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Protein

Adenine deaminase

Gene

ade

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Adenine + H2O = hypoxanthine + NH3.2 Publications

Cofactori

Mn2+2 Publications

pH dependencei

Optimum pH is 6.5-7.0.

Temperature dependencei

Optimum temperature is 60 degrees Celsius.

GO - Molecular functioni

  • adenine deaminase activity Source: EcoCyc
  • catalase activity Source: EcoCyc
  • ferrous iron binding Source: EcoCyc
  • manganese ion binding Source: EcoCyc

GO - Biological processi

  • adenine catabolic process Source: EcoliWiki
  • hypoxanthine biosynthetic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Manganese

Enzyme and pathway databases

BioCyciEcoCyc:EG11692-MONOMER.
ECOL316407:JW3640-MONOMER.
MetaCyc:EG11692-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine deaminase (EC:3.5.4.2)
Short name:
Adenase
Short name:
Adenine aminase
Gene namesi
Name:ade
Synonyms:yicP
Ordered Locus Names:b3665, JW3640
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11692. ade.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 588588Adenine deaminasePRO_0000142420Add
BLAST

Proteomic databases

PaxDbiP31441.
PRIDEiP31441.

2D gel databases

SWISS-2DPAGEP31441.

Expressioni

Inductioni

Repressed by H-NS. Activated by insertion of a variety of IS elements into a region extending from -145 to +13 relative to the transcription start site. IS elements essentially eliminate the H-NS-mediated silencing, but also stimulate ade expression 2-3 fold independently of the H-NS protein.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4262580. 9 interactions.
DIPiDIP-12439N.
IntActiP31441. 11 interactions.
MINTiMINT-1285289.
STRINGi511145.b3665.

Structurei

3D structure databases

ProteinModelPortaliP31441.
SMRiP31441. Positions 26-540.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the adenine deaminase family.Curated

Phylogenomic databases

eggNOGiENOG4105CPN. Bacteria.
COG1001. LUCA.
HOGENOMiHOG000276949.
InParanoidiP31441.
KOiK01486.
OMAiCTDDKHL.
PhylomeDBiP31441.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01518. Adenine_deamin. 1 hit.
InterProiIPR006679. Adenine_deam.
IPR026912. Adenine_deam_C.
IPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF13382. Adenine_deam_C. 1 hit.
PF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01178. ade. 1 hit.

Sequencei

Sequence statusi: Complete.

P31441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNSINHKFH HISRAEYQEL LAVSRGDAVA DYIIDNVSIL DLINGGEISG
60 70 80 90 100
PIVIKGRYIA GVGAEYTDAP ALQRIDARGA TAVPGFIDAH LHIESSMMTP
110 120 130 140 150
VTFETATLPR GLTTVICDPH EIVNVMGEAG FAWFARCAEQ ARQNQYLQVS
160 170 180 190 200
SCVPALEGCD VNGASFTLEQ MLAWRDHPQV TGLAEMMDYP GVISGQNALL
210 220 230 240 250
DKLDAFRHLT LDGHCPGLGG KELNAYITAG IENCHESYQL EEGRRKLQLG
260 270 280 290 300
MSLMIREGSA ARNLNALAPL INEFNSPQCM LCTDDRNPWE IAHEGHIDAL
310 320 330 340 350
IRRLIEQHNV PLHVAYRVAS WSTARHFGLN HLGLLAPGKQ ADIVLLSDAR
360 370 380 390 400
KVTVQQVLVK GEPIDAQTLQ AEESARLAQS APPYGNTIAR QPVSASDFAL
410 420 430 440 450
QFTPGKRYRV IDVIHNELIT HSHSSVYSEN GFDRDDVSFI AVLERYGQRL
460 470 480 490 500
APACGLLGGF GLNEGALAAT VSHDSHNIVV IGRSAEEMAL AVNQVIQDGG
510 520 530 540 550
GLCVVRNGQV QSHLPLPIAG LMSTDTAQSL AEQIDALKAA ARECGPLPDE
560 570 580
PFIQMAFLSL PVIPALKLTS QGLFDGEKFA FTTLEVTE
Length:588
Mass (Da):63,739
Last modified:November 1, 1997 - v2
Checksum:i2732C6B1B6595E48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62017.1.
U00096 Genomic DNA. Translation: AAC76688.1.
AP009048 Genomic DNA. Translation: BAE77628.1.
PIRiB65168.
RefSeqiNP_418121.1. NC_000913.3.
WP_001065718.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76688; AAC76688; b3665.
BAE77628; BAE77628; BAE77628.
GeneIDi948210.
KEGGiecj:JW3640.
eco:b3665.
PATRICi32122823. VBIEscCol129921_3787.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62017.1.
U00096 Genomic DNA. Translation: AAC76688.1.
AP009048 Genomic DNA. Translation: BAE77628.1.
PIRiB65168.
RefSeqiNP_418121.1. NC_000913.3.
WP_001065718.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP31441.
SMRiP31441. Positions 26-540.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262580. 9 interactions.
DIPiDIP-12439N.
IntActiP31441. 11 interactions.
MINTiMINT-1285289.
STRINGi511145.b3665.

2D gel databases

SWISS-2DPAGEP31441.

Proteomic databases

PaxDbiP31441.
PRIDEiP31441.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76688; AAC76688; b3665.
BAE77628; BAE77628; BAE77628.
GeneIDi948210.
KEGGiecj:JW3640.
eco:b3665.
PATRICi32122823. VBIEscCol129921_3787.

Organism-specific databases

EchoBASEiEB1643.
EcoGeneiEG11692. ade.

Phylogenomic databases

eggNOGiENOG4105CPN. Bacteria.
COG1001. LUCA.
HOGENOMiHOG000276949.
InParanoidiP31441.
KOiK01486.
OMAiCTDDKHL.
PhylomeDBiP31441.

Enzyme and pathway databases

BioCyciEcoCyc:EG11692-MONOMER.
ECOL316407:JW3640-MONOMER.
MetaCyc:EG11692-MONOMER.

Miscellaneous databases

PROiP31441.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01518. Adenine_deamin. 1 hit.
InterProiIPR006679. Adenine_deam.
IPR026912. Adenine_deam_C.
IPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF13382. Adenine_deam_C. 1 hit.
PF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01178. ade. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADEC_ECOLI
AccessioniPrimary (citable) accession number: P31441
Secondary accession number(s): P78121, Q2M7X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Ade is a cryptic gene in wild-type strains. The physiological conditions that lead to its activation are unknown.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.