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P31423

- GRM4_RAT

UniProt

P31423 - GRM4_RAT

Protein

Metabotropic glutamate receptor 4

Gene

Grm4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei159 – 1591GlutamateBy similarity
    Binding sitei230 – 2301GlutamateBy similarity
    Binding sitei312 – 3121GlutamateBy similarity
    Binding sitei405 – 4051GlutamateBy similarity

    GO - Molecular functioni

    1. calcium-dependent protein binding Source: RGD
    2. calmodulin binding Source: RGD
    3. glutamate receptor activity Source: UniProtKB
    4. G-protein coupled receptor activity Source: UniProtKB
    5. group III metabotropic glutamate receptor activity Source: RefGenome

    GO - Biological processi

    1. activation of MAPK activity Source: Ensembl
    2. adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
    3. gamma-aminobutyric acid secretion Source: UniProtKB
    4. learning Source: Ensembl
    5. negative regulation of apoptotic process Source: UniProtKB
    6. negative regulation of cell proliferation Source: UniProtKB
    7. negative regulation of synaptic transmission Source: UniProtKB
    8. neuron projection development Source: UniProtKB
    9. regulation of neuron apoptotic process Source: UniProtKB
    10. regulation of synaptic transmission, glutamatergic Source: RefGenome
    11. synaptic transmission, GABAergic Source: UniProtKB
    12. synaptic transmission, glutamatergic Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metabotropic glutamate receptor 4
    Short name:
    mGluR4
    Gene namesi
    Name:Grm4
    Synonyms:Gprc1d, Mglur4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2745. Grm4.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic vesicle Source: Ensembl
    2. dendritic shaft Source: UniProtKB
    3. integral component of plasma membrane Source: RefGenome
    4. presynaptic active zone membrane Source: UniProtKB
    5. terminal bouton Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Sequence AnalysisAdd
    BLAST
    Chaini33 – 912880Metabotropic glutamate receptor 4PRO_0000012931Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi67 ↔ 109By similarity
    Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi249 ↔ 538By similarity
    Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi372 ↔ 388By similarity
    Disulfide bondi428 ↔ 435By similarity
    Glycosylationi454 – 4541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi484 – 4841N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi520 ↔ 539By similarity
    Disulfide bondi524 ↔ 542By similarity
    Disulfide bondi545 ↔ 557By similarity
    Disulfide bondi560 ↔ 573By similarity
    Glycosylationi569 – 5691N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP31423.
    PRIDEiP31423.

    PTM databases

    PhosphoSiteiP31423.

    Expressioni

    Tissue specificityi

    Is widely distributed in the CNS. Predominant expression is seen in the granule cells of the cerebellum.

    Gene expression databases

    GenevestigatoriP31423.

    Interactioni

    Subunit structurei

    Interacts with PICK1.1 Publication

    Protein-protein interaction databases

    BioGridi246582. 6 interactions.
    DIPiDIP-41144N.
    IntActiP31423. 4 interactions.
    MINTiMINT-235145.

    Structurei

    3D structure databases

    ProteinModelPortaliP31423.
    SMRiP31423. Positions 38-508.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini33 – 587555ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini611 – 62414CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini646 – 65611ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini676 – 69924CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini721 – 75030ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini773 – 78513CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini809 – 82113ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini848 – 91265CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei588 – 61023Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei625 – 64521Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei657 – 67519Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei700 – 72021Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei751 – 77222Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei786 – 80823Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei822 – 84726Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni180 – 1823Glutamate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG295200.
    HOVERGENiHBG107965.
    KOiK04607.

    Family and domain databases

    InterProiIPR001828. ANF_lig-bd_rcpt.
    IPR000337. GPCR_3.
    IPR011500. GPCR_3_9-Cys_dom.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR000162. GPCR_3_mtglu_rcpt.
    IPR001786. GPCR_3_mtglu_rcpt_4.
    IPR028082. Peripla_BP_I.
    [Graphical view]
    PfamiPF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    PF07562. NCD3G. 1 hit.
    [Graphical view]
    PRINTSiPR00248. GPCRMGR.
    PR01054. MTABOTROPC4R.
    PR00593. MTABOTROPICR.
    SUPFAMiSSF53822. SSF53822. 1 hit.
    PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
    PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
    PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31423-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGKGGWAWW WARLPLCLLL SLYAPWVPSS LGKPKGHPHM NSIRIDGDIT    50
    LGGLFPVHGR GSEGKACGEL KKEKGIHRLE AMLFALDRIN NDPDLLPNIT 100
    LGARILDTCS RDTHALEQSL TFVQALIEKD GTEVRCGSGG PPIITKPERV 150
    VGVIGASGSS VSIMVANILR LFKIPQISYA STAPDLSDNS RYDFFSRVVP 200
    SDTYQAQAMV DIVRALKWNY VSTLASEGSY GESGVEAFIQ KSRENGGVCI 250
    AQSVKIPREP KTGEFDKIIK RLLETSNARG IIIFANEDDI RRVLEAARRA 300
    NQTGHFFWMG SDSWGSKSAP VLRLEEVAEG AVTILPKRMS VRGFDRYFSS 350
    RTLDNNRRNI WFAEFWEDNF HCKLSRHALK KGSHIKKCTN RERIGQDSAY 400
    EQEGKVQFVI DAVYAMGHAL HAMHRDLCPG RVGLCPRMDP VDGTQLLKYI 450
    RNVNFSGIAG NPVTFNENGD APGRYDIYQY QLRNGSAEYK VIGSWTDHLH 500
    LRIERMQWPG SGQQLPRSIC SLPCQPGERK KTVKGMACCW HCEPCTGYQY 550
    QVDRYTCKTC PYDMRPTENR TSCQPIPIVK LEWDSPWAVL PLFLAVVGIA 600
    ATLFVVVTFV RYNDTPIVKA SGRELSYVLL AGIFLCYATT FLMIAEPDLG 650
    TCSLRRIFLG LGMSISYAAL LTKTNRIYRI FEQGKRSVSA PRFISPASQL 700
    AITFILISLQ LLGICVWFVV DPSHSVVDFQ DQRTLDPRFA RGVLKCDISD 750
    LSLICLLGYS MLLMVTCTVY AIKTRGVPET FNEAKPIGFT MYTTCIVWLA 800
    FIPIFFGTSQ SADKLYIQTT TLTVSVSLSA SVSLGMLYMP KVYIILFHPE 850
    QNVPKRKRSL KAVVTAATMS NKFTQKGNFR PNGEAKSELC ENLETPALAT 900
    KQTYVTYTNH AI 912
    Length:912
    Mass (Da):101,819
    Last modified:July 1, 1993 - v1
    Checksum:i336430EF19B4B577
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti124 – 1241Q → R in AAA93190. (PubMed:8338667)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92077 mRNA. No translation available.
    M90518 mRNA. Translation: AAA93190.1.
    PIRiJH0563.
    RefSeqiNP_073157.1. NM_022666.1.
    UniGeneiRn.89046.

    Genome annotation databases

    GeneIDi24417.
    KEGGirno:24417.
    UCSCiRGD:2745. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92077 mRNA. No translation available.
    M90518 mRNA. Translation: AAA93190.1 .
    PIRi JH0563.
    RefSeqi NP_073157.1. NM_022666.1.
    UniGenei Rn.89046.

    3D structure databases

    ProteinModelPortali P31423.
    SMRi P31423. Positions 38-508.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246582. 6 interactions.
    DIPi DIP-41144N.
    IntActi P31423. 4 interactions.
    MINTi MINT-235145.

    Chemistry

    BindingDBi P31423.
    ChEMBLi CHEMBL2787.
    GuidetoPHARMACOLOGYi 292.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P31423.

    Proteomic databases

    PaxDbi P31423.
    PRIDEi P31423.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24417.
    KEGGi rno:24417.
    UCSCi RGD:2745. rat.

    Organism-specific databases

    CTDi 2914.
    RGDi 2745. Grm4.

    Phylogenomic databases

    eggNOGi NOG295200.
    HOVERGENi HBG107965.
    KOi K04607.

    Miscellaneous databases

    NextBioi 603263.

    Gene expression databases

    Genevestigatori P31423.

    Family and domain databases

    InterProi IPR001828. ANF_lig-bd_rcpt.
    IPR000337. GPCR_3.
    IPR011500. GPCR_3_9-Cys_dom.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR000162. GPCR_3_mtglu_rcpt.
    IPR001786. GPCR_3_mtglu_rcpt_4.
    IPR028082. Peripla_BP_I.
    [Graphical view ]
    Pfami PF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    PF07562. NCD3G. 1 hit.
    [Graphical view ]
    PRINTSi PR00248. GPCRMGR.
    PR01054. MTABOTROPC4R.
    PR00593. MTABOTROPICR.
    SUPFAMi SSF53822. SSF53822. 1 hit.
    PROSITEi PS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
    PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
    PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A family of metabotropic glutamate receptors."
      Tanabe Y., Masu M., Ishii T., Shigemoto R., Nakanishi S.
      Neuron 8:169-179(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "The ligand-binding domain in metabotropic glutamate receptors is related to bacterial periplasmic binding proteins."
      O'Hara P.J., Sheppard P.O., Thoegersen H., Venezia D., Haldeman B.A., McGrane V., Houamed K.M., Thomsen C., Gilbert T.L., Mulvihill E.R.
      Neuron 11:41-52(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. Lubec G., Kang S.U., Lubec S.
      Submitted (SEP-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 347-357 AND 686-692, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    4. "Interaction of the C-terminal tail region of the metabotropic glutamate receptor 7 with the protein kinase C substrate PICK1."
      El Far O., Airas J., Wischmeyer E., Nehring R.B., Karschin A., Betz H.
      Eur. J. Neurosci. 12:4215-4221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PICK1.

    Entry informationi

    Entry nameiGRM4_RAT
    AccessioniPrimary (citable) accession number: P31423
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3