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Protein

Metabotropic glutamate receptor 4

Gene

Grm4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei159GlutamateBy similarity1
Binding sitei230GlutamateBy similarity1
Binding sitei312GlutamateBy similarity1
Binding sitei405GlutamateBy similarity1

GO - Molecular functioni

  • calcium-dependent protein binding Source: RGD
  • calmodulin binding Source: RGD
  • glutamate receptor activity Source: UniProtKB
  • G-protein coupled receptor activity Source: UniProtKB
  • group III metabotropic glutamate receptor activity Source: GO_Central

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
  • gamma-aminobutyric acid secretion Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of cell proliferation Source: UniProtKB
  • negative regulation of synaptic transmission Source: UniProtKB
  • neuron projection development Source: UniProtKB
  • regulation of neuron apoptotic process Source: UniProtKB
  • regulation of synaptic transmission, glutamatergic Source: GO_Central
  • synaptic transmission, GABAergic Source: UniProtKB
  • synaptic transmission, glutamatergic Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 4
Short name:
mGluR4
Gene namesi
Name:Grm4
Synonyms:Gprc1d, Mglur4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2745. Grm4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini33 – 587ExtracellularSequence analysisAdd BLAST555
Transmembranei588 – 610Helical; Name=1Sequence analysisAdd BLAST23
Topological domaini611 – 624CytoplasmicSequence analysisAdd BLAST14
Transmembranei625 – 645Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini646 – 656ExtracellularSequence analysisAdd BLAST11
Transmembranei657 – 675Helical; Name=3Sequence analysisAdd BLAST19
Topological domaini676 – 699CytoplasmicSequence analysisAdd BLAST24
Transmembranei700 – 720Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini721 – 750ExtracellularSequence analysisAdd BLAST30
Transmembranei751 – 772Helical; Name=5Sequence analysisAdd BLAST22
Topological domaini773 – 785CytoplasmicSequence analysisAdd BLAST13
Transmembranei786 – 808Helical; Name=6Sequence analysisAdd BLAST23
Topological domaini809 – 821ExtracellularSequence analysisAdd BLAST13
Transmembranei822 – 847Helical; Name=7Sequence analysisAdd BLAST26
Topological domaini848 – 912CytoplasmicSequence analysisAdd BLAST65

GO - Cellular componenti

  • dendritic shaft Source: UniProtKB
  • integral component of plasma membrane Source: GO_Central
  • presynaptic active zone membrane Source: UniProtKB
  • terminal bouton Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2787.
GuidetoPHARMACOLOGYi292.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000001293133 – 912Metabotropic glutamate receptor 4Add BLAST880

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi67 ↔ 109By similarity
Glycosylationi98N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi249 ↔ 538By similarity
Glycosylationi301N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi372 ↔ 388By similarity
Disulfide bondi428 ↔ 435By similarity
Glycosylationi454N-linked (GlcNAc...)Sequence analysis1
Glycosylationi484N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi520 ↔ 539By similarity
Disulfide bondi524 ↔ 542By similarity
Disulfide bondi545 ↔ 557By similarity
Disulfide bondi560 ↔ 573By similarity
Glycosylationi569N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP31423.
PRIDEiP31423.

PTM databases

iPTMnetiP31423.
PhosphoSitePlusiP31423.
SwissPalmiP31423.

Expressioni

Tissue specificityi

Is widely distributed in the CNS. Predominant expression is seen in the granule cells of the cerebellum.

Gene expression databases

BgeeiENSRNOG00000000487.

Interactioni

Subunit structurei

Interacts with PICK1.1 Publication

GO - Molecular functioni

  • calcium-dependent protein binding Source: RGD
  • calmodulin binding Source: RGD

Protein-protein interaction databases

BioGridi246582. 5 interactors.
DIPiDIP-41144N.
IntActiP31423. 4 interactors.
MINTiMINT-235145.
STRINGi10116.ENSRNOP00000060812.

Chemistry databases

BindingDBiP31423.

Structurei

3D structure databases

ProteinModelPortaliP31423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni180 – 182Glutamate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
HOVERGENiHBG107965.
InParanoidiP31423.
KOiK04607.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001786. GPCR_3_mtglu_rcpt_4.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01054. MTABOTROPC4R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGKGGWAWW WARLPLCLLL SLYAPWVPSS LGKPKGHPHM NSIRIDGDIT
60 70 80 90 100
LGGLFPVHGR GSEGKACGEL KKEKGIHRLE AMLFALDRIN NDPDLLPNIT
110 120 130 140 150
LGARILDTCS RDTHALEQSL TFVQALIEKD GTEVRCGSGG PPIITKPERV
160 170 180 190 200
VGVIGASGSS VSIMVANILR LFKIPQISYA STAPDLSDNS RYDFFSRVVP
210 220 230 240 250
SDTYQAQAMV DIVRALKWNY VSTLASEGSY GESGVEAFIQ KSRENGGVCI
260 270 280 290 300
AQSVKIPREP KTGEFDKIIK RLLETSNARG IIIFANEDDI RRVLEAARRA
310 320 330 340 350
NQTGHFFWMG SDSWGSKSAP VLRLEEVAEG AVTILPKRMS VRGFDRYFSS
360 370 380 390 400
RTLDNNRRNI WFAEFWEDNF HCKLSRHALK KGSHIKKCTN RERIGQDSAY
410 420 430 440 450
EQEGKVQFVI DAVYAMGHAL HAMHRDLCPG RVGLCPRMDP VDGTQLLKYI
460 470 480 490 500
RNVNFSGIAG NPVTFNENGD APGRYDIYQY QLRNGSAEYK VIGSWTDHLH
510 520 530 540 550
LRIERMQWPG SGQQLPRSIC SLPCQPGERK KTVKGMACCW HCEPCTGYQY
560 570 580 590 600
QVDRYTCKTC PYDMRPTENR TSCQPIPIVK LEWDSPWAVL PLFLAVVGIA
610 620 630 640 650
ATLFVVVTFV RYNDTPIVKA SGRELSYVLL AGIFLCYATT FLMIAEPDLG
660 670 680 690 700
TCSLRRIFLG LGMSISYAAL LTKTNRIYRI FEQGKRSVSA PRFISPASQL
710 720 730 740 750
AITFILISLQ LLGICVWFVV DPSHSVVDFQ DQRTLDPRFA RGVLKCDISD
760 770 780 790 800
LSLICLLGYS MLLMVTCTVY AIKTRGVPET FNEAKPIGFT MYTTCIVWLA
810 820 830 840 850
FIPIFFGTSQ SADKLYIQTT TLTVSVSLSA SVSLGMLYMP KVYIILFHPE
860 870 880 890 900
QNVPKRKRSL KAVVTAATMS NKFTQKGNFR PNGEAKSELC ENLETPALAT
910
KQTYVTYTNH AI
Length:912
Mass (Da):101,819
Last modified:July 1, 1993 - v1
Checksum:i336430EF19B4B577
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti124Q → R in AAA93190 (PubMed:8338667).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92077 mRNA. No translation available.
M90518 mRNA. Translation: AAA93190.1.
PIRiJH0563.
RefSeqiNP_073157.1. NM_022666.1.
UniGeneiRn.89046.

Genome annotation databases

GeneIDi24417.
KEGGirno:24417.
UCSCiRGD:2745. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92077 mRNA. No translation available.
M90518 mRNA. Translation: AAA93190.1.
PIRiJH0563.
RefSeqiNP_073157.1. NM_022666.1.
UniGeneiRn.89046.

3D structure databases

ProteinModelPortaliP31423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246582. 5 interactors.
DIPiDIP-41144N.
IntActiP31423. 4 interactors.
MINTiMINT-235145.
STRINGi10116.ENSRNOP00000060812.

Chemistry databases

BindingDBiP31423.
ChEMBLiCHEMBL2787.
GuidetoPHARMACOLOGYi292.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP31423.
PhosphoSitePlusiP31423.
SwissPalmiP31423.

Proteomic databases

PaxDbiP31423.
PRIDEiP31423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24417.
KEGGirno:24417.
UCSCiRGD:2745. rat.

Organism-specific databases

CTDi2914.
RGDi2745. Grm4.

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
HOVERGENiHBG107965.
InParanoidiP31423.
KOiK04607.

Miscellaneous databases

PROiP31423.

Gene expression databases

BgeeiENSRNOG00000000487.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001786. GPCR_3_mtglu_rcpt_4.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01054. MTABOTROPC4R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRM4_RAT
AccessioniPrimary (citable) accession number: P31423
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.