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P31423

- GRM4_RAT

UniProt

P31423 - GRM4_RAT

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Protein

Metabotropic glutamate receptor 4

Gene

Grm4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei159 – 1591GlutamateBy similarity
Binding sitei230 – 2301GlutamateBy similarity
Binding sitei312 – 3121GlutamateBy similarity
Binding sitei405 – 4051GlutamateBy similarity

GO - Molecular functioni

  1. calcium-dependent protein binding Source: RGD
  2. calmodulin binding Source: RGD
  3. glutamate receptor activity Source: UniProtKB
  4. G-protein coupled receptor activity Source: UniProtKB
  5. group III metabotropic glutamate receptor activity Source: RefGenome

GO - Biological processi

  1. activation of MAPK activity Source: Ensembl
  2. adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
  3. gamma-aminobutyric acid secretion Source: UniProtKB
  4. learning Source: Ensembl
  5. negative regulation of apoptotic process Source: UniProtKB
  6. negative regulation of cell proliferation Source: UniProtKB
  7. negative regulation of synaptic transmission Source: UniProtKB
  8. neuron projection development Source: UniProtKB
  9. regulation of neuron apoptotic process Source: UniProtKB
  10. regulation of synaptic transmission, glutamatergic Source: RefGenome
  11. synaptic transmission, GABAergic Source: UniProtKB
  12. synaptic transmission, glutamatergic Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 4
Short name:
mGluR4
Gene namesi
Name:Grm4
Synonyms:Gprc1d, Mglur4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2745. Grm4.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasmic vesicle Source: Ensembl
  2. dendritic shaft Source: UniProtKB
  3. integral component of plasma membrane Source: RefGenome
  4. presynaptic active zone membrane Source: UniProtKB
  5. terminal bouton Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 912880Metabotropic glutamate receptor 4PRO_0000012931Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi67 ↔ 109By similarity
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi249 ↔ 538By similarity
Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi372 ↔ 388By similarity
Disulfide bondi428 ↔ 435By similarity
Glycosylationi454 – 4541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi484 – 4841N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi520 ↔ 539By similarity
Disulfide bondi524 ↔ 542By similarity
Disulfide bondi545 ↔ 557By similarity
Disulfide bondi560 ↔ 573By similarity
Glycosylationi569 – 5691N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP31423.
PRIDEiP31423.

PTM databases

PhosphoSiteiP31423.

Expressioni

Tissue specificityi

Is widely distributed in the CNS. Predominant expression is seen in the granule cells of the cerebellum.

Gene expression databases

ExpressionAtlasiP31423. baseline and differential.
GenevestigatoriP31423.

Interactioni

Subunit structurei

Interacts with PICK1.1 Publication

Protein-protein interaction databases

BioGridi246582. 6 interactions.
DIPiDIP-41144N.
IntActiP31423. 4 interactions.
MINTiMINT-235145.

Structurei

3D structure databases

ProteinModelPortaliP31423.
SMRiP31423. Positions 38-508.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini33 – 587555ExtracellularSequence AnalysisAdd
BLAST
Topological domaini611 – 62414CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini646 – 65611ExtracellularSequence AnalysisAdd
BLAST
Topological domaini676 – 69924CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini721 – 75030ExtracellularSequence AnalysisAdd
BLAST
Topological domaini773 – 78513CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini809 – 82113ExtracellularSequence AnalysisAdd
BLAST
Topological domaini848 – 91265CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei588 – 61023Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei625 – 64521Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei657 – 67519Helical; Name=3Sequence AnalysisAdd
BLAST
Transmembranei700 – 72021Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei751 – 77222Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei786 – 80823Helical; Name=6Sequence AnalysisAdd
BLAST
Transmembranei822 – 84726Helical; Name=7Sequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni180 – 1823Glutamate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG295200.
HOVERGENiHBG107965.
InParanoidiP31423.
KOiK04607.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001786. GPCR_3_mtglu_rcpt_4.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01054. MTABOTROPC4R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31423-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGKGGWAWW WARLPLCLLL SLYAPWVPSS LGKPKGHPHM NSIRIDGDIT
60 70 80 90 100
LGGLFPVHGR GSEGKACGEL KKEKGIHRLE AMLFALDRIN NDPDLLPNIT
110 120 130 140 150
LGARILDTCS RDTHALEQSL TFVQALIEKD GTEVRCGSGG PPIITKPERV
160 170 180 190 200
VGVIGASGSS VSIMVANILR LFKIPQISYA STAPDLSDNS RYDFFSRVVP
210 220 230 240 250
SDTYQAQAMV DIVRALKWNY VSTLASEGSY GESGVEAFIQ KSRENGGVCI
260 270 280 290 300
AQSVKIPREP KTGEFDKIIK RLLETSNARG IIIFANEDDI RRVLEAARRA
310 320 330 340 350
NQTGHFFWMG SDSWGSKSAP VLRLEEVAEG AVTILPKRMS VRGFDRYFSS
360 370 380 390 400
RTLDNNRRNI WFAEFWEDNF HCKLSRHALK KGSHIKKCTN RERIGQDSAY
410 420 430 440 450
EQEGKVQFVI DAVYAMGHAL HAMHRDLCPG RVGLCPRMDP VDGTQLLKYI
460 470 480 490 500
RNVNFSGIAG NPVTFNENGD APGRYDIYQY QLRNGSAEYK VIGSWTDHLH
510 520 530 540 550
LRIERMQWPG SGQQLPRSIC SLPCQPGERK KTVKGMACCW HCEPCTGYQY
560 570 580 590 600
QVDRYTCKTC PYDMRPTENR TSCQPIPIVK LEWDSPWAVL PLFLAVVGIA
610 620 630 640 650
ATLFVVVTFV RYNDTPIVKA SGRELSYVLL AGIFLCYATT FLMIAEPDLG
660 670 680 690 700
TCSLRRIFLG LGMSISYAAL LTKTNRIYRI FEQGKRSVSA PRFISPASQL
710 720 730 740 750
AITFILISLQ LLGICVWFVV DPSHSVVDFQ DQRTLDPRFA RGVLKCDISD
760 770 780 790 800
LSLICLLGYS MLLMVTCTVY AIKTRGVPET FNEAKPIGFT MYTTCIVWLA
810 820 830 840 850
FIPIFFGTSQ SADKLYIQTT TLTVSVSLSA SVSLGMLYMP KVYIILFHPE
860 870 880 890 900
QNVPKRKRSL KAVVTAATMS NKFTQKGNFR PNGEAKSELC ENLETPALAT
910
KQTYVTYTNH AI
Length:912
Mass (Da):101,819
Last modified:July 1, 1993 - v1
Checksum:i336430EF19B4B577
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti124 – 1241Q → R in AAA93190. (PubMed:8338667)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M92077 mRNA. No translation available.
M90518 mRNA. Translation: AAA93190.1.
PIRiJH0563.
RefSeqiNP_073157.1. NM_022666.1.
UniGeneiRn.89046.

Genome annotation databases

GeneIDi24417.
KEGGirno:24417.
UCSCiRGD:2745. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M92077 mRNA. No translation available.
M90518 mRNA. Translation: AAA93190.1 .
PIRi JH0563.
RefSeqi NP_073157.1. NM_022666.1.
UniGenei Rn.89046.

3D structure databases

ProteinModelPortali P31423.
SMRi P31423. Positions 38-508.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246582. 6 interactions.
DIPi DIP-41144N.
IntActi P31423. 4 interactions.
MINTi MINT-235145.

Chemistry

BindingDBi P31423.
ChEMBLi CHEMBL2787.
GuidetoPHARMACOLOGYi 292.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P31423.

Proteomic databases

PaxDbi P31423.
PRIDEi P31423.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24417.
KEGGi rno:24417.
UCSCi RGD:2745. rat.

Organism-specific databases

CTDi 2914.
RGDi 2745. Grm4.

Phylogenomic databases

eggNOGi NOG295200.
HOVERGENi HBG107965.
InParanoidi P31423.
KOi K04607.

Miscellaneous databases

NextBioi 603263.

Gene expression databases

ExpressionAtlasi P31423. baseline and differential.
Genevestigatori P31423.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001786. GPCR_3_mtglu_rcpt_4.
IPR028082. Peripla_BP_I.
[Graphical view ]
Pfami PF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view ]
PRINTSi PR00248. GPCRMGR.
PR01054. MTABOTROPC4R.
PR00593. MTABOTROPICR.
SUPFAMi SSF53822. SSF53822. 1 hit.
PROSITEi PS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A family of metabotropic glutamate receptors."
    Tanabe Y., Masu M., Ishii T., Shigemoto R., Nakanishi S.
    Neuron 8:169-179(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The ligand-binding domain in metabotropic glutamate receptors is related to bacterial periplasmic binding proteins."
    O'Hara P.J., Sheppard P.O., Thoegersen H., Venezia D., Haldeman B.A., McGrane V., Houamed K.M., Thomsen C., Gilbert T.L., Mulvihill E.R.
    Neuron 11:41-52(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. Lubec G., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 347-357 AND 686-692, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  4. "Interaction of the C-terminal tail region of the metabotropic glutamate receptor 7 with the protein kinase C substrate PICK1."
    El Far O., Airas J., Wischmeyer E., Nehring R.B., Karschin A., Betz H.
    Eur. J. Neurosci. 12:4215-4221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PICK1.

Entry informationi

Entry nameiGRM4_RAT
AccessioniPrimary (citable) accession number: P31423
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 29, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3