P31388 (5HT6R_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxytryptamine receptor 6
Short name=5-HT-6
Short name=5-HT6

Alternative name(s):
ST-B17
Serotonin receptor 6

Gene names

Name:

Htr6

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. It has a high affinity for tricyclic psychotropic drugs.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Localized exclusively in the central nervous system, predominantly in the corpus striatum but also in various limbic and cortical regions.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Inferred from mutant phenotype Ref.1. Source: RGD brain development Inferred from expression pattern PubMed 9606024. Source: RGD learning Inferred from direct assay PubMed 17192775. Source: RGD long-term synaptic potentiation Inferred from mutant phenotype PubMed 19660530. Source: RGD negative regulation of acetylcholine secretion Inferred from mutant phenotype PubMed 16987217. Source: RGD negative regulation of gamma-aminobutyric acid secretion Inferred from mutant phenotype PubMed 16987217. Source: RGD negative regulation of glutamate secretion Inferred from mutant phenotype PubMed 16987217. Source: RGD positive regulation of dopamine secretion Inferred from mutant phenotype PubMed 16987217. Source: RGD positive regulation of gamma-aminobutyric acid secretion Inferred from mutant phenotype PubMed 19660530. Source: RGD response to drug Inferred from expression pattern PubMed 10427606. Source: RGD response to estradiol stimulus Inferred from expression pattern PubMed 17122082. Source: RGD
Cellular_component	dendrite Inferred from direct assay PubMed 10432491. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 10924708. Source: RGD
Molecular_function	serotonin receptor activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	5-hydroxytryptamine receptor 6		PRO_0000068977
Regions
	Topological domain	1 – 34	34	Extracellular By similarity
	Transmembrane	35 – 57	23	Helical; Name=1; By similarity
	Topological domain	58 – 64	7	Cytoplasmic By similarity
	Transmembrane	65 – 85	21	Helical; Name=2; By similarity
	Topological domain	86 – 100	15	Extracellular By similarity
	Transmembrane	101 – 122	22	Helical; Name=3; By similarity
	Topological domain	123 – 144	22	Cytoplasmic By similarity
	Transmembrane	145 – 166	22	Helical; Name=4; By similarity
	Topological domain	167 – 184	18	Extracellular By similarity
	Transmembrane	185 – 208	24	Helical; Name=5; By similarity
	Topological domain	209 – 265	57	Cytoplasmic By similarity
	Transmembrane	266 – 290	25	Helical; Name=6; By similarity
	Topological domain	291 – 295	5	Extracellular By similarity
	Transmembrane	296 – 320	25	Helical; Name=7; By similarity
	Topological domain	321 – 436	116	Cytoplasmic By similarity
Amino acid modifications
	Glycosylation	9	1	N-linked (GlcNAc...) Potential
	Disulfide bond	99 ↔ 180		By similarity
Experimental info
	Sequence conflict	57	1	L → V in AAA40618. Ref.1
	Sequence conflict	336 – 436	101	PCVHC…HSVPP → HASTVPRSTGQPCLPLHVDL SQRCQTRPQLQQVLALPLPP NSDSDSASGGTSGLQLTAQL LLPGEATRDPPPPTRATTVV NFFVTDSVEPEIRPHPLSSP VN in AAA40618. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P31388 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 6BC00F6A3CBA5FB4	FASTA	436	46,923
	10 20 30 40 50 60 MVPEPGPVNS STPAWGPGPP PAPGGSGWVA AALCVVIVLT AAANSLLIVL ICTQPALRNT 70 80 90 100 110 120 SNFFLVSLFT SDLMVGLVVM PPAMLNALYG RWVLARGLCL LWTAFDVMCC SASILNLCLI 130 140 150 160 170 180 SLDRYLLILS PLRYKLRMTA PRALALILGA WSLAALASFL PLLLGWHELG KARTPAPGQC 190 200 210 220 230 240 RLLASLPFVL VASGVTFFLP SGAICFTYCR ILLAARKQAV QVASLTTGTA GQALETLQVP 250 260 270 280 290 300 RTPRPGMESA DSRRLATKHS RKALKASLTL GILLGMFFVT WLPFFVANIA QAVCDCISPG 310 320 330 340 350 360 LFDVLTWLGY CNSTMNPIIY PLFMRDFKRA LGRFLPCVHC PPEHRPALPP PPCGPLTAVP 370 380 390 400 410 420 DQASACSRCC LCLCRQTQIQ TPLQGAPRAC SSQPSFCCLE RPPGTPRHPP GPPLWSTSLS 430 QTLWSLRYGR IHSVPP

References

[1]	"Cloning and expression of a novel serotonin receptor with high affinity for tricyclic psychotropic drugs." Monsma F.J. Jr., Shen Y., Ward R.P., Hamblin M.W., Sibley D.R. Mol. Pharmacol. 43:320-327(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Corpus striatum.
[2]	"A novel rat serotonin (5-HT6) receptor: molecular cloning, localization and stimulation of cAMP accumulation." Ruat M., Traiffort E., Arrang J.-M., Tardivel-Lacombe J., Diaz J., Leurs R., Schwartz J.-C. Biochem. Biophys. Res. Commun. 193:268-276(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"A novel rat serotonin (5HT6) receptor." Martial R. Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L03202 mRNA. Translation: AAA40618.1. S62043 mRNA. Translation: AAB26908.1. L19656 mRNA. Translation: AAA40611.1.
IPI	IPI00211802.
PIR	I57942. JN0591.
RefSeq	NP_077341.1. NM_024365.1.
UniGene	Rn.10552.
3D structure databases
ProteinModelPortal	P31388.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P31388.
Proteomic databases
PRIDE	P31388.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	64354.
KEGG	rno:64354.
Organism-specific databases
CTD	3362.
RGD	62044. Htr6.
Phylogenomic databases
HOVERGEN	HBG106962.
KO	K04162.
Gene expression databases
Genevestigator	P31388.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P31388.
ChEMBL	CHEMBL3372.
NextBio	613040.

Entry information

Entry name

5HT6R_RAT

Accession

Primary (citable) accession number: P31388

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program