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Protein

Metabotropic glutamate receptor 3

Gene

Grm3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68Glutamate1
Binding sitei151Glutamate1
Binding sitei222Glutamate1
Binding sitei301Glutamate1
Binding sitei389Glutamate1

GO - Molecular functioni

  • group II metabotropic glutamate receptor activity Source: RGD

GO - Biological processi

  • modulation of synaptic transmission Source: RGD
  • regulation of synaptic transmission, glutamatergic Source: GO_Central
  • sensory perception of pain Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-418594. G alpha (i) signalling events.
R-RNO-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 3
Short name:
mGluR3
Gene namesi
Name:Grm3
Synonyms:Gprc1c, Mglur3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2744. Grm3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 576ExtracellularSequence analysisAdd BLAST554
Transmembranei577 – 599Helical; Name=1Sequence analysisAdd BLAST23
Topological domaini600 – 613CytoplasmicSequence analysisAdd BLAST14
Transmembranei614 – 634Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini635 – 645ExtracellularSequence analysisAdd BLAST11
Transmembranei646 – 664Helical; Name=3Sequence analysisAdd BLAST19
Topological domaini665 – 688CytoplasmicSequence analysisAdd BLAST24
Transmembranei689 – 709Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini710 – 734ExtracellularSequence analysisAdd BLAST25
Transmembranei735 – 756Helical; Name=5Sequence analysisAdd BLAST22
Topological domaini757 – 769CytoplasmicSequence analysisAdd BLAST13
Transmembranei770 – 792Helical; Name=6Sequence analysisAdd BLAST23
Topological domaini793 – 802ExtracellularSequence analysis10
Transmembranei803 – 828Helical; Name=7Sequence analysisAdd BLAST26
Topological domaini829 – 879CytoplasmicSequence analysisAdd BLAST51

GO - Cellular componenti

  • astrocyte projection Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • presynaptic membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3067.
GuidetoPHARMACOLOGYi291.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000001292923 – 879Metabotropic glutamate receptor 3Add BLAST857

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi57 ↔ 991 Publication
Glycosylationi209N-linked (GlcNAc...)1 Publication1
Disulfide bondi240 ↔ 5271 Publication
Glycosylationi292N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi361 ↔ 3731 Publication
Disulfide bondi412 ↔ 4191 Publication
Glycosylationi414N-linked (GlcNAc...)Sequence analysis1
Glycosylationi439N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi509 ↔ 5281 Publication
Disulfide bondi513 ↔ 5311 Publication
Disulfide bondi534 ↔ 5461 Publication
Disulfide bondi549 ↔ 5621 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP31422.
PRIDEiP31422.

PTM databases

iPTMnetiP31422.
PhosphoSitePlusiP31422.
UniCarbKBiP31422.

Expressioni

Tissue specificityi

Is widely distributed in the CNS. Predominant expression is seen in the neuronal cells of the cerebral cortex, dentate gyrus, and glial cells throughout brain regions.

Gene expression databases

BgeeiENSRNOG00000005519.
ExpressionAtlasiP31422. baseline and differential.
GenevisibleiP31422. RN.

Interactioni

Subunit structurei

Interacts with GRASP.1 Publication

Protein-protein interaction databases

BioGridi246581. 1 interactor.
DIPiDIP-41143N.
IntActiP31422. 4 interactors.
MINTiMINT-1773162.
STRINGi10116.ENSRNOP00000007572.

Chemistry databases

BindingDBiP31422.

Structurei

Secondary structure

1879
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 35Combined sources3
Beta strandi38 – 45Combined sources8
Beta strandi48 – 50Combined sources3
Beta strandi56 – 60Combined sources5
Turni62 – 65Combined sources4
Helixi66 – 81Combined sources16
Beta strandi83 – 86Combined sources4
Beta strandi91 – 97Combined sources7
Helixi102 – 114Combined sources13
Beta strandi142 – 147Combined sources6
Helixi151 – 161Combined sources11
Helixi162 – 164Combined sources3
Beta strandi168 – 172Combined sources5
Helixi176 – 179Combined sources4
Turni181 – 183Combined sources3
Beta strandi187 – 191Combined sources5
Helixi194 – 207Combined sources14
Beta strandi212 – 220Combined sources9
Helixi223 – 235Combined sources13
Turni236 – 238Combined sources3
Beta strandi240 – 247Combined sources8
Helixi253 – 264Combined sources12
Beta strandi271 – 275Combined sources5
Helixi278 – 290Combined sources13
Beta strandi296 – 299Combined sources4
Turni301 – 305Combined sources5
Helixi307 – 309Combined sources3
Turni310 – 312Combined sources3
Helixi314 – 317Combined sources4
Beta strandi321 – 325Combined sources5
Helixi331 – 338Combined sources8
Turni342 – 344Combined sources3
Helixi351 – 358Combined sources8
Beta strandi363 – 368Combined sources6
Turni381 – 383Combined sources3
Helixi390 – 411Combined sources22
Helixi420 – 422Combined sources3
Helixi427 – 434Combined sources8
Beta strandi438 – 440Combined sources3
Beta strandi443 – 445Combined sources3
Beta strandi448 – 450Combined sources3
Beta strandi453 – 455Combined sources3
Beta strandi458 – 460Combined sources3
Beta strandi466 – 472Combined sources7
Beta strandi474 – 477Combined sources4
Beta strandi479 – 491Combined sources13
Helixi494 – 496Combined sources3
Helixi500 – 502Combined sources3
Turni515 – 517Combined sources3
Beta strandi518 – 521Combined sources4
Beta strandi524 – 527Combined sources4
Beta strandi530 – 533Combined sources4
Beta strandi538 – 542Combined sources5
Beta strandi545 – 548Combined sources4
Beta strandi553 – 555Combined sources3
Beta strandi559 – 564Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E4UX-ray2.35A/B25-575[»]
2E4VX-ray2.40A/B25-575[»]
2E4WX-ray2.40A/B25-575[»]
2E4XX-ray2.75A/B25-575[»]
2E4YX-ray3.40A/B25-575[»]
ProteinModelPortaliP31422.
SMRiP31422.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31422.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 174Glutamate binding3

