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P31383 (2AAA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase PP2A regulatory subunit A
Alternative name(s):
PR65
Gene names
Name:TPD3
Ordered Locus Names:YAL016W
ORF Names:FUN32
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length635 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphatase 2A affects a variety of biological processes in the cell such as transcription, cell cycle progression and cellular morphogenesis, and provides an initial identification of critical substrates for this phosphatase. The regulatory subunit may direct the catalytic subunit to distinct, albeit overlapping, subsets of substrates.

Subunit structure

PP2A exists in several trimeric forms, all of which consist of a core composed of a catalytic subunit associated with a 65 kDa regulatory subunit (PR65) (subunit A). The core complex associates with a third, variable subunit (subunit B), which confers distinct properties to the holoenzyme.

Domain

Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.

Miscellaneous

Present with 16900 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the phosphatase 2A regulatory subunit A family.

Contains 15 HEAT repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 635635Protein phosphatase PP2A regulatory subunit A
PRO_0000071414

Regions

Repeat34 – 7239HEAT 1
Repeat73 – 11139HEAT 2
Repeat112 – 15039HEAT 3
Repeat151 – 18939HEAT 4
Repeat190 – 22839HEAT 5
Repeat229 – 27345HEAT 6
Repeat274 – 31643HEAT 7
Repeat317 – 35640HEAT 8
Repeat357 – 39539HEAT 9
Repeat396 – 43439HEAT 10
Repeat435 – 47339HEAT 11
Repeat474 – 51239HEAT 12
Repeat513 – 55341HEAT 13
Repeat554 – 59845HEAT 14
Repeat599 – 63234HEAT 15

Experimental info

Sequence conflict3361V → L in AAA35163. Ref.1
Sequence conflict3561I → T in AAA35163. Ref.1
Sequence conflict3661Q → E in AAA35163. Ref.1
Sequence conflict6111C → F in AAA35163. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P31383 [UniParc].

Last modified October 5, 2010. Version 3.
Checksum: 7ED350FB20633DC8

FASTA63570,907
        10         20         30         40         50         60 
MSGARSTTAG AVPSAATTST TSTTSNSKDS DSNESLYPLA LLMDELKHDD IANRVEAMKK 

        70         80         90        100        110        120 
LDTIALALGP ERTRNELIPF LTEVAQDDED EVFAVLAEQL GKFVPYIGGP QYATILLPVL 

       130        140        150        160        170        180 
EILASAEETL VREKAVDSLN NVAQELSQEQ LFSDFVPLIE HLATADWFSS KVSACGLFKS 

       190        200        210        220        230        240 
VIVRIKDDSL RKNILALYLQ LAQDDTPMVK RAVGKNLPIL IDLLTQNLGL STDEDWDYIS 

       250        260        270        280        290        300 
NIFQKIINDN QDSVKFLAVD CLISILKFFN AKGDESHTQD LLNSAVKLIG DEAWRVRYMA 

       310        320        330        340        350        360 
ADRFSDLASQ FSSNQAYIDE LVQPFLNLCE DNEGDVREAV AKQVSGFAKF LNDPSIILNK 

       370        380        390        400        410        420 
ILPAVQNLSM DESETVRSAL ASKITNIVLL LNKDQVINNF LPILLNMLRD EFPDVRLNII 

       430        440        450        460        470        480 
ASLKVVNDVI GIELLSDSLL PAITELAKDV NWRVRMAIIE YIPILAEQLG MQFFDQQLSD 

       490        500        510        520        530        540 
LCLSWLWDTV YSIREAAVNN LKRLTEIFGS DWCRDEIISR LLKFDLQLLE NFVSRFTILS 

       550        560        570        580        590        600 
ALTTLVPVVS LDVVTEQLLP FISHLADDGV PNIRFNVAKS YAVIVKVLIK DEAKYDALIK 

       610        620        630 
NTILPSLQTL CQDEDVDVKY FAKKSLAECQ ELLKN 

« Hide

References

« Hide 'large scale' references
[1]"Inactivation of the protein phosphatase 2A regulatory subunit A results in morphological and transcriptional defects in Saccharomyces cerevisiae."
van Zyl W., Huang W., Sneddon A.A., Stark M., Camier S., Werner M., Marck C., Sentenac A., Broach J.R.
Mol. Cell. Biol. 12:4946-4959(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes."
Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H.
Genome 36:32-42(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the genes in the FUN38-MAK16-SPO7 region."
Barton A.B., Kaback D.B.
J. Bacteriol. 176:1872-1880(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[4]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M98389 Genomic DNA. Translation: AAA35163.1.
L05146 Genomic DNA. Translation: AAC04941.1.
BK006935 Genomic DNA. Translation: DAA06972.1.
PIRS36718.
RefSeqNP_009386.1. NM_001178161.1.

3D structure databases

ProteinModelPortalP31383.
SMRP31383. Positions 35-626.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31750. 58 interactions.
DIPDIP-2010N.
IntActP31383. 43 interactions.
MINTMINT-402163.
STRING4932.YAL016W.

Proteomic databases

MaxQBP31383.
PaxDbP31383.
PeptideAtlasP31383.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAL016W; YAL016W; YAL016W.
GeneID851217.
KEGGsce:YAL016W.

Organism-specific databases

SGDS000000014. TPD3.

Phylogenomic databases

eggNOGNOG247268.
GeneTreeENSGT00730000110944.
HOGENOMHOG000078539.
KOK03456.
OMAYMAADRF.
OrthoDBEOG7GJ6NN.

Enzyme and pathway databases

BioCycYEAST:G3O-28828-MONOMER.

Gene expression databases

GenevestigatorP31383.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR021133. HEAT_type_2.
[Graphical view]
PfamPF02985. HEAT. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS50077. HEAT_REPEAT. 9 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio968106.

Entry information

Entry name2AAA_YEAST
AccessionPrimary (citable) accession number: P31383
Secondary accession number(s): D6VPK2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 5, 2010
Last modified: May 14, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families