SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P31428

- DPEP1_MOUSE

UniProt

P31428 - DPEP1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Dipeptidase 1
Gene
Dpep1, Mbd1, Rdp
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity.

Catalytic activityi

Hydrolysis of dipeptides.

Cofactori

Zinc.

Enzyme regulationi

Inhibited by L-penicillamine.1 Publication

Kineticsi

  1. KM=10 µM for leukotriene D41 Publication
  2. KM=0.45 mM for cystinyl-bis-glycine
  3. KM=111 µM for beta-lactam

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Zinc 1; catalytic By similarity
Metal bindingi38 – 381Zinc 1; catalytic By similarity
Metal bindingi141 – 1411Zinc 1; catalytic By similarity
Metal bindingi141 – 1411Zinc 2; catalytic By similarity
Binding sitei168 – 1681Substrate By similarity
Metal bindingi214 – 2141Zinc 2; catalytic By similarity
Metal bindingi235 – 2351Zinc 2; catalytic By similarity
Binding sitei246 – 2461Substrate By similarity
Binding sitei304 – 3041Substrate By similarity

GO - Molecular functioni

  1. GPI anchor binding Source: UniProtKB
  2. cysteine-type endopeptidase inhibitor activity involved in apoptotic process Source: UniProtKB
  3. dipeptidyl-peptidase activity Source: InterPro
  4. metallodipeptidase activity Source: UniProtKB
  5. modified amino acid binding Source: UniProtKB
  6. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. antibiotic metabolic process Source: UniProtKB
  2. cellular response to calcium ion Source: UniProtKB
  3. cellular response to drug Source: UniProtKB
  4. cellular response to nitric oxide Source: UniProtKB
  5. homocysteine metabolic process Source: UniProtKB
  6. negative regulation of apoptotic process Source: UniProtKB
  7. negative regulation of cell migration Source: UniProtKB
  8. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dipeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_215561. Synthesis of Leukotrienes (LT) and Eoxins (EX).

Protein family/group databases

MEROPSiM19.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Dipeptidase 1 (EC:3.4.13.19)
Alternative name(s):
Membrane-bound dipeptidase 1
Short name:
MBD-1
Microsomal dipeptidase
Renal dipeptidase
Gene namesi
Name:Dpep1
Synonyms:Mbd1, Rdp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:94917. Dpep1.

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. apical part of cell Source: UniProtKB
  3. apical plasma membrane Source: UniProtKB-SubCell
  4. extracellular space Source: UniProtKB
  5. extracellular vesicular exosome Source: Ensembl
  6. microvillus membrane Source: UniProtKB-SubCell
  7. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616 By similarity
Add
BLAST
Chaini17 – 384368Dipeptidase 1
PRO_0000018654Add
BLAST
Propeptidei385 – 41026Removed in mature form By similarity
PRO_0000018655Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi87 ↔ 170 By similarity
Glycosylationi121 – 1211N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi242 ↔ 274 By similarity
Glycosylationi258 – 2581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi332 – 3321N-linked (GlcNAc...) By similarity
Disulfide bondi377 – 377Interchain By similarity
Lipidationi384 – 3841GPI-anchor amidated serine By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP31428.
PRIDEiP31428.

PTM databases

PhosphoSiteiP31428.

Expressioni

Gene expression databases

CleanExiMM_DPEP1.
MM_MBD1.
GenevestigatoriP31428.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Protein-protein interaction databases

IntActiP31428. 3 interactions.
MINTiMINT-4101665.
STRINGi10090.ENSMUSP00000019422.

Structurei

3D structure databases

ProteinModelPortaliP31428.
SMRiP31428. Positions 17-384.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M19 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2355.
GeneTreeiENSGT00390000017920.
HOGENOMiHOG000072016.
HOVERGENiHBG002339.
InParanoidiP31428.
KOiK01273.
OMAiGMRYMTL.
OrthoDBiEOG7SJD4N.
TreeFamiTF324523.

