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Protein

Alpha-xylosidase

Gene

yicI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Can catalyze the transfer of alpha-xylosyl residue from alpha-xyloside to xylose, glucose, mannose, fructose, maltose, isomaltose, nigerose, kojibiose, sucrose and trehalose.2 Publications

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-xylose residues with release of alpha-D-xylose.1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei416Nucleophile1 Publication1
Active sitei4191 Publication1
Active sitei482Proton donor1 Publication1

GO - Molecular functioni

  • alpha-D-xyloside xylohydrolase Source: UniProtKB-EC
  • carbohydrate binding Source: InterPro
  • identical protein binding Source: EcoCyc
  • xyloglucan 1,6-alpha-xylosidase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG11685-MONOMER.
ECOL316407:JW3631-MONOMER.
MetaCyc:EG11685-MONOMER.
BRENDAi3.2.1.177. 2026.
SABIO-RKP31434.

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-xylosidase (EC:3.2.1.177)
Gene namesi
Name:yicI
Ordered Locus Names:b3656, JW3631
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11685. yicI.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi307 – 308CF → ID: Converts the enzyme to have alpha-glucosidase activity. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001853711 – 772Alpha-xylosidaseAdd BLAST772

Proteomic databases

PaxDbiP31434.
PRIDEiP31434.

Interactioni

Subunit structurei

Homohexamer.2 Publications

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4262572. 5 interactors.
DIPiDIP-12433N.
IntActiP31434. 2 interactors.
STRINGi511145.b3656.

Structurei

Secondary structure

1772
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 8Combined sources3
Beta strandi14 – 17Combined sources4
Beta strandi21 – 28Combined sources8
Beta strandi31 – 39Combined sources9
Helixi44 – 46Combined sources3
Beta strandi47 – 50Combined sources4
Beta strandi53 – 58Combined sources6
Beta strandi64 – 71Combined sources8
Beta strandi92 – 96Combined sources5
Beta strandi98 – 105Combined sources8
Beta strandi108 – 113Combined sources6
Beta strandi115 – 117Combined sources3
Beta strandi119 – 124Combined sources6
Beta strandi127 – 133Combined sources7
Beta strandi136 – 142Combined sources7
Turni143 – 146Combined sources4
Beta strandi147 – 155Combined sources9
Beta strandi162 – 167Combined sources6
Beta strandi177 – 180Combined sources4
Beta strandi189 – 191Combined sources3
Beta strandi194 – 202Combined sources9
Beta strandi205 – 210Combined sources6
Beta strandi217 – 224Combined sources8
Beta strandi227 – 243Combined sources17
Helixi247 – 258Combined sources12
Helixi266 – 269Combined sources4
Beta strandi270 – 274Combined sources5
Beta strandi277 – 279Combined sources3
Helixi283 – 295Combined sources13
Beta strandi302 – 305Combined sources4
Helixi307 – 309Combined sources3
Turni322 – 324Combined sources3
Helixi328 – 337Combined sources10
Beta strandi341 – 346Combined sources6
Beta strandi348 – 350Combined sources3
Helixi357 – 363Combined sources7
Beta strandi372 – 374Combined sources3
Beta strandi377 – 381Combined sources5
Beta strandi384 – 387Combined sources4
Helixi392 – 407Combined sources16
Beta strandi412 – 415Combined sources4
Beta strandi423 – 426Combined sources4
Helixi433 – 453Combined sources21
Turni454 – 456Combined sources3
Helixi458 – 460Combined sources3
Beta strandi463 – 466Combined sources4
Helixi472 – 474Combined sources3
Beta strandi484 – 486Combined sources3
Helixi487 – 501Combined sources15
Turni502 – 504Combined sources3
Beta strandi508 – 511Combined sources4
Beta strandi515 – 517Combined sources3
Helixi521 – 532Combined sources12
Beta strandi534 – 539Combined sources6
Beta strandi542 – 544Combined sources3
Helixi548 – 550Combined sources3
Helixi553 – 583Combined sources31
Beta strandi587 – 589Combined sources3
Helixi591 – 594Combined sources4
Helixi599 – 601Combined sources3
Beta strandi608 – 610Combined sources3
Turni611 – 613Combined sources3
Beta strandi614 – 616Combined sources3
Beta strandi621 – 623Combined sources3
Beta strandi625 – 630Combined sources6
Beta strandi632 – 637Combined sources6
Turni638 – 640Combined sources3
Beta strandi643 – 645Combined sources3
Beta strandi647 – 653Combined sources7
Beta strandi661 – 663Combined sources3
Beta strandi665 – 671Combined sources7
Beta strandi678 – 680Combined sources3
Beta strandi687 – 691Combined sources5
Beta strandi698 – 704Combined sources7
Beta strandi710 – 719Combined sources10
Beta strandi722 – 729Combined sources8
Beta strandi735 – 738Combined sources4
Beta strandi745 – 753Combined sources9
Beta strandi756 – 758Combined sources3
Beta strandi760 – 766Combined sources7
Beta strandi770 – 772Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WE5X-ray2.40A/B/C/D/E/F1-772[»]
1XSIX-ray2.20A/B/C/D/E/F1-772[»]
1XSJX-ray2.10A/B/C/D/E/F1-772[»]
1XSKX-ray2.20A/B/C/D/E/F1-772[»]
2F2HX-ray1.95A/B/C/D/E/F1-772[»]
ProteinModelPortaliP31434.
SMRiP31434.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31434.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Phylogenomic databases

eggNOGiENOG4105CJQ. Bacteria.
COG1501. LUCA.
HOGENOMiHOG000221907.
InParanoidiP31434.
KOiK01811.
OMAiWIYKRWV.
PhylomeDBiP31434.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.

