Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phospholipase A2, membrane associated

Gene

Pla2g2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:8425615). Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Independent of its catalytic activity, acts as a ligand for integrins. Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB. Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1. Induces cell proliferation in an integrin-dependent manner (By similarity).By similarity1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation1 Publication

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Calcium; via carbonyl oxygenBy similarity1
Metal bindingi50Calcium; via carbonyl oxygenBy similarity1
Metal bindingi52Calcium; via carbonyl oxygenBy similarity1
Active sitei68By similarity1
Metal bindingi69CalciumBy similarity1
Active sitei113By similarity1
Sitei122Important for integrin bindingBy similarity1

GO - Molecular functioni

GO - Biological processi

  • arachidonic acid secretion Source: InterPro
  • lipid catabolic process Source: UniProtKB-KW
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of epithelial cell proliferation Source: MGI
  • phospholipid metabolic process Source: InterPro
  • regulation of cell proliferation Source: MGI
  • regulation of epithelial cell proliferation Source: MGI
  • regulation of growth Source: UniProtKB-KW
  • somatic stem cell population maintenance Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Growth regulation, Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A2, membrane associated (EC:3.1.1.41 Publication)
Alternative name(s):
Enhancing factor
Short name:
EF
GIIC sPLA2
Group IIA phospholipase A2
Phosphatidylcholine 2-acylhydrolase 2A
Gene namesi
Name:Pla2g2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104642. Pla2g2a.

Subcellular locationi

  • Cell membrane Curated; Peripheral membrane protein By similarity
  • Secreted By similarity

GO - Cellular componenti

  • extracellular region Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5761.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000002275122 – 146Phospholipase A2, membrane associatedAdd BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 139By similarity
Disulfide bondi49 ↔ 65By similarity
Disulfide bondi64 ↔ 119By similarity
Disulfide bondi70 ↔ 146By similarity
Disulfide bondi71 ↔ 112By similarity
Disulfide bondi80 ↔ 105By similarity
Disulfide bondi98 ↔ 110By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP31482.

PTM databases

PhosphoSitePlusiP31482.

Expressioni

Tissue specificityi

Mainly in the Paneth cells adjacent to the stem population in the small intestines. Also expressed in regenerating liver and hyperplastic esophageal epithelium.

Interactioni

Protein-protein interaction databases

BioGridi202219. 4 interactors.

Chemistry databases

BindingDBiP31482.

Structurei

3D structure databases

ProteinModelPortaliP31482.
SMRiP31482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase A2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG008137.
InParanoidiP31482.
KOiK01047.
PhylomeDBiP31482.

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLLLLAAS IMAFGSIQVQ GNIAQFGEMI RLKTGKRAEL SYAFYGCHCG
60 70 80 90 100
LGGKGSPKDA TDRCCVTHDC CYKSLEKSGC GTKLLKYKYS HQGGQITCSA
110 120 130 140
NQNSCQKRLC QCDKAAAECF ARNKKTYSLK YQFYPNMFCK GKKPKC
Length:146
Mass (Da):16,145
Last modified:June 16, 2003 - v3
Checksum:iAB904F6B3B1BA5C7
GO

Sequence cautioni

The sequence AAB06315 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19V → D in CAA52325 (Ref. 1) Curated1
Sequence conflicti86K → T in CAA52325 (Ref. 1) Curated1

Polymorphismi

In strains 129/Sv, B10.RIII and C57BL/6, a polymorphism causes a frameshift and premature truncation of the protein, rendering it inactive. Strains BALB/c, C3H/He, DBA/1, DBA/2, MRL and NZB/B1N contain the normal protein while strain CD-1 is heterozygous for the mutation.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74266 mRNA. Translation: CAA52325.1.
U32358 Genomic DNA. Translation: AAC52252.1.
U28244 mRNA. Translation: AAB06315.1. Different initiation.
BC045156 mRNA. Translation: AAH45156.1.
PIRiI48342.
S29495.
RefSeqiNP_001076000.1. NM_001082531.1.
UniGeneiMm.4675.

Genome annotation databases

GeneIDi18780.
KEGGimmu:18780.
UCSCiuc008vlk.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74266 mRNA. Translation: CAA52325.1.
U32358 Genomic DNA. Translation: AAC52252.1.
U28244 mRNA. Translation: AAB06315.1. Different initiation.
BC045156 mRNA. Translation: AAH45156.1.
PIRiI48342.
S29495.
RefSeqiNP_001076000.1. NM_001082531.1.
UniGeneiMm.4675.

3D structure databases

ProteinModelPortaliP31482.
SMRiP31482.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202219. 4 interactors.

Chemistry databases

BindingDBiP31482.
ChEMBLiCHEMBL5761.

PTM databases

PhosphoSitePlusiP31482.

Proteomic databases

PRIDEiP31482.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18780.
KEGGimmu:18780.
UCSCiuc008vlk.1. mouse.

Organism-specific databases

CTDi5320.
MGIiMGI:104642. Pla2g2a.

Phylogenomic databases

HOVERGENiHBG008137.
InParanoidiP31482.
KOiK01047.
PhylomeDBiP31482.

Miscellaneous databases

PROiP31482.
SOURCEiSearch...

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA2GA_MOUSE
AccessioniPrimary (citable) accession number: P31482
Secondary accession number(s): Q60871
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: June 16, 2003
Last modified: November 30, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.