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P31412 (VATC_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
V-type proton ATPase subunit C

Short name=V-ATPase subunit C
Alternative name(s):
V-ATPase 42 kDa subunit
Vacuolar proton pump subunit C
Gene names
Name:VMA5
Synonyms:VAT3, VATC
Ordered Locus Names:YKL080W
ORF Names:YKL410
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length392 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit C acts as a flexible stator that holds together the catalytic and the membrane sectors of the enzyme. Reversibly leaves the enzyme after glucose depletion, causing the catalytic subcomplex V1 to detach from the V0 section. Binds ATP and is likely to have a specific function in the catalytic activity of the catalytic sector. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Ref.8

Subunit structure

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d and e). Interacts directly with VMA4. Ref.1 Ref.12

Subcellular location

Vacuole membrane; Peripheral membrane protein Ref.10.

Miscellaneous

Present with 21114 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the V-ATPase C subunit family.

Sequence caution

The sequence AAA34440.1 differs from that shown. Reason: Frameshift at position 25.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 392391V-type proton ATPase subunit C
PRO_0000209358

Amino acid modifications

Modified residue21N-acetylalanine Ref.6

Experimental info

Mutagenesis2551F → A: Is rapidly degraded and disrupts stable ATPase assembly. Ref.9

Secondary structure

................................................ 392
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P31412 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 0CD1B814046C377E

FASTA39244,189
        10         20         30         40         50         60 
MATALYTAND FILISLPQNA QPVTAPGSKT DSWFNETLIG GRAFVSDFKI PEFKIGSLDT 

        70         80         90        100        110        120 
LIVESEELSK VDNQIGASIG KIIEILQGLN ETSTNAYRTL PINNMPVPEY LENFQWQTRK 

       130        140        150        160        170        180 
FKLDKSIKDL ITLISNESSQ LDADVRATYA NYNSAKTNLA AAERKKTGDL SVRSLHDIVK 

       190        200        210        220        230        240 
PEDFVLNSEH LTTVLVAVPK SLKSDFEKSY ETLSKNVVPA SASVIAEDAE YVLFNVHLFK 

       250        260        270        280        290        300 
KNVQEFTTAA REKKFIPREF NYSEELIDQL KKEHDSAASL EQSLRVQLVR LAKTAYVDVF 

       310        320        330        340        350        360 
INWFHIKALR VYVESVLRYG LPPHFNIKII AVPPKNLSKC KSELIDAFGF LGGNAFMKDK 

       370        380        390 
KGKINKQDTS LHQYASLVDT EYEPFVMYII NL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase."
Beltran C., Kopecky J., Pan Y.-C.E., Nelson H., Nelson N.
J. Biol. Chem. 267:774-779(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 99-117, SUBUNIT.
[2]"Isolation of vacuolar membrane H(+)-ATPase-deficient yeast mutants; the VMA5 and VMA4 genes are essential for assembly and activity of the vacuolar H(+)-ATPase."
Ho M.N., Hill K.J., Lindorfer M.A., Stevens T.H.
J. Biol. Chem. 268:221-227(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 99-111; 147-156; 242-251 AND 311-318.
[3]"Sequence analysis of a 10 kb fragment of yeast chromosome XI identifies the SMY1 locus and reveals sequences related to a pre-mRNA splicing factor and vacuolar ATPase subunit C plus a number of unidentified open reading frames."
James C.M., Gent M.E., Indge K.J., Oliver S.G.
Yeast 10:247-255(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]Bienvenut W.V., Peters C.
Submitted (JUN-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-70; 82-98; 126-146; 157-164; 209-215; 242-251; 259-285; 319-328 AND 342-358, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"Evidence for major structural changes in subunit C of the vacuolar ATPase due to nucleotide binding."
Armbruester A., Hohn C., Hermesdorf A., Schumacher K., Boersch M., Grueber G.
FEBS Lett. 579:1961-1967(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 106-115 AND 358-365, NUCLEOTIDE-BINDING.
[8]"The H subunit (Vma13p) of the yeast V-ATPase inhibits the ATPase activity of cytosolic V1 complexes."
Parra K.J., Keenan K.L., Kane P.M.
J. Biol. Chem. 275:21761-21767(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Mutational analysis of the subunit C (Vma5p) of the yeast vacuolar H+-ATPase."
Curtis K.K., Francis S.A., Oluwatosin Y., Kane P.M.
J. Biol. Chem. 277:8979-8988(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF PHE-255.
[10]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]"Defined sites of interaction between subunits E (Vma4p), C (Vma5p), and G (Vma10p) within the stator structure of the vacuolar H(+)-ATPase."
Jones R.P.O., Durose L.J., Findlay J.B.C., Harrison M.A.
Biochemistry 44:3933-3941(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH VMA4.
[13]Erratum
Jones R.P.O., Durose L.J., Findlay J.B.C., Harrison M.A.
Biochemistry 44:11924-11924(2005)
[14]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Crystal structure of yeast V-ATPase subunit C reveals its stator function."
Drory O., Frolow F., Nelson N.
EMBO Rep. 5:1148-1152(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M77143 Genomic DNA. Translation: AAA34440.1. Frameshift.
X75560 Genomic DNA. Translation: CAA53237.1.
Z28080 Genomic DNA. Translation: CAA81917.1.
BK006944 Genomic DNA. Translation: DAA09077.1.
PIRS37905.
RefSeqNP_012843.1. NM_001179646.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1U7LX-ray1.75A1-392[»]
4DL0X-ray2.90C/I158-277[»]
4EFAX-ray2.82C158-277[»]
ProteinModelPortalP31412.
SMRP31412. Positions 5-392.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34052. 77 interactions.
DIPDIP-4701N.
IntActP31412. 16 interactions.
MINTMINT-496270.
STRING4932.YKL080W.

Protein family/group databases

TCDB3.A.2.2.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Proteomic databases

MaxQBP31412.
PaxDbP31412.
PeptideAtlasP31412.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL080W; YKL080W; YKL080W.
GeneID853782.
KEGGsce:YKL080W.

Organism-specific databases

SGDS000001563. VMA5.

Phylogenomic databases

eggNOGCOG5127.
GeneTreeENSGT00390000004263.
HOGENOMHOG000207528.
KOK02148.
OMAQRQYAPL.
OrthoDBEOG7RRFJ7.

Enzyme and pathway databases

BioCycYEAST:G3O-31875-MONOMER.

Gene expression databases

GenevestigatorP31412.

Family and domain databases

InterProIPR004907. ATPase_V1-cplx_csu.
[Graphical view]
PANTHERPTHR10137. PTHR10137. 1 hit.
PfamPF03223. V-ATPase_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP31412.
NextBio974900.

Entry information

Entry nameVATC_YEAST
AccessionPrimary (citable) accession number: P31412
Secondary accession number(s): D6VXK7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 129 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references