Family and domain databases

InterProiIPR000180. Dipep_AS.
IPR028536. Dpep1.
IPR008257. Renal_dipep_fam.
[Graphical view]
PANTHERiPTHR10443. PTHR10443. 1 hit.
PTHR10443:SF17. PTHR10443:SF17. 1 hit.
PfamiPF01244. Peptidase_M19. 1 hit.
[Graphical view]
PROSITEiPS00869. RENAL_DIPEPTIDASE_1. 1 hit.
PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31428-1 [UniParc]FASTAAdd to Basket

« Hide

MVIIWWFWSL LAICASDSFR DQAVAIMRTT PVIDGHNDLP WQLLNLFNNQ    50
LLRPDADLNK LAQTHTNIPK LKAGFVGGQF WSAYMPCDTQ NKDAVKRILE 100
QMDVIHRMCQ LYPETFMCVT NSSDILQAFR RGKVASLIGV EGGHLIDSSL 150
GVLRTLYHLG MRYLTLTHNC NTPWADNWLV DRGDDEAESH GLSPFGKRLL 200
NEMNRLGVMI DLSHVSVATM KDALQISRAP VIFSHSSAYS LCPHRRNVPD 250
DVLQLVKNTS SLVMVNFFSN FVSCSDSATL PQVADHLDHI KKVAGAGAVG 300
LGGDYDGVTM LPVGLEDVSK YPDLIAELLR RNWTETEVRG LLADNLIRVF 350
SEVELVSNNM QSPEEVPITL KELDGSCRTY YGYSQAHSIH LQTGALVASL 400
ASLLFRLHLL 410
Length:410
Mass (Da):45,722
Last modified:October 3, 2012 - v2
Checksum:i46A454B51CA57BB5
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti204 – 2041N → T in BAA02432. 1 Publication
Sequence conflicti358 – 3581N → S in BAA02432. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13139 mRNA. Translation: BAA02432.1.
AC163617 Genomic DNA. No translation available.
CH466525 Genomic DNA. Translation: EDL11712.1.
CCDSiCCDS22750.1.
PIRiS33757.
RefSeqiNP_031902.2. NM_007876.2.
UniGeneiMm.20388.

Genome annotation databases

EnsembliENSMUST00000019422; ENSMUSP00000019422; ENSMUSG00000019278.
GeneIDi13479.
KEGGimmu:13479.
UCSCiuc009nui.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13139 mRNA. Translation: BAA02432.1 .
AC163617 Genomic DNA. No translation available.
CH466525 Genomic DNA. Translation: EDL11712.1 .
CCDSi CCDS22750.1.
PIRi S33757.
RefSeqi NP_031902.2. NM_007876.2.
UniGenei Mm.20388.

3D structure databases

ProteinModelPortali P31428.
SMRi P31428. Positions 17-384.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P31428. 3 interactions.
MINTi MINT-4101665.
STRINGi 10090.ENSMUSP00000019422.

Protein family/group databases

MEROPSi M19.001.

PTM databases

PhosphoSitei P31428.

Proteomic databases

PaxDbi P31428.
PRIDEi P31428.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000019422 ; ENSMUSP00000019422 ; ENSMUSG00000019278 .
GeneIDi 13479.
KEGGi mmu:13479.
UCSCi uc009nui.2. mouse.

Organism-specific databases

CTDi 1800.
MGIi MGI:94917. Dpep1.

Phylogenomic databases

eggNOGi COG2355.
GeneTreei ENSGT00390000017920.
HOGENOMi HOG000072016.
HOVERGENi HBG002339.
InParanoidi P31428.
KOi K01273.
OMAi GMRYMTL.
OrthoDBi EOG7SJD4N.
TreeFami TF324523.

Enzyme and pathway databases

Reactomei REACT_215561. Synthesis of Leukotrienes (LT) and Eoxins (EX).

Miscellaneous databases

NextBioi 283967.
PROi P31428.
SOURCEi Search...

Gene expression databases

CleanExi MM_DPEP1.
MM_MBD1.
Genevestigatori P31428.

Family and domain databases

InterProi IPR000180. Dipep_AS.
IPR028536. Dpep1.
IPR008257. Renal_dipep_fam.
[Graphical view ]
PANTHERi PTHR10443. PTHR10443. 1 hit.
PTHR10443:SF17. PTHR10443:SF17. 1 hit.
Pfami PF01244. Peptidase_M19. 1 hit.
[Graphical view ]
PROSITEi PS00869. RENAL_DIPEPTIDASE_1. 1 hit.
PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purification and molecular cloning of mouse renal dipeptidase."
    Satoh S., Keida Y., Konta Y., Maeda M., Matsumoto Y., Niwa M., Kohsaka M.
    Biochim. Biophys. Acta 1163:234-242(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Identification of two additional members of the membrane-bound dipeptidase family."
    Habib G.M., Shi Z.-Z., Cuevas A.A., Lieberman M.W.
    FASEB J. 17:1313-1315(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.

Entry informationi

Entry nameiDPEP1_MOUSE
AccessioniPrimary (citable) accession number: P31428
Secondary accession number(s): G5E824
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 3, 2012
Last modified: September 3, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi