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Protein

Dipeptidase 1

Gene

Dpep1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity.

Catalytic activityi

Hydrolysis of dipeptides.PROSITE-ProRule annotation

Cofactori

Enzyme regulationi

Inhibited by L-penicillamine.1 Publication

Kineticsi

  1. KM=10 µM for leukotriene D41 Publication
  2. KM=0.45 mM for cystinyl-bis-glycine1 Publication
  3. KM=111 µM for beta-lactam1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi36Zinc 1; catalyticPROSITE-ProRule annotation1
    Metal bindingi38Zinc 1; catalyticPROSITE-ProRule annotation1
    Metal bindingi141Zinc 1; catalyticPROSITE-ProRule annotation1
    Metal bindingi141Zinc 2; catalyticPROSITE-ProRule annotation1
    Binding sitei168SubstratePROSITE-ProRule annotation1
    Metal bindingi214Zinc 2; catalyticPROSITE-ProRule annotation1
    Metal bindingi235Zinc 2; catalyticPROSITE-ProRule annotation1
    Binding sitei246SubstratePROSITE-ProRule annotation1
    Binding sitei304SubstratePROSITE-ProRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Dipeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiR-MMU-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
    R-MMU-5423646. Aflatoxin activation and detoxification.

    Protein family/group databases

    MEROPSiM19.001.

    Chemistry databases

    SwissLipidsiSLP:000001461.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dipeptidase 1 (EC:3.4.13.19)
    Alternative name(s):
    Membrane-bound dipeptidase 1
    Short name:
    MBD-1
    Microsomal dipeptidase
    Renal dipeptidase
    Gene namesi
    Name:Dpep1
    Synonyms:Mbd1, Rdp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 8

    Organism-specific databases

    MGIiMGI:94917. Dpep1.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 16By similarityAdd BLAST16
    ChainiPRO_000001865417 – 384Dipeptidase 1Add BLAST368
    PropeptideiPRO_0000018655385 – 410Removed in mature formBy similarityAdd BLAST26

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi87 ↔ 170PROSITE-ProRule annotation
    Glycosylationi121N-linked (GlcNAc...)Sequence analysis1
    Disulfide bondi242 ↔ 274PROSITE-ProRule annotation
    Glycosylationi258N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi332N-linked (GlcNAc...)By similarity1
    Disulfide bondi377InterchainPROSITE-ProRule annotation
    Lipidationi384GPI-anchor amidated serineBy similarity1

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    MaxQBiP31428.
    PaxDbiP31428.
    PeptideAtlasiP31428.
    PRIDEiP31428.

    PTM databases

    PhosphoSitePlusiP31428.

    Expressioni

    Gene expression databases

    BgeeiENSMUSG00000019278.
    CleanExiMM_DPEP1.
    MM_MBD1.
    GenevisibleiP31428. MM.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.

    Protein-protein interaction databases

    IntActiP31428. 3 interactors.
    MINTiMINT-4101665.
    STRINGi10090.ENSMUSP00000019422.

    Structurei

    3D structure databases

    ProteinModelPortaliP31428.
    SMRiP31428.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M19 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG4127. Eukaryota.
    COG2355. LUCA.
    GeneTreeiENSGT00390000017920.
    HOGENOMiHOG000072016.
    HOVERGENiHBG002339.
    InParanoidiP31428.
    KOiK01273.
    OMAiGMRYMTL.
    OrthoDBiEOG091G09CU.
    TreeFamiTF324523.

