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P31421 (GRM2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metabotropic glutamate receptor 2

Short name=mGluR2
Gene names
Name:Grm2
Synonyms:Gprc1b, Mglur2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length872 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.

Subunit structure

Interacts with HTR2A By similarity. Interacts with GRASP. Ref.2

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctionsynapse By similarity. Cell projectiondendrite By similarity.

Tissue specificity

Is widely distributed in the CNS and prominent expression is seen in Golgi cells of the cerebellum and some particular neuronal cells in other brain regions.

Sequence similarities

Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
   Cellular componentCell junction
Cell membrane
Cell projection
Membrane
Synapse
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway

Inferred from mutant phenotype PubMed 12837619. Source: GOC

glutamate secretion

Inferred from direct assay PubMed 15993439. Source: UniProtKB

regulation of neurotransmitter secretion

Non-traceable author statement PubMed 15993439. Source: UniProtKB

regulation of synaptic transmission, glutamatergic

Non-traceable author statement PubMed 15993439. Source: UniProtKB

   Cellular_componentaxon

Inferred from direct assay PubMed 15993439. Source: UniProtKB

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

dendrite

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of plasma membrane

Traceable author statement PubMed 15993439. Source: UniProtKB

intracellular

Inferred from direct assay PubMed 15993439. Source: UniProtKB

presynaptic membrane

Inferred from direct assay PubMed 15993439. Source: UniProtKB

   Molecular_functioncalcium channel regulator activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

group II metabotropic glutamate receptor activity

Inferred from mutant phenotype PubMed 12837619. Source: RGD

protein binding

Inferred from physical interaction PubMed 18555800PubMed 19590495. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Htr2aP148423EBI-7090147,EBI-7090176
RANBP9Q96S594EBI-7090147,EBI-636085From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 872854Metabotropic glutamate receptor 2
PRO_0000012926

Regions

Topological domain19 – 567549Extracellular Potential
Transmembrane568 – 59023Helical; Name=1; Potential
Topological domain591 – 60414Cytoplasmic Potential
Transmembrane605 – 62521Helical; Name=2; Potential
Topological domain626 – 63611Extracellular Potential
Transmembrane637 – 65519Helical; Name=3; Potential
Topological domain656 – 67924Cytoplasmic Potential
Transmembrane680 – 70021Helical; Name=4; Potential
Topological domain701 – 72525Extracellular Potential
Transmembrane726 – 74722Helical; Name=5; Potential
Topological domain748 – 76013Cytoplasmic Potential
Transmembrane761 – 78323Helical; Name=6; Potential
Topological domain784 – 79310Extracellular Potential
Transmembrane794 – 81926Helical; Name=7; Potential
Topological domain820 – 87253Cytoplasmic Potential
Region166 – 1683Glutamate binding By similarity
Region677 – 6859Important for interaction with HTR2A By similarity

Sites

Binding site1451Glutamate By similarity
Binding site2161Glutamate By similarity
Binding site2951Glutamate By similarity
Binding site3771Glutamate By similarity

Amino acid modifications

Glycosylation2031N-linked (GlcNAc...) Potential
Glycosylation2861N-linked (GlcNAc...) Potential
Glycosylation3381N-linked (GlcNAc...) Potential
Glycosylation4021N-linked (GlcNAc...) Potential
Glycosylation5471N-linked (GlcNAc...) Potential
Disulfide bond50 ↔ 92 By similarity
Disulfide bond234 ↔ 518 By similarity
Disulfide bond355 ↔ 362 By similarity
Disulfide bond400 ↔ 407 By similarity
Disulfide bond500 ↔ 519 By similarity
Disulfide bond504 ↔ 522 By similarity
Disulfide bond525 ↔ 537 By similarity
Disulfide bond540 ↔ 553 By similarity

Sequences

Sequence LengthMass (Da)Tools
P31421 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 1E74CABD6AD4BED9

FASTA87295,774
        10         20         30         40         50         60 
MESLLGFLAL LLLWGAVAEG PAKKVLTLEG DLVLGGLFPV HQKGGPAEEC GPVNEHRGIQ 

        70         80         90        100        110        120 
RLEAMLFALD RINRDPHLLP GVRLGAHILD SCSKDTHALE QALDFVRASL SRGADGSRHI 

       130        140        150        160        170        180 
CPDGSYATHS DAPTAVTGVI GGSYSDVSIQ VANLLRLFQI PQISYASTSA KLSDKSRYDY 

       190        200        210        220        230        240 
FARTVPPDFF QAKAMAEILR FFNWTYVSTV ASEGDYGETG IEAFELEARA RNICVATSEK 

       250        260        270        280        290        300 
VGRAMSRAAF EGVVRALLQK PSARVAVLFT RSEDARELLA ATQRLNASFT WVASDGWGAL 

       310        320        330        340        350        360 
ESVVAGSERA AEGAITIELA SYPISDFASY FQSLDPWNNS RNPWFREFWE ERFHCSFRQR 

       370        380        390        400        410        420 
DCAAHSLRAV PFEQESKIMF VVNAVYAMAH ALHNMHRALC PNTTHLCDAM RPVNGRRLYK 

       430        440        450        460        470        480 
DFVLNVKFDA PFRPADTDDE VRFDRFGDGI GRYNIFTYLR AGSGRYRYQK VGYWAEGLTL 

       490        500        510        520        530        540 
DTSFIPWASP SAGPLPASRC SEPCLQNEVK SVQPGEVCCW LCIPCQPYEY RLDEFTCADC 

       550        560        570        580        590        600 
GLGYWPNASL TGCFELPQEY IRWGDAWAVG PVTIACLGAL ATLFVLGVFV RHNATPVVKA 

       610        620        630        640        650        660 
SGRELCYILL GGVFLCYCMT FVFIAKPSTA VCTLRRLGLG TAFSVCYSAL LTKTNRIARI 

       670        680        690        700        710        720 
FGGAREGAQR PRFISPASQV AICLALISGQ LLIVAAWLVV EAPGTGKETA PERREVVTLR 

       730        740        750        760        770        780 
CNHRDASMLG SLAYNVLLIA LCTLYAFKTR KCPENFNEAK FIGFTMYTTC IIWLAFLPIF 

       790        800        810        820        830        840 
YVTSSDYRVQ TTTMCVSVSL SGSVVLGCLF APKLHIILFQ PQKNVVSHRA PTSRFGSAAP 

       850        860        870 
RASANLGQGS GSQFVPTVCN GREVVDSTTS SL 

« Hide

References

[1]"A family of metabotropic glutamate receptors."
Tanabe Y., Masu M., Ishii T., Shigemoto R., Nakanishi S.
Neuron 8:169-179(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins."
Kitano J., Kimura K., Yamazaki Y., Soda T., Shigemoto R., Nakajima Y., Nakanishi S.
J. Neurosci. 22:1280-1289(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GRASP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M92075 mRNA. No translation available.
PIRJH0561.
RefSeqNP_001099181.1. NM_001105711.1.
XP_006243766.1. XM_006243704.1.
UniGeneRn.9681.

3D structure databases

ProteinModelPortalP31421.
SMRP31421. Positions 23-558.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246580. 2 interactions.
IntActP31421. 2 interactions.
MINTMINT-146442.
STRING10116.ENSRNOP00000017607.

Chemistry

BindingDBP31421.
ChEMBLCHEMBL2111335.
GuidetoPHARMACOLOGY290.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP31421.

Proteomic databases

PRIDEP31421.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000017607; ENSRNOP00000017607; ENSRNOG00000013171.
GeneID24415.
KEGGrno:24415.
UCSCRGD:2743. rat.

Organism-specific databases

CTD2912.
RGD2743. Grm2.

Phylogenomic databases

eggNOGNOG295200.
GeneTreeENSGT00750000117490.
HOGENOMHOG000218635.
HOVERGENHBG107965.
InParanoidP31421.
KOK04605.
OMAVTLKCNN.
OrthoDBEOG7Z0JXG.
PhylomeDBP31421.
TreeFamTF313240.

Gene expression databases

GenevestigatorP31421.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001458. GPCR_3_mtglu_rcpt_2.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSPR00248. GPCRMGR.
PR01052. MTABOTROPC2R.
PR00593. MTABOTROPICR.
SUPFAMSSF53822. SSF53822. 1 hit.
PROSITEPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio603251.
PROP31421.

Entry information

Entry nameGRM2_RAT
AccessionPrimary (citable) accession number: P31421
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 11, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries