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Protein

Adenine permease AdeP

Gene

adeP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity transporter for adenine.3 Publications

Enzyme regulationi

Internal adenine may inhibit transport.1 Publication

Kineticsi

  1. KM=1.0 µM for adenine1 Publication
  1. Vmax=1.7 nmol/min/mg enzyme1 Publication

GO - Molecular functioni

  • adenine transmembrane transporter activity Source: EcoCyc
  • solute:proton symporter activity Source: EcoCyc

GO - Biological processi

  • adenine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11724-MONOMER.
ECOL316407:JW3692-MONOMER.
MetaCyc:EG11724-MONOMER.

Protein family/group databases

TCDBi2.A.40.7.6. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine permease AdeP
Gene namesi
Name:adeP
Synonyms:purP, yieG
Ordered Locus Names:b3714, JW3692
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11724. adeP.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828CytoplasmicSequence analysisAdd
BLAST
Transmembranei29 – 5224HelicalSequence analysisAdd
BLAST
Topological domaini53 – 6210PeriplasmicSequence analysis
Transmembranei63 – 8119HelicalSequence analysisAdd
BLAST
Topological domaini82 – 832CytoplasmicSequence analysis
Transmembranei84 – 10017Discontinuously helicalSequence analysisAdd
BLAST
Topological domaini101 – 11212PeriplasmicSequence analysisAdd
BLAST
Transmembranei113 – 13220HelicalSequence analysisAdd
BLAST
Topological domaini133 – 14412CytoplasmicSequence analysisAdd
BLAST
Transmembranei145 – 16521HelicalSequence analysisAdd
BLAST
Topological domaini166 – 18116PeriplasmicSequence analysisAdd
BLAST
Transmembranei182 – 19918HelicalSequence analysisAdd
BLAST
Topological domaini200 – 2034CytoplasmicSequence analysis
Transmembranei204 – 22219HelicalSequence analysisAdd
BLAST
Topological domaini223 – 25028PeriplasmicSequence analysisAdd
BLAST
Transmembranei251 – 27929HelicalSequence analysisAdd
BLAST
Topological domaini280 – 29213CytoplasmicSequence analysisAdd
BLAST
Transmembranei293 – 30816HelicalSequence analysisAdd
BLAST
Topological domaini309 – 3102PeriplasmicSequence analysis
Transmembranei311 – 32616Discontinuously helicalSequence analysisAdd
BLAST
Topological domaini327 – 3304CytoplasmicSequence analysis
Transmembranei331 – 34515HelicalSequence analysisAdd
BLAST
Topological domaini346 – 35611PeriplasmicSequence analysisAdd
BLAST
Transmembranei357 – 37620HelicalSequence analysisAdd
BLAST
Topological domaini377 – 3815CytoplasmicSequence analysis
Intramembranei382 – 41736Discontinuously helicalSequence analysisAdd
BLAST
Topological domaini418 – 44528CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi38 – 381T → A: Almost no change in activity. 1 Publication
Mutagenesisi38 – 381T → H: Lack of activity. 1 Publication
Mutagenesisi91 – 911A → G or S: Almost no change in activity. 1 Publication
Mutagenesisi267 – 2671D → A or N: Lack of activity. 1 Publication
Mutagenesisi267 – 2671D → E: Strong decrease in activity. 1 Publication
Mutagenesisi271 – 2711T → A or S: Almost no change in activity. 1 Publication
Mutagenesisi271 – 2711T → D: Lack of activity. 1 Publication
Mutagenesisi271 – 2711T → N: Strong decrease in activity. 1 Publication
Mutagenesisi298 – 2981D → E: Almost no change in activity. 1 Publication
Mutagenesisi298 – 2981D → N: Lack of activity. 1 Publication
Mutagenesisi317 – 3171I → A: Almost no change in activity. 1 Publication
Mutagenesisi317 – 3171I → E: Strong decrease in activity. 1 Publication
Mutagenesisi318 – 3181E → D: Strong decrease in activity. 1 Publication
Mutagenesisi318 – 3181E → Q: Lack of activity. 1 Publication
Mutagenesisi319 – 3191S → A or N: Almost no change in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445Adenine permease AdePPRO_0000169638Add
BLAST

Proteomic databases

PaxDbiP31466.
PRIDEiP31466.

Interactioni

Protein-protein interaction databases

BioGridi4262172. 244 interactions.
STRINGi511145.b3714.

Structurei

3D structure databases

ProteinModelPortaliP31466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C7N. Bacteria.
COG2252. LUCA.
HOGENOMiHOG000244362.
InParanoidiP31466.
KOiK06901.
OMAiMVDFFDT.
OrthoDBiEOG6DNT97.
PhylomeDBiP31466.

