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Protein

6-phosphogluconate phosphatase

Gene

yieH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes strongly the dephosphorylation of 6-phosphogluconate (6P-Glu) and slightly the dephosphorylation of dihydroxyacetone phosphate (DHAP) and phosphoenolpyruvate (PEP). Also hydrolyzes both purines (GMP and IMP) and pyrimidines as secondary substrates.2 Publications

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Co2+1 Publication, Zn2+1 PublicationNote: Magnesium. Can also use other divalent metal cations as manganese, cobalt or zinc.1 Publication

Kineticsi

  1. KM=2.2 mM for 6P-Glu (with magnesium ions as cofactor and at pH 9)1 Publication
  2. KM=2.9 mM for PEP (with manganese ions as cofactor and at pH 9)1 Publication

    pH dependencei

    Optimum pH is between 6 and 7.5.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei10NucleophileBy similarity1
    Metal bindingi10MagnesiumBy similarity1
    Metal bindingi12MagnesiumBy similarity1
    Metal bindingi167MagnesiumBy similarity1

    GO - Molecular functioni

    • 2-deoxyglucose-6-phosphatase activity Source: GO_Central
    • glucose-6-phosphatase activity Source: GO_Central
    • magnesium ion binding Source: UniProtKB
    • manganese ion binding Source: UniProtKB
    • phosphatase activity Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase
    LigandMagnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11725-MONOMER
    MetaCyc:EG11725-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phosphogluconate phosphatase (EC:3.1.3.-)
    Gene namesi
    Name:yieH
    Ordered Locus Names:b3715, JW3693
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11725 yieH

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001080601 – 2216-phosphogluconate phosphataseAdd BLAST221

    Proteomic databases

    PaxDbiP31467
    PRIDEiP31467

    Interactioni

    Protein-protein interaction databases

    BioGridi4263102, 8 interactors
    STRINGi316385.ECDH10B_3902

    Structurei

    3D structure databases

    ProteinModelPortaliP31467
    SMRiP31467
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni10 – 12Substrate bindingBy similarity3

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4107UB3 Bacteria
    COG0637 LUCA
    HOGENOMiHOG000248341
    InParanoidiP31467
    OMAiEFVGQNF
    PhylomeDBiP31467

    Family and domain databases

    Gene3Di1.10.150.240, 1 hit
    3.40.50.1000, 2 hits
    InterProiView protein in InterPro
    IPR036412 HAD-like_sf
    IPR006439 HAD-SF_hydro_IA
    IPR023214 HAD_sf
    IPR023198 PGP-like_dom2
    PRINTSiPR00413 HADHALOGNASE
    SUPFAMiSSF56784 SSF56784, 1 hit
    TIGRFAMsiTIGR01509 HAD-SF-IA-v3, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P31467-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSRIEAVFFD CDGTLVDSEV ICSRAYVTMF QEFGITLDPE EVFKRFKGVK
    60 70 80 90 100
    LYEIIDIVSL EHGVTLAKTE AEHVYRAEVA RLFDSELEAI EGAGALLSAI
    110 120 130 140 150
    TAPMCVVSNG PNNKMQHSMG KLNMLHYFPD KLFSGYDIQR WKPDPALMFH
    160 170 180 190 200
    AAKAMNVNVE NCILVDDSVA GAQSGIDAGM EVFYFCADPH NKPIVHPKVT
    210 220
    TFTHLSQLPE LWKARGWDIT A
    Length:221
    Mass (Da):24,664
    Last modified:July 1, 1993 - v1
    Checksum:iC50CF0A788D7CE5F
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L10328 Genomic DNA Translation: AAA62066.1
    U00096 Genomic DNA Translation: AAC76738.1
    AP009048 Genomic DNA Translation: BAE77573.1
    PIRiD65174
    RefSeqiNP_418171.1, NC_000913.3
    WP_000086486.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC76738; AAC76738; b3715
    BAE77573; BAE77573; BAE77573
    GeneIDi948232
    KEGGiecj:JW3693
    eco:b3715
    PATRICifig|1411691.4.peg.2986

    Similar proteinsi

    Entry informationi

    Entry nameiYIEH_ECOLI
    AccessioniPrimary (citable) accession number: P31467
    Secondary accession number(s): Q2M833
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: March 28, 2018
    This is version 124 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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