Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyrophosphate-energized vacuolar membrane proton pump 1

Gene

AVP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to the transtonoplast (from cytosol to vacuole lumen) H+-electrochemical potential difference. It establishes a proton gradient of similar and often greater magnitude than the H+-ATPase on the same membrane. In addition, facilitates auxin transport by modulating apoplastic pH and regulates auxin-mediated developmental processes. Confers tolerance to NaCl and to drought by increasing ion retention.3 Publications

Catalytic activityi

Diphosphate + H2O = 2 phosphate.

Enzyme regulationi

Activated by K+ and Mg2+. Inhibited by Ca2+, N,N'-dicyclohexylcarbodiimide (DCCD), N-ethylmaleimide (NEM) and aminomethylenediphosphonate (AMDP), and, to a lower extent, by fluoride (KF).4 Publications

Kineticsi

  1. KM=110 µM for PPi (at pH 8 and 37 degrees Celsius)2 Publications
  1. Vmax=0.5 µmol/min/mg enzyme (at pH 8 and 37 degrees Celsius)2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei246Important for proton transportBy similarity1
Binding sitei254SubstrateBy similarity1
Metal bindingi257Magnesium 1By similarity1
Metal bindingi257Magnesium 2By similarity1
Metal bindingi261Magnesium 1By similarity1
Metal bindingi287Magnesium 3By similarity1
Sitei291Important for proton transportBy similarity1
Sitei298Important for proton transportBy similarity1
Sitei305Important for proton transportBy similarity1
Metal bindingi511Magnesium 3By similarity1
Metal bindingi538Magnesium 4By similarity1
Metal bindingi695Magnesium 4By similarity1
Metal bindingi731Magnesium 2By similarity1
Binding sitei734SubstrateBy similarity1
Sitei735Important for proton transportBy similarity1
Sitei746Important for proton transportBy similarity1

GO - Molecular functioni

GO - Biological processi

  • auxin polar transport Source: TAIR
  • establishment or maintenance of transmembrane electrochemical gradient Source: TAIR
  • leaf development Source: TAIR
  • proton transport Source: UniProtKB-KW
  • response to salt stress Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G15690-MONOMER.
BRENDAi3.6.1.1. 399.

Protein family/group databases

TCDBi3.A.10.1.1. the h(+), na(+)-translocating pyrophosphatase (m(+)-ppase) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate-energized vacuolar membrane proton pump 1 (EC:3.6.1.1)
Alternative name(s):
Pyrophosphate-energized inorganic pyrophosphatase 1
Short name:
H(+)-PPase 1
Vacuolar proton pyrophosphatase 1
Vacuolar proton pyrophosphatase 3
Gene namesi
Name:AVP1
Synonyms:AVP, AVP-3, AVP3
Ordered Locus Names:At1g15690
ORF Names:F7H2.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G15690.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9IntravacuolarBy similarity9
Transmembranei10 – 36HelicalBy similarityAdd BLAST27
Topological domaini37 – 88CytoplasmicBy similarityAdd BLAST52
Transmembranei89 – 118HelicalBy similarityAdd BLAST30
Topological domaini119 – 139IntravacuolarBy similarityAdd BLAST21
Transmembranei140 – 167HelicalBy similarityAdd BLAST28
Topological domaini168 – 190CytoplasmicBy similarityAdd BLAST23
Transmembranei191 – 220HelicalBy similarityAdd BLAST30
Topological domaini221 – 223IntravacuolarBy similarity3
Transmembranei224 – 252HelicalBy similarityAdd BLAST29
Topological domaini253 – 290CytoplasmicBy similarityAdd BLAST38
Transmembranei291 – 316HelicalBy similarityAdd BLAST26
Topological domaini317 – 324IntravacuolarBy similarity8
Transmembranei325 – 350HelicalBy similarityAdd BLAST26
Topological domaini351 – 358CytoplasmicBy similarity8
Transmembranei359 – 386HelicalBy similarityAdd BLAST28
Topological domaini387 – 405IntravacuolarBy similarityAdd BLAST19
Transmembranei406 – 429HelicalBy similarityAdd BLAST24
Topological domaini430 – 451CytoplasmicBy similarityAdd BLAST22
Transmembranei452 – 476HelicalBy similarityAdd BLAST25
Topological domaini477 – 482IntravacuolarBy similarity6
Transmembranei483 – 509HelicalBy similarityAdd BLAST27
Topological domaini510 – 538CytoplasmicBy similarityAdd BLAST29
Transmembranei539 – 567HelicalBy similarityAdd BLAST29
Topological domaini568 – 577IntravacuolarBy similarity10
Transmembranei578 – 606HelicalBy similarityAdd BLAST29
Topological domaini607 – 635CytoplasmicBy similarityAdd BLAST29
Transmembranei636 – 664HelicalBy similarityAdd BLAST29
Topological domaini665IntravacuolarBy similarity1
Transmembranei666 – 693HelicalBy similarityAdd BLAST28
Topological domaini694 – 736CytoplasmicBy similarityAdd BLAST43
Transmembranei737 – 762HelicalBy similarityAdd BLAST26
Topological domaini763 – 770IntravacuolarBy similarity8

