##gff-version 3
P31460	UniProtKB	Chain	1	229	.	.	.	ID=PRO_0000050621;Note=Galactonate operon transcriptional repressor	
P31460	UniProtKB	Domain	1	71	.	.	.	Note=HTH gntR-type;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00307	
P31460	UniProtKB	DNA binding	31	50	.	.	.	Note=H-T-H motif;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00307	
P31460	UniProtKB	Binding site	146	146	.	.	.	Ontology_term=ECO:0000269,ECO:0007744;evidence=ECO:0000269|PubMed:33224125,ECO:0007744|PDB:7C7E;Dbxref=PMID:33224125	
P31460	UniProtKB	Binding site	150	150	.	.	.	Ontology_term=ECO:0000269,ECO:0007744;evidence=ECO:0000269|PubMed:33224125,ECO:0007744|PDB:7C7E;Dbxref=PMID:33224125	
P31460	UniProtKB	Binding site	195	195	.	.	.	Ontology_term=ECO:0000269,ECO:0007744;evidence=ECO:0000269|PubMed:33224125,ECO:0007744|PDB:7C7E;Dbxref=PMID:33224125	
P31460	UniProtKB	Mutagenesis	7	7	.	.	.	Note=Loss of DNA-binding ability and repressor activity. D->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30455279;Dbxref=PMID:30455279	
P31460	UniProtKB	Mutagenesis	34	34	.	.	.	Note=Loss of DNA-binding ability and repressor activity. L->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30455279;Dbxref=PMID:30455279	
P31460	UniProtKB	Mutagenesis	40	40	.	.	.	Note=Loss of DNA-binding ability and repressor activity. T->I;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30455279;Dbxref=PMID:30455279	
P31460	UniProtKB	Mutagenesis	42	42	.	.	.	Note=Loss of DNA-binding ability and repressor activity. R->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30455279;Dbxref=PMID:30455279	
P31460	UniProtKB	Mutagenesis	46	46	.	.	.	Note=Loss of DNA-binding ability and repressor activity. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30455279;Dbxref=PMID:30455279	
P31460	UniProtKB	Mutagenesis	51	51	.	.	.	Note=Loss of DNA-binding ability and repressor activity. S->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:30455279;Dbxref=PMID:30455279	
P31460	UniProtKB	Mutagenesis	102	102	.	.	.	Note=Shows a decreased binding to both DNA and D-galactonate. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33224125;Dbxref=PMID:33224125	
P31460	UniProtKB	Mutagenesis	102	102	.	.	.	Note=Completely non-responsive to D-galactonate. Does not affect binding affinity for the dgo operator. R->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33068046;Dbxref=PMID:33068046	
P31460	UniProtKB	Mutagenesis	106	106	.	.	.	Note=Completely non-responsive to D-galactonate. Does not affect binding affinity for the dgo operator. E->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33068046;Dbxref=PMID:33068046	
P31460	UniProtKB	Mutagenesis	146	146	.	.	.	Note=Shows increased DNA binding but can be slightly reverted in the presence of D-galactonate. Strong decrease in binding to D-galactonate. D->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33224125;Dbxref=PMID:33224125	
P31460	UniProtKB	Mutagenesis	146	146	.	.	.	Note=Completely non-responsive to D-galactonate. Shows a slight loss of repression ability. D->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33068046;Dbxref=PMID:33068046	
P31460	UniProtKB	Mutagenesis	150	150	.	.	.	Note=Shows a decreased binding to both DNA and D-galactonate. H->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33224125;Dbxref=PMID:33224125	
P31460	UniProtKB	Mutagenesis	150	150	.	.	.	Note=Completely non-responsive to D-galactonate. H->Y;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33068046;Dbxref=PMID:33068046	
P31460	UniProtKB	Mutagenesis	173	173	.	.	.	Note=Shows a decreased binding to both DNA and D-galactonate. Q->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33224125;Dbxref=PMID:33224125	
P31460	UniProtKB	Mutagenesis	179	179	.	.	.	Note=Shows no significant effect on the binding to DNA and D-galactonate. R->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33224125;Dbxref=PMID:33224125	
P31460	UniProtKB	Mutagenesis	181	181	.	.	.	Note=Shows no significant effect on the binding to DNA and D-galactonate. W->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33224125;Dbxref=PMID:33224125	
P31460	UniProtKB	Mutagenesis	184	184	.	.	.	Note=Shows a decreased binding to both DNA and D-galactonate. D->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33224125;Dbxref=PMID:33224125	
P31460	UniProtKB	Mutagenesis	191	191	.	.	.	Note=Shows a decreased binding to both DNA and D-galactonate. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33224125;Dbxref=PMID:33224125	
P31460	UniProtKB	Mutagenesis	191	191	.	.	.	Note=Completely non-responsive to D-galactonate. Does not affect binding affinity for the dgo operator. T->M;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33068046;Dbxref=PMID:33068046	
P31460	UniProtKB	Mutagenesis	192	192	.	.	.	Note=Completely non-responsive to D-galactonate. Does not affect binding affinity for the dgo operator. L->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33068046;Dbxref=PMID:33068046	
P31460	UniProtKB	Mutagenesis	195	195	.	.	.	Note=Shows a decreased binding to both DNA and D-galactonate. H->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33224125;Dbxref=PMID:33224125	
P31460	UniProtKB	Mutagenesis	195	195	.	.	.	Note=Completely non-responsive to D-galactonate. H->Y;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33068046;Dbxref=PMID:33068046	
P31460	UniProtKB	Mutagenesis	221	221	.	.	.	Note=Shows increased DNA binding but can be slightly reverted in the presence of D-galactonate. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33224125;Dbxref=PMID:33224125	
P31460	UniProtKB	Mutagenesis	221	221	.	.	.	Note=Decreases sensitivity to D-galactonate. Does not affect binding affinity for the dgo operator. S->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33068046;Dbxref=PMID:33068046	
P31460	UniProtKB	Mutagenesis	224	224	.	.	.	Note=Shows a decreased binding to both DNA and D-galactonate. R->A%2CE;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:33224125;Dbxref=PMID:33224125	
P31460	UniProtKB	Sequence conflict	111	112	.	.	.	Note=RW->VA;Ontology_term=ECO:0000305;evidence=ECO:0000305