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P31383

- 2AAA_YEAST

UniProt

P31383 - 2AAA_YEAST

Protein

Protein phosphatase PP2A regulatory subunit A

Gene

TPD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 3 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Phosphatase 2A affects a variety of biological processes in the cell such as transcription, cell cycle progression and cellular morphogenesis, and provides an initial identification of critical substrates for this phosphatase. The regulatory subunit may direct the catalytic subunit to distinct, albeit overlapping, subsets of substrates.

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. mitotic spindle assembly checkpoint Source: SGD
    3. regulation of translation Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28828-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase PP2A regulatory subunit A
    Alternative name(s):
    PR65
    Gene namesi
    Name:TPD3
    Ordered Locus Names:YAL016W
    ORF Names:FUN32
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    SGDiS000000014. TPD3.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cellular bud tip Source: SGD
    3. cytoplasm Source: SGD
    4. mating projection tip Source: SGD
    5. nucleus Source: SGD
    6. protein phosphatase type 2A complex Source: SGD
    7. spindle pole body Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 635635Protein phosphatase PP2A regulatory subunit APRO_0000071414Add
    BLAST

    Proteomic databases

    MaxQBiP31383.
    PaxDbiP31383.
    PeptideAtlasiP31383.

    Expressioni

    Gene expression databases

    GenevestigatoriP31383.

    Interactioni

    Subunit structurei

    PP2A exists in several trimeric forms, all of which consist of a core composed of a catalytic subunit associated with a 65 kDa regulatory subunit (PR65) (subunit A). The core complex associates with a third, variable subunit (subunit B), which confers distinct properties to the holoenzyme.

    Protein-protein interaction databases

    BioGridi31750. 58 interactions.
    DIPiDIP-2010N.
    IntActiP31383. 43 interactions.
    MINTiMINT-402163.
    STRINGi4932.YAL016W.

    Structurei

    3D structure databases

    ProteinModelPortaliP31383.
    SMRiP31383. Positions 35-626.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati34 – 7239HEAT 1Add
    BLAST
    Repeati73 – 11139HEAT 2Add
    BLAST
    Repeati112 – 15039HEAT 3Add
    BLAST
    Repeati151 – 18939HEAT 4Add
    BLAST
    Repeati190 – 22839HEAT 5Add
    BLAST
    Repeati229 – 27345HEAT 6Add
    BLAST
    Repeati274 – 31643HEAT 7Add
    BLAST
    Repeati317 – 35640HEAT 8Add
    BLAST
    Repeati357 – 39539HEAT 9Add
    BLAST
    Repeati396 – 43439HEAT 10Add
    BLAST
    Repeati435 – 47339HEAT 11Add
    BLAST
    Repeati474 – 51239HEAT 12Add
    BLAST
    Repeati513 – 55341HEAT 13Add
    BLAST
    Repeati554 – 59845HEAT 14Add
    BLAST
    Repeati599 – 63234HEAT 15Add
    BLAST

    Domaini

    Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.

