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Protein

Protein phosphatase PP2A regulatory subunit A

Gene

TPD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatase 2A affects a variety of biological processes in the cell such as transcription, cell cycle progression and cellular morphogenesis, and provides an initial identification of critical substrates for this phosphatase. The regulatory subunit may direct the catalytic subunit to distinct, albeit overlapping, subsets of substrates.

GO - Biological processi

  • mitotic spindle assembly checkpoint Source: SGD
  • protein dephosphorylation Source: GOC
  • regulation of translation Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-28828-MONOMER.
ReactomeiR-SCE-198753. ERK/MAPK targets.
R-SCE-202670. ERKs are inactivated.
R-SCE-389513. CTLA4 inhibitory signaling.
R-SCE-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase PP2A regulatory subunit A
Alternative name(s):
PR65
Gene namesi
Name:TPD3
Ordered Locus Names:YAL016W
ORF Names:FUN32
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL016W.
SGDiS000000014. TPD3.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • cytoplasm Source: SGD
  • mating projection tip Source: SGD
  • nucleus Source: SGD
  • protein phosphatase type 2A complex Source: SGD
  • spindle pole body Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000714141 – 635Protein phosphatase PP2A regulatory subunit AAdd BLAST635

Proteomic databases

MaxQBiP31383.
PRIDEiP31383.

Interactioni

Subunit structurei

PP2A exists in several trimeric forms, all of which consist of a core composed of a catalytic subunit associated with a 65 kDa regulatory subunit (PR65) (subunit A). The core complex associates with a third, variable subunit (subunit B), which confers distinct properties to the holoenzyme.

Protein-protein interaction databases

BioGridi31750. 60 interactors.
DIPiDIP-2010N.
IntActiP31383. 43 interactors.
MINTiMINT-402163.

Structurei

3D structure databases

ProteinModelPortaliP31383.
SMRiP31383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati34 – 72HEAT 1Add BLAST39
Repeati73 – 111HEAT 2Add BLAST39
Repeati112 – 150HEAT 3Add BLAST39
Repeati151 – 189HEAT 4Add BLAST39
Repeati190 – 228HEAT 5Add BLAST39
Repeati229 – 273HEAT 6Add BLAST45
Repeati274 – 316HEAT 7Add BLAST43
Repeati317 – 356HEAT 8Add BLAST40
Repeati357 – 395HEAT 9Add BLAST39
Repeati396 – 434HEAT 10Add BLAST39
Repeati435 – 473HEAT 11Add BLAST39
Repeati474 – 512HEAT 12Add BLAST39
Repeati513 – 553HEAT 13Add BLAST41
Repeati554 – 598HEAT 14Add BLAST45
Repeati599 – 632HEAT 15Add BLAST34

Domaini

Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.

Sequence similaritiesi

Contains 15 HEAT repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00730000110944.
HOGENOMiHOG000078539.
InParanoidiP31383.
KOiK03456.
OMAiMSNILPC.
OrthoDBiEOG092C1A2V.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 9 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGARSTTAG AVPSAATTST TSTTSNSKDS DSNESLYPLA LLMDELKHDD
60 70 80 90 100
IANRVEAMKK LDTIALALGP ERTRNELIPF LTEVAQDDED EVFAVLAEQL
110 120 130 140 150
GKFVPYIGGP QYATILLPVL EILASAEETL VREKAVDSLN NVAQELSQEQ
160 170 180 190 200
LFSDFVPLIE HLATADWFSS KVSACGLFKS VIVRIKDDSL RKNILALYLQ
210 220 230 240 250
LAQDDTPMVK RAVGKNLPIL IDLLTQNLGL STDEDWDYIS NIFQKIINDN
260 270 280 290 300
QDSVKFLAVD CLISILKFFN AKGDESHTQD LLNSAVKLIG DEAWRVRYMA
310 320 330 340 350
ADRFSDLASQ FSSNQAYIDE LVQPFLNLCE DNEGDVREAV AKQVSGFAKF
360 370 380 390 400
LNDPSIILNK ILPAVQNLSM DESETVRSAL ASKITNIVLL LNKDQVINNF
410 420 430 440 450
LPILLNMLRD EFPDVRLNII ASLKVVNDVI GIELLSDSLL PAITELAKDV
460 470 480 490 500
NWRVRMAIIE YIPILAEQLG MQFFDQQLSD LCLSWLWDTV YSIREAAVNN
510 520 530 540 550
LKRLTEIFGS DWCRDEIISR LLKFDLQLLE NFVSRFTILS ALTTLVPVVS
560 570 580 590 600
LDVVTEQLLP FISHLADDGV PNIRFNVAKS YAVIVKVLIK DEAKYDALIK
610 620 630
NTILPSLQTL CQDEDVDVKY FAKKSLAECQ ELLKN
Length:635
Mass (Da):70,907
Last modified:October 5, 2010 - v3
Checksum:i7ED350FB20633DC8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti336V → L in AAA35163 (PubMed:1328868).Curated1
Sequence conflicti356I → T in AAA35163 (PubMed:1328868).Curated1
Sequence conflicti366Q → E in AAA35163 (PubMed:1328868).Curated1
Sequence conflicti611C → F in AAA35163 (PubMed:1328868).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98389 Genomic DNA. Translation: AAA35163.1.
L05146 Genomic DNA. Translation: AAC04941.1.
BK006935 Genomic DNA. Translation: DAA06972.1.
PIRiS36718.
RefSeqiNP_009386.1. NM_001178161.1.

Genome annotation databases

EnsemblFungiiYAL016W; YAL016W; YAL016W.
GeneIDi851217.
KEGGisce:YAL016W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98389 Genomic DNA. Translation: AAA35163.1.
L05146 Genomic DNA. Translation: AAC04941.1.
BK006935 Genomic DNA. Translation: DAA06972.1.
PIRiS36718.
RefSeqiNP_009386.1. NM_001178161.1.

3D structure databases

ProteinModelPortaliP31383.
SMRiP31383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31750. 60 interactors.
DIPiDIP-2010N.
IntActiP31383. 43 interactors.
MINTiMINT-402163.

Proteomic databases

MaxQBiP31383.
PRIDEiP31383.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL016W; YAL016W; YAL016W.
GeneIDi851217.
KEGGisce:YAL016W.

Organism-specific databases

EuPathDBiFungiDB:YAL016W.
SGDiS000000014. TPD3.

Phylogenomic databases

GeneTreeiENSGT00730000110944.
HOGENOMiHOG000078539.
InParanoidiP31383.
KOiK03456.
OMAiMSNILPC.
OrthoDBiEOG092C1A2V.

Enzyme and pathway databases

BioCyciYEAST:G3O-28828-MONOMER.
ReactomeiR-SCE-198753. ERK/MAPK targets.
R-SCE-202670. ERKs are inactivated.
R-SCE-389513. CTLA4 inhibitory signaling.
R-SCE-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-SCE-69273. Cyclin A/B1 associated events during G2/M transition.

Miscellaneous databases

PROiP31383.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021133. HEAT_type_2.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 9 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei2AAA_YEAST
AccessioniPrimary (citable) accession number: P31383
Secondary accession number(s): D6VPK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 5, 2010
Last modified: November 2, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 16900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.