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Protein

Dipeptidase 1

Gene

Dpep1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity.

Catalytic activityi

Hydrolysis of dipeptides.PROSITE-ProRule annotation

Cofactori

Enzyme regulationi

Inhibited by L-penicillamine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Zinc 1; catalyticPROSITE-ProRule annotation
Metal bindingi38 – 381Zinc 1; catalyticPROSITE-ProRule annotation
Metal bindingi141 – 1411Zinc 1; catalyticPROSITE-ProRule annotation
Metal bindingi141 – 1411Zinc 2; catalyticPROSITE-ProRule annotation
Binding sitei168 – 1681SubstratePROSITE-ProRule annotation
Metal bindingi214 – 2141Zinc 2; catalyticPROSITE-ProRule annotation
Metal bindingi235 – 2351Zinc 2; catalyticPROSITE-ProRule annotation
Binding sitei246 – 2461SubstratePROSITE-ProRule annotation
Binding sitei304 – 3041SubstratePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dipeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_278052. Aflatoxin activation and detoxification.
REACT_325114. Synthesis of Leukotrienes (LT) and Eoxins (EX).
SABIO-RKP31430.

Protein family/group databases

MEROPSiM19.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Dipeptidase 1 (EC:3.4.13.19)
Alternative name(s):
Microsomal dipeptidase
Renal dipeptidase
Gene namesi
Name:Dpep1
Synonyms:Rdp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi620324. Dpep1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Add
BLAST
Chaini17 – 384368Dipeptidase 1PRO_0000018660Add
BLAST
Propeptidei385 – 41026Removed in mature formBy similarityPRO_0000018661Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)By similarity
Disulfide bondi87 ↔ 170PROSITE-ProRule annotation
Glycosylationi121 – 1211N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi242 ↔ 274PROSITE-ProRule annotation
Glycosylationi332 – 3321N-linked (GlcNAc...)By similarity
Disulfide bondi377 – 377InterchainPROSITE-ProRule annotation
Lipidationi384 – 3841GPI-anchor amidated serineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP31430.
PRIDEiP31430.

PTM databases

PhosphoSiteiP31430.

Expressioni

Gene expression databases

GenevisibleiP31430. RN.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021397.

Structurei

3D structure databases

ProteinModelPortaliP31430.
SMRiP31430. Positions 17-384.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M19 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2355.
GeneTreeiENSGT00390000017920.
HOGENOMiHOG000072016.
HOVERGENiHBG002339.
InParanoidiP31430.
KOiK01273.
OMAiASHSNCY.
OrthoDBiEOG7SJD4N.
PhylomeDBiP31430.
TreeFamiTF324523.

Family and domain databases

InterProiIPR000180. Dipep_AS.
IPR028536. Dpep1.
IPR008257. Renal_dipep_fam.
[Graphical view]
PANTHERiPTHR10443. PTHR10443. 1 hit.
PTHR10443:SF17. PTHR10443:SF17. 1 hit.
PfamiPF01244. Peptidase_M19. 1 hit.
[Graphical view]
PROSITEiPS00869. RENAL_DIPEPTIDASE_1. 1 hit.
PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIIWWFWSL LAICASDSFR NQAENIMRTT PVIDGHNDLP WQMLTLFNNQ
60 70 80 90 100
LRKSEANLSA LAETHTNIPK LRAGFVGGQF WSAYMPCDTQ NKDAVKRILE
110 120 130 140 150
QIDVIHRMCQ LYPETFECVT NSSDILQAFR RGKVASLIGV EGGHLIDSSL
160 170 180 190 200
GVLRTLYHLG MRYLTLTHNC NTPWADNWLV DKGDDEAESQ GLSPFGKLVL
210 220 230 240 250
NEMNRLGVMI DLSHVSVATM KDALQLSKAP VIFSHSSAYS VCPHRRNVPD
260 270 280 290 300
DVLQLVKSTN SLVMVNFYNQ FVSCSDSATL SQVADHLDHI KKVAGAGAVG
310 320 330 340 350
LGGDYDGVTN LPVGLEDVSK YPDLIAELLR RNWTETEVRG LLAENLLRVF
360 370 380 390 400
SAVELVSNIM QVPEEETIPV EKLDGSCRTF YGHSRAPSIH LQIGALLASL
410
ASLVFSLHLL
Length:410
Mass (Da):45,522
Last modified:May 1, 2007 - v2
Checksum:i6DD618B44523862C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti409 – 4091L → P in AAA41093 (PubMed:1420313).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94056 mRNA. Translation: AAA41093.1.
L07315 mRNA. Translation: AAA41094.1.
L07316 mRNA. Translation: AAA41095.1.
BC072476 mRNA. Translation: AAH72476.1.
PIRiS27204.
RefSeqiNP_446043.1. NM_053591.2.
XP_006255845.1. XM_006255783.2.
UniGeneiRn.6051.

Genome annotation databases

EnsembliENSRNOT00000021397; ENSRNOP00000021397; ENSRNOG00000015880.
GeneIDi94199.
KEGGirno:94199.
UCSCiRGD:620324. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94056 mRNA. Translation: AAA41093.1.
L07315 mRNA. Translation: AAA41094.1.
L07316 mRNA. Translation: AAA41095.1.
BC072476 mRNA. Translation: AAH72476.1.
PIRiS27204.
RefSeqiNP_446043.1. NM_053591.2.
XP_006255845.1. XM_006255783.2.
UniGeneiRn.6051.

3D structure databases

ProteinModelPortaliP31430.
SMRiP31430. Positions 17-384.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021397.

Protein family/group databases

MEROPSiM19.001.

PTM databases

PhosphoSiteiP31430.

Proteomic databases

PaxDbiP31430.
PRIDEiP31430.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021397; ENSRNOP00000021397; ENSRNOG00000015880.
GeneIDi94199.
KEGGirno:94199.
UCSCiRGD:620324. rat.

Organism-specific databases

CTDi1800.
RGDi620324. Dpep1.

Phylogenomic databases

eggNOGiCOG2355.
GeneTreeiENSGT00390000017920.
HOGENOMiHOG000072016.
HOVERGENiHBG002339.
InParanoidiP31430.
KOiK01273.
OMAiASHSNCY.
OrthoDBiEOG7SJD4N.
PhylomeDBiP31430.
TreeFamiTF324523.

Enzyme and pathway databases

ReactomeiREACT_278052. Aflatoxin activation and detoxification.
REACT_325114. Synthesis of Leukotrienes (LT) and Eoxins (EX).
SABIO-RKP31430.

Miscellaneous databases

NextBioi617866.
PROiP31430.

Gene expression databases

GenevisibleiP31430. RN.

Family and domain databases

InterProiIPR000180. Dipep_AS.
IPR028536. Dpep1.
IPR008257. Renal_dipep_fam.
[Graphical view]
PANTHERiPTHR10443. PTHR10443. 1 hit.
PTHR10443:SF17. PTHR10443:SF17. 1 hit.
PfamiPF01244. Peptidase_M19. 1 hit.
[Graphical view]
PROSITEiPS00869. RENAL_DIPEPTIDASE_1. 1 hit.
PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of rat renal dipeptidase and expression of its mRNA in rat tissues and COS-1 cells."
    Adachi H., Ishida N., Tsujimoto M.
    Biochim. Biophys. Acta 1132:311-314(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiDPEP1_RAT
AccessioniPrimary (citable) accession number: P31430
Secondary accession number(s): Q6IN35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 1, 2007
Last modified: June 24, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.