Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P31422

- GRM3_RAT

UniProt

P31422 - GRM3_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Metabotropic glutamate receptor 3

Gene

Grm3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681Glutamate
Binding sitei151 – 1511Glutamate
Binding sitei222 – 2221Glutamate
Binding sitei301 – 3011Glutamate
Binding sitei389 – 3891Glutamate

GO - Molecular functioni

  1. calcium channel regulator activity Source: Ensembl
  2. group II metabotropic glutamate receptor activity Source: RGD

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: GOC
  2. regulation of synaptic transmission Source: RGD
  3. regulation of synaptic transmission, glutamatergic Source: RefGenome
  4. sensory perception of pain Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_233455. G alpha (i) signalling events.
REACT_236177. Class C/3 (Metabotropic glutamate/pheromone receptors).

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 3
Short name:
mGluR3
Gene namesi
Name:Grm3
Synonyms:Gprc1c, Mglur3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi2744. Grm3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 576554ExtracellularSequence AnalysisAdd
BLAST
Transmembranei577 – 59923Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini600 – 61314CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei614 – 63421Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini635 – 64511ExtracellularSequence AnalysisAdd
BLAST
Transmembranei646 – 66419Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini665 – 68824CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei689 – 70921Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini710 – 73425ExtracellularSequence AnalysisAdd
BLAST
Transmembranei735 – 75622Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini757 – 76913CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei770 – 79223Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini793 – 80210ExtracellularSequence Analysis
Transmembranei803 – 82826Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini829 – 87951CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. astrocyte projection Source: RGD
  2. axon Source: Ensembl
  3. dendritic spine Source: Ensembl
  4. integral component of plasma membrane Source: RefGenome
  5. postsynaptic density Source: Ensembl
  6. postsynaptic membrane Source: Ensembl
  7. presynaptic membrane Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 879857Metabotropic glutamate receptor 3PRO_0000012929Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi57 ↔ 991 Publication
Glycosylationi209 – 2091N-linked (GlcNAc...)1 Publication
Disulfide bondi240 ↔ 5271 Publication
Glycosylationi292 – 2921N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi361 ↔ 3731 Publication
Disulfide bondi412 ↔ 4191 Publication
Glycosylationi414 – 4141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi509 ↔ 5281 Publication
Disulfide bondi513 ↔ 5311 Publication
Disulfide bondi534 ↔ 5461 Publication
Disulfide bondi549 ↔ 5621 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP31422.
PRIDEiP31422.

PTM databases

PhosphoSiteiP31422.

Expressioni

Tissue specificityi

Is widely distributed in the CNS. Predominant expression is seen in the neuronal cells of the cerebral cortex, dentate gyrus, and glial cells throughout brain regions.

Gene expression databases

GenevestigatoriP31422.

Interactioni

Subunit structurei

Interacts with GRASP.1 Publication

Protein-protein interaction databases

BioGridi246581. 1 interaction.
IntActiP31422. 4 interactions.
MINTiMINT-1773162.
STRINGi10116.ENSRNOP00000007572.

