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Protein

Pyrophosphate-energized vacuolar membrane proton pump 1

Gene

AVP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to the transtonoplast (from cytosol to vacuole lumen) H+-electrochemical potential difference. It establishes a proton gradient of similar and often greater magnitude than the H+-ATPase on the same membrane. In addition, facilitates auxin transport by modulating apoplastic pH and regulates auxin-mediated developmental processes. Confers tolerance to NaCl and to drought by increasing ion retention.3 Publications

Catalytic activityi

Diphosphate + H2O = 2 phosphate.

Enzyme regulationi

Activated by K+ and Mg2+. Inhibited by Ca2+, N,N'-dicyclohexylcarbodiimide (DCCD), N-ethylmaleimide (NEM) and aminomethylenediphosphonate (AMDP), and, to a lower extent, by fluoride (KF).4 Publications

Kineticsi

  1. KM=110 µM for PPi (at pH 8 and 37 degrees Celsius)2 Publications
  1. Vmax=0.5 µmol/min/mg enzyme (at pH 8 and 37 degrees Celsius)2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei246 – 2461Important for proton transportBy similarity
Binding sitei254 – 2541SubstrateBy similarity
Metal bindingi257 – 2571Magnesium 1By similarity
Metal bindingi257 – 2571Magnesium 2By similarity
Metal bindingi261 – 2611Magnesium 1By similarity
Metal bindingi287 – 2871Magnesium 3By similarity
Sitei291 – 2911Important for proton transportBy similarity
Sitei298 – 2981Important for proton transportBy similarity
Sitei305 – 3051Important for proton transportBy similarity
Metal bindingi511 – 5111Magnesium 3By similarity
Metal bindingi538 – 5381Magnesium 4By similarity
Metal bindingi695 – 6951Magnesium 4By similarity
Metal bindingi731 – 7311Magnesium 2By similarity
Binding sitei734 – 7341SubstrateBy similarity
Sitei735 – 7351Important for proton transportBy similarity
Sitei746 – 7461Important for proton transportBy similarity

GO - Molecular functioni

GO - Biological processi

  • auxin polar transport Source: TAIR
  • establishment or maintenance of transmembrane electrochemical gradient Source: TAIR
  • leaf development Source: TAIR
  • proton transport Source: UniProtKB-KW
  • response to salt stress Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G15690-MONOMER.
ARA:GQT-1614-MONOMER.
BRENDAi3.6.1.1. 399.

Protein family/group databases

TCDBi3.A.10.1.1. the h(+), na(+)-translocating pyrophosphatase (m(+)-ppase) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate-energized vacuolar membrane proton pump 1 (EC:3.6.1.1)
Alternative name(s):
Pyrophosphate-energized inorganic pyrophosphatase 1
Short name:
H(+)-PPase 1
Vacuolar proton pyrophosphatase 1
Vacuolar proton pyrophosphatase 3
Gene namesi
Name:AVP1
Synonyms:AVP, AVP-3, AVP3
Ordered Locus Names:At1g15690
ORF Names:F7H2.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G15690.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99IntravacuolarBy similarity
Transmembranei10 – 3627HelicalBy similarityAdd
BLAST
Topological domaini37 – 8852CytoplasmicBy similarityAdd
BLAST
Transmembranei89 – 11830HelicalBy similarityAdd
BLAST
Topological domaini119 – 13921IntravacuolarBy similarityAdd
BLAST
Transmembranei140 – 16728HelicalBy similarityAdd
BLAST
Topological domaini168 – 19023CytoplasmicBy similarityAdd
BLAST
Transmembranei191 – 22030HelicalBy similarityAdd
BLAST
Topological domaini221 – 2233IntravacuolarBy similarity
Transmembranei224 – 25229HelicalBy similarityAdd
BLAST
Topological domaini253 – 29038CytoplasmicBy similarityAdd
BLAST
Transmembranei291 – 31626HelicalBy similarityAdd
BLAST
Topological domaini317 – 3248IntravacuolarBy similarity
Transmembranei325 – 35026HelicalBy similarityAdd
BLAST
Topological domaini351 – 3588CytoplasmicBy similarity
Transmembranei359 – 38628HelicalBy similarityAdd
BLAST
Topological domaini387 – 40519IntravacuolarBy similarityAdd
BLAST
Transmembranei406 – 42924HelicalBy similarityAdd
BLAST
Topological domaini430 – 45122CytoplasmicBy similarityAdd
BLAST
Transmembranei452 – 47625HelicalBy similarityAdd
BLAST
Topological domaini477 – 4826IntravacuolarBy similarity
Transmembranei483 – 50927HelicalBy similarityAdd
BLAST
Topological domaini510 – 53829CytoplasmicBy similarityAdd
BLAST
Transmembranei539 – 56729HelicalBy similarityAdd
BLAST
Topological domaini568 – 57710IntravacuolarBy similarity
Transmembranei578 – 60629HelicalBy similarityAdd
BLAST
Topological domaini607 – 63529CytoplasmicBy similarityAdd
BLAST
Transmembranei636 – 66429HelicalBy similarityAdd
BLAST
Topological domaini665 – 6651IntravacuolarBy similarity
Transmembranei666 – 69328HelicalBy similarityAdd
BLAST
Topological domaini694 – 73643CytoplasmicBy similarityAdd
BLAST
Transmembranei737 – 76226HelicalBy similarityAdd
BLAST
Topological domaini763 – 7708IntravacuolarBy similarity

