P31423 (GRM4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metabotropic glutamate receptor 4
Short name=mGluR4

Gene names

Name:	Grm4
Synonyms:	Gprc1d, Mglur4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	912 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity.
Subunit structure	Interacts with PICK1. Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Is widely distributed in the CNS. Predominant expression is seen in the granule cells of the cerebellum.
Sequence similarities	Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	activation of MAPK activity Inferred from electronic annotation. Source: Compara adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Inferred from electronic annotation. Source: Compara gamma-aminobutyric acid secretion Traceable author statement PubMed 11906782. Source: UniProtKB learning Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Traceable author statement PubMed 16178733. Source: UniProtKB negative regulation of cell proliferation Traceable author statement PubMed 16178733. Source: UniProtKB negative regulation of synaptic transmission Traceable author statement PubMed 16178733. Source: UniProtKB neuron projection development Traceable author statement PubMed 16178733. Source: UniProtKB regulation of neuron apoptotic process Non-traceable author statement PubMed 16178733. Source: UniProtKB synaptic transmission, GABAergic Traceable author statement PubMed 11906782. Source: UniProtKB synaptic transmission, glutamatergic Traceable author statement PubMed 11906782. Source: UniProtKB
Cellular_component	cytoplasmic vesicle Inferred from electronic annotation. Source: Compara dendritic shaft Inferred from direct assay PubMed 11906782. Source: UniProtKB integral to plasma membrane Inferred from electronic annotation. Source: Compara presynaptic active zone membrane Inferred from direct assay PubMed 11906782. Source: UniProtKB terminal bouton Inferred from direct assay PubMed 11906782. Source: UniProtKB
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 912	880	Metabotropic glutamate receptor 4		PRO_0000012931
Regions
	Topological domain	33 – 587	555	Extracellular Potential
	Transmembrane	588 – 610	23	Helical; Name=1; Potential
	Topological domain	611 – 624	14	Cytoplasmic Potential
	Transmembrane	625 – 645	21	Helical; Name=2; Potential
	Topological domain	646 – 656	11	Extracellular Potential
	Transmembrane	657 – 675	19	Helical; Name=3; Potential
	Topological domain	676 – 699	24	Cytoplasmic Potential
	Transmembrane	700 – 720	21	Helical; Name=4; Potential
	Topological domain	721 – 750	30	Extracellular Potential
	Transmembrane	751 – 772	22	Helical; Name=5; Potential
	Topological domain	773 – 785	13	Cytoplasmic Potential
	Transmembrane	786 – 808	23	Helical; Name=6; Potential
	Topological domain	809 – 821	13	Extracellular Potential
	Transmembrane	822 – 847	26	Helical; Name=7; Potential
	Topological domain	848 – 912	65	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	98	1	N-linked (GlcNAc...) Potential
	Glycosylation	301	1	N-linked (GlcNAc...) Potential
	Glycosylation	454	1	N-linked (GlcNAc...) Potential
	Glycosylation	484	1	N-linked (GlcNAc...) Potential
	Glycosylation	569	1	N-linked (GlcNAc...) Potential
	Disulfide bond	67 ↔ 109		By similarity
	Disulfide bond	249 ↔ 538		By similarity
	Disulfide bond	372 ↔ 388		By similarity
	Disulfide bond	428 ↔ 435		By similarity
	Disulfide bond	520 ↔ 539		By similarity
	Disulfide bond	524 ↔ 542		By similarity
	Disulfide bond	545 ↔ 557		By similarity
	Disulfide bond	560 ↔ 573		By similarity
Experimental info
	Sequence conflict	124	1	Q → R in AAA93190. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P31423 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 336430EF19B4B577	FASTA	912	101,819
	10 20 30 40 50 60 MSGKGGWAWW WARLPLCLLL SLYAPWVPSS LGKPKGHPHM NSIRIDGDIT LGGLFPVHGR 70 80 90 100 110 120 GSEGKACGEL KKEKGIHRLE AMLFALDRIN NDPDLLPNIT LGARILDTCS RDTHALEQSL 130 140 150 160 170 180 TFVQALIEKD GTEVRCGSGG PPIITKPERV VGVIGASGSS VSIMVANILR LFKIPQISYA 190 200 210 220 230 240 STAPDLSDNS RYDFFSRVVP SDTYQAQAMV DIVRALKWNY VSTLASEGSY GESGVEAFIQ 250 260 270 280 290 300 KSRENGGVCI AQSVKIPREP KTGEFDKIIK RLLETSNARG IIIFANEDDI RRVLEAARRA 310 320 330 340 350 360 NQTGHFFWMG SDSWGSKSAP VLRLEEVAEG AVTILPKRMS VRGFDRYFSS RTLDNNRRNI 370 380 390 400 410 420 WFAEFWEDNF HCKLSRHALK KGSHIKKCTN RERIGQDSAY EQEGKVQFVI DAVYAMGHAL 430 440 450 460 470 480 HAMHRDLCPG RVGLCPRMDP VDGTQLLKYI RNVNFSGIAG NPVTFNENGD APGRYDIYQY 490 500 510 520 530 540 QLRNGSAEYK VIGSWTDHLH LRIERMQWPG SGQQLPRSIC SLPCQPGERK KTVKGMACCW 550 560 570 580 590 600 HCEPCTGYQY QVDRYTCKTC PYDMRPTENR TSCQPIPIVK LEWDSPWAVL PLFLAVVGIA 610 620 630 640 650 660 ATLFVVVTFV RYNDTPIVKA SGRELSYVLL AGIFLCYATT FLMIAEPDLG TCSLRRIFLG 670 680 690 700 710 720 LGMSISYAAL LTKTNRIYRI FEQGKRSVSA PRFISPASQL AITFILISLQ LLGICVWFVV 730 740 750 760 770 780 DPSHSVVDFQ DQRTLDPRFA RGVLKCDISD LSLICLLGYS MLLMVTCTVY AIKTRGVPET 790 800 810 820 830 840 FNEAKPIGFT MYTTCIVWLA FIPIFFGTSQ SADKLYIQTT TLTVSVSLSA SVSLGMLYMP 850 860 870 880 890 900 KVYIILFHPE QNVPKRKRSL KAVVTAATMS NKFTQKGNFR PNGEAKSELC ENLETPALAT 910 KQTYVTYTNH AI

