Reviewed, UniProtKB/Swiss-Prot P31441 (ADEC_ECOLI)

Names and origin

Protein names

Recommended name:
    Adenine deaminase
      Short name=Adenase
      Short name=Adenine aminase
    EC=3.5.4.2

Gene names

Name:	ade
Synonyms:	yicP
Ordered Locus Names:	b3665, JW3640

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Adenine + H2O = hypoxanthine + NH3. Ref.4 Ref.5
Cofactor	Manganese. Ref.4 Ref.5
Subunit structure	Homodimer. Ref.4
Induction	Repressed by H-NS. Activated by insertion of a variety of IS elements into a region extending from -145 to +13 relative to the transcription start site. IS elements essentially eliminate the H-NS-mediated silencing, but also stimulate ade expression 2-3 fold independently of the H-NS protein. Ref.5
Miscellaneous	Ade is a cryptic gene in wild-type strains. The physiological conditions that lead to its activation are unknown. HAMAP MF_01518
Sequence similarities	Belongs to the adenine deaminase family.
biophysicochemical properties	pH dependence: Optimum pH is 6.5-7.0. HAMAP MF_01518 Temperature dependence: Optimum temperature is 60 degrees Celsius.

Ontologies

Keywords
Ligand	Manganese
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	adenine catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenine deaminase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Adenine deaminase HAMAP MF_01518		PRO_0000142420

Sequences

	Sequence		Length	Mass (Da)
	P31441-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 2732C6B1B6595E48	FASTA	588	63,739
	10 20 30 40 50 60 MNNSINHKFH HISRAEYQEL LAVSRGDAVA DYIIDNVSIL DLINGGEISG PIVIKGRYIA 70 80 90 100 110 120 GVGAEYTDAP ALQRIDARGA TAVPGFIDAH LHIESSMMTP VTFETATLPR GLTTVICDPH 130 140 150 160 170 180 EIVNVMGEAG FAWFARCAEQ ARQNQYLQVS SCVPALEGCD VNGASFTLEQ MLAWRDHPQV 190 200 210 220 230 240 TGLAEMMDYP GVISGQNALL DKLDAFRHLT LDGHCPGLGG KELNAYITAG IENCHESYQL 250 260 270 280 290 300 EEGRRKLQLG MSLMIREGSA ARNLNALAPL INEFNSPQCM LCTDDRNPWE IAHEGHIDAL 310 320 330 340 350 360 IRRLIEQHNV PLHVAYRVAS WSTARHFGLN HLGLLAPGKQ ADIVLLSDAR KVTVQQVLVK 370 380 390 400 410 420 GEPIDAQTLQ AEESARLAQS APPYGNTIAR QPVSASDFAL QFTPGKRYRV IDVIHNELIT 430 440 450 460 470 480 HSHSSVYSEN GFDRDDVSFI AVLERYGQRL APACGLLGGF GLNEGALAAT VSHDSHNIVV 490 500 510 520 530 540 IGRSAEEMAL AVNQVIQDGG GLCVVRNGQV QSHLPLPIAG LMSTDTAQSL AEQIDALKAA 550 560 570 580 ARECGPLPDE PFIQMAFLSL PVIPALKLTS QGLFDGEKFA FTTLEVTE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed: 7686882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Adenine deaminase activity of the yicP gene product of Escherichia coli." Matsui H., Shimaoka M., Kawasaki H., Takenaka Y., Kurahashi O. Biosci. Biotechnol. Biochem. 65:1112-1118(2001) [PubMed: 11440125] [Abstract] Cited for: CATALYTIC ACTIVITY, COFACTOR, SUBUNIT. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The cryptic adenine deaminase gene of Escherichia coli. Silencing by the nucleoid-associated DNA-binding protein, H-NS, and activation by insertion elements." Petersen C., Moeller L.B., Valentin-Hansen P. J. Biol. Chem. 277:31373-31380(2002) [PubMed: 12077137] [Abstract] Cited for: CATALYTIC ACTIVITY, COFACTOR, INDUCTION. Strain: K12.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L10328 Genomic DNA. Translation: AAA62017.1. U00096 Genomic DNA. Translation: AAC76688.1. AP009048 Genomic DNA. Translation: BAE77628.1.
PIR	B65168.
RefSeq	AP_004127.1. NP_418121.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:12439N.
2-D gel databases
SWISS-2DPAGE	P31441.
Genome annotation databases
GeneID	948210.
GenomeReviews	Gene locus JW3640 in contig AP009048_GR. Gene locus b3665 in contig U00096_GR.
KEGG	ecj:JW3640. eco:b3665.
Organism-specific databases
EchoBASE	EB1643.
EcoGene	EG11692. ade.
CMR	Search...
Phylogenomic databases
HOGENOM	P31441.
OMA	P31441. IMIREGS.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11692-MON. MetaCyc:EG11692-MON.
Family and domain databases
HAMAP	MF_01518. [Tree]
InterPro	IPR006679. Adenine_deam. IPR006680. Amidohydro_1. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01178. ade. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ADEC_ECOLI

Accession

Primary (citable) accession number: P31441
Secondary accession number(s): P78121, Q2M7X8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1997
Last modified:	May 5, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)