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P31422

- GRM3_RAT

UniProt

P31422 - GRM3_RAT

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Protein
Metabotropic glutamate receptor 3
Gene
Grm3, Gprc1c, Mglur3
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681Glutamate
Binding sitei151 – 1511Glutamate
Binding sitei222 – 2221Glutamate
Binding sitei301 – 3011Glutamate
Binding sitei389 – 3891Glutamate

GO - Molecular functioni

  1. calcium channel regulator activity Source: Ensembl
  2. group II metabotropic glutamate receptor activity Source: RGD

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: GOC
  2. regulation of synaptic transmission Source: RGD
  3. regulation of synaptic transmission, glutamatergic Source: RefGenome
  4. sensory perception of pain Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 3
Short name:
mGluR3
Gene namesi
Name:Grm3
Synonyms:Gprc1c, Mglur3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi2744. Grm3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 576554Extracellular Reviewed prediction
Add
BLAST
Transmembranei577 – 59923Helical; Name=1; Reviewed prediction
Add
BLAST
Topological domaini600 – 61314Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei614 – 63421Helical; Name=2; Reviewed prediction
Add
BLAST
Topological domaini635 – 64511Extracellular Reviewed prediction
Add
BLAST
Transmembranei646 – 66419Helical; Name=3; Reviewed prediction
Add
BLAST
Topological domaini665 – 68824Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei689 – 70921Helical; Name=4; Reviewed prediction
Add
BLAST
Topological domaini710 – 73425Extracellular Reviewed prediction
Add
BLAST
Transmembranei735 – 75622Helical; Name=5; Reviewed prediction
Add
BLAST
Topological domaini757 – 76913Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei770 – 79223Helical; Name=6; Reviewed prediction
Add
BLAST
Topological domaini793 – 80210Extracellular Reviewed prediction
Transmembranei803 – 82826Helical; Name=7; Reviewed prediction
Add
BLAST
Topological domaini829 – 87951Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. astrocyte projection Source: RGD
  2. axon Source: Ensembl
  3. dendritic spine Source: Ensembl
  4. integral component of plasma membrane Source: RefGenome
  5. postsynaptic density Source: Ensembl
  6. postsynaptic membrane Source: Ensembl
  7. presynaptic membrane Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Chaini23 – 879857Metabotropic glutamate receptor 3
PRO_0000012929Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi57 ↔ 991 Publication
Glycosylationi209 – 2091N-linked (GlcNAc...)1 Publication
Disulfide bondi240 ↔ 5271 Publication
Glycosylationi292 – 2921N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi361 ↔ 3731 Publication
Disulfide bondi412 ↔ 4191 Publication
Glycosylationi414 – 4141N-linked (GlcNAc...) Reviewed prediction
Glycosylationi439 – 4391N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi509 ↔ 5281 Publication
Disulfide bondi513 ↔ 5311 Publication
Disulfide bondi534 ↔ 5461 Publication
Disulfide bondi549 ↔ 5621 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP31422.
PRIDEiP31422.

PTM databases

PhosphoSiteiP31422.

Expressioni

Tissue specificityi

Is widely distributed in the CNS. Predominant expression is seen in the neuronal cells of the cerebral cortex, dentate gyrus, and glial cells throughout brain regions.

Gene expression databases

ArrayExpressiP31422.
GenevestigatoriP31422.

