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P31388 (5HT6R_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-hydroxytryptamine receptor 6

Short name=5-HT-6
Short name=5-HT6
Alternative name(s):
ST-B17
Serotonin receptor 6
Gene names
Name:Htr6
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length436 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. It has a high affinity for tricyclic psychotropic drugs.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Localized exclusively in the central nervous system, predominantly in the corpus striatum but also in various limbic and cortical regions.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger

Inferred from mutant phenotype Ref.1. Source: RGD

brain development

Inferred from expression pattern PubMed 9606024. Source: RGD

learning

Inferred from direct assay PubMed 17192775. Source: RGD

long-term synaptic potentiation

Inferred from mutant phenotype PubMed 19660530. Source: RGD

negative regulation of acetylcholine secretion, neurotransmission

Inferred from mutant phenotype PubMed 16987217. Source: RGD

negative regulation of gamma-aminobutyric acid secretion

Inferred from mutant phenotype PubMed 16987217. Source: RGD

negative regulation of glutamate secretion

Inferred from mutant phenotype PubMed 16987217. Source: RGD

positive regulation of dopamine secretion

Inferred from mutant phenotype PubMed 16987217. Source: RGD

positive regulation of gamma-aminobutyric acid secretion

Inferred from mutant phenotype PubMed 19660530. Source: RGD

response to drug

Inferred from expression pattern PubMed 10427606. Source: RGD

response to estradiol

Inferred from expression pattern PubMed 17122082. Source: RGD

serotonin receptor signaling pathway

Inferred from direct assay Ref.1. Source: GOC

   Cellular_componentcilium

Inferred from electronic annotation. Source: Ensembl

dendrite

Inferred from direct assay PubMed 10432491. Source: RGD

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from direct assay PubMed 10924708. Source: RGD

   Molecular_functionserotonin receptor activity

Inferred from direct assay Ref.1. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4364365-hydroxytryptamine receptor 6
PRO_0000068977

Regions

Topological domain1 – 3434Extracellular By similarity
Transmembrane35 – 5723Helical; Name=1; By similarity
Topological domain58 – 647Cytoplasmic By similarity
Transmembrane65 – 8521Helical; Name=2; By similarity
Topological domain86 – 10015Extracellular By similarity
Transmembrane101 – 12222Helical; Name=3; By similarity
Topological domain123 – 14422Cytoplasmic By similarity
Transmembrane145 – 16622Helical; Name=4; By similarity
Topological domain167 – 18418Extracellular By similarity
Transmembrane185 – 20824Helical; Name=5; By similarity
Topological domain209 – 26557Cytoplasmic By similarity
Transmembrane266 – 29025Helical; Name=6; By similarity
Topological domain291 – 2955Extracellular By similarity
Transmembrane296 – 32025Helical; Name=7; By similarity
Topological domain321 – 436116Cytoplasmic By similarity

Amino acid modifications

Glycosylation91N-linked (GlcNAc...) Potential
Disulfide bond99 ↔ 180 By similarity

Experimental info

Sequence conflict571L → V in AAA40618. Ref.1
Sequence conflict336 – 436101PCVHC…HSVPP → HASTVPRSTGQPCLPLHVDL SQRCQTRPQLQQVLALPLPP NSDSDSASGGTSGLQLTAQL LLPGEATRDPPPPTRATTVV NFFVTDSVEPEIRPHPLSSP VN in AAA40618. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P31388 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 6BC00F6A3CBA5FB4

FASTA43646,923
        10         20         30         40         50         60 
MVPEPGPVNS STPAWGPGPP PAPGGSGWVA AALCVVIVLT AAANSLLIVL ICTQPALRNT 

        70         80         90        100        110        120 
SNFFLVSLFT SDLMVGLVVM PPAMLNALYG RWVLARGLCL LWTAFDVMCC SASILNLCLI 

       130        140        150        160        170        180 
SLDRYLLILS PLRYKLRMTA PRALALILGA WSLAALASFL PLLLGWHELG KARTPAPGQC 

       190        200        210        220        230        240 
RLLASLPFVL VASGVTFFLP SGAICFTYCR ILLAARKQAV QVASLTTGTA GQALETLQVP 

       250        260        270        280        290        300 
RTPRPGMESA DSRRLATKHS RKALKASLTL GILLGMFFVT WLPFFVANIA QAVCDCISPG 

       310        320        330        340        350        360 
LFDVLTWLGY CNSTMNPIIY PLFMRDFKRA LGRFLPCVHC PPEHRPALPP PPCGPLTAVP 

       370        380        390        400        410        420 
DQASACSRCC LCLCRQTQIQ TPLQGAPRAC SSQPSFCCLE RPPGTPRHPP GPPLWSTSLS 

       430 
QTLWSLRYGR IHSVPP 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of a novel serotonin receptor with high affinity for tricyclic psychotropic drugs."
Monsma F.J. Jr., Shen Y., Ward R.P., Hamblin M.W., Sibley D.R.
Mol. Pharmacol. 43:320-327(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Corpus striatum.
[2]"A novel rat serotonin (5-HT6) receptor: molecular cloning, localization and stimulation of cAMP accumulation."
Ruat M., Traiffort E., Arrang J.-M., Tardivel-Lacombe J., Diaz J., Leurs R., Schwartz J.-C.
Biochem. Biophys. Res. Commun. 193:268-276(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"A novel rat serotonin (5HT6) receptor."
Martial R.
Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
[4]"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L03202 mRNA. Translation: AAA40618.1.
S62043 mRNA. Translation: AAB26908.1.
L19656 mRNA. Translation: AAA40611.1.
PIRI57942.
JN0591.
RefSeqNP_077341.1. NM_024365.1.
UniGeneRn.10552.

3D structure databases

ProteinModelPortalP31388.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid249039. 1 interaction.
MINTMINT-7214733.

Chemistry

BindingDBP31388.
ChEMBLCHEMBL3372.
GuidetoPHARMACOLOGY11.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP31388.

Proteomic databases

PRIDEP31388.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID64354.
KEGGrno:64354.

Organism-specific databases

CTD3362.
RGD62044. Htr6.

Phylogenomic databases

HOVERGENHBG106962.
KOK04162.

Gene expression databases

GenevestigatorP31388.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613040.
PROP31388.

Entry information

Entry name5HT6R_RAT
AccessionPrimary (citable) accession number: P31388
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries