Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P31430

- DPEP1_RAT

UniProt

P31430 - DPEP1_RAT

Protein

Dipeptidase 1

Gene

Dpep1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity.

    Catalytic activityi

    Hydrolysis of dipeptides.PROSITE-ProRule annotation

    Cofactori

    Zinc.

    Enzyme regulationi

    Inhibited by L-penicillamine.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi36 – 361Zinc 1; catalyticPROSITE-ProRule annotation
    Metal bindingi38 – 381Zinc 1; catalyticPROSITE-ProRule annotation
    Metal bindingi141 – 1411Zinc 1; catalyticPROSITE-ProRule annotation
    Metal bindingi141 – 1411Zinc 2; catalyticPROSITE-ProRule annotation
    Binding sitei168 – 1681SubstratePROSITE-ProRule annotation
    Metal bindingi214 – 2141Zinc 2; catalyticPROSITE-ProRule annotation
    Metal bindingi235 – 2351Zinc 2; catalyticPROSITE-ProRule annotation
    Binding sitei246 – 2461SubstratePROSITE-ProRule annotation
    Binding sitei304 – 3041SubstratePROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type endopeptidase inhibitor activity involved in apoptotic process Source: UniProtKB
    2. dipeptidase activity Source: RGD
    3. dipeptidyl-peptidase activity Source: InterPro
    4. GPI anchor binding Source: UniProtKB
    5. metallodipeptidase activity Source: UniProtKB
    6. modified amino acid binding Source: UniProtKB
    7. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. antibiotic metabolic process Source: UniProtKB
    2. cellular response to calcium ion Source: UniProtKB
    3. cellular response to drug Source: UniProtKB
    4. cellular response to nitric oxide Source: UniProtKB
    5. homocysteine metabolic process Source: UniProtKB
    6. negative regulation of apoptotic process Source: UniProtKB
    7. negative regulation of cell migration Source: UniProtKB
    8. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB

    Keywords - Molecular functioni

    Dipeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_197237. Synthesis of Leukotrienes (LT) and Eoxins (EX).
    SABIO-RKP31430.

    Protein family/group databases

    MEROPSiM19.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dipeptidase 1 (EC:3.4.13.19)
    Alternative name(s):
    Microsomal dipeptidase
    Renal dipeptidase
    Gene namesi
    Name:Dpep1
    Synonyms:Rdp
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 19

    Organism-specific databases

    RGDi620324. Dpep1.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. apical part of cell Source: UniProtKB
    3. apical plasma membrane Source: UniProtKB-SubCell
    4. extracellular space Source: UniProtKB
    5. extracellular vesicular exosome Source: Ensembl
    6. microvillus membrane Source: UniProtKB-SubCell
    7. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Add
    BLAST
    Chaini17 – 384368Dipeptidase 1PRO_0000018660Add
    BLAST
    Propeptidei385 – 41026Removed in mature formBy similarityPRO_0000018661Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi57 – 571N-linked (GlcNAc...)By similarity
    Disulfide bondi87 ↔ 170PROSITE-ProRule annotation
    Glycosylationi121 – 1211N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi242 ↔ 274PROSITE-ProRule annotation
    Glycosylationi332 – 3321N-linked (GlcNAc...)By similarity
    Disulfide bondi377 – 377InterchainPROSITE-ProRule annotation
    Lipidationi384 – 3841GPI-anchor amidated serineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiP31430.
    PRIDEiP31430.

    PTM databases

    PhosphoSiteiP31430.

    Expressioni

    Gene expression databases

    GenevestigatoriP31430.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000021397.

    Structurei

    3D structure databases

    ProteinModelPortaliP31430.
    SMRiP31430. Positions 17-384.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M19 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2355.
    GeneTreeiENSGT00390000017920.
    HOGENOMiHOG000072016.
    HOVERGENiHBG002339.
    InParanoidiP31430.
    KOiK01273.
    OMAiGHYATAM.
    OrthoDBiEOG7SJD4N.
    PhylomeDBiP31430.
    TreeFamiTF324523.

    Family and domain databases

    InterProiIPR000180. Dipep_AS.
    IPR028536. Dpep1.
    IPR008257. Renal_dipep_fam.
    [Graphical view]
    PANTHERiPTHR10443. PTHR10443. 1 hit.
    PTHR10443:SF17. PTHR10443:SF17. 1 hit.
    PfamiPF01244. Peptidase_M19. 1 hit.
    [Graphical view]
    PROSITEiPS00869. RENAL_DIPEPTIDASE_1. 1 hit.
    PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31430-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLIIWWFWSL LAICASDSFR NQAENIMRTT PVIDGHNDLP WQMLTLFNNQ    50
    LRKSEANLSA LAETHTNIPK LRAGFVGGQF WSAYMPCDTQ NKDAVKRILE 100
    QIDVIHRMCQ LYPETFECVT NSSDILQAFR RGKVASLIGV EGGHLIDSSL 150
    GVLRTLYHLG MRYLTLTHNC NTPWADNWLV DKGDDEAESQ GLSPFGKLVL 200
    NEMNRLGVMI DLSHVSVATM KDALQLSKAP VIFSHSSAYS VCPHRRNVPD 250
    DVLQLVKSTN SLVMVNFYNQ FVSCSDSATL SQVADHLDHI KKVAGAGAVG 300
    LGGDYDGVTN LPVGLEDVSK YPDLIAELLR RNWTETEVRG LLAENLLRVF 350
    SAVELVSNIM QVPEEETIPV EKLDGSCRTF YGHSRAPSIH LQIGALLASL 400
    ASLVFSLHLL 410
    Length:410
    Mass (Da):45,522
    Last modified:May 1, 2007 - v2
    Checksum:i6DD618B44523862C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti409 – 4091L → P in AAA41093. (PubMed:1420313)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94056 mRNA. Translation: AAA41093.1.
    L07315 mRNA. Translation: AAA41094.1.
    L07316 mRNA. Translation: AAA41095.1.
    BC072476 mRNA. Translation: AAH72476.1.
    PIRiS27204.
    RefSeqiNP_446043.1. NM_053591.2.
    XP_006255845.1. XM_006255783.1.
    XP_006255846.1. XM_006255784.1.
    UniGeneiRn.6051.

    Genome annotation databases

    EnsembliENSRNOT00000021397; ENSRNOP00000021397; ENSRNOG00000015880.
    GeneIDi94199.
    KEGGirno:94199.
    UCSCiRGD:620324. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94056 mRNA. Translation: AAA41093.1 .
    L07315 mRNA. Translation: AAA41094.1 .
    L07316 mRNA. Translation: AAA41095.1 .
    BC072476 mRNA. Translation: AAH72476.1 .
    PIRi S27204.
    RefSeqi NP_446043.1. NM_053591.2.
    XP_006255845.1. XM_006255783.1.
    XP_006255846.1. XM_006255784.1.
    UniGenei Rn.6051.

    3D structure databases

    ProteinModelPortali P31430.
    SMRi P31430. Positions 17-384.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000021397.

    Protein family/group databases

    MEROPSi M19.001.

    PTM databases

    PhosphoSitei P31430.

    Proteomic databases

    PaxDbi P31430.
    PRIDEi P31430.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000021397 ; ENSRNOP00000021397 ; ENSRNOG00000015880 .
    GeneIDi 94199.
    KEGGi rno:94199.
    UCSCi RGD:620324. rat.

    Organism-specific databases

    CTDi 1800.
    RGDi 620324. Dpep1.

    Phylogenomic databases

    eggNOGi COG2355.
    GeneTreei ENSGT00390000017920.
    HOGENOMi HOG000072016.
    HOVERGENi HBG002339.
    InParanoidi P31430.
    KOi K01273.
    OMAi GHYATAM.
    OrthoDBi EOG7SJD4N.
    PhylomeDBi P31430.
    TreeFami TF324523.

    Enzyme and pathway databases

    Reactomei REACT_197237. Synthesis of Leukotrienes (LT) and Eoxins (EX).
    SABIO-RK P31430.

    Miscellaneous databases

    NextBioi 617866.
    PROi P31430.

    Gene expression databases

    Genevestigatori P31430.

    Family and domain databases

    InterProi IPR000180. Dipep_AS.
    IPR028536. Dpep1.
    IPR008257. Renal_dipep_fam.
    [Graphical view ]
    PANTHERi PTHR10443. PTHR10443. 1 hit.
    PTHR10443:SF17. PTHR10443:SF17. 1 hit.
    Pfami PF01244. Peptidase_M19. 1 hit.
    [Graphical view ]
    PROSITEi PS00869. RENAL_DIPEPTIDASE_1. 1 hit.
    PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of rat renal dipeptidase and expression of its mRNA in rat tissues and COS-1 cells."
      Adachi H., Ishida N., Tsujimoto M.
      Biochim. Biophys. Acta 1132:311-314(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.

    Entry informationi

    Entry nameiDPEP1_RAT
    AccessioniPrimary (citable) accession number: P31430
    Secondary accession number(s): Q6IN35
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3