Structurei

Secondary structure

1
879
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 353Combined sources
Beta strandi38 – 458Combined sources
Beta strandi48 – 503Combined sources
Beta strandi56 – 605Combined sources
Turni62 – 654Combined sources
Helixi66 – 8116Combined sources
Beta strandi83 – 864Combined sources
Beta strandi91 – 977Combined sources
Helixi102 – 11413Combined sources
Beta strandi142 – 1476Combined sources
Helixi151 – 16111Combined sources
Helixi162 – 1643Combined sources
Beta strandi168 – 1725Combined sources
Helixi176 – 1794Combined sources
Turni181 – 1833Combined sources
Beta strandi187 – 1915Combined sources
Helixi194 – 20714Combined sources
Beta strandi212 – 2209Combined sources
Helixi223 – 23513Combined sources
Turni236 – 2383Combined sources
Beta strandi240 – 2478Combined sources
Helixi253 – 26412Combined sources
Beta strandi271 – 2755Combined sources
Helixi278 – 29013Combined sources
Beta strandi296 – 2994Combined sources
Turni301 – 3055Combined sources
Helixi307 – 3093Combined sources
Turni310 – 3123Combined sources
Helixi314 – 3174Combined sources
Beta strandi321 – 3255Combined sources
Helixi331 – 3388Combined sources
Turni342 – 3443Combined sources
Helixi351 – 3588Combined sources
Beta strandi363 – 3686Combined sources
Turni381 – 3833Combined sources
Helixi390 – 41122Combined sources
Helixi420 – 4223Combined sources
Helixi427 – 4348Combined sources
Beta strandi438 – 4403Combined sources
Beta strandi443 – 4453Combined sources
Beta strandi448 – 4503Combined sources
Beta strandi453 – 4553Combined sources
Beta strandi458 – 4603Combined sources
Beta strandi466 – 4727Combined sources
Beta strandi474 – 4774Combined sources
Beta strandi479 – 49113Combined sources
Helixi494 – 4963Combined sources
Helixi500 – 5023Combined sources
Turni515 – 5173Combined sources
Beta strandi518 – 5214Combined sources
Beta strandi524 – 5274Combined sources
Beta strandi530 – 5334Combined sources
Beta strandi538 – 5425Combined sources
Beta strandi545 – 5484Combined sources
Beta strandi553 – 5553Combined sources
Beta strandi559 – 5646Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E4UX-ray2.35A/B25-575[»]
2E4VX-ray2.40A/B25-575[»]
2E4WX-ray2.40A/B25-575[»]
2E4XX-ray2.75A/B25-575[»]
2E4YX-ray3.40A/B25-575[»]
ProteinModelPortaliP31422.
SMRiP31422. Positions 28-567.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31422.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni172 – 1743Glutamate binding

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG295200.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218635.
HOVERGENiHBG107965.
InParanoidiP31422.
KOiK04605.
OMAiCTCIVVT.
OrthoDBiEOG7Z0JXG.
PhylomeDBiP31422.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001234. GPCR_3_mtglu_rcpt_3.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01053. MTABOTROPC3R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31422-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKMLTRLQIL MLALFSKGFL LSLGDHNFMR REIKIEGDLV LGGLFPINEK
60 70 80 90 100
GTGTEECGRI NEDRGIQRLE AMLFAIDEIN KDNYLLPGVK LGVHILDTCS
110 120 130 140 150
RDTYALEQSL EFVRASLTKV DEAEYMCPDG SYAIQENIPL LIAGVIGGSY
160 170 180 190 200
SSVSIQVANL LRLFQIPQIS YASTSAKLSD KSRYDYFART VPPDFYQAKA
210 220 230 240 250
MAEILRFFNW TYVSTVASEG DYGETGIEAF EQEARLRNIC IATAEKVGRS
260 270 280 290 300
NIRKSYDSVI RELLQKPNAR VVVLFMRSDD SRELIAAANR VNASFTWVAS
310 320 330 340 350
DGWGAQESIV KGSEHVAYGA ITLELASHPV RQFDRYFQSL NPYNNHRNPW
360 370 380 390 400
FRDFWEQKFQ CSLQNKRNHR QVCDKHLAID SSNYEQESKI MFVVNAVYAM
410 420 430 440 450
AHALHKMQRT LCPNTTKLCD AMKILDGKKL YKEYLLKINF TAPFNPNKGA
460 470 480 490 500
DSIVKFDTFG DGMGRYNVFN LQQTGGKYSY LKVGHWAETL SLDVDSIHWS
510 520 530 540 550
RNSVPTSQCS DPCAPNEMKN MQPGDVCCWI CIPCEPYEYL VDEFTCMDCG
560 570 580 590 600
PGQWPTADLS GCYNLPEDYI KWEDAWAIGP VTIACLGFLC TCIVITVFIK
610 620 630 640 650
HNNTPLVKAS GRELCYILLF GVSLSYCMTF FFIAKPSPVI CALRRLGLGT
660 670 680 690 700
SFAICYSALL TKTNCIARIF DGVKNGAQRP KFISPSSQVF ICLGLILVQI
710 720 730 740 750
VMVSVWLILE TPGTRRYTLP EKRETVILKC NVKDSSMLIS LTYDVVLVIL
760 770 780 790 800
CTVYAFKTRK CPENFNEAKF IGFTMYTTCI IWLAFLPIFY VTSSDYRVQT
810 820 830 840 850
TTMCISVSLS GFVVLGCLFA PKVHIVLFQP QKNVVTHRLH LNRFSVSGTA
860 870
TTYSQSSAST YVPTVCNGRE VLDSTTSSL
Length:879
Mass (Da):98,960
Last modified:July 1, 1993 - v1
Checksum:i3E5965EDD5E6DEED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92076 mRNA. No translation available.
PIRiJH0562.
RefSeqiNP_001099182.1. NM_001105712.1.
XP_008760905.1. XM_008762683.1.
UniGeneiRn.41715.

