Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Multidrug resistance protein D

Gene

emrD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Multidrug resistance pump that participates in a low energy shock adaptive response.

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:EMRD-MONOMER.
ECOL316407:JW5634-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.9. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein D
Gene namesi
Name:emrD
Synonyms:yicQ
Ordered Locus Names:b3673, JW5634
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11693. emrD.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence analysis
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Topological domaini30 – 4617PeriplasmicSequence analysisAdd
BLAST
Transmembranei47 – 6721HelicalSequence analysisAdd
BLAST
Topological domaini68 – 736CytoplasmicSequence analysis
Transmembranei74 – 9421HelicalSequence analysisAdd
BLAST
Topological domaini95 – 951PeriplasmicSequence analysis
Transmembranei96 – 11621HelicalSequence analysisAdd
BLAST
Topological domaini117 – 13418CytoplasmicSequence analysisAdd
BLAST
Transmembranei135 – 15521HelicalSequence analysisAdd
BLAST
Topological domaini156 – 1627PeriplasmicSequence analysis
Transmembranei163 – 18321HelicalSequence analysisAdd
BLAST
Topological domaini184 – 21229CytoplasmicSequence analysisAdd
BLAST
Transmembranei213 – 23321HelicalSequence analysisAdd
BLAST
Topological domaini234 – 2429PeriplasmicSequence analysis
Transmembranei243 – 26321HelicalSequence analysisAdd
BLAST
Topological domaini264 – 27613CytoplasmicSequence analysisAdd
BLAST
Transmembranei277 – 29721HelicalSequence analysisAdd
BLAST
Topological domaini298 – 2981PeriplasmicSequence analysis
Transmembranei299 – 31921HelicalSequence analysisAdd
BLAST
Topological domaini320 – 32910CytoplasmicSequence analysis
Transmembranei330 – 35021HelicalSequence analysisAdd
BLAST
Topological domaini351 – 36414PeriplasmicSequence analysisAdd
BLAST
Transmembranei365 – 38521HelicalSequence analysisAdd
BLAST
Topological domaini386 – 3949CytoplasmicSequence analysis

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Multidrug resistance protein DPRO_0000173326Add
BLAST

Proteomic databases

PaxDbiP31442.

Interactioni

Protein-protein interaction databases

BioGridi4261242. 128 interactions.
STRINGi511145.b3673.

Structurei

Secondary structure

1
394
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 3526Combined sources
Beta strandi38 – 403Combined sources
Helixi43 – 6018Combined sources
Helixi63 – 675Combined sources
Helixi72 – 765Combined sources
Helixi77 – 12650Combined sources
Helixi133 – 15422Combined sources
Helixi158 – 17619Combined sources
Turni188 – 1903Combined sources
Turni196 – 1983Combined sources
Helixi202 – 22928Combined sources
Helixi235 – 25016Combined sources
Helixi252 – 26413Combined sources
Turni265 – 2684Combined sources
Helixi269 – 28315Combined sources
Beta strandi285 – 2895Combined sources
Helixi290 – 31526Combined sources
Helixi321 – 3255Combined sources
Helixi327 – 34822Combined sources
Helixi356 – 37722Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GFPX-ray3.50A/B9-383[»]
ProteinModelPortaliP31442.
SMRiP31442. Positions 20-383.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31442.

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105E5M. Bacteria.
ENOG410XQPV. LUCA.
HOGENOMiHOG000276020.
InParanoidiP31442.
KOiK08154.
OMAiGLNSMTV.
OrthoDBiEOG6RJV6T.
PhylomeDBiP31442.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRQRNVNLL LMLVLLVAVG QMAQTIYIPA IADMARDLNV REGAVQSVMG
60 70 80 90 100
AYLLTYGVSQ LFYGPISDRV GRRPVILVGM SIFMLATLVA VTTSSLTVLI
110 120 130 140 150
AASAMQGMGT GVGGVMARTL PRDLYERTQL RHANSLLNMG ILVSPLLAPL
160 170 180 190 200
IGGLLDTMWN WRACYLFLLV LCAGVTFSMA RWMPETRPVD APRTRLLTSY
210 220 230 240 250
KTLFGNSGFN CYLLMLIGGL AGIAAFEACS GVLMGAVLGL SSMTVSILFI
260 270 280 290 300
LPIPAAFFGA WFAGRPNKRF STLMWQSVIC CLLAGLLMWI PDWFGVMNVW
310 320 330 340 350
TLLVPAALFF FGAGMLFPLA TSGAMEPFPF LAGTAGALVG GLQNIGSGVL
360 370 380 390
ASLSAMLPQT GQGSLGLLMT LMGLLIVLCW LPLATRMSHQ GQPV
Length:394
Mass (Da):42,216
Last modified:April 27, 2001 - v2
Checksum:i342339DC7265A30B
GO

Sequence cautioni

The sequence AAA62025.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62025.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76696.2.
AP009048 Genomic DNA. Translation: BAE77620.1.
PIRiB65169.
RefSeqiNP_418129.2. NC_000913.3.
WP_000828746.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76696; AAC76696; b3673.
BAE77620; BAE77620; BAE77620.
GeneIDi948180.
KEGGiecj:JW5634.
eco:b3673.
PATRICi32122837. VBIEscCol129921_3794.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62025.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76696.2.
AP009048 Genomic DNA. Translation: BAE77620.1.
PIRiB65169.
RefSeqiNP_418129.2. NC_000913.3.
WP_000828746.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GFPX-ray3.50A/B9-383[»]
ProteinModelPortaliP31442.
SMRiP31442. Positions 20-383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261242. 128 interactions.
STRINGi511145.b3673.

Protein family/group databases

TCDBi2.A.1.2.9. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP31442.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76696; AAC76696; b3673.
BAE77620; BAE77620; BAE77620.
GeneIDi948180.
KEGGiecj:JW5634.
eco:b3673.
PATRICi32122837. VBIEscCol129921_3794.

Organism-specific databases

EchoBASEiEB1644.
EcoGeneiEG11693. emrD.

Phylogenomic databases

eggNOGiENOG4105E5M. Bacteria.
ENOG410XQPV. LUCA.
HOGENOMiHOG000276020.
InParanoidiP31442.
KOiK08154.
OMAiGLNSMTV.
OrthoDBiEOG6RJV6T.
PhylomeDBiP31442.

Enzyme and pathway databases

BioCyciEcoCyc:EMRD-MONOMER.
ECOL316407:JW5634-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP31442.
PROiP31442.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An E. coli gene emrD is involved in adaptation to low energy shock."
    Naroditskaya V., Schloseer M.J., Fan N.Y., Lewis K.
    Biochem. Biophys. Res. Commun. 196:803-809(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiEMRD_ECOLI
AccessioniPrimary (citable) accession number: P31442
Secondary accession number(s): Q2M7Y6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: April 27, 2001
Last modified: February 17, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.