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P31422

- GRM3_RAT

UniProt

P31422 - GRM3_RAT

Protein

Metabotropic glutamate receptor 3

Gene

Grm3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei68 – 681Glutamate
    Binding sitei151 – 1511Glutamate
    Binding sitei222 – 2221Glutamate
    Binding sitei301 – 3011Glutamate
    Binding sitei389 – 3891Glutamate

    GO - Molecular functioni

    1. calcium channel regulator activity Source: Ensembl
    2. group II metabotropic glutamate receptor activity Source: RGD

    GO - Biological processi

    1. adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: GOC
    2. regulation of synaptic transmission Source: RGD
    3. regulation of synaptic transmission, glutamatergic Source: RefGenome
    4. sensory perception of pain Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metabotropic glutamate receptor 3
    Short name:
    mGluR3
    Gene namesi
    Name:Grm3
    Synonyms:Gprc1c, Mglur3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi2744. Grm3.

    Subcellular locationi

    GO - Cellular componenti

    1. astrocyte projection Source: RGD
    2. axon Source: Ensembl
    3. dendritic spine Source: Ensembl
    4. integral component of plasma membrane Source: RefGenome
    5. postsynaptic density Source: Ensembl
    6. postsynaptic membrane Source: Ensembl
    7. presynaptic membrane Source: RefGenome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 879857Metabotropic glutamate receptor 3PRO_0000012929Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi57 ↔ 991 Publication
    Glycosylationi209 – 2091N-linked (GlcNAc...)1 Publication
    Disulfide bondi240 ↔ 5271 Publication
    Glycosylationi292 – 2921N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi361 ↔ 3731 Publication
    Disulfide bondi412 ↔ 4191 Publication
    Glycosylationi414 – 4141N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi509 ↔ 5281 Publication
    Disulfide bondi513 ↔ 5311 Publication
    Disulfide bondi534 ↔ 5461 Publication
    Disulfide bondi549 ↔ 5621 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP31422.
    PRIDEiP31422.

    PTM databases

    PhosphoSiteiP31422.

    Expressioni

    Tissue specificityi

    Is widely distributed in the CNS. Predominant expression is seen in the neuronal cells of the cerebral cortex, dentate gyrus, and glial cells throughout brain regions.

    Gene expression databases

    ArrayExpressiP31422.
    GenevestigatoriP31422.

    Interactioni

    Subunit structurei

    Interacts with GRASP.1 Publication

    Protein-protein interaction databases

    BioGridi246581. 1 interaction.
    IntActiP31422. 4 interactions.
    MINTiMINT-1773162.
    STRINGi10116.ENSRNOP00000007572.

    Structurei

    Secondary structure

    1
    879
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi33 – 353
    Beta strandi38 – 458
    Beta strandi48 – 503
    Beta strandi56 – 605
    Turni62 – 654
    Helixi66 – 8116
    Beta strandi83 – 864
    Beta strandi91 – 977
    Helixi102 – 11413
    Beta strandi142 – 1476
    Helixi151 – 16111
    Helixi162 – 1643
    Beta strandi168 – 1725
    Helixi176 – 1794
    Turni181 – 1833
    Beta strandi187 – 1915
    Helixi194 – 20714
    Beta strandi212 – 2209
    Helixi223 – 23513
    Turni236 – 2383
    Beta strandi240 – 2478
    Helixi253 – 26412
    Beta strandi271 – 2755
    Helixi278 – 29013
    Beta strandi296 – 2994
    Turni301 – 3055
    Helixi307 – 3093
    Turni310 – 3123
    Helixi314 – 3174
    Beta strandi321 – 3255
    Helixi331 – 3388
    Turni342 – 3443
    Helixi351 – 3588
    Beta strandi363 – 3686
    Turni381 – 3833
    Helixi390 – 41122
    Helixi420 – 4223
    Helixi427 – 4348
    Beta strandi438 – 4403
    Beta strandi443 – 4453
    Beta strandi448 – 4503
    Beta strandi453 – 4553
    Beta strandi458 – 4603
    Beta strandi466 – 4727
    Beta strandi474 – 4774
    Beta strandi479 – 49113
    Helixi494 – 4963
    Helixi500 – 5023
    Turni515 – 5173
    Beta strandi518 – 5214
    Beta strandi524 – 5274
    Beta strandi530 – 5334
    Beta strandi538 – 5425
    Beta strandi545 – 5484
    Beta strandi553 – 5553
    Beta strandi559 – 5646

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2E4UX-ray2.35A/B25-575[»]
    2E4VX-ray2.40A/B25-575[»]
    2E4WX-ray2.40A/B25-575[»]
    2E4XX-ray2.75A/B25-575[»]
    2E4YX-ray3.40A/B25-575[»]
    ProteinModelPortaliP31422.
    SMRiP31422. Positions 28-567.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP31422.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 576554ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini600 – 61314CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini635 – 64511ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini665 – 68824CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini710 – 73425ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini757 – 76913CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini793 – 80210ExtracellularSequence Analysis
    Topological domaini829 – 87951CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei577 – 59923Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei614 – 63421Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei646 – 66419Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei689 – 70921Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei735 – 75622Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei770 – 79223Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei803 – 82826Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni172 – 1743Glutamate binding

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG295200.
    GeneTreeiENSGT00750000117490.
    HOGENOMiHOG000218635.
    HOVERGENiHBG107965.
    InParanoidiP31422.
    KOiK04605.
    OMAiCTCIVVT.
    OrthoDBiEOG7Z0JXG.
    PhylomeDBiP31422.

    Family and domain databases

    InterProiIPR001828. ANF_lig-bd_rcpt.
    IPR000337. GPCR_3.
    IPR011500. GPCR_3_9-Cys_dom.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR000162. GPCR_3_mtglu_rcpt.
    IPR001234. GPCR_3_mtglu_rcpt_3.
    IPR028082. Peripla_BP_I.
    [Graphical view]
    PfamiPF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    PF07562. NCD3G. 1 hit.
    [Graphical view]
    PRINTSiPR00248. GPCRMGR.
    PR01053. MTABOTROPC3R.
    PR00593. MTABOTROPICR.
    SUPFAMiSSF53822. SSF53822. 1 hit.
    PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
    PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
    PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31422-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKMLTRLQIL MLALFSKGFL LSLGDHNFMR REIKIEGDLV LGGLFPINEK    50
    GTGTEECGRI NEDRGIQRLE AMLFAIDEIN KDNYLLPGVK LGVHILDTCS 100
    RDTYALEQSL EFVRASLTKV DEAEYMCPDG SYAIQENIPL LIAGVIGGSY 150
    SSVSIQVANL LRLFQIPQIS YASTSAKLSD KSRYDYFART VPPDFYQAKA 200
    MAEILRFFNW TYVSTVASEG DYGETGIEAF EQEARLRNIC IATAEKVGRS 250
    NIRKSYDSVI RELLQKPNAR VVVLFMRSDD SRELIAAANR VNASFTWVAS 300
    DGWGAQESIV KGSEHVAYGA ITLELASHPV RQFDRYFQSL NPYNNHRNPW 350
    FRDFWEQKFQ CSLQNKRNHR QVCDKHLAID SSNYEQESKI MFVVNAVYAM 400
    AHALHKMQRT LCPNTTKLCD AMKILDGKKL YKEYLLKINF TAPFNPNKGA 450
    DSIVKFDTFG DGMGRYNVFN LQQTGGKYSY LKVGHWAETL SLDVDSIHWS 500
    RNSVPTSQCS DPCAPNEMKN MQPGDVCCWI CIPCEPYEYL VDEFTCMDCG 550
    PGQWPTADLS GCYNLPEDYI KWEDAWAIGP VTIACLGFLC TCIVITVFIK 600
    HNNTPLVKAS GRELCYILLF GVSLSYCMTF FFIAKPSPVI CALRRLGLGT 650
    SFAICYSALL TKTNCIARIF DGVKNGAQRP KFISPSSQVF ICLGLILVQI 700
    VMVSVWLILE TPGTRRYTLP EKRETVILKC NVKDSSMLIS LTYDVVLVIL 750
    CTVYAFKTRK CPENFNEAKF IGFTMYTTCI IWLAFLPIFY VTSSDYRVQT 800
    TTMCISVSLS GFVVLGCLFA PKVHIVLFQP QKNVVTHRLH LNRFSVSGTA 850
    TTYSQSSAST YVPTVCNGRE VLDSTTSSL 879
    Length:879
    Mass (Da):98,960
    Last modified:July 1, 1993 - v1
    Checksum:i3E5965EDD5E6DEED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92076 mRNA. No translation available.
    PIRiJH0562.
    RefSeqiNP_001099182.1. NM_001105712.1.
    UniGeneiRn.41715.

    Genome annotation databases

    EnsembliENSRNOT00000007572; ENSRNOP00000007572; ENSRNOG00000005519.
    GeneIDi24416.
    KEGGirno:24416.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92076 mRNA. No translation available.
    PIRi JH0562.
    RefSeqi NP_001099182.1. NM_001105712.1.
    UniGenei Rn.41715.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2E4U X-ray 2.35 A/B 25-575 [» ]
    2E4V X-ray 2.40 A/B 25-575 [» ]
    2E4W X-ray 2.40 A/B 25-575 [» ]
    2E4X X-ray 2.75 A/B 25-575 [» ]
    2E4Y X-ray 3.40 A/B 25-575 [» ]
    ProteinModelPortali P31422.
    SMRi P31422. Positions 28-567.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246581. 1 interaction.
    IntActi P31422. 4 interactions.
    MINTi MINT-1773162.
    STRINGi 10116.ENSRNOP00000007572.

    Chemistry

    BindingDBi P31422.
    ChEMBLi CHEMBL3067.
    GuidetoPHARMACOLOGYi 291.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P31422.

    Proteomic databases

    PaxDbi P31422.
    PRIDEi P31422.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000007572 ; ENSRNOP00000007572 ; ENSRNOG00000005519 .
    GeneIDi 24416.
    KEGGi rno:24416.

    Organism-specific databases

    CTDi 2913.
    RGDi 2744. Grm3.

    Phylogenomic databases

    eggNOGi NOG295200.
    GeneTreei ENSGT00750000117490.
    HOGENOMi HOG000218635.
    HOVERGENi HBG107965.
    InParanoidi P31422.
    KOi K04605.
    OMAi CTCIVVT.
    OrthoDBi EOG7Z0JXG.
    PhylomeDBi P31422.

    Miscellaneous databases

    EvolutionaryTracei P31422.
    NextBioi 603257.

    Gene expression databases

    ArrayExpressi P31422.
    Genevestigatori P31422.

    Family and domain databases

    InterProi IPR001828. ANF_lig-bd_rcpt.
    IPR000337. GPCR_3.
    IPR011500. GPCR_3_9-Cys_dom.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR000162. GPCR_3_mtglu_rcpt.
    IPR001234. GPCR_3_mtglu_rcpt_3.
    IPR028082. Peripla_BP_I.
    [Graphical view ]
    Pfami PF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    PF07562. NCD3G. 1 hit.
    [Graphical view ]
    PRINTSi PR00248. GPCRMGR.
    PR01053. MTABOTROPC3R.
    PR00593. MTABOTROPICR.
    SUPFAMi SSF53822. SSF53822. 1 hit.
    PROSITEi PS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
    PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
    PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A family of metabotropic glutamate receptors."
      Tanabe Y., Masu M., Ishii T., Shigemoto R., Nakanishi S.
      Neuron 8:169-179(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins."
      Kitano J., Kimura K., Yamazaki Y., Soda T., Shigemoto R., Nakajima Y., Nakanishi S.
      J. Neurosci. 22:1280-1289(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GRASP.
    3. "Structures of the extracellular regions of the group II/III metabotropic glutamate receptors."
      Muto T., Tsuchiya D., Morikawa K., Jingami H.
      Proc. Natl. Acad. Sci. U.S.A. 104:3759-3764(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) OF 25-575 IN COMPLEXES WITH GLUTAMATE, GLYCOSYLATION AT ASN-209, DISULFIDE BONDS.

    Entry informationi

    Entry nameiGRM3_RAT
    AccessioniPrimary (citable) accession number: P31422
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3