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P31489

- YADA1_YEREN

UniProt

P31489 - YADA1_YEREN

Protein

Adhesin YadA

Gene

yadA

Organism
Yersinia enterocolitica
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Collagen-binding outer membrane protein forming a fibrillar matrix on the bacterial cell surface. Promotes initial attachment and invasion of eukaryotic cells. Also protects the bacteria by being responsible for agglutination, serum resistance, complement inactivation and phagocytosis resistance.1 Publication

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. pathogenesis Source: UniProtKB-KW

    Keywords - Biological processi

    Cell adhesion, Virulence

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adhesin YadA
    Gene namesi
    Name:yadA
    Synonyms:invA, yop1, yopA
    Encoded oniPlasmid pYV6471/765 Publications
    Plasmid pYV1 Publication
    Plasmid pYVe2271 Publication
    OrganismiYersinia enterocolitica
    Taxonomic identifieri630 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

    Subcellular locationi

    GO - Cellular componenti

    1. cell outer membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell outer membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi73 – 753IAI → EDE: 8% of wild-type collagen-binding activity.
    Mutagenesisi101 – 1033VAI → DDE: 3% of wild-type collagen-binding activity.
    Mutagenesisi155 – 1573VAI → DDE: Loss of collagen-binding activity. 1 Publication
    Mutagenesisi155 – 1573VAI → QST: Less than 0.5% of wild-type collagen-binding activity. 1 Publication
    Mutagenesisi155 – 1551V → D: Less than 1% of wild-type collagen-binding activity. 1 Publication
    Mutagenesisi156 – 1561A → D: Loss of collagen-binding activity. 1 Publication
    Mutagenesisi171 – 1733IAI → EDE: Loss of collagen-binding activity.
    Mutagenesisi185 – 1873VSI → DDE: Loss of collagen-binding activity.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Add
    BLAST
    Chaini26 – 455430Adhesin YadAPRO_0000022699Add
    BLAST

    Expressioni

    Inductioni

    Induced at 37 degrees Celsius by the temperature-dependent transcriptional activator LcrF (VirF).1 Publication

    Interactioni

    Subunit structurei

    Homotrimer.

    Structurei

    Secondary structure

    1
    455
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi37 – 393
    Turni45 – 473
    Beta strandi73 – 764
    Beta strandi87 – 904
    Beta strandi101 – 1044
    Beta strandi115 – 1184
    Beta strandi128 – 1314
    Beta strandi141 – 1444
    Beta strandi155 – 1584
    Beta strandi171 – 1744
    Beta strandi181 – 1833
    Beta strandi185 – 1873
    Helixi211 – 2199
    Helixi335 – 38551

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1P9HX-ray1.55A26-241[»]
    3LT6X-ray1.80A/B/C/D/E/F333-396[»]
    3LT7X-ray1.50A/B/C/D/E/F333-396[»]
    ProteinModelPortaliP31489.
    SMRiP31489. Positions 32-220.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP31489.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili209 – 24335Sequence AnalysisAdd
    BLAST

    Domaini

    Presents a lollipop-shaped form which consists of three domains: a C-terminal membrane-anchor domain, a coiled-coil stalk domain and an oval N-terminal head domain. The N-terminal half of the sequence reveals the presence of repeated motifs with the consensus sequence NSVAIGXXS. They form the turns and hydrophobic core of the nine-coiled left-handed parallel beta-roll and trimer structure essential for the collagen-binding activity.1 Publication

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil, Signal, Transmembrane, Transmembrane beta strand

    Family and domain databases

    Gene3Di2.150.10.10. 1 hit.
    InterProiIPR008640. Adhesin_Head_dom.
    IPR008635. Coiled_stalk_dom.
    IPR008126. OM_adhesion_Yersinia.
    IPR011049. Serralysin-like_metalloprot_C.
    IPR005594. YadA_C.
    [Graphical view]
    PfamiPF03895. YadA_anchor. 1 hit.
    PF05658. YadA_head. 2 hits.
    PF05662. YadA_stalk. 1 hit.
    [Graphical view]
    PRINTSiPR01756. OMADHESIN.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31489-1 [UniParc]FASTAAdd to Basket