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • endosome membrane Source: TAIR
  • Golgi apparatus Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • mitochondrion Source: TAIR
  • plant-type vacuole Source: TAIR
  • plant-type vacuole membrane Source: TAIR
  • plasma membrane Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi119 – 1191E → Q: Slight reduction of PPi hydrolysis and H(+) translocation. 1 Publication
Mutagenesisi229 – 2291E → D: Slight increased PPi hydrolysis and H(+) translocation. 1 Publication
Mutagenesisi229 – 2291E → Q: Slight reduction of PPi hydrolysis and H(+) translocation. 1 Publication
Mutagenesisi305 – 3051E → D: Abolishes H(+) translocation and strong reduction of PPi hydrolysis. 1 Publication
Mutagenesisi305 – 3051E → Q: Abolishes H(+) translocation and strong reduction of PPi hydrolysis, reduced sensitivity to DCCD. 1 Publication
Mutagenesisi427 – 4271E → D: Increases H(+) translocation, normal PPi hydrolysis. 1 Publication
Mutagenesisi427 – 4271E → Q: Strong reduction of PPi hydrolysis and H(+) translocation. 1 Publication
Mutagenesisi504 – 5041D → E: Abolishes H(+) translocation and strong reduction of PPi hydrolysis. 1 Publication
Mutagenesisi504 – 5041D → N: Abolishes H(+) translocation and strong reduction of PPi hydrolysis, reduced sensitivity to DCCD. 1 Publication
Mutagenesisi573 – 5731D → N: Increases H(+) translocation, normal PPi hydrolysis. 1 Publication
Mutagenesisi634 – 6341C → A or S: Reduced sensitivity to NEM. 1 Publication
Mutagenesisi667 – 6671E → Q: Slight reduction of PPi hydrolysis and H(+) translocation. 1 Publication
Mutagenesisi751 – 7511E → Q: Slight reduction of PPi hydrolysis and H(+) translocation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 770770Pyrophosphate-energized vacuolar membrane proton pump 1PRO_0000217039Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi128 ↔ 136By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP31414.
PRIDEiP31414.

PTM databases

iPTMnetiP31414.
SwissPalmiP31414.

Expressioni

Tissue specificityi

Ubiquitous (at protein level). Mostly expressed in vascular tissues, meristems and root pericycle.2 Publications

Developmental stagei

Increase of expression in pollen during flower development. Expressed in developing leaves, sepals, petals, stamens and carpels.2 Publications

Inductioni

Repressed by light. Induced by CAMTA1 and/or CAMTA5 in pollen.2 Publications

Gene expression databases

ExpressionAtlasiP31414. baseline and differential.
GenevisibleiP31414. AT.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi23378. 13 interactions.
STRINGi3702.AT1G15690.1.