    Sequence similaritiesi

    Contains 15 HEAT repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG247268.
    GeneTreeiENSGT00730000110944.
    HOGENOMiHOG000078539.
    KOiK03456.
    OMAiYMAADRF.
    OrthoDBiEOG7GJ6NN.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000357. HEAT.
    IPR021133. HEAT_type_2.
    [Graphical view]
    PfamiPF02985. HEAT. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS50077. HEAT_REPEAT. 9 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31383-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGARSTTAG AVPSAATTST TSTTSNSKDS DSNESLYPLA LLMDELKHDD    50
    IANRVEAMKK LDTIALALGP ERTRNELIPF LTEVAQDDED EVFAVLAEQL 100
    GKFVPYIGGP QYATILLPVL EILASAEETL VREKAVDSLN NVAQELSQEQ 150
    LFSDFVPLIE HLATADWFSS KVSACGLFKS VIVRIKDDSL RKNILALYLQ 200
    LAQDDTPMVK RAVGKNLPIL IDLLTQNLGL STDEDWDYIS NIFQKIINDN 250
    QDSVKFLAVD CLISILKFFN AKGDESHTQD LLNSAVKLIG DEAWRVRYMA 300
    ADRFSDLASQ FSSNQAYIDE LVQPFLNLCE DNEGDVREAV AKQVSGFAKF 350
    LNDPSIILNK ILPAVQNLSM DESETVRSAL ASKITNIVLL LNKDQVINNF 400
    LPILLNMLRD EFPDVRLNII ASLKVVNDVI GIELLSDSLL PAITELAKDV 450
    NWRVRMAIIE YIPILAEQLG MQFFDQQLSD LCLSWLWDTV YSIREAAVNN 500
    LKRLTEIFGS DWCRDEIISR LLKFDLQLLE NFVSRFTILS ALTTLVPVVS 550
    LDVVTEQLLP FISHLADDGV PNIRFNVAKS YAVIVKVLIK DEAKYDALIK 600
    NTILPSLQTL CQDEDVDVKY FAKKSLAECQ ELLKN 635
    Length:635
    Mass (Da):70,907
    Last modified:October 5, 2010 - v3
    Checksum:i7ED350FB20633DC8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti336 – 3361V → L in AAA35163. (PubMed:1328868)Curated
    Sequence conflicti356 – 3561I → T in AAA35163. (PubMed:1328868)Curated
    Sequence conflicti366 – 3661Q → E in AAA35163. (PubMed:1328868)Curated
    Sequence conflicti611 – 6111C → F in AAA35163. (PubMed:1328868)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98389 Genomic DNA. Translation: AAA35163.1.
    L05146 Genomic DNA. Translation: AAC04941.1.
    BK006935 Genomic DNA. Translation: DAA06972.1.
    PIRiS36718.
    RefSeqiNP_009386.1. NM_001178161.1.

    Genome annotation databases

    EnsemblFungiiYAL016W; YAL016W; YAL016W.
    GeneIDi851217.
    KEGGisce:YAL016W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98389 Genomic DNA. Translation: AAA35163.1 .
    L05146 Genomic DNA. Translation: AAC04941.1 .
    BK006935 Genomic DNA. Translation: DAA06972.1 .
    PIRi S36718.
    RefSeqi NP_009386.1. NM_001178161.1.

    3D structure databases

    ProteinModelPortali P31383.
    SMRi P31383. Positions 35-626.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31750. 58 interactions.
    DIPi DIP-2010N.
    IntActi P31383. 43 interactions.
    MINTi MINT-402163.
    STRINGi 4932.YAL016W.

    Proteomic databases

    MaxQBi P31383.
    PaxDbi P31383.
    PeptideAtlasi P31383.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL016W ; YAL016W ; YAL016W .
    GeneIDi 851217.
    KEGGi sce:YAL016W.

    Organism-specific databases

    SGDi S000000014. TPD3.

    Phylogenomic databases

    eggNOGi NOG247268.
    GeneTreei ENSGT00730000110944.
    HOGENOMi HOG000078539.
    KOi K03456.
    OMAi YMAADRF.
    OrthoDBi EOG7GJ6NN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28828-MONOMER.

    Miscellaneous databases

    NextBioi 968106.

    Gene expression databases

    Genevestigatori P31383.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR000357. HEAT.
    IPR021133. HEAT_type_2.
    [Graphical view ]
    Pfami PF02985. HEAT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS50077. HEAT_REPEAT. 9 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Inactivation of the protein phosphatase 2A regulatory subunit A results in morphological and transcriptional defects in Saccharomyces cerevisiae."
      van Zyl W., Huang W., Sneddon A.A., Stark M., Camier S., Werner M., Marck C., Sentenac A., Broach J.R.
      Mol. Cell. Biol. 12:4946-4959(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes."
      Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H.
      Genome 36:32-42(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    3. "Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the genes in the FUN38-MAK16-SPO7 region."
      Barton A.B., Kaback D.B.
      J. Bacteriol. 176:1872-1880(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry namei2AAA_YEAST
    AccessioniPrimary (citable) accession number: P31383
    Secondary accession number(s): D6VPK2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 123 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 16900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3