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218635.
HOVERGENiHBG107965.
InParanoidiP31422.
KOiK04605.
PhylomeDBiP31422.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001234. GPCR_3_mtglu_rcpt_3.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01053. MTABOTROPC3R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMLTRLQIL MLALFSKGFL LSLGDHNFMR REIKIEGDLV LGGLFPINEK
60 70 80 90 100
GTGTEECGRI NEDRGIQRLE AMLFAIDEIN KDNYLLPGVK LGVHILDTCS
110 120 130 140 150
RDTYALEQSL EFVRASLTKV DEAEYMCPDG SYAIQENIPL LIAGVIGGSY
160 170 180 190 200
SSVSIQVANL LRLFQIPQIS YASTSAKLSD KSRYDYFART VPPDFYQAKA
210 220 230 240 250
MAEILRFFNW TYVSTVASEG DYGETGIEAF EQEARLRNIC IATAEKVGRS
260 270 280 290 300
NIRKSYDSVI RELLQKPNAR VVVLFMRSDD SRELIAAANR VNASFTWVAS
310 320 330 340 350
DGWGAQESIV KGSEHVAYGA ITLELASHPV RQFDRYFQSL NPYNNHRNPW
360 370 380 390 400
FRDFWEQKFQ CSLQNKRNHR QVCDKHLAID SSNYEQESKI MFVVNAVYAM
410 420 430 440 450
AHALHKMQRT LCPNTTKLCD AMKILDGKKL YKEYLLKINF TAPFNPNKGA
460 470 480 490 500
DSIVKFDTFG DGMGRYNVFN LQQTGGKYSY LKVGHWAETL SLDVDSIHWS
510 520 530 540 550
RNSVPTSQCS DPCAPNEMKN MQPGDVCCWI CIPCEPYEYL VDEFTCMDCG
560 570 580 590 600
PGQWPTADLS GCYNLPEDYI KWEDAWAIGP VTIACLGFLC TCIVITVFIK
610 620 630 640 650
HNNTPLVKAS GRELCYILLF GVSLSYCMTF FFIAKPSPVI CALRRLGLGT
660 670 680 690 700
SFAICYSALL TKTNCIARIF DGVKNGAQRP KFISPSSQVF ICLGLILVQI
710 720 730 740 750
VMVSVWLILE TPGTRRYTLP EKRETVILKC NVKDSSMLIS LTYDVVLVIL
760 770 780 790 800
CTVYAFKTRK CPENFNEAKF IGFTMYTTCI IWLAFLPIFY VTSSDYRVQT
810 820 830 840 850
TTMCISVSLS GFVVLGCLFA PKVHIVLFQP QKNVVTHRLH LNRFSVSGTA
860 870
TTYSQSSAST YVPTVCNGRE VLDSTTSSL
Length:879
Mass (Da):98,960
Last modified:July 1, 1993 - v1
Checksum:i3E5965EDD5E6DEED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92076 mRNA. No translation available.
PIRiJH0562.
RefSeqiNP_001099182.1. NM_001105712.1.
UniGeneiRn.41715.

Genome annotation databases

EnsembliENSRNOT00000007572; ENSRNOP00000007572; ENSRNOG00000005519.
GeneIDi24416.
KEGGirno:24416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92076 mRNA. No translation available.
PIRiJH0562.
RefSeqiNP_001099182.1. NM_001105712.1.
UniGeneiRn.41715.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E4UX-ray2.35A/B25-575[»]
2E4VX-ray2.40A/B25-575[»]
2E4WX-ray2.40A/B25-575[»]
2E4XX-ray2.75A/B25-575[»]
2E4YX-ray3.40A/B25-575[»]
ProteinModelPortaliP31422.
SMRiP31422.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246581. 1 interactor.
DIPiDIP-41143N.
IntActiP31422. 4 interactors.
MINTiMINT-1773162.
STRINGi10116.ENSRNOP00000007572.

Chemistry databases

BindingDBiP31422.
ChEMBLiCHEMBL3067.
GuidetoPHARMACOLOGYi291.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP31422.
PhosphoSitePlusiP31422.
UniCarbKBiP31422.

Proteomic databases

PaxDbiP31422.
PRIDEiP31422.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007572; ENSRNOP00000007572; ENSRNOG00000005519.
GeneIDi24416.
KEGGirno:24416.

Organism-specific databases

CTDi2913.
RGDi2744. Grm3.

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218635.
HOVERGENiHBG107965.
InParanoidiP31422.
KOiK04605.
PhylomeDBiP31422.

Enzyme and pathway databases

ReactomeiR-RNO-418594. G alpha (i) signalling events.
R-RNO-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).

Miscellaneous databases

EvolutionaryTraceiP31422.
PROiP31422.

Gene expression databases

BgeeiENSRNOG00000005519.
ExpressionAtlasiP31422. baseline and differential.
GenevisibleiP31422. RN.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001234. GPCR_3_mtglu_rcpt_3.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01053. MTABOTROPC3R.
PR00593. MTABOTROPICR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRM3_RAT
AccessioniPrimary (citable) accession number: P31422
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.