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    MTKDFKISVS AALISALFSS PYAFADDYDG IPNLTAVQIS PNADPALGLE    50
    YPVRPPVPGA GGLNASAKGI HSIAIGATAE AAKGAAVAVG AGSIATGVNS 100
    VAIGPLSKAL GDSAVTYGAA STAQKDGVAI GARASTSDTG VAVGFNSKAD 150
    AKNSVAIGHS SHVAANHGYS IAIGDRSKTD RENSVSIGHE SLNRQLTHLA 200
    AGTKDTDAVN VAQLKKEIEK TQENTNKRSA ELLANANAYA DNKSSSVLGI 250
    ANNYTDSKSA ETLENARKEA FAQSKDVLNM AKAHSNSVAR TTLETAEEHA 300
    NSVARTTLET AEEHANKKSA EALASANVYA DSKSSHTLKT ANSYTDVTVS 350
    NSTKKAIRES NQYTDHKFRQ LDNRLDKLDT RVDKGLASSA ALNSLFQPYG 400
    VGKVNFTAGV GGYRSSQALA IGSGYRVNEN VALKAGVAYA GSSDVMYNAS 450
    FNIEW 455
    Length:455
    Mass (Da):47,136
    Last modified:July 1, 1993 - v1
    Checksum:iAC12EF68C657DAC0
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti29 – 291D → N in strain: Serotype O:9.
    Natural varianti49 – 491L → Q in strain: Serotype O:9.
    Natural varianti65 – 651A → C in strain: Serotype O:9.
    Natural varianti228 – 2281R → K in strain: Serotype O:9.
    Natural varianti235 – 2362NA → KP in strain: Serotype O:9.
    Natural varianti435 – 4406AGVAYA → PVWLI in strain: Serotype O:9.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13882 Genomic DNA. Translation: CAA32086.1.
    AF056092 Genomic DNA. Translation: AAC33679.1.
    AF102990 Genomic DNA. Translation: AAD16868.1.
    PIRiS04912.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X13882 Genomic DNA. Translation: CAA32086.1 .
    AF056092 Genomic DNA. Translation: AAC33679.1 .
    AF102990 Genomic DNA. Translation: AAD16868.1 .
    PIRi S04912.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1P9H X-ray 1.55 A 26-241 [» ]
    3LT6 X-ray 1.80 A/B/C/D/E/F 333-396 [» ]
    3LT7 X-ray 1.50 A/B/C/D/E/F 333-396 [» ]
    ProteinModelPortali P31489.
    SMRi P31489. Positions 32-220.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P31489.

    Family and domain databases

    Gene3Di 2.150.10.10. 1 hit.
    InterProi IPR008640. Adhesin_Head_dom.
    IPR008635. Coiled_stalk_dom.
    IPR008126. OM_adhesion_Yersinia.
    IPR011049. Serralysin-like_metalloprot_C.
    IPR005594. YadA_C.
    [Graphical view ]
    Pfami PF03895. YadA_anchor. 1 hit.
    PF05658. YadA_head. 2 hits.
    PF05662. YadA_stalk. 1 hit.
    [Graphical view ]
    PRINTSi PR01756. OMADHESIN.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the yopA gene encoding the Yop1 virulence determinants of Yersinia spp."
      Skurnik M., Wolf-Watz H.
      Mol. Microbiol. 3:517-529(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 6471/76 / Serotype O:3.
      Plasmid: pYV6471/76
    2. "Hydrophobic domains affect the collagen-binding specificity and surface polymerization as well as the virulence potential of the YadA protein of Yersinia enterocolitica."
      Tamm A., Tarkkanen A., Korhonen T.K., Kuusela P., Toivanen P., Skurnik M.
      Mol. Microbiol. 10:995-1011(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 6471/76 / Serotype O:3.
      Plasmid: pYV6471/76
    3. "YadA and ORF291 of Yersinia enterocolitica O:9."
      Iriarte M., Kerbourch C., Lambermont I., Cornelis G.R.
      Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Serotype O:9.
      Plasmid: pYV
    4. "Detailed genetic map of the pYVe227 plasmid of Yersinia enterocolitica serotype O:9."
      Iriarte M., Lambermont I., Kerbourch C., Cornelis G.R.
      Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: W22703 / Serotype O:9 / Biotype 2.
      Plasmid: pYVe227
    5. "Binding to collagen by Yersinia enterocolitica and Yersinia pseudotuberculosis: evidence for yopA-mediated and chromosomally encoded mechanisms."
      Emoedy L., Heesemann J., Wolf-Watz H., Skurnik M., Kapperud G., O'Toole P., Wadstroem T.
      J. Bacteriol. 171:6674-6679(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: Various strains.
    6. "LcrF is the temperature-regulated activator of the yadA gene of Yersinia enterocolitica and Yersinia pseudotuberculosis."
      Skurnik M., Toivanen P.
      J. Bacteriol. 174:2047-2051(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: 6471/76 / Serotype O:3.
      Plasmid: pYV6471/76
    7. "Functional mapping of the Yersinia enterocolitica adhesin YadA. Identification of eight NSVAIG - S motifs in the amino-terminal half of the protein involved in collagen binding."
      Tahir Y.E., Kuusela P., Skurnik M.
      Mol. Microbiol. 37:192-206(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN COLLAGEN-BINDING, MUTAGENESIS OF 73-ILE--ILE-75; 101-VAL--ILE-103; 155-VAL--ILE-157; VAL-155; ALA-156; 171-ILE--ILE-173 AND 185-VAL--ILE-187.
      Strain: 6471/76 / Serotype O:3.
      Plasmid: pYV6471/76
    8. "Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA."
      Nummelin H., El Tahir Y., Ollikka P., Skurnik M., Goldman A.
      Acta Crystallogr. D 58:1042-1044(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRYSTALLIZATION.
      Strain: 6471/76 / Serotype O:3.
      Plasmid: pYV6471/76
    9. "The Yersinia adhesin YadA collagen-binding domain structure is a novel left-handed parallel beta-roll."
      Nummelin H., Merckel M.C., Leo J.C., Lankinen H., Skurnik M., Goldman A.
      EMBO J. 23:701-711(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF 26-241.
      Strain: 6471/76 / Serotype O:3.

    Entry informationi

    Entry nameiYADA1_YEREN
    AccessioniPrimary (citable) accession number: P31489
    Secondary accession number(s): O85267
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3