PVWLI in strain: Serotype O:9.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13882 Genomic DNA. Translation: CAA32086.1.
AF056092 Genomic DNA. Translation: AAC33679.1.
AF102990 Genomic DNA. Translation: AAD16868.1.
PIRiS04912.
RefSeqiWP_032488477.1. NG_034465.1.
YP_009074088.1. NG_034465.1.

Genome annotation databases

KEGGipg:1239174.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13882 Genomic DNA. Translation: CAA32086.1.
AF056092 Genomic DNA. Translation: AAC33679.1.
AF102990 Genomic DNA. Translation: AAD16868.1.
PIRiS04912.
RefSeqiWP_032488477.1. NG_034465.1.
YP_009074088.1. NG_034465.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1P9HX-ray1.55A26-241[»]
3LT6X-ray1.80A/B/C/D/E/F333-396[»]
3LT7X-ray1.50A/B/C/D/E/F333-396[»]
ProteinModelPortaliP31489.
SMRiP31489. Positions 32-220.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGipg:1239174.

Phylogenomic databases

KOiK12341.

Miscellaneous databases

EvolutionaryTraceiP31489.

Family and domain databases

Gene3Di2.150.10.10. 1 hit.
InterProiIPR008640. Adhesin_Head_dom.
IPR008635. Coiled_stalk_dom.
IPR008126. OM_adhesion_Yersinia.
IPR011049. Serralysin-like_metalloprot_C.
IPR005594. YadA_C.
[Graphical view]
PfamiPF03895. YadA_anchor. 1 hit.
PF05658. YadA_head. 2 hits.
PF05662. YadA_stalk. 1 hit.
[Graphical view]
PRINTSiPR01756. OMADHESIN.
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the yopA gene encoding the Yop1 virulence determinants of Yersinia spp."
    Skurnik M., Wolf-Watz H.
    Mol. Microbiol. 3:517-529(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 6471/76 / Serotype O:3.
    Plasmid: pYV6471/76
  2. "Hydrophobic domains affect the collagen-binding specificity and surface polymerization as well as the virulence potential of the YadA protein of Yersinia enterocolitica."
    Tamm A., Tarkkanen A., Korhonen T.K., Kuusela P., Toivanen P., Skurnik M.
    Mol. Microbiol. 10:995-1011(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 6471/76 / Serotype O:3.
    Plasmid: pYV6471/76
  3. "YadA and ORF291 of Yersinia enterocolitica O:9."
    Iriarte M., Kerbourch C., Lambermont I., Cornelis G.R.
    Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Serotype O:9.
    Plasmid: pYV
  4. "Detailed genetic map of the pYVe227 plasmid of Yersinia enterocolitica serotype O:9."
    Iriarte M., Lambermont I., Kerbourch C., Cornelis G.R.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: W22703 / Serotype O:9 / Biotype 2.
    Plasmid: pYVe227
  5. "Binding to collagen by Yersinia enterocolitica and Yersinia pseudotuberculosis: evidence for yopA-mediated and chromosomally encoded mechanisms."
    Emoedy L., Heesemann J., Wolf-Watz H., Skurnik M., Kapperud G., O'Toole P., Wadstroem T.
    J. Bacteriol. 171:6674-6679(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: Various strains.
  6. "LcrF is the temperature-regulated activator of the yadA gene of Yersinia enterocolitica and Yersinia pseudotuberculosis."
    Skurnik M., Toivanen P.
    J. Bacteriol. 174:2047-2051(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: 6471/76 / Serotype O:3.
    Plasmid: pYV6471/76
  7. "Functional mapping of the Yersinia enterocolitica adhesin YadA. Identification of eight NSVAIG - S motifs in the amino-terminal half of the protein involved in collagen binding."
    Tahir Y.E., Kuusela P., Skurnik M.
    Mol. Microbiol. 37:192-206(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN COLLAGEN-BINDING, MUTAGENESIS OF 73-ILE--ILE-75; 101-VAL--ILE-103; 155-VAL--ILE-157; VAL-155; ALA-156; 171-ILE--ILE-173 AND 185-VAL--ILE-187.
    Strain: 6471/76 / Serotype O:3.
    Plasmid: pYV6471/76
  8. "Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA."
    Nummelin H., El Tahir Y., Ollikka P., Skurnik M., Goldman A.
    Acta Crystallogr. D 58:1042-1044(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
    Strain: 6471/76 / Serotype O:3.
    Plasmid: pYV6471/76
  9. "The Yersinia adhesin YadA collagen-binding domain structure is a novel left-handed parallel beta-roll."
    Nummelin H., Merckel M.C., Leo J.C., Lankinen H., Skurnik M., Goldman A.
    EMBO J. 23:701-711(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 26-241.
    Strain: 6471/76 / Serotype O:3.

Entry informationi

Entry nameiYADA1_YEREN
AccessioniPrimary (citable) accession number: P31489
Secondary accession number(s): O85267
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 22, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.