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Protein

Phospholipase A2, membrane associated

Gene

Pla2g2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:8425615). Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Independent of its catalytic activity, acts as a ligand for integrins. Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB. Binds to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and induces integrin conformational changes and enhanced ligand binding to site 1. Induces cell proliferation in an integrin-dependent manner (By similarity).By similarity1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation1 Publication

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Calcium; via carbonyl oxygenBy similarity
Metal bindingi50 – 501Calcium; via carbonyl oxygenBy similarity
Metal bindingi52 – 521Calcium; via carbonyl oxygenBy similarity
Active sitei68 – 681By similarity
Metal bindingi69 – 691CalciumBy similarity
Active sitei113 – 1131By similarity
Sitei122 – 1221Important for integrin bindingBy similarity

GO - Molecular functioni

GO - Biological processi

  • lipid catabolic process Source: UniProtKB-KW
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of epithelial cell proliferation Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of epithelial cell proliferation Source: MGI
  • regulation of growth Source: UniProtKB-KW
  • somatic stem cell population maintenance Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Growth regulation, Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A2, membrane associated (EC:3.1.1.41 Publication)
Alternative name(s):
Enhancing factor
Short name:
EF
GIIC sPLA2
Group IIA phospholipase A2
Phosphatidylcholine 2-acylhydrolase 2A
Gene namesi
Name:Pla2g2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104642. Pla2g2a.

Subcellular locationi

  • Cell membrane Curated; Peripheral membrane protein By similarity
  • Secreted By similarity

GO - Cellular componenti

  • membrane Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5761.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 146125Phospholipase A2, membrane associatedPRO_0000022751Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi47 ↔ 139By similarity
Disulfide bondi49 ↔ 65By similarity
Disulfide bondi64 ↔ 119By similarity
Disulfide bondi70 ↔ 146By similarity
Disulfide bondi71 ↔ 112By similarity
Disulfide bondi80 ↔ 105By similarity
Disulfide bondi98 ↔ 110By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP31482.

PTM databases

PhosphoSiteiP31482.

Expressioni

Tissue specificityi

Mainly in the Paneth cells adjacent to the stem population in the small intestines. Also expressed in regenerating liver and hyperplastic esophageal epithelium.

Interactioni

Protein-protein interaction databases

BioGridi202219. 4 interactions.

Chemistry

BindingDBiP31482.

Structurei

3D structure databases

ProteinModelPortaliP31482.
SMRiP31482. Positions 22-146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase A2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG008137.
InParanoidiP31482.
KOiK01047.
PhylomeDBiP31482.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLLLLAAS IMAFGSIQVQ GNIAQFGEMI RLKTGKRAEL SYAFYGCHCG
60 70 80 90 100
LGGKGSPKDA TDRCCVTHDC CYKSLEKSGC GTKLLKYKYS HQGGQITCSA
110 120 130 140
NQNSCQKRLC QCDKAAAECF ARNKKTYSLK YQFYPNMFCK GKKPKC
Length:146
Mass (Da):16,145
Last modified:June 16, 2003 - v3
Checksum:iAB904F6B3B1BA5C7
GO

Sequence cautioni

The sequence AAB06315.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191V → D in CAA52325 (Ref. 1) Curated
Sequence conflicti86 – 861K → T in CAA52325 (Ref. 1) Curated

Polymorphismi

In strains 129/Sv, B10.RIII and C57BL/6, a polymorphism causes a frameshift and premature truncation of the protein, rendering it inactive. Strains BALB/c, C3H/He, DBA/1, DBA/2, MRL and NZB/B1N contain the normal protein while strain CD-1 is heterozygous for the mutation.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74266 mRNA. Translation: CAA52325.1.
U32358 Genomic DNA. Translation: AAC52252.1.
U28244 mRNA. Translation: AAB06315.1. Different initiation.
BC045156 mRNA. Translation: AAH45156.1.
PIRiI48342.
S29495.
RefSeqiNP_001076000.1. NM_001082531.1.
UniGeneiMm.4675.

Genome annotation databases

GeneIDi18780.
KEGGimmu:18780.
UCSCiuc008vlk.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74266 mRNA. Translation: CAA52325.1.
U32358 Genomic DNA. Translation: AAC52252.1.
U28244 mRNA. Translation: AAB06315.1. Different initiation.
BC045156 mRNA. Translation: AAH45156.1.
PIRiI48342.
S29495.
RefSeqiNP_001076000.1. NM_001082531.1.
UniGeneiMm.4675.

3D structure databases

ProteinModelPortaliP31482.
SMRiP31482. Positions 22-146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202219. 4 interactions.

Chemistry

BindingDBiP31482.
ChEMBLiCHEMBL5761.

PTM databases

PhosphoSiteiP31482.

Proteomic databases

PRIDEiP31482.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18780.
KEGGimmu:18780.
UCSCiuc008vlk.1. mouse.

Organism-specific databases

CTDi5320.
MGIiMGI:104642. Pla2g2a.

Phylogenomic databases

HOVERGENiHBG008137.
InParanoidiP31482.
KOiK01047.
PhylomeDBiP31482.

Miscellaneous databases

PROiP31482.
SOURCEiSearch...

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mulherkar R.
    Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BALB/cJ.
  2. "A natural disruption of the secretory group II phospholipase A2 gene in inbred mouse strains."
    Kennedy B.P., Payette P., Mudgett J., Vadas P., Pruzanski W., Ywan M., Tang C., Rancourt D.E., Cromlish W.
    J. Biol. Chem. 270:22378-22385(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], POLYMORPHISM.
    Strain: BALB/cJ and CD-1.
    Tissue: Intestine.
  3. "The secretory phospholipase A2 gene is a candidate for the Mom1 locus, a major modifier of ApcMin-induced intestinal neoplasia."
    MacPhee M., Chepenik K.P., Liddell R.A., Nelson K.K., Siracusa L.D., Buchberg A.M.
    Cell 81:957-966(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H/HeJ.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.
  5. "Enhancing factor, a Paneth cell specific protein from mouse small intestines: predicted amino acid sequence from RT-PCR amplified cDNA and its expression."
    Mulherkar R., Rao R.S., Wagle A.S., Patki V., Deo M.G.
    Biochem. Biophys. Res. Commun. 195:1254-1263(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 22-146.
    Strain: BALB/cJ.
    Tissue: Small intestine.
  6. "Enhancing factor protein from mouse small intestines belongs to the phospholipase A2 family."
    Mulherkar R., Rao R., Rao L., Patki V., Chauhan V.S., Deo M.G.
    FEBS Lett. 317:263-266(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 22-41, FUNCTION, CATALYTIC ACTIVITY.
    Tissue: Small intestine.

Entry informationi

Entry nameiPA2GA_MOUSE
AccessioniPrimary (citable) accession number: P31482
Secondary accession number(s): Q60871
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: June 16, 2003
Last modified: July 6, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.