Structurei

3D structure databases

ProteinModelPortaliP31414.
SMRiP31414. Positions 5-770.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Has 16 transmembrane helices and a cytoplasmic domain that contains the active site.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFIU. Eukaryota.
COG3808. LUCA.
InParanoidiP31414.
OMAiFLAHETK.
PhylomeDBiP31414.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P31414-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVAPALLPEL WTEILVPICA VIGIAFSLFQ WYVVSRVKLT SDLGASSSGG
60 70 80 90 100
ANNGKNGYGD YLIEEEEGVN DQSVVAKCAE IQTAISEGAT SFLFTEYKYV
110 120 130 140 150
GVFMIFFAAV IFVFLGSVEG FSTDNKPCTY DTTRTCKPAL ATAAFSTIAF
160 170 180 190 200
VLGAVTSVLS GFLGMKIATY ANARTTLEAR KGVGKAFIVA FRSGAVMGFL
210 220 230 240 250
LAASGLLVLY ITINVFKIYY GDDWEGLFEA ITGYGLGGSS MALFGRVGGG
260 270 280 290 300
IYTKAADVGA DLVGKIERNI PEDDPRNPAV IADNVGDNVG DIAGMGSDLF
310 320 330 340 350
GSYAEASCAA LVVASISSFG INHDFTAMCY PLLISSMGIL VCLITTLFAT
360 370 380 390 400
DFFEIKLVKE IEPALKNQLI ISTVIMTVGI AIVSWVGLPT SFTIFNFGTQ
410 420 430 440 450
KVVKNWQLFL CVCVGLWAGL IIGFVTEYYT SNAYSPVQDV ADSCRTGAAT
460 470 480 490 500
NVIFGLALGY KSVIIPIFAI AISIFVSFSF AAMYGVAVAA LGMLSTIATG
510 520 530 540 550
LAIDAYGPIS DNAGGIAEMA GMSHRIRERT DALDAAGNTT AAIGKGFAIG
560 570 580 590 600
SAALVSLALF GAFVSRAGIH TVDVLTPKVI IGLLVGAMLP YWFSAMTMKS
610 620 630 640 650
VGSAALKMVE EVRRQFNTIP GLMEGTAKPD YATCVKISTD ASIKEMIPPG
660 670 680 690 700
CLVMLTPLIV GFFFGVETLS GVLAGSLVSG VQIAISASNT GGAWDNAKKY
710 720 730 740 750
IEAGVSEHAK SLGPKGSEPH KAAVIGDTIG DPLKDTSGPS LNILIKLMAV
760 770
ESLVFAPFFA THGGILFKYF
Length:770
Mass (Da):80,820
Last modified:July 1, 1993 - v1
Checksum:iCE7132B42B299860
GO

Sequence cautioni

The sequence BAD94555.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA79039.1 differs from that shown. Reason: Frameshift at position 718. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81P → L in BAA32210 (PubMed:11442058).Curated
Sequence conflicti576 – 5761T → A in CAA79038 (PubMed:8281187).Curated
Sequence conflicti584 – 5841L → P in CAA79038 (PubMed:8281187).Curated
Sequence conflicti624 – 6241E → K in AAL57660 (PubMed:14593172).Curated
Sequence conflicti685 – 6851I → T in AAL84953 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81892 mRNA. Translation: AAA32754.1.
AB015138 Genomic DNA. Translation: BAA32210.1.
AC034256 Genomic DNA. Translation: AAF82139.1.
CP002684 Genomic DNA. Translation: AEE29349.1.
AY065016 mRNA. Translation: AAL57660.1.
AY078953 mRNA. Translation: AAL84953.1.
BT002481 mRNA. Translation: AAO00841.1.
Z17694 mRNA. Translation: CAA79038.1.
Z17695 mRNA. Translation: CAA79039.1. Frameshift.
AK221989 mRNA. Translation: BAD94555.1. Different initiation.
PIRiA38230.
RefSeqiNP_173021.1. NM_101437.4. [P31414-1]
UniGeneiAt.21942.
At.66944.
At.67102.
At.74973.

Genome annotation databases

EnsemblPlantsiAT1G15690.1; AT1G15690.1; AT1G15690. [P31414-1]
GeneIDi838138.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81892 mRNA. Translation: AAA32754.1.
AB015138 Genomic DNA. Translation: BAA32210.1.
AC034256 Genomic DNA. Translation: AAF82139.1.
CP002684 Genomic DNA. Translation: AEE29349.1.
AY065016 mRNA. Translation: AAL57660.1.
AY078953 mRNA. Translation: AAL84953.1.
BT002481 mRNA. Translation: AAO00841.1.
Z17694 mRNA. Translation: CAA79038.1.
Z17695 mRNA. Translation: CAA79039.1. Frameshift.
AK221989 mRNA. Translation: BAD94555.1. Different initiation.
PIRiA38230.
RefSeqiNP_173021.1. NM_101437.4. [P31414-1]
UniGeneiAt.21942.
At.66944.
At.67102.
At.74973.

3D structure databases

ProteinModelPortaliP31414.
SMRiP31414. Positions 5-770.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23378. 13 interactions.
STRINGi3702.AT1G15690.1.

Protein family/group databases

TCDBi3.A.10.1.1. the h(+), na(+)-translocating pyrophosphatase (m(+)-ppase) family.

