Protein phosphatase PP2A regulatory subunit A - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P31383 (2AAA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein phosphatase PP2A regulatory subunit A

Alternative name(s):
PR65

Gene names

Name:	TPD3
Ordered Locus Names:	YAL016W
ORF Names:	FUN32

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	635 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphatase 2A affects a variety of biological processes in the cell such as transcription, cell cycle progression and cellular morphogenesis, and provides an initial identification of critical substrates for this phosphatase. The regulatory subunit may direct the catalytic subunit to distinct, albeit overlapping, subsets of substrates.
Subunit structure	PP2A exists in several trimeric forms, all of which consist of a core composed of a catalytic subunit associated with a 65 kDa regulatory subunit (PR65) (subunit A). The core complex associates with a third, variable subunit (subunit B), which confers distinct properties to the holoenzyme.
Domain	Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
Miscellaneous	Present with 16900 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the phosphatase 2A regulatory subunit A family. Contains 15 HEAT repeats.

Ontologies

Keywords
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament organization Traceable author statement PubMed 9001215. Source: SGD budding cell bud growth Traceable author statement PubMed 9001215. Source: SGD mitotic spindle assembly checkpoint Inferred from mutant phenotype PubMed 9001215. Source: SGD protein dephosphorylation Traceable author statement PubMed 9001215. Source: SGD regulation of translation Inferred from mutant phenotype PubMed 10329624. Source: SGD
Cellular_component	cellular bud neck Inferred from direct assay PubMed 12388751. Source: SGD cellular bud tip Inferred from direct assay PubMed 12388751. Source: SGD cytoplasm Inferred from direct assay PubMed 12388751. Source: SGD mating projection tip Inferred from direct assay PubMed 12388751. Source: SGD nucleus Inferred from direct assay PubMed 12388751. Source: SGD protein phosphatase type 2A complex Inferred from direct assay PubMed 17550305. Source: SGD spindle pole body Inferred from direct assay PubMed 12388751. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 635

635

Protein phosphatase PP2A regulatory subunit A

PRO_0000071414

Regions

Repeat

34 – 72

HEAT 1

Repeat

73 – 111

HEAT 2

Repeat

112 – 150

HEAT 3

Repeat

151 – 189

HEAT 4

Repeat

190 – 228

HEAT 5

Repeat

229 – 273

HEAT 6

Repeat

274 – 316

HEAT 7

Repeat

317 – 356

HEAT 8

Repeat

357 – 395

HEAT 9

Repeat

396 – 434

HEAT 10

Repeat

435 – 473

HEAT 11

Repeat

474 – 512

HEAT 12

Repeat

513 – 553

HEAT 13

Repeat

554 – 598

HEAT 14

Repeat

599 – 632

HEAT 15

Experimental info

Sequence conflict

336

V → L in AAA35163. Ref.1

Sequence conflict

356

I → T in AAA35163. Ref.1

Sequence conflict

366

Q → E in AAA35163. Ref.1

Sequence conflict

611

C → F in AAA35163. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P31383 [UniParc].

Last modified October 5, 2010. Version 3.
Checksum: 7ED350FB20633DC8
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FASTA

635

70,907

        10         20         30         40         50         60 
MSGARSTTAG AVPSAATTST TSTTSNSKDS DSNESLYPLA LLMDELKHDD IANRVEAMKK 

        70         80         90        100        110        120 
LDTIALALGP ERTRNELIPF LTEVAQDDED EVFAVLAEQL GKFVPYIGGP QYATILLPVL 

       130        140        150        160        170        180 
EILASAEETL VREKAVDSLN NVAQELSQEQ LFSDFVPLIE HLATADWFSS KVSACGLFKS 

       190        200        210        220        230        240 
VIVRIKDDSL RKNILALYLQ LAQDDTPMVK RAVGKNLPIL IDLLTQNLGL STDEDWDYIS 

       250        260        270        280        290        300 
NIFQKIINDN QDSVKFLAVD CLISILKFFN AKGDESHTQD LLNSAVKLIG DEAWRVRYMA 

       310        320        330        340        350        360 
ADRFSDLASQ FSSNQAYIDE LVQPFLNLCE DNEGDVREAV AKQVSGFAKF LNDPSIILNK 

       370        380        390        400        410        420 
ILPAVQNLSM DESETVRSAL ASKITNIVLL LNKDQVINNF LPILLNMLRD EFPDVRLNII 

       430        440        450        460        470        480 
ASLKVVNDVI GIELLSDSLL PAITELAKDV NWRVRMAIIE YIPILAEQLG MQFFDQQLSD 

       490        500        510        520        530        540 
LCLSWLWDTV YSIREAAVNN LKRLTEIFGS DWCRDEIISR LLKFDLQLLE NFVSRFTILS 

       550        560        570        580        590        600 
ALTTLVPVVS LDVVTEQLLP FISHLADDGV PNIRFNVAKS YAVIVKVLIK DEAKYDALIK 

       610        620        630 
NTILPSLQTL CQDEDVDVKY FAKKSLAECQ ELLKN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Inactivation of the protein phosphatase 2A regulatory subunit A results in morphological and transcriptional defects in Saccharomyces cerevisiae." van Zyl W., Huang W., Sneddon A.A., Stark M., Camier S., Werner M., Marck C., Sentenac A., Broach J.R. Mol. Cell. Biol. 12:4946-4959(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes." Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H. Genome 36:32-42(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the genes in the FUN38-MAK16-SPO7 region." Barton A.B., Kaback D.B. J. Bacteriol. 176:1872-1880(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M98389 Genomic DNA. Translation: AAA35163.1. L05146 Genomic DNA. Translation: AAC04941.1. BK006935 Genomic DNA. Translation: DAA06972.1.
PIR	S36718.
RefSeq	NP_009386.1. NM_001178161.1.
3D structure databases
ProteinModelPortal	P31383.
SMR	P31383. Positions 35-626.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2010N.
IntAct	P31383. 43 interactions.
MINT	MINT-402163.
STRING	4932.YAL016W.
Proteomic databases
PaxDb	P31383.
PeptideAtlas	P31383.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL016W; YAL016W; YAL016W.
GeneID	851217.
KEGG	sce:YAL016W.
Organism-specific databases
SGD	S000000014. TPD3.
Phylogenomic databases
eggNOG	NOG247268.
GeneTree	ENSGT00670000097819.
HOGENOM	HOG000078539.
KO	K03456.
OMA	VENMIRP.
OrthoDB	EOG454D74.
Gene expression databases
Genevestigator	P31383.
GermOnline	YAL016W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000357. HEAT. IPR021133. HEAT_type_2. [Graphical view]
Pfam	PF02985. HEAT. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50077. HEAT_REPEAT. 9 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	968106.

Entry information

Entry name

2AAA_YEAST

Accession

Primary (citable) accession number: P31383
Secondary accession number(s): D6VPK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	October 5, 2010
Last modified:	May 1, 2013
This is version 115 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents