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Protein

Adenine permease AdeP

Gene

adeP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity transporter for adenine.3 Publications

Enzyme regulationi

Internal adenine may inhibit transport.1 Publication

Kineticsi

  1. KM=1.0 µM for adenine1 Publication
  1. Vmax=1.7 nmol/min/mg enzyme1 Publication

GO - Molecular functioni

  • adenine transmembrane transporter activity Source: EcoCyc
  • solute:proton symporter activity Source: EcoCyc

GO - Biological processi

  • adenine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11724-MONOMER.
ECOL316407:JW3692-MONOMER.
MetaCyc:EG11724-MONOMER.

Protein family/group databases

TCDBi2.A.40.7.6. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine permease AdeP
Gene namesi
Name:adeP
Synonyms:purP, yieG
Ordered Locus Names:b3714, JW3692
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11724. adeP.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 28CytoplasmicSequence analysisAdd BLAST28
Transmembranei29 – 52HelicalSequence analysisAdd BLAST24
Topological domaini53 – 62PeriplasmicSequence analysis10
Transmembranei63 – 81HelicalSequence analysisAdd BLAST19
Topological domaini82 – 83CytoplasmicSequence analysis2
Transmembranei84 – 100Discontinuously helicalSequence analysisAdd BLAST17
Topological domaini101 – 112PeriplasmicSequence analysisAdd BLAST12
Transmembranei113 – 132HelicalSequence analysisAdd BLAST20
Topological domaini133 – 144CytoplasmicSequence analysisAdd BLAST12
Transmembranei145 – 165HelicalSequence analysisAdd BLAST21
Topological domaini166 – 181PeriplasmicSequence analysisAdd BLAST16
Transmembranei182 – 199HelicalSequence analysisAdd BLAST18
Topological domaini200 – 203CytoplasmicSequence analysis4
Transmembranei204 – 222HelicalSequence analysisAdd BLAST19
Topological domaini223 – 250PeriplasmicSequence analysisAdd BLAST28
Transmembranei251 – 279HelicalSequence analysisAdd BLAST29
Topological domaini280 – 292CytoplasmicSequence analysisAdd BLAST13
Transmembranei293 – 308HelicalSequence analysisAdd BLAST16
Topological domaini309 – 310PeriplasmicSequence analysis2
Transmembranei311 – 326Discontinuously helicalSequence analysisAdd BLAST16
Topological domaini327 – 330CytoplasmicSequence analysis4
Transmembranei331 – 345HelicalSequence analysisAdd BLAST15
Topological domaini346 – 356PeriplasmicSequence analysisAdd BLAST11
Transmembranei357 – 376HelicalSequence analysisAdd BLAST20
Topological domaini377 – 381CytoplasmicSequence analysis5
Intramembranei382 – 417Discontinuously helicalSequence analysisAdd BLAST36
Topological domaini418 – 445CytoplasmicSequence analysisAdd BLAST28

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38T → A: Almost no change in activity. 1 Publication1
Mutagenesisi38T → H: Lack of activity. 1 Publication1
Mutagenesisi91A → G or S: Almost no change in activity. 1 Publication1
Mutagenesisi267D → A or N: Lack of activity. 1 Publication1
Mutagenesisi267D → E: Strong decrease in activity. 1 Publication1
Mutagenesisi271T → A or S: Almost no change in activity. 1 Publication1
Mutagenesisi271T → D: Lack of activity. 1 Publication1
Mutagenesisi271T → N: Strong decrease in activity. 1 Publication1
Mutagenesisi298D → E: Almost no change in activity. 1 Publication1
Mutagenesisi298D → N: Lack of activity. 1 Publication1
Mutagenesisi317I → A: Almost no change in activity. 1 Publication1
Mutagenesisi317I → E: Strong decrease in activity. 1 Publication1
Mutagenesisi318E → D: Strong decrease in activity. 1 Publication1
Mutagenesisi318E → Q: Lack of activity. 1 Publication1
Mutagenesisi319S → A or N: Almost no change in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001696381 – 445Adenine permease AdePAdd BLAST445

Proteomic databases

PaxDbiP31466.
PRIDEiP31466.

Interactioni

Protein-protein interaction databases

BioGridi4262172. 244 interactors.
STRINGi511145.b3714.

Structurei

3D structure databases

ProteinModelPortaliP31466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C7N. Bacteria.
COG2252. LUCA.
HOGENOMiHOG000244362.
InParanoidiP31466.
KOiK06901.
OMAiMVDFFDT.
PhylomeDBiP31466.

Family and domain databases

InterProiIPR029940. AzgA.
IPR026033. Pur_Permease_PbuG-like.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PTHR11119:SF9. PTHR11119:SF9. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF005353. PbuG. 1 hit.

Sequencei

Sequence statusi: Complete.

P31466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHQHTTQTS GQGMLERVFK LREHGTTART EVIAGFTTFL TMVYIVFVNP
60 70 80 90 100
QILGVAGMDT SAVFVTTCLI AAFGSIMMGL FANLPVALAP AMGLNAFFAF
110 120 130 140 150
VVVQAMGLPW QVGMGAIFWG AIGLLLLTIF RVRYWMIANI PVSLRVGITS
160 170 180 190 200
GIGLFIGMMG LKNAGVIVAN PETLVSIGNL TSHSVLLGIL GFFIIAILAS
210 220 230 240 250
RNIHAAVLVS IVVTTLLGWM LGDVHYNGIV SAPPSVMTVV GHVDLAGSFN
260 270 280 290 300
LGLAGVIFSF MLVNLFDSSG TLIGVTDKAG LADEKGKFPR MKQALYVDSI
310 320 330 340 350
SSVTGSFIGT SSVTAYIESS SGVSVGGRTG LTAVVVGLLF LLVIFLSPLA
360 370 380 390 400
GMVPGYAAAG ALIYVGVLMT SSLARVNWQD LTESVPAFIT AVMMPFSFSI
410 420 430 440
TEGIALGFIS YCVMKIGTGR LRDLSPCVII VALLFILKIV FIDAH
Length:445
Mass (Da):46,866
Last modified:November 1, 1997 - v2
Checksum:i899FC4A38FD62E2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62065.1.
U00096 Genomic DNA. Translation: AAC76737.1.
AP009048 Genomic DNA. Translation: BAE77574.1.
PIRiC65174.
RefSeqiNP_418170.1. NC_000913.3.
WP_000019348.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76737; AAC76737; b3714.
BAE77574; BAE77574; BAE77574.
GeneIDi948224.
KEGGiecj:JW3692.
eco:b3714.
PATRICi32122923. VBIEscCol129921_3837.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62065.1.
U00096 Genomic DNA. Translation: AAC76737.1.
AP009048 Genomic DNA. Translation: BAE77574.1.
PIRiC65174.
RefSeqiNP_418170.1. NC_000913.3.
WP_000019348.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP31466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262172. 244 interactors.
STRINGi511145.b3714.

Protein family/group databases

TCDBi2.A.40.7.6. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Proteomic databases

PaxDbiP31466.
PRIDEiP31466.

Protocols and materials databases

DNASUi948224.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76737; AAC76737; b3714.
BAE77574; BAE77574; BAE77574.
GeneIDi948224.
KEGGiecj:JW3692.
eco:b3714.
PATRICi32122923. VBIEscCol129921_3837.

Organism-specific databases

EchoBASEiEB1675.
EcoGeneiEG11724. adeP.

Phylogenomic databases

eggNOGiENOG4105C7N. Bacteria.
COG2252. LUCA.
HOGENOMiHOG000244362.
InParanoidiP31466.
KOiK06901.
OMAiMVDFFDT.
PhylomeDBiP31466.

Enzyme and pathway databases

BioCyciEcoCyc:EG11724-MONOMER.
ECOL316407:JW3692-MONOMER.
MetaCyc:EG11724-MONOMER.

Miscellaneous databases

PROiP31466.

Family and domain databases

InterProiIPR029940. AzgA.
IPR026033. Pur_Permease_PbuG-like.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PTHR11119:SF9. PTHR11119:SF9. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF005353. PbuG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADEP_ECOLI
AccessioniPrimary (citable) accession number: P31466
Secondary accession number(s): P76741, Q2M832
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.