Interactioni

Subunit structurei

Interacts with GRASP.1 Publication

Protein-protein interaction databases

BioGridi246581. 1 interaction.
IntActiP31422. 4 interactions.
MINTiMINT-1773162.
STRINGi10116.ENSRNOP00000007572.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 353
Beta strandi38 – 458
Beta strandi48 – 503
Beta strandi56 – 605
Turni62 – 654
Helixi66 – 8116
Beta strandi83 – 864
Beta strandi91 – 977
Helixi102 – 11413
Beta strandi142 – 1476
Helixi151 – 16111
Helixi162 – 1643
Beta strandi168 – 1725
Helixi176 – 1794
Turni181 – 1833
Beta strandi187 – 1915
Helixi194 – 20714
Beta strandi212 – 2209
Helixi223 – 23513
Turni236 – 2383
Beta strandi240 – 2478
Helixi253 – 26412
Beta strandi271 – 2755
Helixi278 – 29013
Beta strandi296 – 2994
Turni301 – 3055
Helixi307 – 3093
Turni310 – 3123
Helixi314 – 3174
Beta strandi321 – 3255
Helixi331 – 3388
Turni342 – 3443
Helixi351 – 3588
Beta strandi363 – 3686
Turni381 – 3833
Helixi390 – 41122
Helixi420 – 4223
Helixi427 – 4348
Beta strandi438 – 4403
Beta strandi443 – 4453
Beta strandi448 – 4503
Beta strandi453 – 4553
Beta strandi458 – 4603
Beta strandi466 – 4727
Beta strandi474 – 4774
Beta strandi479 – 49113
Helixi494 – 4963
Helixi500 – 5023
Turni515 – 5173
Beta strandi518 – 5214
Beta strandi524 – 5274
Beta strandi530 – 5334
Beta strandi538 – 5425
Beta strandi545 – 5484
Beta strandi553 – 5553
Beta strandi559 – 5646

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E4UX-ray2.35A/B25-575[»]
2E4VX-ray2.40A/B25-575[»]
2E4WX-ray2.40A/B25-575[»]
2E4XX-ray2.75A/B25-575[»]
2E4YX-ray3.40A/B25-575[»]
ProteinModelPortaliP31422.
SMRiP31422. Positions 28-567.

Miscellaneous databases

EvolutionaryTraceiP31422.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni172 – 1743Glutamate binding

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG295200.
GeneTreeiENSGT00750000117490.
HOGENOMiHOG000218635.
HOVERGENiHBG107965.
InParanoidiP31422.
KOiK04605.
OMAiCTCIVVT.
OrthoDBiEOG7Z0JXG.
PhylomeDBiP31422.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001234. GPCR_3_mtglu_rcpt_3.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01053. MTABOTROPC3R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31422-1 [UniParc]FASTAAdd to Basket

« Hide

MKMLTRLQIL MLALFSKGFL LSLGDHNFMR REIKIEGDLV LGGLFPINEK    50
GTGTEECGRI NEDRGIQRLE AMLFAIDEIN KDNYLLPGVK LGVHILDTCS 100
RDTYALEQSL EFVRASLTKV DEAEYMCPDG SYAIQENIPL LIAGVIGGSY 150
SSVSIQVANL LRLFQIPQIS YASTSAKLSD KSRYDYFART VPPDFYQAKA 200
MAEILRFFNW TYVSTVASEG DYGETGIEAF EQEARLRNIC IATAEKVGRS 250
NIRKSYDSVI RELLQKPNAR VVVLFMRSDD SRELIAAANR VNASFTWVAS 300
DGWGAQESIV KGSEHVAYGA ITLELASHPV RQFDRYFQSL NPYNNHRNPW 350
FRDFWEQKFQ CSLQNKRNHR QVCDKHLAID SSNYEQESKI MFVVNAVYAM 400
AHALHKMQRT LCPNTTKLCD AMKILDGKKL YKEYLLKINF TAPFNPNKGA 450
DSIVKFDTFG DGMGRYNVFN LQQTGGKYSY LKVGHWAETL SLDVDSIHWS 500
RNSVPTSQCS DPCAPNEMKN MQPGDVCCWI CIPCEPYEYL VDEFTCMDCG 550
PGQWPTADLS GCYNLPEDYI KWEDAWAIGP VTIACLGFLC TCIVITVFIK 600
HNNTPLVKAS GRELCYILLF GVSLSYCMTF FFIAKPSPVI CALRRLGLGT 650
SFAICYSALL TKTNCIARIF DGVKNGAQRP KFISPSSQVF ICLGLILVQI 700
VMVSVWLILE TPGTRRYTLP EKRETVILKC NVKDSSMLIS LTYDVVLVIL 750
CTVYAFKTRK CPENFNEAKF IGFTMYTTCI IWLAFLPIFY VTSSDYRVQT 800
TTMCISVSLS GFVVLGCLFA PKVHIVLFQP QKNVVTHRLH LNRFSVSGTA 850
TTYSQSSAST YVPTVCNGRE VLDSTTSSL 879
Length:879
Mass (Da):98,960
Last modified:July 1, 1993 - v1
Checksum:i3E5965EDD5E6DEED
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M92076 mRNA. No translation available.
PIRiJH0562.
RefSeqiNP_001099182.1. NM_001105712.1.
UniGeneiRn.41715.