    Family and domain databases

    CDDicd01301. rDP_like. 1 hit.
    InterProiIPR000180. Dipep_AS.
    IPR028536. Dpep1.
    IPR032466. Metal_Hydrolase.
    IPR008257. Renal_dipep_fam.
    [Graphical view]
    PANTHERiPTHR10443. PTHR10443. 1 hit.
    PTHR10443:SF17. PTHR10443:SF17. 1 hit.
    PfamiPF01244. Peptidase_M19. 1 hit.
    [Graphical view]
    SUPFAMiSSF51556. SSF51556. 1 hit.
    PROSITEiPS00869. RENAL_DIPEPTIDASE_1. 1 hit.
    PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31428-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVIIWWFWSL LAICASDSFR DQAVAIMRTT PVIDGHNDLP WQLLNLFNNQ
    60 70 80 90 100
    LLRPDADLNK LAQTHTNIPK LKAGFVGGQF WSAYMPCDTQ NKDAVKRILE
    110 120 130 140 150
    QMDVIHRMCQ LYPETFMCVT NSSDILQAFR RGKVASLIGV EGGHLIDSSL
    160 170 180 190 200
    GVLRTLYHLG MRYLTLTHNC NTPWADNWLV DRGDDEAESH GLSPFGKRLL
    210 220 230 240 250
    NEMNRLGVMI DLSHVSVATM KDALQISRAP VIFSHSSAYS LCPHRRNVPD
    260 270 280 290 300
    DVLQLVKNTS SLVMVNFFSN FVSCSDSATL PQVADHLDHI KKVAGAGAVG
    310 320 330 340 350
    LGGDYDGVTM LPVGLEDVSK YPDLIAELLR RNWTETEVRG LLADNLIRVF
    360 370 380 390 400
    SEVELVSNNM QSPEEVPITL KELDGSCRTY YGYSQAHSIH LQTGALVASL
    410
    ASLLFRLHLL
    Length:410
    Mass (Da):45,722
    Last modified:October 3, 2012 - v2
    Checksum:i46A454B51CA57BB5
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti204N → T in BAA02432 (PubMed:8507661).Curated1
    Sequence conflicti358N → S in BAA02432 (PubMed:8507661).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D13139 mRNA. Translation: BAA02432.1.
    AC163617 Genomic DNA. No translation available.
    CH466525 Genomic DNA. Translation: EDL11712.1.
    CCDSiCCDS22750.1.
    PIRiS33757.
    RefSeqiNP_031902.2. NM_007876.2.
    XP_017168047.1. XM_017312558.1.
    UniGeneiMm.20388.

    Genome annotation databases

    EnsembliENSMUST00000019422; ENSMUSP00000019422; ENSMUSG00000019278.
    GeneIDi13479.
    KEGGimmu:13479.
    UCSCiuc009nui.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D13139 mRNA. Translation: BAA02432.1.
    AC163617 Genomic DNA. No translation available.
    CH466525 Genomic DNA. Translation: EDL11712.1.
    CCDSiCCDS22750.1.
    PIRiS33757.
    RefSeqiNP_031902.2. NM_007876.2.
    XP_017168047.1. XM_017312558.1.
    UniGeneiMm.20388.

    3D structure databases

    ProteinModelPortaliP31428.
    SMRiP31428.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiP31428. 3 interactors.
    MINTiMINT-4101665.
    STRINGi10090.ENSMUSP00000019422.

    Chemistry databases

    SwissLipidsiSLP:000001461.

    Protein family/group databases

    MEROPSiM19.001.

    PTM databases

    PhosphoSitePlusiP31428.

    Proteomic databases

    MaxQBiP31428.
    PaxDbiP31428.
    PeptideAtlasiP31428.
    PRIDEiP31428.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000019422; ENSMUSP00000019422; ENSMUSG00000019278.
    GeneIDi13479.
    KEGGimmu:13479.
    UCSCiuc009nui.2. mouse.

    Organism-specific databases

    CTDi1800.
    MGIiMGI:94917. Dpep1.

    Phylogenomic databases

    eggNOGiKOG4127. Eukaryota.
    COG2355. LUCA.
    GeneTreeiENSGT00390000017920.
    HOGENOMiHOG000072016.
    HOVERGENiHBG002339.
    InParanoidiP31428.
    KOiK01273.
    OMAiGMRYMTL.
    OrthoDBiEOG091G09CU.
    TreeFamiTF324523.

    Enzyme and pathway databases

    ReactomeiR-MMU-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
    R-MMU-5423646. Aflatoxin activation and detoxification.

    Miscellaneous databases

    PROiP31428.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000019278.
    CleanExiMM_DPEP1.
    MM_MBD1.
    GenevisibleiP31428. MM.

    Family and domain databases

    CDDicd01301. rDP_like. 1 hit.
    InterProiIPR000180. Dipep_AS.
    IPR028536. Dpep1.
    IPR032466. Metal_Hydrolase.
    IPR008257. Renal_dipep_fam.
    [Graphical view]
    PANTHERiPTHR10443. PTHR10443. 1 hit.
    PTHR10443:SF17. PTHR10443:SF17. 1 hit.
    PfamiPF01244. Peptidase_M19. 1 hit.
    [Graphical view]
    SUPFAMiSSF51556. SSF51556. 1 hit.
    PROSITEiPS00869. RENAL_DIPEPTIDASE_1. 1 hit.
    PS51365. RENAL_DIPEPTIDASE_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDPEP1_MOUSE
    AccessioniPrimary (citable) accession number: P31428
    Secondary accession number(s): G5E824
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: October 3, 2012
    Last modified: November 30, 2016
    This is version 144 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.