Sequencei

Sequence statusi: Complete.

P31434-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKISDGNWLI QPGLNLIHPL QVFEVEQQDN EMVVYAAPRD VRERTWQLDT
60 70 80 90 100
PLFTLRFFSP QEGIVGVRIE HFQGALNNGP HYPLNILQDV KVTIENTERY
110 120 130 140 150
AEFKSGNLSA RVSKGEFWSL DFLRNGERIT GSQVKNNGYV QDTNNQRNYM
160 170 180 190 200
FERLDLGVGE TVYGLGERFT ALVRNGQTVE TWNRDGGTST EQAYKNIPFY
210 220 230 240 250
MTNRGYGVLV NHPQCVSFEV GSEKVSKVQF SVESEYLEYF VIDGPTPKAV
260 270 280 290 300
LDRYTRFTGR PALPPAWSFG LWLTTSFTTN YDEATVNSFI DGMAERNLPL
310 320 330 340 350
HVFHFDCFWM KAFQWCDFEW DPLTFPDPEG MIRRLKAKGL KICVWINPYI
360 370 380 390 400
GQKSPVFKEL QEKGYLLKRP DGSLWQWDKW QPGLAIYDFT NPDACKWYAD
410 420 430 440 450
KLKGLVAMGV DCFKTDFGER IPTDVQWFDG SDPQKMHNHY AYIYNELVWN
460 470 480 490 500
VLKDTVGEEE AVLFARSASV GAQKFPVHWG GDCYANYESM AESLRGGLSI
510 520 530 540 550
GLSGFGFWSH DIGGFENTAP AHVYKRWCAF GLLSSHSRLH GSKSYRVPWA
560 570 580 590 600
YDDESCDVVR FFTQLKCRMM PYLYREAARA NARGTPMMRA MMMEFPDDPA
610 620 630 640 650
CDYLDRQYML GDNVMVAPVF TEAGDVQFYL PEGRWTHLWH NDELDGSRWH
660 670 680 690 700
KQQHGFLSLP VYVRDNTLLA LGNNDQRPDY VWHEGTAFHL FNLQDGHEAV
710 720 730 740 750
CEVPAADGSV IFTLKAARTG NTITVTGAGE AKNWTLCLRN VVKVNGLQDG
760 770
SQAESEQGLV VKPQGNALTI TL
Length:772
Mass (Da):88,079
Last modified:July 15, 1998 - v2
Checksum:i6F2A02E4B5403772
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62009.1.
U00096 Genomic DNA. Translation: AAC76680.1.
AP009048 Genomic DNA. Translation: BAE77637.1.
PIRiB65167.
RefSeqiNP_418113.1. NC_000913.3.
WP_000702898.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76680; AAC76680; b3656.
BAE77637; BAE77637; BAE77637.
GeneIDi948169.
KEGGiecj:JW3631.
eco:b3656.
PATRICi32122799. VBIEscCol129921_3776.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA. Translation: AAA62009.1.
U00096 Genomic DNA. Translation: AAC76680.1.
AP009048 Genomic DNA. Translation: BAE77637.1.
PIRiB65167.
RefSeqiNP_418113.1. NC_000913.3.
WP_000702898.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WE5X-ray2.40A/B/C/D/E/F1-772[»]
1XSIX-ray2.20A/B/C/D/E/F1-772[»]
1XSJX-ray2.10A/B/C/D/E/F1-772[»]
1XSKX-ray2.20A/B/C/D/E/F1-772[»]
2F2HX-ray1.95A/B/C/D/E/F1-772[»]
ProteinModelPortaliP31434.
SMRiP31434.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262572. 5 interactors.
DIPiDIP-12433N.
IntActiP31434. 2 interactors.
STRINGi511145.b3656.

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Proteomic databases

PaxDbiP31434.
PRIDEiP31434.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76680; AAC76680; b3656.
BAE77637; BAE77637; BAE77637.
GeneIDi948169.
KEGGiecj:JW3631.
eco:b3656.
PATRICi32122799. VBIEscCol129921_3776.

Organism-specific databases

EchoBASEiEB1636.
EcoGeneiEG11685. yicI.

Phylogenomic databases

eggNOGiENOG4105CJQ. Bacteria.
COG1501. LUCA.
HOGENOMiHOG000221907.
InParanoidiP31434.
KOiK01811.
OMAiWIYKRWV.
PhylomeDBiP31434.

Enzyme and pathway databases

BioCyciEcoCyc:EG11685-MONOMER.
ECOL316407:JW3631-MONOMER.
MetaCyc:EG11685-MONOMER.
BRENDAi3.2.1.177. 2026.
SABIO-RKP31434.

Miscellaneous databases

EvolutionaryTraceiP31434.
PROiP31434.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
InterProiIPR013785. Aldolase_TIM.
IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiXYLS_ECOLI
AccessioniPrimary (citable) accession number: P31434
Secondary accession number(s): P76723, Q2M7W9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.