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Protein

Multidrug resistance protein D

Gene

emrD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Multidrug resistance pump that participates in a low energy shock adaptive response.

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:EMRD-MONOMER.
ECOL316407:JW5634-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.9. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein D
Gene namesi
Name:emrD
Synonyms:yicQ
Ordered Locus Names:b3673, JW5634
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11693. emrD.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 46PeriplasmicSequence analysisAdd BLAST17
Transmembranei47 – 67HelicalSequence analysisAdd BLAST21
Topological domaini68 – 73CytoplasmicSequence analysis6
Transmembranei74 – 94HelicalSequence analysisAdd BLAST21
Topological domaini95PeriplasmicSequence analysis1
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 134CytoplasmicSequence analysisAdd BLAST18
Transmembranei135 – 155HelicalSequence analysisAdd BLAST21
Topological domaini156 – 162PeriplasmicSequence analysis7
Transmembranei163 – 183HelicalSequence analysisAdd BLAST21
Topological domaini184 – 212CytoplasmicSequence analysisAdd BLAST29
Transmembranei213 – 233HelicalSequence analysisAdd BLAST21
Topological domaini234 – 242PeriplasmicSequence analysis9
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 276CytoplasmicSequence analysisAdd BLAST13
Transmembranei277 – 297HelicalSequence analysisAdd BLAST21
Topological domaini298PeriplasmicSequence analysis1
Transmembranei299 – 319HelicalSequence analysisAdd BLAST21
Topological domaini320 – 329CytoplasmicSequence analysis10
Transmembranei330 – 350HelicalSequence analysisAdd BLAST21
Topological domaini351 – 364PeriplasmicSequence analysisAdd BLAST14
Transmembranei365 – 385HelicalSequence analysisAdd BLAST21
Topological domaini386 – 394CytoplasmicSequence analysis9

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001733261 – 394Multidrug resistance protein DAdd BLAST394

Proteomic databases

PaxDbiP31442.
PRIDEiP31442.

Interactioni

Protein-protein interaction databases

BioGridi4261242. 128 interactors.
STRINGi511145.b3673.

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 35Combined sources26
Beta strandi38 – 40Combined sources3
Helixi43 – 60Combined sources18
Helixi63 – 67Combined sources5
Helixi72 – 76Combined sources5
Helixi77 – 126Combined sources50
Helixi133 – 154Combined sources22
Helixi158 – 176Combined sources19
Turni188 – 190Combined sources3
Turni196 – 198Combined sources3
Helixi202 – 229Combined sources28
Helixi235 – 250Combined sources16
Helixi252 – 264Combined sources13
Turni265 – 268Combined sources4
Helixi269 – 283Combined sources15
Beta strandi285 – 289Combined sources5
Helixi290 – 315Combined sources26
Helixi321 – 325Combined sources5
Helixi327 – 348Combined sources22
Helixi356 – 377Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GFPX-ray3.50A/B9-383[»]
ProteinModelPortaliP31442.
SMRiP31442.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31442.

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105E5M. Bacteria.
ENOG410XQPV. LUCA.
HOGENOMiHOG000276020.
InParanoidiP31442.
KOiK08154.
OMAiGLNSMTV.
PhylomeDBiP31442.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRQRNVNLL LMLVLLVAVG QMAQTIYIPA IADMARDLNV REGAVQSVMG
60 70 80 90 100
AYLLTYGVSQ LFYGPISDRV GRRPVILVGM SIFMLATLVA VTTSSLTVLI
110 120 130 140 150
AASAMQGMGT GVGGVMARTL PRDLYERTQL RHANSLLNMG ILVSPLLAPL
160 170 180 190 200
IGGLLDTMWN WRACYLFLLV LCAGVTFSMA RWMPETRPVD APRTRLLTSY
210 220 230 240 250
KTLFGNSGFN CYLLMLIGGL AGIAAFEACS GVLMGAVLGL SSMTVSILFI
260 270 280 290 300
LPIPAAFFGA WFAGRPNKRF STLMWQSVIC CLLAGLLMWI PDWFGVMNVW
310 320 330 340 350
TLLVPAALFF FGAGMLFPLA TSGAMEPFPF LAGTAGALVG GLQNIGSGVL
360 370 380 390
ASLSAMLPQT GQGSLGLLMT LMGLLIVLCW LPLATRMSHQ GQPV
Length:394
Mass (Da):42,216
Last modified:April 27, 2001 - v2
Checksum:i342339DC7265A30B
GO

Sequence cautioni

The sequence AAA62025 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62025.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76696.2.
AP009048 Genomic DNA. Translation: BAE77620.1.
PIRiB65169.
RefSeqiNP_418129.2. NC_000913.3.
WP_000828746.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76696; AAC76696; b3673.
BAE77620; BAE77620; BAE77620.
GeneIDi948180.
KEGGiecj:JW5634.
eco:b3673.
PATRICi32122837. VBIEscCol129921_3794.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62025.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76696.2.
AP009048 Genomic DNA. Translation: BAE77620.1.
PIRiB65169.
RefSeqiNP_418129.2. NC_000913.3.
WP_000828746.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GFPX-ray3.50A/B9-383[»]
ProteinModelPortaliP31442.
SMRiP31442.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261242. 128 interactors.
STRINGi511145.b3673.

Protein family/group databases

TCDBi2.A.1.2.9. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP31442.
PRIDEiP31442.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76696; AAC76696; b3673.
BAE77620; BAE77620; BAE77620.
GeneIDi948180.
KEGGiecj:JW5634.
eco:b3673.
PATRICi32122837. VBIEscCol129921_3794.

Organism-specific databases

EchoBASEiEB1644.
EcoGeneiEG11693. emrD.

Phylogenomic databases

eggNOGiENOG4105E5M. Bacteria.
ENOG410XQPV. LUCA.
HOGENOMiHOG000276020.
InParanoidiP31442.
KOiK08154.
OMAiGLNSMTV.
PhylomeDBiP31442.

Enzyme and pathway databases

BioCyciEcoCyc:EMRD-MONOMER.
ECOL316407:JW5634-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP31442.
PROiP31442.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEMRD_ECOLI
AccessioniPrimary (citable) accession number: P31442
Secondary accession number(s): Q2M7Y6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: April 27, 2001
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.