PTM databases

iPTMnetiP31414.
SwissPalmiP31414.

Proteomic databases

PaxDbiP31414.
PRIDEiP31414.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G15690.1; AT1G15690.1; AT1G15690. [P31414-1]
GeneIDi838138.

Organism-specific databases

TAIRiAT1G15690.

Phylogenomic databases

eggNOGiENOG410IFIU. Eukaryota.
COG3808. LUCA.
InParanoidiP31414.
OMAiFLAHETK.
PhylomeDBiP31414.

Enzyme and pathway databases

BioCyciARA:AT1G15690-MONOMER.
ARA:GQT-1614-MONOMER.
BRENDAi3.6.1.1. 399.

Miscellaneous databases

PROiP31414.

Gene expression databases

ExpressionAtlasiP31414. baseline and differential.
GenevisibleiP31414. AT.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and sequence of cDNA encoding the pyrophosphate-energized vacuolar membrane proton pump of Arabidopsis thaliana."
    Sarafian V., Kim Y., Poole R.J., Rea P.A.
    Proc. Natl. Acad. Sci. U.S.A. 89:1775-1779(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Pollen-specific regulation of vacuolar H(+)-PPase expression by multiple cis-acting elements."
    Mitsuda N., Takeyasu K., Sato M.H.
    Plant Mol. Biol. 46:185-192(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION, DEVELOPMENTAL STAGE.
    Strain: cv. Columbia.
    Tissue: Leaf.
  3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 554-671 AND 716-770.
    Strain: cv. Columbia.
    Tissue: Green siliques.
  7. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 595-770.
    Strain: cv. Columbia.
  8. "Localization of cytosolically oriented maleimide-reactive domain of vacuolar H(+)-pyrophosphatase."
    Zhen R.-G., Kim E.J., Rea P.A.
    J. Biol. Chem. 269:23342-23350(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 625-640, ENZYME REGULATION.
  9. "Heterologous expression of plant vacuolar pyrophosphatase in yeast demonstrates sufficiency of the substrate-binding subunit for proton transport."
    Kim E.J., Zhen R.-G., Rea P.A.
    Proc. Natl. Acad. Sci. U.S.A. 91:6128-6132(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION.
  10. "Site-directed mutagenesis of vacuolar H(+)-pyrophosphatase. Necessity of Cys634 for inhibition by maleimides but not catalysis."
    Kim E.J., Zhen R.-G., Rea P.A.
    J. Biol. Chem. 270:2630-2635(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYS-634, BIOPHYSICOCHEMICAL PROPERTIES.
  11. "Acidic residues necessary for pyrophosphate-energized pumping and inhibition of the vacuolar H(+)-pyrophosphatase by N,N'-dicyclohexylcarbodiimide."
    Zhen R.-G., Kim E.J., Rea P.A.
    J. Biol. Chem. 272:22340-22348(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, MUTAGENESIS OF GLU-119; GLU-229; GLU-305; GLU-427; ASP-504; ASP-573; GLU-667 AND GLU-751.
  12. "AVP2, a sequence-divergent, K(+)-insensitive H(+)-translocating inorganic pyrophosphatase from Arabidopsis."
    Drozdowicz Y.M., Kissinger J.C., Rea P.A.
    Plant Physiol. 123:353-362(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Columbia.
  13. "Drought- and salt-tolerant plants result from overexpression of the AVP1 H(+)-pump."
    Gaxiola R.A., Li J., Undurraga S., Dang L.M., Allen G.J., Alper S.L., Fink G.R.
    Proc. Natl. Acad. Sci. U.S.A. 98:11444-11449(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Arabidopsis CAMTA family proteins enhance V-PPase expression in pollen."
    Mitsuda N., Isono T., Sato M.H.
    Plant Cell Physiol. 44:975-981(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  15. "Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana."
    Shimaoka T., Ohnishi M., Sazuka T., Mitsuhashi N., Hara-Nishimura I., Shimazaki K., Maeshima M., Yokota A., Tomizawa K., Mimura T.
    Plant Cell Physiol. 45:672-683(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  16. Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiAVP1_ARATH
AccessioniPrimary (citable) accession number: P31414
Secondary accession number(s): O80390
, Q41919, Q41920, Q56WP6, Q8RY20, Q8VZE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: February 17, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Has few direct interactions with pyrophosphate. Interacts with the substrate via divalent metal cations, such as magnesium ions, that are bound to the pyrophosphate (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.