P31390 (HRH1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histamine H1 receptor
Short name=H1R
Short name=HH1R

Gene names

Name:

Hrh1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	Potential sites of phosphorylation in the third cytoplasmic loop may play an important role in regulating signal transduction through the receptor molecule.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger Traceable author statement PubMed 12419527. Source: RGD elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: InterPro eosinophil chemotaxis Inferred from electronic annotation. Source: InterPro negative regulation of steroid biosynthetic process Inferred from mutant phenotype PubMed 15917347. Source: RGD positive regulation of nitric oxide biosynthetic process Inferred from direct assay PubMed 12419527. Source: RGD positive regulation of vasoconstriction Inferred from mutant phenotype PubMed 17169617. Source: RGD regulation of cAMP metabolic process Inferred from electronic annotation. Source: InterPro regulation of synaptic transmission Inferred from electronic annotation. Source: InterPro regulation of vascular permeability Inferred from electronic annotation. Source: InterPro response to gravity Inferred from expression pattern PubMed 18607976. Source: RGD synaptic transmission Inferred from direct assay PubMed 12419527. Source: RGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	histamine binding Inferred from mutant phenotype Ref.1. Source: RGD histamine receptor activity Traceable author statement PubMed 12419527. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Histamine H1 receptor		PRO_0000069680
Regions
	Topological domain	1 – 29	29	Extracellular Potential
	Transmembrane	30 – 49	20	Helical; Name=1; Potential
	Topological domain	50 – 63	14	Cytoplasmic Potential
	Transmembrane	64 – 83	20	Helical; Name=2; Potential
	Topological domain	84 – 101	18	Extracellular Potential
	Transmembrane	102 – 123	22	Helical; Name=3; Potential
	Topological domain	124 – 145	22	Cytoplasmic Potential
	Transmembrane	146 – 165	20	Helical; Name=4; Potential
	Topological domain	166 – 189	24	Extracellular Potential
	Transmembrane	190 – 210	21	Helical; Name=5; Potential
	Topological domain	211 – 417	207	Cytoplasmic Potential
	Transmembrane	418 – 437	20	Helical; Name=6; Potential
	Topological domain	438 – 449	12	Extracellular Potential
	Transmembrane	450 – 469	20	Helical; Name=7; Potential
	Topological domain	470 – 486	17	Cytoplasmic Potential
Amino acid modifications
	Lipidation	444	1	S-palmitoyl cysteine Potential
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Glycosylation	18	1	N-linked (GlcNAc...) Potential
	Disulfide bond	100 ↔ 180		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31390 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: E44C02A743A95E88	FASTA	486	55,693
	10 20 30 40 50 60 MSFANTSSTF EDKMCEGNRT AMASPQLLPL VVVLSSISLV TVGLNLLVLY AVHSERKLHT 70 80 90 100 110 120 VGNLYIVSLS VADLIVGAVV MPMNILYLIM TKWSLGRPLC LFWLSMDYVA STASIFSVFI 130 140 150 160 170 180 LCIDRYRSVQ QPLRYLRYRT KTRASATILG AWFFSFLWVI PILGWHHFMP PAPELREDKC 190 200 210 220 230 240 ETDFYNVTWF KIMTAIINFY LPTLLMLWFY VKIYKAVRRH CQHRQLTNGS LPSFSELKLR 250 260 270 280 290 300 SDDTKEGAKK PGRESPWGVL KRPSRDPSVG LDQKSTSEDP KMTSPTVFSQ EGERETRPCF 310 320 330 340 350 360 RLDIMQKQSV AEGDVRGSKA NDQALSQPKM DEQSLNTCRR ISETSEDQTL VDQQSFSRTT 370 380 390 400 410 420 DSDTSIEPGP GRVKSRSGSN SGLDYIKITW KRLRSHSRQY VSGLHLNRER KAAKQLGFIM 430 440 450 460 470 480 AAFILCWIPY FIFFMVIAFC KSCCSEPMHM FTIWLGYINS TLNPLIYPLC NENFKKTFKK ILHIRS

References

[1]	"Genomic cloning of the rat histamine H1 receptor." Fujimoto K., Horio Y., Sugama K., Ito S., Liu Y.Q., Fukui H. Biochem. Biophys. Res. Commun. 190:294-301(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12800 Genomic DNA. Translation: BAA02245.1.
IPI	IPI00211814.
PIR	JC1415.
RefSeq	NP_058714.1. NM_017018.1.
UniGene	Rn.177519. Rn.81032.
3D structure databases
ProteinModelPortal	P31390.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P31390.
Proteomic databases
PaxDb	P31390.
PRIDE	P31390.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24448.
KEGG	rno:24448.
UCSC	RGD:2830. rat.
Organism-specific databases
CTD	3269.
RGD	2830. Hrh1.
Phylogenomic databases
eggNOG	NOG318797.
HOVERGEN	HBG101103.
InParanoid	P31390.
KO	K04149.
OrthoDB	EOG4P2Q2Z.
Gene expression databases
Genevestigator	P31390.
GermOnline	ENSRNOG00000007420. Rattus norvegicus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR000921. Histamine_H1_rcpt. [Graphical view]
PANTHER	PTHR24249:SF56. PTHR24249:SF56. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00530. HISTAMINEH1R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P31390.
ChEMBL	CHEMBL4701.
NextBio	603349.

Entry information

Entry name

HRH1_RAT

Accession

Primary (citable) accession number: P31390

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program