Genome annotation databases

EnsembliENSRNOT00000007572; ENSRNOP00000007572; ENSRNOG00000005519.
GeneIDi24416.
KEGGirno:24416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M92076 mRNA. No translation available.
PIRi JH0562.
RefSeqi NP_001099182.1. NM_001105712.1.
UniGenei Rn.41715.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2E4U X-ray 2.35 A/B 25-575 [» ]
2E4V X-ray 2.40 A/B 25-575 [» ]
2E4W X-ray 2.40 A/B 25-575 [» ]
2E4X X-ray 2.75 A/B 25-575 [» ]
2E4Y X-ray 3.40 A/B 25-575 [» ]
ProteinModelPortali P31422.
SMRi P31422. Positions 28-567.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246581. 1 interaction.
IntActi P31422. 4 interactions.
MINTi MINT-1773162.
STRINGi 10116.ENSRNOP00000007572.

Chemistry

BindingDBi P31422.
ChEMBLi CHEMBL3067.
GuidetoPHARMACOLOGYi 291.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P31422.

Proteomic databases

PaxDbi P31422.
PRIDEi P31422.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000007572 ; ENSRNOP00000007572 ; ENSRNOG00000005519 .
GeneIDi 24416.
KEGGi rno:24416.

Organism-specific databases

CTDi 2913.
RGDi 2744. Grm3.

Phylogenomic databases

eggNOGi NOG295200.
GeneTreei ENSGT00750000117490.
HOGENOMi HOG000218635.
HOVERGENi HBG107965.
InParanoidi P31422.
KOi K04605.
OMAi CTCIVVT.
OrthoDBi EOG7Z0JXG.
PhylomeDBi P31422.

Miscellaneous databases

EvolutionaryTracei P31422.
NextBioi 603257.

Gene expression databases

ArrayExpressi P31422.
Genevestigatori P31422.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001234. GPCR_3_mtglu_rcpt_3.
IPR028082. Peripla_BP_I.
[Graphical view ]
Pfami PF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view ]
PRINTSi PR00248. GPCRMGR.
PR01053. MTABOTROPC3R.
PR00593. MTABOTROPICR.
SUPFAMi SSF53822. SSF53822. 1 hit.
PROSITEi PS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A family of metabotropic glutamate receptors."
    Tanabe Y., Masu M., Ishii T., Shigemoto R., Nakanishi S.
    Neuron 8:169-179(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins."
    Kitano J., Kimura K., Yamazaki Y., Soda T., Shigemoto R., Nakajima Y., Nakanishi S.
    J. Neurosci. 22:1280-1289(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRASP.
  3. "Structures of the extracellular regions of the group II/III metabotropic glutamate receptors."
    Muto T., Tsuchiya D., Morikawa K., Jingami H.
    Proc. Natl. Acad. Sci. U.S.A. 104:3759-3764(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 25-575 IN COMPLEXES WITH GLUTAMATE, GLYCOSYLATION AT ASN-209, DISULFIDE BONDS.

Entry informationi

Entry nameiGRM3_RAT
AccessioniPrimary (citable) accession number: P31422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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