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P31423 (GRM4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metabotropic glutamate receptor 4

Short name=mGluR4
Gene names
Name:Grm4
Synonyms:Gprc1d, Mglur4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length912 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

Subunit structure

Interacts with PICK1. Ref.4

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Is widely distributed in the CNS. Predominant expression is seen in the granule cells of the cerebellum.

Sequence similarities

Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of MAPK activity

Inferred from electronic annotation. Source: Ensembl

adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

gamma-aminobutyric acid secretion

Traceable author statement PubMed 11906782. Source: UniProtKB

learning

Inferred from electronic annotation. Source: Ensembl

negative regulation of apoptotic process

Traceable author statement PubMed 16178733. Source: UniProtKB

negative regulation of cell proliferation

Traceable author statement PubMed 16178733. Source: UniProtKB

negative regulation of synaptic transmission

Traceable author statement PubMed 16178733. Source: UniProtKB

neuron projection development

Traceable author statement PubMed 16178733. Source: UniProtKB

regulation of neuron apoptotic process

Non-traceable author statement PubMed 16178733. Source: UniProtKB

regulation of synaptic transmission, glutamatergic

Inferred from Biological aspect of Ancestor. Source: RefGenome

synaptic transmission, GABAergic

Traceable author statement PubMed 11906782. Source: UniProtKB

synaptic transmission, glutamatergic

Traceable author statement PubMed 11906782. Source: UniProtKB

   Cellular_componentcytoplasmic vesicle

Inferred from electronic annotation. Source: Ensembl

dendritic shaft

Inferred from direct assay PubMed 11906782. Source: UniProtKB

integral component of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

presynaptic active zone membrane

Inferred from direct assay PubMed 11906782. Source: UniProtKB

terminal bouton

Inferred from direct assay PubMed 11906782. Source: UniProtKB

   Molecular_functionG-protein coupled receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

calcium-dependent protein binding

Inferred from physical interaction PubMed 21855531. Source: RGD

calmodulin binding

Inferred from physical interaction PubMed 21855531. Source: RGD

glutamate receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

group III metabotropic glutamate receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 912880Metabotropic glutamate receptor 4
PRO_0000012931

Regions

Topological domain33 – 587555Extracellular Potential
Transmembrane588 – 61023Helical; Name=1; Potential
Topological domain611 – 62414Cytoplasmic Potential
Transmembrane625 – 64521Helical; Name=2; Potential
Topological domain646 – 65611Extracellular Potential
Transmembrane657 – 67519Helical; Name=3; Potential
Topological domain676 – 69924Cytoplasmic Potential
Transmembrane700 – 72021Helical; Name=4; Potential
Topological domain721 – 75030Extracellular Potential
Transmembrane751 – 77222Helical; Name=5; Potential
Topological domain773 – 78513Cytoplasmic Potential
Transmembrane786 – 80823Helical; Name=6; Potential
Topological domain809 – 82113Extracellular Potential
Transmembrane822 – 84726Helical; Name=7; Potential
Topological domain848 – 91265Cytoplasmic Potential
Region180 – 1823Glutamate binding By similarity

Sites

Binding site1591Glutamate By similarity
Binding site2301Glutamate By similarity
Binding site3121Glutamate By similarity
Binding site4051Glutamate By similarity

Amino acid modifications

Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation3011N-linked (GlcNAc...) Potential
Glycosylation4541N-linked (GlcNAc...) Potential
Glycosylation4841N-linked (GlcNAc...) Potential
Glycosylation5691N-linked (GlcNAc...) Potential
Disulfide bond67 ↔ 109 By similarity
Disulfide bond249 ↔ 538 By similarity
Disulfide bond372 ↔ 388 By similarity
Disulfide bond428 ↔ 435 By similarity
Disulfide bond520 ↔ 539 By similarity
Disulfide bond524 ↔ 542 By similarity
Disulfide bond545 ↔ 557 By similarity
Disulfide bond560 ↔ 573 By similarity

Experimental info

Sequence conflict1241Q → R in AAA93190. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P31423 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 336430EF19B4B577

FASTA912101,819
        10         20         30         40         50         60 
MSGKGGWAWW WARLPLCLLL SLYAPWVPSS LGKPKGHPHM NSIRIDGDIT LGGLFPVHGR 

        70         80         90        100        110        120 
GSEGKACGEL KKEKGIHRLE AMLFALDRIN NDPDLLPNIT LGARILDTCS RDTHALEQSL 

       130        140        150        160        170        180 
TFVQALIEKD GTEVRCGSGG PPIITKPERV VGVIGASGSS VSIMVANILR LFKIPQISYA 

       190        200        210        220        230        240 
STAPDLSDNS RYDFFSRVVP SDTYQAQAMV DIVRALKWNY VSTLASEGSY GESGVEAFIQ 

       250        260        270        280        290        300 
KSRENGGVCI AQSVKIPREP KTGEFDKIIK RLLETSNARG IIIFANEDDI RRVLEAARRA 

       310        320        330        340        350        360 
NQTGHFFWMG SDSWGSKSAP VLRLEEVAEG AVTILPKRMS VRGFDRYFSS RTLDNNRRNI 

       370        380        390        400        410        420 
WFAEFWEDNF HCKLSRHALK KGSHIKKCTN RERIGQDSAY EQEGKVQFVI DAVYAMGHAL 

       430        440        450        460        470        480 
HAMHRDLCPG RVGLCPRMDP VDGTQLLKYI RNVNFSGIAG NPVTFNENGD APGRYDIYQY 

       490        500        510        520        530        540 
QLRNGSAEYK VIGSWTDHLH LRIERMQWPG SGQQLPRSIC SLPCQPGERK KTVKGMACCW 

       550        560        570        580        590        600 
HCEPCTGYQY QVDRYTCKTC PYDMRPTENR TSCQPIPIVK LEWDSPWAVL PLFLAVVGIA 

       610        620        630        640        650        660 
ATLFVVVTFV RYNDTPIVKA SGRELSYVLL AGIFLCYATT FLMIAEPDLG TCSLRRIFLG 

       670        680        690        700        710        720 
LGMSISYAAL LTKTNRIYRI FEQGKRSVSA PRFISPASQL AITFILISLQ LLGICVWFVV 

       730        740        750        760        770        780 
DPSHSVVDFQ DQRTLDPRFA RGVLKCDISD LSLICLLGYS MLLMVTCTVY AIKTRGVPET 

       790        800        810        820        830        840 
FNEAKPIGFT MYTTCIVWLA FIPIFFGTSQ SADKLYIQTT TLTVSVSLSA SVSLGMLYMP 

       850        860        870        880        890        900 
KVYIILFHPE QNVPKRKRSL KAVVTAATMS NKFTQKGNFR PNGEAKSELC ENLETPALAT 

       910 
KQTYVTYTNH AI 

« Hide

References

[1]"A family of metabotropic glutamate receptors."
Tanabe Y., Masu M., Ishii T., Shigemoto R., Nakanishi S.
Neuron 8:169-179(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The ligand-binding domain in metabotropic glutamate receptors is related to bacterial periplasmic binding proteins."
O'Hara P.J., Sheppard P.O., Thoegersen H., Venezia D., Haldeman B.A., McGrane V., Houamed K.M., Thomsen C., Gilbert T.L., Mulvihill E.R.
Neuron 11:41-52(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]Lubec G., Kang S.U., Lubec S.
Submitted (SEP-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 347-357 AND 686-692, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain.
[4]"Interaction of the C-terminal tail region of the metabotropic glutamate receptor 7 with the protein kinase C substrate PICK1."
El Far O., Airas J., Wischmeyer E., Nehring R.B., Karschin A., Betz H.
Eur. J. Neurosci. 12:4215-4221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PICK1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M92077 mRNA. No translation available.
M90518 mRNA. Translation: AAA93190.1.
PIRJH0563.
RefSeqNP_073157.1. NM_022666.1.
UniGeneRn.89046.

3D structure databases

ProteinModelPortalP31423.
SMRP31423. Positions 38-508.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246582. 6 interactions.
DIPDIP-41144N.
IntActP31423. 4 interactions.
MINTMINT-235145.

Chemistry

BindingDBP31423.
ChEMBLCHEMBL2787.
GuidetoPHARMACOLOGY292.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP31423.

Proteomic databases

PaxDbP31423.
PRIDEP31423.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24417.
KEGGrno:24417.
UCSCRGD:2745. rat.

Organism-specific databases

CTD2914.
RGD2745. Grm4.

Phylogenomic databases

eggNOGNOG295200.
HOVERGENHBG107965.
KOK04607.

Gene expression databases

GenevestigatorP31423.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001786. GPCR_3_mtglu_rcpt_4.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSPR00248. GPCRMGR.
PR01054. MTABOTROPC4R.
PR00593. MTABOTROPICR.
SUPFAMSSF53822. SSF53822. 1 hit.
PROSITEPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio603263.

Entry information

Entry nameGRM4_RAT
AccessionPrimary (citable) accession number: P31423
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 11, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries