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P31421

- GRM2_RAT

UniProt

P31421 - GRM2_RAT

Protein

Metabotropic glutamate receptor 2

Gene

Grm2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei145 – 1451GlutamateBy similarity
    Binding sitei216 – 2161GlutamateBy similarity
    Binding sitei295 – 2951GlutamateBy similarity
    Binding sitei377 – 3771GlutamateBy similarity

    GO - Molecular functioni

    1. calcium channel regulator activity Source: RefGenome
    2. group II metabotropic glutamate receptor activity Source: RGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway Source: GOC
    2. glutamate secretion Source: UniProtKB
    3. regulation of neurotransmitter secretion Source: UniProtKB
    4. regulation of synaptic transmission, glutamatergic Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metabotropic glutamate receptor 2
    Short name:
    mGluR2
    Gene namesi
    Name:Grm2
    Synonyms:Gprc1b, Mglur2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi2743. Grm2.

    Subcellular locationi

    GO - Cellular componenti

    1. axon Source: UniProtKB
    2. cell junction Source: UniProtKB-KW
    3. dendrite Source: UniProtKB-SubCell
    4. integral component of plasma membrane Source: UniProtKB
    5. intracellular Source: UniProtKB
    6. presynaptic membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 872854Metabotropic glutamate receptor 2PRO_0000012926Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi50 ↔ 92By similarity
    Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi234 ↔ 518By similarity
    Glycosylationi286 – 2861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi338 – 3381N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi355 ↔ 362By similarity
    Disulfide bondi400 ↔ 407By similarity
    Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi500 ↔ 519By similarity
    Disulfide bondi504 ↔ 522By similarity
    Disulfide bondi525 ↔ 537By similarity
    Disulfide bondi540 ↔ 553By similarity
    Glycosylationi547 – 5471N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP31421.

    PTM databases

    PhosphoSiteiP31421.

    Expressioni

    Tissue specificityi

    Is widely distributed in the CNS and prominent expression is seen in Golgi cells of the cerebellum and some particular neuronal cells in other brain regions.

    Gene expression databases

    GenevestigatoriP31421.

    Interactioni

    Subunit structurei

    Interacts with HTR2A By similarity. Interacts with GRASP.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Htr2aP148423EBI-7090147,EBI-7090176
    RANBP9Q96S594EBI-7090147,EBI-636085From a different organism.

    Protein-protein interaction databases

    BioGridi246580. 2 interactions.
    IntActiP31421. 2 interactions.
    MINTiMINT-146442.
    STRINGi10116.ENSRNOP00000017607.

    Structurei

    3D structure databases

    ProteinModelPortaliP31421.
    SMRiP31421. Positions 23-558.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini19 – 567549ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini591 – 60414CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini626 – 63611ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini656 – 67924CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini701 – 72525ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini748 – 76013CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini784 – 79310ExtracellularSequence Analysis
    Topological domaini820 – 87253CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei568 – 59023Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei605 – 62521Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei637 – 65519Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei680 – 70021Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei726 – 74722Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei761 – 78323Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei794 – 81926Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 1683Glutamate bindingBy similarity
    Regioni677 – 6859Important for interaction with HTR2ABy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG295200.
    GeneTreeiENSGT00750000117490.
    HOGENOMiHOG000218635.
    HOVERGENiHBG107965.
    InParanoidiP31421.
    KOiK04605.
    OMAiVTLKCNN.
    OrthoDBiEOG7Z0JXG.
    PhylomeDBiP31421.
    TreeFamiTF313240.

    Family and domain databases

    InterProiIPR001828. ANF_lig-bd_rcpt.
    IPR000337. GPCR_3.
    IPR011500. GPCR_3_9-Cys_dom.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR000162. GPCR_3_mtglu_rcpt.
    IPR001458. GPCR_3_mtglu_rcpt_2.
    IPR028082. Peripla_BP_I.
    [Graphical view]
    PfamiPF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    PF07562. NCD3G. 1 hit.
    [Graphical view]
    PRINTSiPR00248. GPCRMGR.
    PR01052. MTABOTROPC2R.
    PR00593. MTABOTROPICR.
    SUPFAMiSSF53822. SSF53822. 1 hit.
    PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
    PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
    PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31421-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESLLGFLAL LLLWGAVAEG PAKKVLTLEG DLVLGGLFPV HQKGGPAEEC    50
    GPVNEHRGIQ RLEAMLFALD RINRDPHLLP GVRLGAHILD SCSKDTHALE 100
    QALDFVRASL SRGADGSRHI CPDGSYATHS DAPTAVTGVI GGSYSDVSIQ 150
    VANLLRLFQI PQISYASTSA KLSDKSRYDY FARTVPPDFF QAKAMAEILR 200
    FFNWTYVSTV ASEGDYGETG IEAFELEARA RNICVATSEK VGRAMSRAAF 250
    EGVVRALLQK PSARVAVLFT RSEDARELLA ATQRLNASFT WVASDGWGAL 300
    ESVVAGSERA AEGAITIELA SYPISDFASY FQSLDPWNNS RNPWFREFWE 350
    ERFHCSFRQR DCAAHSLRAV PFEQESKIMF VVNAVYAMAH ALHNMHRALC 400
    PNTTHLCDAM RPVNGRRLYK DFVLNVKFDA PFRPADTDDE VRFDRFGDGI 450
    GRYNIFTYLR AGSGRYRYQK VGYWAEGLTL DTSFIPWASP SAGPLPASRC 500
    SEPCLQNEVK SVQPGEVCCW LCIPCQPYEY RLDEFTCADC GLGYWPNASL 550
    TGCFELPQEY IRWGDAWAVG PVTIACLGAL ATLFVLGVFV RHNATPVVKA 600
    SGRELCYILL GGVFLCYCMT FVFIAKPSTA VCTLRRLGLG TAFSVCYSAL 650
    LTKTNRIARI FGGAREGAQR PRFISPASQV AICLALISGQ LLIVAAWLVV 700
    EAPGTGKETA PERREVVTLR CNHRDASMLG SLAYNVLLIA LCTLYAFKTR 750
    KCPENFNEAK FIGFTMYTTC IIWLAFLPIF YVTSSDYRVQ TTTMCVSVSL 800
    SGSVVLGCLF APKLHIILFQ PQKNVVSHRA PTSRFGSAAP RASANLGQGS 850
    GSQFVPTVCN GREVVDSTTS SL 872
    Length:872
    Mass (Da):95,774
    Last modified:July 1, 1993 - v1
    Checksum:i1E74CABD6AD4BED9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92075 mRNA. No translation available.
    PIRiJH0561.
    RefSeqiNP_001099181.1. NM_001105711.1.
    XP_006243766.1. XM_006243704.1.
    UniGeneiRn.9681.

    Genome annotation databases

    EnsembliENSRNOT00000017607; ENSRNOP00000017607; ENSRNOG00000013171.
    GeneIDi24415.
    KEGGirno:24415.
    UCSCiRGD:2743. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92075 mRNA. No translation available.
    PIRi JH0561.
    RefSeqi NP_001099181.1. NM_001105711.1.
    XP_006243766.1. XM_006243704.1.
    UniGenei Rn.9681.

    3D structure databases

    ProteinModelPortali P31421.
    SMRi P31421. Positions 23-558.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246580. 2 interactions.
    IntActi P31421. 2 interactions.
    MINTi MINT-146442.
    STRINGi 10116.ENSRNOP00000017607.

    Chemistry

    BindingDBi P31421.
    ChEMBLi CHEMBL2111335.
    GuidetoPHARMACOLOGYi 290.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P31421.

    Proteomic databases

    PRIDEi P31421.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000017607 ; ENSRNOP00000017607 ; ENSRNOG00000013171 .
    GeneIDi 24415.
    KEGGi rno:24415.
    UCSCi RGD:2743. rat.

    Organism-specific databases

    CTDi 2912.
    RGDi 2743. Grm2.

    Phylogenomic databases

    eggNOGi NOG295200.
    GeneTreei ENSGT00750000117490.
    HOGENOMi HOG000218635.
    HOVERGENi HBG107965.
    InParanoidi P31421.
    KOi K04605.
    OMAi VTLKCNN.
    OrthoDBi EOG7Z0JXG.
    PhylomeDBi P31421.
    TreeFami TF313240.

    Miscellaneous databases

    NextBioi 603251.
    PROi P31421.

    Gene expression databases

    Genevestigatori P31421.

    Family and domain databases

    InterProi IPR001828. ANF_lig-bd_rcpt.
    IPR000337. GPCR_3.
    IPR011500. GPCR_3_9-Cys_dom.
    IPR017978. GPCR_3_C.
    IPR017979. GPCR_3_CS.
    IPR000162. GPCR_3_mtglu_rcpt.
    IPR001458. GPCR_3_mtglu_rcpt_2.
    IPR028082. Peripla_BP_I.
    [Graphical view ]
    Pfami PF00003. 7tm_3. 1 hit.
    PF01094. ANF_receptor. 1 hit.
    PF07562. NCD3G. 1 hit.
    [Graphical view ]
    PRINTSi PR00248. GPCRMGR.
    PR01052. MTABOTROPC2R.
    PR00593. MTABOTROPICR.
    SUPFAMi SSF53822. SSF53822. 1 hit.
    PROSITEi PS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
    PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
    PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
    PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A family of metabotropic glutamate receptors."
      Tanabe Y., Masu M., Ishii T., Shigemoto R., Nakanishi S.
      Neuron 8:169-179(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins."
      Kitano J., Kimura K., Yamazaki Y., Soda T., Shigemoto R., Nakajima Y., Nakanishi S.
      J. Neurosci. 22:1280-1289(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GRASP.

    Entry informationi

    Entry nameiGRM2_RAT
    AccessioniPrimary (citable) accession number: P31421
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3