Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

6-phosphogluconate phosphatase

Gene

yieH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes strongly the dephosphorylation of 6-phosphogluconate (6P-Glu) and slightly the dephosphorylation of dihydroxyacetone phosphate (DHAP) and phosphoenolpyruvate (PEP). Also hydrolyzes both purines (GMP and IMP) and pyrimidines as secondary substrates.2 Publications

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Co2+1 Publication, Zn2+1 PublicationNote: Magnesium. Can also use other divalent metal cations as manganese, cobalt or zinc.1 Publication

Kineticsi

  1. KM=2.2 mM for 6P-Glu (with magnesium ions as cofactor and at pH 9)1 Publication
  2. KM=2.9 mM for PEP (with manganese ions as cofactor and at pH 9)1 Publication

    pH dependencei

    Optimum pH is between 6 and 7.5.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei10 – 101NucleophileBy similarity
    Metal bindingi10 – 101MagnesiumBy similarity
    Metal bindingi12 – 121MagnesiumBy similarity
    Metal bindingi167 – 1671MagnesiumBy similarity

    GO - Molecular functioni

    • magnesium ion binding Source: UniProtKB
    • manganese ion binding Source: UniProtKB
    • phosphatase activity Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11725-MONOMER.
    ECOL316407:JW3693-MONOMER.
    MetaCyc:EG11725-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phosphogluconate phosphatase (EC:3.1.3.-)
    Gene namesi
    Name:yieH
    Ordered Locus Names:b3715, JW3693
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11725. yieH.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2212216-phosphogluconate phosphatasePRO_0000108060Add
    BLAST

    Proteomic databases

    PaxDbiP31467.
    PRIDEiP31467.

    Interactioni

    Protein-protein interaction databases

    BioGridi4263102. 8 interactions.
    STRINGi511145.b3715.

    Structurei

    3D structure databases

    ProteinModelPortaliP31467.
    SMRiP31467. Positions 5-193.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni10 – 123Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4107UB3. Bacteria.
    COG0637. LUCA.
    HOGENOMiHOG000248341.
    InParanoidiP31467.
    OMAiRAYVAMF.
    PhylomeDBiP31467.

    Family and domain databases

    Gene3Di1.10.150.240. 1 hit.
    3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006439. HAD-SF_hydro_IA.
    IPR023198. PGP_dom2.
    [Graphical view]
    PfamiPF13419. HAD_2. 1 hit.
    [Graphical view]
    PRINTSiPR00413. HADHALOGNASE.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01509. HAD-SF-IA-v3. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P31467-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSRIEAVFFD CDGTLVDSEV ICSRAYVTMF QEFGITLDPE EVFKRFKGVK
    60 70 80 90 100
    LYEIIDIVSL EHGVTLAKTE AEHVYRAEVA RLFDSELEAI EGAGALLSAI
    110 120 130 140 150
    TAPMCVVSNG PNNKMQHSMG KLNMLHYFPD KLFSGYDIQR WKPDPALMFH
    160 170 180 190 200
    AAKAMNVNVE NCILVDDSVA GAQSGIDAGM EVFYFCADPH NKPIVHPKVT
    210 220
    TFTHLSQLPE LWKARGWDIT A
    Length:221
    Mass (Da):24,664
    Last modified:July 1, 1993 - v1
    Checksum:iC50CF0A788D7CE5F
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L10328 Genomic DNA. Translation: AAA62066.1.
    U00096 Genomic DNA. Translation: AAC76738.1.
    AP009048 Genomic DNA. Translation: BAE77573.1.
    PIRiD65174.
    RefSeqiNP_418171.1. NC_000913.3.
    WP_000086486.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76738; AAC76738; b3715.
    BAE77573; BAE77573; BAE77573.
    GeneIDi948232.
    KEGGiecj:JW3693.
    eco:b3715.
    PATRICi32122925. VBIEscCol129921_3838.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L10328 Genomic DNA. Translation: AAA62066.1.
    U00096 Genomic DNA. Translation: AAC76738.1.
    AP009048 Genomic DNA. Translation: BAE77573.1.
    PIRiD65174.
    RefSeqiNP_418171.1. NC_000913.3.
    WP_000086486.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP31467.
    SMRiP31467. Positions 5-193.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4263102. 8 interactions.
    STRINGi511145.b3715.

    Proteomic databases

    PaxDbiP31467.
    PRIDEiP31467.

    Protocols and materials databases

    DNASUi948232.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76738; AAC76738; b3715.
    BAE77573; BAE77573; BAE77573.
    GeneIDi948232.
    KEGGiecj:JW3693.
    eco:b3715.
    PATRICi32122925. VBIEscCol129921_3838.

    Organism-specific databases

    EchoBASEiEB1676.
    EcoGeneiEG11725. yieH.

    Phylogenomic databases

    eggNOGiENOG4107UB3. Bacteria.
    COG0637. LUCA.
    HOGENOMiHOG000248341.
    InParanoidiP31467.
    OMAiRAYVAMF.
    PhylomeDBiP31467.

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11725-MONOMER.
    ECOL316407:JW3693-MONOMER.
    MetaCyc:EG11725-MONOMER.

    Miscellaneous databases

    PROiP31467.

    Family and domain databases

    Gene3Di1.10.150.240. 1 hit.
    3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006439. HAD-SF_hydro_IA.
    IPR023198. PGP_dom2.
    [Graphical view]
    PfamiPF13419. HAD_2. 1 hit.
    [Graphical view]
    PRINTSiPR00413. HADHALOGNASE.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01509. HAD-SF-IA-v3. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiYIEH_ECOLI
    AccessioniPrimary (citable) accession number: P31467
    Secondary accession number(s): Q2M833
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: September 7, 2016
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.