Family and domain databases

InterProiIPR029940. AzgA.
IPR026033. Pur_Permease_PbuG-like.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PTHR11119:SF9. PTHR11119:SF9. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF005353. PbuG. 1 hit.

Sequencei

Sequence statusi: Complete.

P31466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHQHTTQTS GQGMLERVFK LREHGTTART EVIAGFTTFL TMVYIVFVNP
60 70 80 90 100
QILGVAGMDT SAVFVTTCLI AAFGSIMMGL FANLPVALAP AMGLNAFFAF
110 120 130 140 150
VVVQAMGLPW QVGMGAIFWG AIGLLLLTIF RVRYWMIANI PVSLRVGITS
160 170 180 190 200
GIGLFIGMMG LKNAGVIVAN PETLVSIGNL TSHSVLLGIL GFFIIAILAS
210 220 230 240 250
RNIHAAVLVS IVVTTLLGWM LGDVHYNGIV SAPPSVMTVV GHVDLAGSFN
260 270 280 290 300
LGLAGVIFSF MLVNLFDSSG TLIGVTDKAG LADEKGKFPR MKQALYVDSI
310 320 330 340 350
SSVTGSFIGT SSVTAYIESS SGVSVGGRTG LTAVVVGLLF LLVIFLSPLA
360 370 380 390 400
GMVPGYAAAG ALIYVGVLMT SSLARVNWQD LTESVPAFIT AVMMPFSFSI
410 420 430 440
TEGIALGFIS YCVMKIGTGR LRDLSPCVII VALLFILKIV FIDAH
Length:445
Mass (Da):46,866
Last modified:November 1, 1997 - v2
Checksum:i899FC4A38FD62E2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62065.1.
U00096 Genomic DNA. Translation: AAC76737.1.
AP009048 Genomic DNA. Translation: BAE77574.1.
PIRiC65174.
RefSeqiNP_418170.1. NC_000913.3.
WP_000019348.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76737; AAC76737; b3714.
BAE77574; BAE77574; BAE77574.
GeneIDi948224.
KEGGiecj:JW3692.
eco:b3714.
PATRICi32122923. VBIEscCol129921_3837.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62065.1.
U00096 Genomic DNA. Translation: AAC76737.1.
AP009048 Genomic DNA. Translation: BAE77574.1.
PIRiC65174.
RefSeqiNP_418170.1. NC_000913.3.
WP_000019348.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP31466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262172. 244 interactions.
STRINGi511145.b3714.

Protein family/group databases

TCDBi2.A.40.7.6. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Proteomic databases

PaxDbiP31466.
PRIDEiP31466.

Protocols and materials databases

DNASUi948224.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76737; AAC76737; b3714.
BAE77574; BAE77574; BAE77574.
GeneIDi948224.
KEGGiecj:JW3692.
eco:b3714.
PATRICi32122923. VBIEscCol129921_3837.

Organism-specific databases

EchoBASEiEB1675.
EcoGeneiEG11724. adeP.

Phylogenomic databases

eggNOGiENOG4105C7N. Bacteria.
COG2252. LUCA.
HOGENOMiHOG000244362.
InParanoidiP31466.
KOiK06901.
OMAiMVDFFDT.
OrthoDBiEOG6DNT97.
PhylomeDBiP31466.

Enzyme and pathway databases

BioCyciEcoCyc:EG11724-MONOMER.
ECOL316407:JW3692-MONOMER.
MetaCyc:EG11724-MONOMER.

Miscellaneous databases

PROiP31466.

Family and domain databases

InterProiIPR029940. AzgA.
IPR026033. Pur_Permease_PbuG-like.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PTHR11119:SF9. PTHR11119:SF9. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF005353. PbuG. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Transport of nucleic acid bases into Escherichia coli."
    Burton K.
    J. Gen. Microbiol. 129:3505-3513(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Adenine transport in Escherichia coli."
    Burton K.
    Proc. R. Soc. B 255:153-157(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Functional identification of the hypoxanthine/guanine transporters YjcD and YgfQ and the adenine transporters PurP and YicO of Escherichia coli K-12."
    Papakostas K., Botou M., Frillingos S.
    J. Biol. Chem. 288:36827-36840(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TOPOLOGY, GENE NAME, MUTAGENESIS OF THR-38; ALA-91; ASP-267; THR-271; ASP-298; ILE-317; GLU-318 AND SER-319.
    Strain: K12.

Entry informationi

Entry nameiADEP_ECOLI
AccessioniPrimary (citable) accession number: P31466
Secondary accession number(s): P76741, Q2M832
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: July 6, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.