Genome annotation databases

EnsembliENSRNOT00000007572; ENSRNOP00000007572; ENSRNOG00000005519.
GeneIDi24416.
KEGGirno:24416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92076 mRNA. No translation available.
PIRi JH0562.
RefSeqi NP_001099182.1. NM_001105712.1.
XP_008760905.1. XM_008762683.1.
UniGenei Rn.41715.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2E4U X-ray 2.35 A/B 25-575 [» ]
2E4V X-ray 2.40 A/B 25-575 [» ]
2E4W X-ray 2.40 A/B 25-575 [» ]
2E4X X-ray 2.75 A/B 25-575 [» ]
2E4Y X-ray 3.40 A/B 25-575 [» ]
ProteinModelPortali P31422.
SMRi P31422. Positions 28-567.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246581. 1 interaction.
IntActi P31422. 4 interactions.
MINTi MINT-1773162.
STRINGi 10116.ENSRNOP00000007572.

Chemistry

BindingDBi P31422.
ChEMBLi CHEMBL3067.
GuidetoPHARMACOLOGYi 291.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P31422.

Proteomic databases

PaxDbi P31422.
PRIDEi P31422.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000007572 ; ENSRNOP00000007572 ; ENSRNOG00000005519 .
GeneIDi 24416.
KEGGi rno:24416.

Organism-specific databases

CTDi 2913.
RGDi 2744. Grm3.

Phylogenomic databases

eggNOGi NOG295200.
GeneTreei ENSGT00760000118884.
HOGENOMi HOG000218635.
HOVERGENi HBG107965.
InParanoidi P31422.
KOi K04605.
OMAi CTCIVVT.
OrthoDBi EOG7Z0JXG.
PhylomeDBi P31422.

Enzyme and pathway databases

Reactomei REACT_233455. G alpha (i) signalling events.
REACT_236177. Class C/3 (Metabotropic glutamate/pheromone receptors).

Miscellaneous databases

EvolutionaryTracei P31422.
NextBioi 603257.

Gene expression databases

Genevestigatori P31422.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001234. GPCR_3_mtglu_rcpt_3.
IPR028082. Peripla_BP_I.
[Graphical view ]
Pfami PF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view ]
PRINTSi PR00248. GPCRMGR.
PR01053. MTABOTROPC3R.
PR00593. MTABOTROPICR.
SUPFAMi SSF53822. SSF53822. 1 hit.
PROSITEi PS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A family of metabotropic glutamate receptors."
    Tanabe Y., Masu M., Ishii T., Shigemoto R., Nakanishi S.
    Neuron 8:169-179(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins."
    Kitano J., Kimura K., Yamazaki Y., Soda T., Shigemoto R., Nakajima Y., Nakanishi S.
    J. Neurosci. 22:1280-1289(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRASP.
  3. "Structures of the extracellular regions of the group II/III metabotropic glutamate receptors."
    Muto T., Tsuchiya D., Morikawa K., Jingami H.
    Proc. Natl. Acad. Sci. U.S.A. 104:3759-3764(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 25-575 IN COMPLEXES WITH GLUTAMATE, GLYCOSYLATION AT ASN-209, DISULFIDE BONDS.

Entry informationi

Entry nameiGRM3_RAT
AccessioniPrimary (citable) accession number: P31422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 26, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3