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P31383

- 2AAA_YEAST

UniProt

P31383 - 2AAA_YEAST

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Protein

Protein phosphatase PP2A regulatory subunit A

Gene
TPD3, YAL016W, FUN32
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Phosphatase 2A affects a variety of biological processes in the cell such as transcription, cell cycle progression and cellular morphogenesis, and provides an initial identification of critical substrates for this phosphatase. The regulatory subunit may direct the catalytic subunit to distinct, albeit overlapping, subsets of substrates.

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. mitotic spindle assembly checkpoint Source: SGD
  3. regulation of translation Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-28828-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase PP2A regulatory subunit A
Alternative name(s):
PR65
Gene namesi
Name:TPD3
Ordered Locus Names:YAL016W
ORF Names:FUN32
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

SGDiS000000014. TPD3.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
  2. cellular bud tip Source: SGD
  3. cytoplasm Source: SGD
  4. mating projection tip Source: SGD
  5. nucleus Source: SGD
  6. protein phosphatase type 2A complex Source: SGD
  7. spindle pole body Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 635635Protein phosphatase PP2A regulatory subunit APRO_0000071414Add
BLAST

Proteomic databases

MaxQBiP31383.
PaxDbiP31383.
PeptideAtlasiP31383.

Expressioni

Gene expression databases

GenevestigatoriP31383.

Interactioni

Subunit structurei

PP2A exists in several trimeric forms, all of which consist of a core composed of a catalytic subunit associated with a 65 kDa regulatory subunit (PR65) (subunit A). The core complex associates with a third, variable subunit (subunit B), which confers distinct properties to the holoenzyme.

Protein-protein interaction databases

BioGridi31750. 58 interactions.
DIPiDIP-2010N.
IntActiP31383. 43 interactions.
MINTiMINT-402163.
STRINGi4932.YAL016W.

Structurei

3D structure databases

ProteinModelPortaliP31383.
SMRiP31383. Positions 35-626.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati34 – 7239HEAT 1Add
BLAST
Repeati73 – 11139HEAT 2Add
BLAST
Repeati112 – 15039HEAT 3Add
BLAST
Repeati151 – 18939HEAT 4Add
BLAST
Repeati190 – 22839HEAT 5Add
BLAST
Repeati229 – 27345HEAT 6Add
BLAST
Repeati274 – 31643HEAT 7Add
BLAST
Repeati317 – 35640HEAT 8Add
BLAST
Repeati357 – 39539HEAT 9Add
BLAST
Repeati396 – 43439HEAT 10Add
BLAST
Repeati435 – 47339HEAT 11Add
BLAST
Repeati474 – 51239HEAT 12Add
BLAST
Repeati513 – 55341HEAT 13Add
BLAST
Repeati554 – 59845HEAT 14Add
BLAST
Repeati599 – 63234HEAT 15Add
BLAST

Domaini

Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.

Sequence similaritiesi

Contains 15 HEAT repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG247268.
GeneTreeiENSGT00730000110944.
HOGENOMiHOG000078539.
KOiK03456.
OMAiYMAADRF.
OrthoDBiEOG7GJ6NN.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR021133. HEAT_type_2.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 9 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31383-1 [UniParc]FASTAAdd to Basket

« Hide

MSGARSTTAG AVPSAATTST TSTTSNSKDS DSNESLYPLA LLMDELKHDD    50
IANRVEAMKK LDTIALALGP ERTRNELIPF LTEVAQDDED EVFAVLAEQL 100
GKFVPYIGGP QYATILLPVL EILASAEETL VREKAVDSLN NVAQELSQEQ 150
LFSDFVPLIE HLATADWFSS KVSACGLFKS VIVRIKDDSL RKNILALYLQ 200
LAQDDTPMVK RAVGKNLPIL IDLLTQNLGL STDEDWDYIS NIFQKIINDN 250
QDSVKFLAVD CLISILKFFN AKGDESHTQD LLNSAVKLIG DEAWRVRYMA 300
ADRFSDLASQ FSSNQAYIDE LVQPFLNLCE DNEGDVREAV AKQVSGFAKF 350
LNDPSIILNK ILPAVQNLSM DESETVRSAL ASKITNIVLL LNKDQVINNF 400
LPILLNMLRD EFPDVRLNII ASLKVVNDVI GIELLSDSLL PAITELAKDV 450
NWRVRMAIIE YIPILAEQLG MQFFDQQLSD LCLSWLWDTV YSIREAAVNN 500
LKRLTEIFGS DWCRDEIISR LLKFDLQLLE NFVSRFTILS ALTTLVPVVS 550
LDVVTEQLLP FISHLADDGV PNIRFNVAKS YAVIVKVLIK DEAKYDALIK 600
NTILPSLQTL CQDEDVDVKY FAKKSLAECQ ELLKN 635
Length:635
Mass (Da):70,907
Last modified:October 5, 2010 - v3
Checksum:i7ED350FB20633DC8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti336 – 3361V → L in AAA35163. 1 Publication
Sequence conflicti356 – 3561I → T in AAA35163. 1 Publication
Sequence conflicti366 – 3661Q → E in AAA35163. 1 Publication
Sequence conflicti611 – 6111C → F in AAA35163. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M98389 Genomic DNA. Translation: AAA35163.1.
L05146 Genomic DNA. Translation: AAC04941.1.
BK006935 Genomic DNA. Translation: DAA06972.1.
PIRiS36718.
RefSeqiNP_009386.1. NM_001178161.1.

Genome annotation databases

EnsemblFungiiYAL016W; YAL016W; YAL016W.
GeneIDi851217.
KEGGisce:YAL016W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M98389 Genomic DNA. Translation: AAA35163.1 .
L05146 Genomic DNA. Translation: AAC04941.1 .
BK006935 Genomic DNA. Translation: DAA06972.1 .
PIRi S36718.
RefSeqi NP_009386.1. NM_001178161.1.

3D structure databases

ProteinModelPortali P31383.
SMRi P31383. Positions 35-626.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31750. 58 interactions.
DIPi DIP-2010N.
IntActi P31383. 43 interactions.
MINTi MINT-402163.
STRINGi 4932.YAL016W.

Proteomic databases

MaxQBi P31383.
PaxDbi P31383.
PeptideAtlasi P31383.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL016W ; YAL016W ; YAL016W .
GeneIDi 851217.
KEGGi sce:YAL016W.

Organism-specific databases

SGDi S000000014. TPD3.

Phylogenomic databases

eggNOGi NOG247268.
GeneTreei ENSGT00730000110944.
HOGENOMi HOG000078539.
KOi K03456.
OMAi YMAADRF.
OrthoDBi EOG7GJ6NN.

Enzyme and pathway databases

BioCyci YEAST:G3O-28828-MONOMER.

Miscellaneous databases

NextBioi 968106.

Gene expression databases

Genevestigatori P31383.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR021133. HEAT_type_2.
[Graphical view ]
Pfami PF02985. HEAT. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS50077. HEAT_REPEAT. 9 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Inactivation of the protein phosphatase 2A regulatory subunit A results in morphological and transcriptional defects in Saccharomyces cerevisiae."
    van Zyl W., Huang W., Sneddon A.A., Stark M., Camier S., Werner M., Marck C., Sentenac A., Broach J.R.
    Mol. Cell. Biol. 12:4946-4959(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes."
    Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H.
    Genome 36:32-42(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  3. "Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the genes in the FUN38-MAK16-SPO7 region."
    Barton A.B., Kaback D.B.
    J. Bacteriol. 176:1872-1880(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry namei2AAA_YEAST
AccessioniPrimary (citable) accession number: P31383
Secondary accession number(s): D6VPK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 5, 2010
Last modified: May 14, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 16900 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

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