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • endosome membrane Source: TAIR
  • Golgi apparatus Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • mitochondrion Source: TAIR
  • plant-type vacuole Source: TAIR
  • plant-type vacuole membrane Source: TAIR
  • plasma membrane Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi119E → Q: Slight reduction of PPi hydrolysis and H(+) translocation. 1 Publication1
Mutagenesisi229E → D: Slight increased PPi hydrolysis and H(+) translocation. 1 Publication1
Mutagenesisi229E → Q: Slight reduction of PPi hydrolysis and H(+) translocation. 1 Publication1
Mutagenesisi305E → D: Abolishes H(+) translocation and strong reduction of PPi hydrolysis. 1 Publication1
Mutagenesisi305E → Q: Abolishes H(+) translocation and strong reduction of PPi hydrolysis, reduced sensitivity to DCCD. 1 Publication1
Mutagenesisi427E → D: Increases H(+) translocation, normal PPi hydrolysis. 1 Publication1
Mutagenesisi427E → Q: Strong reduction of PPi hydrolysis and H(+) translocation. 1 Publication1
Mutagenesisi504D → E: Abolishes H(+) translocation and strong reduction of PPi hydrolysis. 1 Publication1
Mutagenesisi504D → N: Abolishes H(+) translocation and strong reduction of PPi hydrolysis, reduced sensitivity to DCCD. 1 Publication1
Mutagenesisi573D → N: Increases H(+) translocation, normal PPi hydrolysis. 1 Publication1
Mutagenesisi634C → A or S: Reduced sensitivity to NEM. 1 Publication1
Mutagenesisi667E → Q: Slight reduction of PPi hydrolysis and H(+) translocation. 1 Publication1
Mutagenesisi751E → Q: Slight reduction of PPi hydrolysis and H(+) translocation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002170391 – 770Pyrophosphate-energized vacuolar membrane proton pump 1Add BLAST770

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi128 ↔ 136By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP31414.
PRIDEiP31414.

PTM databases

iPTMnetiP31414.
SwissPalmiP31414.

Expressioni

Tissue specificityi

Ubiquitous (at protein level). Mostly expressed in vascular tissues, meristems and root pericycle.2 Publications

Developmental stagei

Increase of expression in pollen during flower development. Expressed in developing leaves, sepals, petals, stamens and carpels.2 Publications

Inductioni

Repressed by light. Induced by CAMTA1 and/or CAMTA5 in pollen.2 Publications

Gene expression databases

ExpressionAtlasiP31414. baseline and differential.
GenevisibleiP31414. AT.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi23378. 13 interactors.
STRINGi3702.AT1G15690.1.

Structurei

3D structure databases

ProteinModelPortaliP31414.
SMRiP31414.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Has 16 transmembrane helices and a cytoplasmic domain that contains the active site.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFIU. Eukaryota.
COG3808. LUCA.
InParanoidiP31414.
KOiK01507.
OMAiNWICAIS.
OrthoDBiEOG093603U6.
PhylomeDBiP31414.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P31414-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVAPALLPEL WTEILVPICA VIGIAFSLFQ WYVVSRVKLT SDLGASSSGG
60 70 80 90 100
ANNGKNGYGD YLIEEEEGVN DQSVVAKCAE IQTAISEGAT SFLFTEYKYV
110 120 130 140 150
GVFMIFFAAV IFVFLGSVEG FSTDNKPCTY DTTRTCKPAL ATAAFSTIAF
160 170 180 190 200
VLGAVTSVLS GFLGMKIATY ANARTTLEAR KGVGKAFIVA FRSGAVMGFL
210 220 230 240 250
LAASGLLVLY ITINVFKIYY GDDWEGLFEA ITGYGLGGSS MALFGRVGGG
260 270 280 290 300
IYTKAADVGA DLVGKIERNI PEDDPRNPAV IADNVGDNVG DIAGMGSDLF
310 320 330 340 350
GSYAEASCAA LVVASISSFG INHDFTAMCY PLLISSMGIL VCLITTLFAT
360 370 380 390 400
DFFEIKLVKE IEPALKNQLI ISTVIMTVGI AIVSWVGLPT SFTIFNFGTQ
410 420 430 440 450
KVVKNWQLFL CVCVGLWAGL IIGFVTEYYT SNAYSPVQDV ADSCRTGAAT
460 470 480 490 500
NVIFGLALGY KSVIIPIFAI AISIFVSFSF AAMYGVAVAA LGMLSTIATG
510 520 530 540 550
LAIDAYGPIS DNAGGIAEMA GMSHRIRERT DALDAAGNTT AAIGKGFAIG
560 570 580 590 600
SAALVSLALF GAFVSRAGIH TVDVLTPKVI IGLLVGAMLP YWFSAMTMKS
610 620 630 640 650
VGSAALKMVE EVRRQFNTIP GLMEGTAKPD YATCVKISTD ASIKEMIPPG
660 670 680 690 700
CLVMLTPLIV GFFFGVETLS GVLAGSLVSG VQIAISASNT GGAWDNAKKY
710 720 730 740 750
IEAGVSEHAK SLGPKGSEPH KAAVIGDTIG DPLKDTSGPS LNILIKLMAV
760 770
ESLVFAPFFA THGGILFKYF
Length:770
Mass (Da):80,820
Last modified:July 1, 1993 - v1
Checksum:iCE7132B42B299860
GO

Sequence cautioni

The sequence BAD94555 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA79039 differs from that shown. Reason: Frameshift at position 718.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8P → L in BAA32210 (PubMed:11442058).Curated1
Sequence conflicti576T → A in CAA79038 (PubMed:8281187).Curated1
Sequence conflicti584L → P in CAA79038 (PubMed:8281187).Curated1
Sequence conflicti624E → K in AAL57660 (PubMed:14593172).Curated1
Sequence conflicti685I → T in AAL84953 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81892 mRNA. Translation: AAA32754.1.
AB015138 Genomic DNA. Translation: BAA32210.1.
AC034256 Genomic DNA. Translation: AAF82139.1.
CP002684 Genomic DNA. Translation: AEE29349.1.
AY065016 mRNA. Translation: AAL57660.1.
AY078953 mRNA. Translation: AAL84953.1.
BT002481 mRNA. Translation: AAO00841.1.
Z17694 mRNA. Translation: CAA79038.1.
Z17695 mRNA. Translation: CAA79039.1. Frameshift.
AK221989 mRNA. Translation: BAD94555.1. Different initiation.
PIRiA38230.
RefSeqiNP_173021.1. NM_101437.5. [P31414-1]
UniGeneiAt.21942.
At.66944.
At.67102.
At.74973.

Genome annotation databases

EnsemblPlantsiAT1G15690.1; AT1G15690.1; AT1G15690. [P31414-1]
GeneIDi838138.
GrameneiAT1G15690.1; AT1G15690.1; AT1G15690.
KEGGiath:AT1G15690.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81892 mRNA. Translation: AAA32754.1.
AB015138 Genomic DNA. Translation: BAA32210.1.
AC034256 Genomic DNA. Translation: AAF82139.1.
CP002684 Genomic DNA. Translation: AEE29349.1.
AY065016 mRNA. Translation: AAL57660.1.
AY078953 mRNA. Translation: AAL84953.1.
BT002481 mRNA. Translation: AAO00841.1.
Z17694 mRNA. Translation: CAA79038.1.
Z17695 mRNA. Translation: CAA79039.1. Frameshift.
AK221989 mRNA. Translation: BAD94555.1. Different initiation.
PIRiA38230.
RefSeqiNP_173021.1. NM_101437.5. [P31414-1]
UniGeneiAt.21942.
At.66944.
At.67102.
At.74973.

3D structure databases

ProteinModelPortaliP31414.
SMRiP31414.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23378. 13 interactors.
STRINGi3702.AT1G15690.1.

Protein family/group databases

TCDBi3.A.10.1.1. the h(+), na(+)-translocating pyrophosphatase (m(+)-ppase) family.

PTM databases

iPTMnetiP31414.
SwissPalmiP31414.

Proteomic databases

PaxDbiP31414.
PRIDEiP31414.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G15690.1; AT1G15690.1; AT1G15690. [P31414-1]
GeneIDi838138.
GrameneiAT1G15690.1; AT1G15690.1; AT1G15690.
KEGGiath:AT1G15690.

Organism-specific databases

TAIRiAT1G15690.

Phylogenomic databases

eggNOGiENOG410IFIU. Eukaryota.
COG3808. LUCA.
InParanoidiP31414.
KOiK01507.
OMAiNWICAIS.
OrthoDBiEOG093603U6.
PhylomeDBiP31414.

Enzyme and pathway databases

BioCyciARA:AT1G15690-MONOMER.
BRENDAi3.6.1.1. 399.

Miscellaneous databases

PROiP31414.

Gene expression databases

ExpressionAtlasiP31414. baseline and differential.
GenevisibleiP31414. AT.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAVP1_ARATH
AccessioniPrimary (citable) accession number: P31414
Secondary accession number(s): O80390
, Q41919, Q41920, Q56WP6, Q8RY20, Q8VZE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Has few direct interactions with pyrophosphate. Interacts with the substrate via divalent metal cations, such as magnesium ions, that are bound to the pyrophosphate (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.