References

[1]	"A family of metabotropic glutamate receptors." Tanabe Y., Masu M., Ishii T., Shigemoto R., Nakanishi S. Neuron 8:169-179(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The ligand-binding domain in metabotropic glutamate receptors is related to bacterial periplasmic binding proteins." O'Hara P.J., Sheppard P.O., Thoegersen H., Venezia D., Haldeman B.A., McGrane V., Houamed K.M., Thomsen C., Gilbert T.L., Mulvihill E.R. Neuron 11:41-52(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	Lubec G., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 347-357 AND 686-692, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
[4]	"Interaction of the C-terminal tail region of the metabotropic glutamate receptor 7 with the protein kinase C substrate PICK1." El Far O., Airas J., Wischmeyer E., Nehring R.B., Karschin A., Betz H. Eur. J. Neurosci. 12:4215-4221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PICK1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M92077 mRNA. No translation available. M90518 mRNA. Translation: AAA93190.1.
IPI	IPI00327692.
PIR	JH0563.
RefSeq	NP_073157.1. NM_022666.1.
UniGene	Rn.89046.
3D structure databases
ProteinModelPortal	P31423.
SMR	P31423. Positions 38-508.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-41144N.
MINT	MINT-235145.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P31423.
Proteomic databases
PaxDb	P31423.
PRIDE	P31423.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24417.
KEGG	rno:24417.
UCSC	RGD:2745. rat.
Organism-specific databases
CTD	2914.
RGD	2745. Grm4.
Phylogenomic databases
eggNOG	NOG295200.
HOVERGEN	HBG107965.
KO	K04607.
Gene expression databases
Genevestigator	P31423.
Family and domain databases
InterPro	IPR001828. ANF_lig-bd_rcpt. IPR000337. GPCR_3. IPR011500. GPCR_3_9-Cys_dom. IPR017978. GPCR_3_C. IPR017979. GPCR_3_CS. IPR000162. GPCR_3_mtglu_rcpt. IPR001786. GPCR_3_mtglu_rcpt_4. [Graphical view]
Pfam	PF00003. 7tm_3. 1 hit. PF01094. ANF_receptor. 1 hit. PF07562. NCD3G. 1 hit. [Graphical view]
PRINTS	PR00248. GPCRMGR. PR01054. MTABOTROPC4R. PR00593. MTABOTROPICR.
PROSITE	PS00979. G_PROTEIN_RECEP_F3_1. 1 hit. PS00980. G_PROTEIN_RECEP_F3_2. 1 hit. PS00981. G_PROTEIN_RECEP_F3_3. 1 hit. PS50259. G_PROTEIN_RECEP_F3_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P31423.
ChEMBL	CHEMBL2787.
NextBio	603263.

Entry information

Entry name

GRM4_RAT

Accession

Primary (citable) accession number: P31423

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program